밀양23호와 기호벼 교잡 재조합자식계통(RILs)의 품질관련 특성 변이

강현중,김영두,김현순,이영태,은무영,Kang, Hyeon-Jung,Kim, Young-Doo,Kim, Hyun-Soon,Lee, Young-Tae,Eun, Moo-Young 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

통일형인 밀양23호와 자포니카인 기호벼를 교잡한 재조합자식계통을 대상으로 품질관련 특성 변이를 살펴본 결과 공시계통들에 대한 품질 관련 형질들의 변이 분포는 매우 폭 넓고 다양했으며 대부분의 형질에서 연속적인 정규분포를 보였다. 조사된 형질간의 상관관계는 립의 두께에 대하여 립폭, 심백과 복백은 고도의 정의 상관관계를 나타냈으나 심백과 알카러 붕괴도는 고도의 부의 상관관계를 보였다. 단백질 함량과는 아밀로스, Mg/K 비율에서 고도의 부의 상관을 나타냈으며, K와 지방 함량과는 고도의 정의 상관을 보였다. 밥의 물리성에서는 딱딱한 정도를 나타내는 경도와 부착성, 탄력성, 검성, 저작성에서 고도의 정의 상관을 보였다. The rice quality related traits including physico-chemical traits were evaluated with one hundred sixty-four MG RILs derived from the cross between Milyang 23, Indica/Japonica hybrid type, and Gihobyeo, Japonica type. The variation distribution of all traits examined approximately fit normal distribution and transgressive segregants over parents were observed for all traits. The occurrence of such transgression could be associated with the interactions of complementary QTL alleles from two parents. However in this study, it could not be concluded that our results of segregation were due to either complementary gene effects or overdominance of a major gene. These factors should be verified by further studies. Correlations between traits were evaluated by regressing phenotypic values of one trait on those of another traits. There are highly significant correlation between grain thickness with grain width, white core and white belly. But between white belly and alkali digestion value showed highly negative significant correlation. Contents of protein showed highly negative correlation with amylose and Mg/K ratio but showed highly correlation with K and Fat contents. Hardness of cooked rice showed highly correlation with adhesiveness, elasticity, gumminess, chewiness.

가습기 분무 에어로졸을 통한 가습기 살균제의 노출평가

강현중(Hyun-Joong Kang),설휘수(Hwi-Soo Seol),김찬국(Chan-Kook Kim),이종현(Jong-Hyeon Lee),권정환(Jung-Hwan Kwon) 한국환경독성학회 2012 한국독성학회 심포지움 및 학술발표회 Vol.2012 No.5

다수당사자의 소송관계

강현중(Kang Hyeon Jung) 국민대학교 법학연구소 2009 법학논총 Vol.21 No.2

A type of litigation where multiple plaintiffs or defendants (co-litigant) are involved in the same litigation procedure is referred to as joint litigation and the relationship amongst those multiple parties in any joint litigation is referred to as the litigation relationship amongst multiple parties. Joint litigation is widely used because if disputes involving multiple parties are adjudged under one litigation procedure, it will avoid repeated examinations of common issues and permit the settlement of the disputes amongst multiple parties uniformly. There are several types of joint litigation: general joint litigation (Article 66 of the Civil Procedure Act), mandatory joint litigation (Article 67 of the Civil Procedure Act) and preparatory conditional joint litigation (Article 70 of the Civil Procedure Act). The general joint litigation (Article 66) refers to a type of joint litigation where several cases whichshould have been adjudged separately or relatively are consolidated into one litigation procedure. Therefore, although it is one litigation procedure, res judicata applies separately to each claim between co-litigants and the other party to such litigation. The mandatory joint litigation (Article 67) refers to a type of joint litigation where the right(s) or obligation(s) which are the purpose of such litigation are uniformly decided for all of the co-litigants. Therefore, one characteristic of mandatory joint litigation is that res judicata applies to all of the co-litigants uniformly. The mandatory joint litigation is classified into the joint litigation by compulsory joinder where co-litigants are deemed as actual parties to such joint litigation, and the joint litigation by permissive joinder where co-litigants are not deemed as actual parties to such joint-litigation but res judicata applies to all of such co-litigants once the joint litigation is initiated. The preparatory conditional joint litigation (Article 70) refers to a type of joint litigation where the judgment applies to all of the co-litigants to the action but each of the co-litigants may voluntarily dismiss the relevant law suit, or waive, accept or settle a claim because res judicata does not apply to all of such co-litigants. This is an intermediate type between the general joint litigation and the mandatory joint litigation.

