당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이(Sung Yi Kang),서광식(Kwang Sik Seo),팽정령(Jeong Ryung Paeng),우정택(Jeong Taek Woo),김성운(Sung Woon Kim),양인명(In Myung Yang),김진우(Jin Woo Kim),김영설(Young Seol Kim),김광원(Kwang Won Kim),최영길(Young Kil Choi) 대한내과학회 1994 대한내과학회지 Vol.47 No.2

N/A Introduction: The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appers to be determined essentially by regulation of the rate of enzyme sythesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Methods: Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Results: Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. But glucokinase activity was recuced in the liver of daibetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normogycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. Conclusion: These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expressiom.

음양오행론에서 『회남자(淮南子)』와 『오행대의(五行大義)』의 위상과 상이점

강성인 ( Sung In Kang ),김만태 ( Man Tae Kim ) 단국대학교 동양학연구원 2016 東洋學 Vol.62 No.-

This study conducted comparative research on Huainanzi, which can represent Yin-yang and Five Elements in the Qin Dynasty(秦代) and the Han Dynasty(漢代), and Wuxingdayi in the Sui Dynasty(隋代). Huainanzi including Shicheukhun(時則訓) mentioned the Ruling with Times-Theory, contains a variety of the Theory of Yin-yang and Five Elements in Cheonmunhun·Jihyeonghun· Jusulhun(天文訓·지形訓·主術訓), etc. So the Theory of Yin-yang and Five Elements is the most developed among literature up to the Han Dynasty. Wuxingdayi is the representative record ofthe Thory of Yin-yang and Five Elements that organizied and compiled the Yin-yang and FiveElements of ancient times generally. In the Theory of Yin-yang and Five-Elements discussed in Huainanzi, the Sheng·Geuk of the Five Elements and most of Wangsanghyususa(旺相休囚死) and ‘Putting things into Five Phases’ are similar with those of Wuxingdayi. However, Ganhap(干合) and Jihap(支合) theories, which were not exactly identified in Huainanzi, were established in Wuxingdayi. In Huainanzi, the Sheng(生) and Geuk(剋) theory was completed but the Hap(合) and Chung(沖) theory was not established, while in Wuxingdayi, the Sheng·Geuk·Hap·Chung theory was defined and it was developed to the Hyeong(刑) and Hai(害) theory. In the former, Haihap(會合) of the Earth(土) was called Osulin(午戌寅), which was arranged to Jinsulchukmi(辰戌丑未) in the latter. In Huainanzi, the period during which the Earth governs a year was not defined, thus it was described in various ways such as Sagyewolseol(四季 月說)·72Ilgyehaseol(72日季夏說)·30Ilgyehaseol(30日季夏說). But in Wuxingdayi, Jinmisulchukwol (辰未戌丑月) referring to Sagye (四季) was designated as the month when the Earth prevailed.

만성신부전 환자의 신세뇨관에서의 전해질 조절

고정곤 ( Jeong Gon Ko ),이강휴 ( Kang Hyu Lee ),김인희 ( In Hee Kim ),이식 ( Sik Lee ),김원 ( Won Kim ),강성귀 ( Sung Kyew Kang ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.1

목적 : 만성신부전 환자가 말기신부전에 이를 때까지 세포외액량과 전해질이 정상인의 혈잘량과 전해질치가 거의 유사하게 유지되는 것에 대한 연구가 동물실험에서 진행되어 오고 있으나 사람에서의 연구 (한국)는 극히 제한적이어서 저자들은 만성 신부전증 환자 19명을 대상으로 잔여 신장의 세뇨관에서 전해질 조절이 어떻게 되는가의 기전을 관찰하고자 하였다. 방법 : 만성신부전의 19명의기저질환은 당뇨병성 신중 (42.1%), 만성 사구체신염 (31.6%), 역류성 신수종 (10.5%), IgA신중 (5.3%), 신증후군 (5.3%)의 순이였고, 평균연령은 54.6세이고 남자 13명, 여자 6명이었다. 대조군은 23명으로 평균 50.1세이고 남자19명, 여자 4명으로 여러가지 신기능의 지표를 검사하였다. 결과 : 만성신부전시 혈철 Na^+ 평균 농도는 139.9±3.2 mEq/L이고 대조군은 141.1±2.1 mEq/L로 유의하게 감소하는 경향이고, 혈청 K^+ 평균농도는 4.7±0.8 mEq/L로 정상대조군 4.2± mEq/L에 비하여 유의하게 증가하는 경향이었다. 사구체여과율은 9.7±6.3 mL/min이고 대조군의 75.9±42.9 mL/min에 비하여 유의한 감소가 있었다. 만성신부전시 소변내 배설되는 Na^+의 평균농도는 89.9±31.6 mEq/L이고 정산대조군은 175.4±68.5 mEq/L로서 유의하게 감소하였으며 (p<0.05), thqusso qotjfehlsms K^+ 평균농도는 24.3±14.8 mEq/L으로 대조군 32.1±22.7 mEq/L보다 감소하는 경향이였다. 소변 osmolality는 평균 397.7±119.1 mOsm.kg로서 정상대조군 610.6±210.9 mOsm/kg에 비하여 유의하게 감소하였는데 이는 소변 농축력이 감소 하였음을 반영한다. 만성신부전에서 단위 네프론당 전해질 배설량을 알아보기 위해 측정한 fractional excretion of Na^+ (FENa)의 평균치는 3.4±5.4%이고 정상대조군은 0.6±0.6%로서 유의하게 증가하였으며 fractional excretion of K^+ (FEK)의 평균치는 15.4±20.8%로서 정상 대조군의 2.2±22.9% 보다 유의하게 증가하였다. Transtubular K^+ gradient (TTKG)는 7.1±6.9%로서 정상대조군 3.2±2.8%보다 유의하게 증가하였다. 혈액 가스 분석 소견은 만성신부전시에는 pH가 7.4±0.1로서 대조군 7.4±0.02에 비하여 변화가 없었고, HCO_3^- 는 18.9±2.8mEqL으로 정상대조군 24.1±2.2mEq/L보다 유의하게 감소하였다. 결론 : 만성신부전 환자의 혈액내 전해질 특히 Na^+ 농도는 감소하고 K^+와 C1^- 농도는