種子蛋白質의 SDS-PAGE法에 의한 보리(hordeum vulgare. L)의 品種分類

Hyeon Jung Kang(姜鉉中),Jae Wook Shim(沈載昱) 한국육종학회 1991 한국육종학회지 Vol.23 No.3

This study was carried out to identify the barley cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein. The 23 Korean barley cultivars, 9 covered, 7 naked and 7 malting barely and four foreign cultivars, Carlsberg Ⅱ, Proctor, Jupiter, Vada were used as materials. There was no variation of hordein band patterns among spring, winter and greenhouse cultivation of Doosan # 8 and Doosan # 29. B hordeins were grouped into 11 and C hordeins into 9 and totally grouped into 14 different types by SDS-PAGE of hordeins from 23 Korean barley cultivars and 4 foreign cultivars. The relationships among 27 barley cultivars based on SDS-PAGE band patterns of hordeins were mostly corresponded with the genealogical diagrams of Korean cultivars.

수자원관리를 위한 Core-Platform기반 지능형 통합운영시스템 개발에 관한 연구

권용현(Yong-Hyeon Gwon),최진탁(Jin-Tak Choi),강현중(Hyun-Joong Kang),정승권(Seung-Kwon Jung),장창덕(Chang-Deok Jang) 한국정보기술학회 2015 한국정보기술학회논문지 Vol.13 No.4

Recently, An integrated management technology based on strategic platform is required to response changing environments of water resource management and ICT(information and communication technologies). Smart Water Grid study are underway for intelligent water management of Water resource management and ICT-convergence. Thus, In this study propose framework of using component Modules of various programs to be developed to build of integrated management system for intelligent water management. These frameworks describes the development environment of Core-Platform-based systems for water resources management, by implementing a scenario to implement the integrated operation system, we have developed the system.

연구보문 : 방글라데시 벼 연구개발 현황

미안사이드하산 ( Mian Sayeed Hassan ),강현중 ( Hyeon Jung Kang ),최민규 ( Min Kyu Choi ),구본일 ( Bon Il Ku ),강신구 ( Shin Gu Kang ),박홍규 ( Hong Kyu Park ),칼레쿠자만초두리 ( M. Khalequzzaman A. Chowdhury ),고재권 ( Jae Kwon Ko ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.3

방글라데시의 주식인 쌀은 기후에 따라 크게 3가지 재배시기 (Aus, Aman, Boro)로 나뉘어 연중재배를 하고 있다. 현재까지 개발된 벼 품종은 염저항성, 침수저항성 품종을 포함하여 현재까지 68품종이 개발되었으며, 비료, 살충제, 관개 기술의 발전과 더불어 수량이 증가하고 있다. 각 작기별로 현대적인 품종들이 차지하는 비율은 boro는 96%, T. aman (transplanted aman)는 65%, T. aus는 50%, 그리고 boro-aus 시기에 재배하는 해수침수지역은 20.6% 등 이다. 방글라데시 인구는 2030년까지 1억8천만 명에 달할 것으로 추정되는바, 식량자급을 위해서 4천만 톤의 쌀이 필요할 것으로 예상된다. 따라서 현재 2.8 t/ha인 쌀수량을 4.0 t/ha까지 올려야 하는데, 이는 기후생태별로 염해, 가뭄, 조수, 침수지역 등 각각의 특수한 환경에 적응하는 품종 개발이 필수적이다. 이러한 품종의 개발과 함께 보급 종자 생산, 비료, 작부체계 등 재배기술과 사회경제적인 문제 그리고 환경 문제를 고려한 기술개발이 요구되고 있다. Rice is the major crop and staple food of the majority people in Bangladesh. It is grown almost whole year in the country, where three rice growing seasons are recognized based on climate. Already 68 rice varieties have been developed in Bangladesh including four hybrids, two saline tolerant and two submergence tolerant varieties. Besides, variety development, more than 50 improved technologies on soil, water, fertilizer and cultural practices have been developed. Adoption of modern varieties (MVs) and component technologies like quality seed, fertilizer, pesticides and irrigation resulted in significant yield increase. MVs coverage in boro season (winter irrigated) are around 96% followed by 65% in T. aman (transplanted aman) and 50% in the direct seeded and transplanted aus and 20.6% in the tidal wet land boro-aus saline area. The lowest MV area (14%) is in the T. aman tidal wet land, weakly saline area. Bangladesh population is expected to reach 189.8 million by 2030 and to feed these peoples, almost 40.0 million tons of rice will be required. To feed the increasing population, average yield of rice will have to increase from 2.8 t/ha to 4.0 t/ha. It would be essential to evolve high yield potential rice varieties for favorable ecosystems and agro-ecological constraints areas like saline, drought, tidal and submergence, high temperature and biotic stresses. In addition to variety development, improved production practices, quality inputs specially the seeds, fertilizers and increasing cropping intensity are needed as well as socio-economic including dietary diversity and environmental issues must also be addressed.

SNP 마커를 이용한 벼 흰잎마름병 저항성 선발 효율 증진

신운철,백소현,서춘순,강현중,김정곤,신문식,이강섭,한장호,김현순,Shin, Woon-Chul,Baek, So-Hyeon,Seo, Chun-Sun,Kang, Hyeon-Jung,Kim, Chung-Kon,Shin, Mun-Sik,Lee, Gang-Seob,Hahn, Jang-Ho,Kim, Hyun-Soon 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.4

본 연구는 흰잎마름병 K1 레이스에 감수성인 상주찰벼와 저항성인 HR13721-53-3-1-3-3-2-2를 인공교배하여 육성된 F2, F3를 재료로 하천 Kl 레이스에 대한 저항성 검정과 SNP 마커를 이용한 유전자형 분석 및 저항성과의 연관성을 분석하였다. Kl 레이스에 대한 저항성 검정 결과 $F_2,\;F_3$에서 각각 이론적 분리비인 3:1, 1:1의 분리비를 나타냈으며 SNP 마커를 이용한 유전자형 분석은 16PFXa1 primer를 이용하여 유전자를 증폭한 후 Eco RV 제한효소 처리하여 다형성을 분석하여 저항성 및 유전자형을 확인할 수 있었다. K1 레이스에 대한 저항성 검정과SNP마커를 이용한 유전자형의 연관분석 결과 저항성과 마커간에 연관성이 일치하였으며, 특히 SNP 마커를 이용한 유전자형 분석에서는 K1 레이스에 대한 저항성 검정에서 알 수 없었던 $F_2$ 개체가 동형접합체인지 이형접합체인지를 판별할 수 있어 저항성 품종 육종을 위한 선발 효율을 높일 수 있었다. Discovery of single nucleotide polymorphisms (SNPs), including small insertions and deletions, is one of the hot topics in genetic research. The most common type of sequence variant consists of single base differences or small insertions and deletions at specific nucleotide positions. Significance of SNPs in rice is increasing for genetic research, positional cloning and molecular breeding. $F_2$ 170 lines and $F_3$ 194 lines derived from Sangjuchalbyeo/HR13721-53-3-1-3-3-2-2 Were used for Searching SNP markers related to bacterial blight resistance. Sangjuchalbyeo is susceptible to bacterial blight, but HR13721-53-3-1-3-3-2-2 has Xa1 gene resistant to bacterial blight. Individual lines were inoculated with $K_1$ race of bacterial blight and resistant or susceptible was evaluated after 3 weeks from inoculation. The genotypes of population were analysed by PCR-RFLP for SNP marker developing. The segregation of $F_2\;and\;F_3$ population showed almost 3:1, 1:1 ratio, respectively. Analysis of genotype using SNP marker is capable of confirming resistance for $K_1$ race and genotype through amplifying the gene using 16PFXal primer and digested the PCR product with Eco RV. There were close relation between resistance test for $K_1$ race and SNP marker genotype. Especially, DNA analysis using SNP marker is capable of judging homozygote/heterozygote in $F_2$ population compared with resistant test for Kl race. So, it seems to improve the selection efficiency in disease resistant breeding.

고메이신 함유 옥수수수염 추출물의 유전독성학적 안전성 연구

하애화(Ae Wha Ha),강현중(Hyeon Jung Kang),김선림(Sun Lim Kim),김명환(Myung Hwan Kim),김우경(Woo Kyoung Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.9

본 연구에서는 고메이신 함유 옥수수수염 추출물의 유전독성에 대한 안전성을 규명하고자 세균에서의 복귀돌연변이 유발성, 염색체 이상, 마우스 골수세포에 있어서 소핵실험을 수행하였다. 세균에서의 복귀돌연변이 유발성 여부는 Salmonella Typhimurium의 히스티딘 요구성 균주인 TA100, TA1535, TA98 및 TA1537의 4개 균주와 대장균 Escherichia coli의 트립토판 요구성 균주인 WP2 uvrA를 이용하여 대사활성계 적용(+S9 Mix) 및 비적용(-S9 Mix)하에서 유전 손상을 측정한 결과 모든 균주에서 대사활성계 적용 및 비적용 시 옥수수수염 추출물(5,000, 1,666.67, 555.56, 185.19, 61.73 μg/plate)에서 복귀돌연변이 평균 집락수의 변화 및 농도 의존적인 증가는 관찰되지 않았다. 염색체 이상 실험에서 대사활성계 적용 및 비적용 6시간 처리군(최고농도 1,250 μg/mL)과 대사활성계 비적용 24시간 처리군(최고농도 250 μg/mL)에서 이상 중기상 발현 빈도의 증가 및 음성대조군에 비하여 통계적으로 유의성이 확인되지 않았다. 마우스 골수세포에서의 소핵실험에서는 모든 투여용량의 옥수수수염 추출물(0, 500, 1,000, 2,000 mg/kg)에서 다염성 적혈구 중 소핵다염성 적혈구의 출현 빈도 및 총 적혈구에 대한 다염성 적혈구의 출현 빈도가 음성대조군과 비교하여 유의한 차이가 없었으므로 2,000 mg/kg 옥수수수염 추출물의 섭취는 마우스 골수세포의 소핵 유도에 영향을 주지 않는 것으로 판단된다. 결론적으로 세균에서의 복귀돌연변이 실험, 염색체 이상 실험 및 생체 내에서의 마우스 골수세포에서의 소핵시험을 통하여 본 실험 조건에서 고메이신 함유 옥수수수염 추출물은 유전독성을 유발하지 않는 것을 확인하였다. In this study, a battery of genetic-toxicity studies on corn silk extract with high maysin content were performed according to internationally accepted protocols. In a mutation test using Salmonella Typhimurium TA1535, TA1537, TA98, and TA100, the number of mutant colonies did not significantly increase up to a maximum concentration of 5,000 μg/plate in the presence or absence of the S9 metabolic activation system. In the chromosome aberration test using Chinese hamster lung fibroblasts, negative results were observed in the concentration up to 1,250 μg/mL of corn silk extract. In the micronucleus test using ICR mice, incidence of polymorphonuclear erythrocytes with a maximum concentration of 2,000 mg/kg corn silk extract did not show any significant difference compared to the negative control group. Based on these results, the test substance, con silk extract, did not influence genotoxicity.

국내 육성된 향미 품종의이앙시기별 이화학적 특성 및 향기성분 비교 분석

조준현,송유천,이광식,최식원,이미자,장기창,김현영,강현중,박기도,서우덕,Cho, Jun Hyun,Song, You Chun,Lee, Kwang Sik,Choi, Sik Won,Lee, Mi Ja,Jang, Ki Chang,Kim, Hyun Young,Kang, Hyeon Jung,Park, Ki Do,Seo, Woo Duck 한국환경농학회 2017 한국환경농학회지 Vol.36 No.3

1. 본 연구의 향미의 품종별, 이앙시기별 이화학적 특성 평가한 결과 품종간의 차이는 나타나지 않았으며 단백질 함량은 5.8~7.3%로 나타났고 5월 22일 조기이앙시 평균 6.4%로 가장 낮았으며 지방 함량은 조기이앙시 평균 $2.6{\pm}0.1%$, 보통기이앙시 평균 $2.5{\pm}0.1%$, 만기이앙시 평균 $2.2{\pm}0.1%$를 나타내어 이앙시기가 빠를수록 높은 경향을 나타내었다. 2. 향미 품종 중 Hyangmibyeo 2 ho가 강하점도의 값이 152.3RVU로 가장 높아 전분의 구조가 호화되기 쉬운 구조적 특성을 가지고 다른 품종에 비해 밥맛이 우수 할 것으로 판단되며 이앙시기는 조기나 보통기 이앙이 좋을 것으로 판단된다. 3. 향미 품종, 이앙시기별 향기성분 정량 분석 비교에서는 향기성분 등 중 대표적으로 2-acetyl-1-pyrroline (2AP)를 지표성분으로 정하여 정량분석 한 결과 품종 중에는 Aromi가 $41.7{\sim}66.7{\mu}g/100g$으로 가장 높은 2AP의 함량을 나타내었고 이앙시기의 영향은 모든 품종에서 각각 보통기이앙시 평균 $37.4{\mu}g/100g$, 만기이앙시 $31.5{\mu}g/100g$ 조기이앙 시 평균 $37.4{\mu}g/100g$ 순으로 나타났다. 최적 이앙시기는 생산력 검정 등의 다양한 요소를 고려해 볼 때 6월 5~20일 사이에 이앙하는 것이 최적으로 판단된다. BACKGROUND:The Aromatic rice which is characterized by the flavor of Nurungji when cooked rice, and consumption is increasing recently. The purpose of this study was to investigate the physicochemical characteristics and aroma components of five aromatic rice cultivars according to transplanting time. METHODS AND RESULTS: Quantitative analysis of protein, fat, fatty acid and essential amino acid for five aroma rice cultivars(Hyangmibyeo 2 ho, Aromi, Mihyang, Aranghyangchal, Heughyang)and transplanting time was analyzed by crude protein analyzer, gas chromatography (GC), liquid chromatography (LC) and viscosity analysis was done by using rapid viscosity analyzer (RVA). The content of 2-acetyl-1-pyrroline (2AP) was determined by gas chromatography mass spectrometer. (GC-MS) As a result, the average protein and lipid contents were 6.5% and 2.4%, respectively. The content of essential amino acid showed the highest content at 104.4mg/g. There was no significant change in normal nutrients during the transplanting time. By RVA, cv.Hyangmibyeo 2 ho showed the highest peak and total setback viscosities and lowest breakdown viscosity in early transplantation. The content of 2AP in flavor varieties and transplanting time was quantitatively analyzed by GC-MS. Among the cultivars, Aromi showed the highest 2AP contents at $66.7{\mu}g/100gin$ normal transplanting time. CONCLUSION: cv.Aromi and Hyangmibyeo 2 ho were excellent physicochemical properties and 2AP components contents amongaromatic rice cultivars tested. Theiroptimaltime to transplant was at the beginning of June in the area of Miryang.

남부지역 논에서 사료맥류, 조생종 벼 및 하파귀리를 활용한 삼모작 작부체계 연구

송태화,박태일,박형호,조상균,오영진,장윤우,노재환,박광근,강현중,Song, Tae-Hwa,Park, Tae-Il,Park, Hyong-Ho,Cho, Sang-Kyun,Oh, Young-Jin,Jang, Yun-Woo,Rho, Jea-Hwan,Park, Kwang-Geun,Kang, Hyeon-Jung 한국초지조사료학회 2014 한국초지조사료학회지 Vol.34 No.4

이 연구는 남부지역 논에서 동계 사료맥류, 하계 조생종 벼, 추계 하파귀리 등 삼모작 재배기술을 확립하기 위해실시하였다. 월동 사료작물을 조생종 벼인 조평벼의 이앙시기에 맞춰 4월 30일 1차 수확한 처리구와 사료맥류의 수확기인 5월 30일에 수확한 처리구 모두 곡우호밀이 각각 생초수량 32.0톤/ha과 42.3톤/ha, 건물수량이 5.8톤/ha과 16.5톤/ha로 다른 작물에 비해 높은 수량을 보였다. 하계 조평벼는 5월 6일에 1차 이앙한 처리구보다 6월 4일에 2차 이앙한 처리구에서 등숙비율이 떨어지고 수량도 약 22% 감소하여 조기 이앙이 유리한 것으로 나타났다. 틈새작물로 재배한 하이스피드 귀리는 8월 28일에 1차 파종한 귀리는 10월 10일에 출수하였지만 9월 2일에 입모중으로 2차 파종한 귀리는 출수도 하지 못하였으며 1차 파종에 비해 건물수량과 TDN 수량 모두 50%를 못 미치는 결과를 나타내어 파종이 빠를수록 유리한 것을 알 수 있었다. 따라서 남부지역에서 삼모작을 하기 위해서는 호밀을 재배하여 4월 30일 전에 수확한 후 조평벼의 조기 이앙과 하이스피드 귀리의 적기 파종이 쌀 수량과 조사료 수량 확보에 유리한 것으로 사료되었다. This experiment was undertaken to develop triple cropping systems for winter cereal crops for forage, early maturing rice and oats, and to select a winter forage crop in order to determine rice transplanting time at paddy fields in the southern region. Also, the productivity and feed value of the resulting forage crops were examined. When winter cereal crops used for forages are first harvested at the early maturing rice transplanting period, and again harvested during the winter forage crop harvesting period, the fresh yield and dry matter yield of rye were 32.0, 42.3 ton/ha and 5.8, 16.5 ton/ha, respectively, demonstrating higher yields than other winter crops. The early maturing rice, 'Jopyeong', transplanted on June 4 had a lower percentage of ripened grain compared to those transplanted on May 6, and milled rice yield transplanted on June 4 was also decreased by 22%. Thus, the results showed that early transplanting was profitable. Regarding the oats grown during the fall cropping season, the heading date for the oats sown first was on October 10, but the heading was not observed in those sown later. Dry matter yield and TDN yield of the second sowing was less than 50% compared to the first. Consequently, rye may be the most suitable winter forage crop for triple cropping systems. Early transplanting of 'Jopyeong' after rye harvesting before April 30 in addition to timely sowing of oats in the fall season would be profitable for rice and forage production using triple cropping systems in the southern region.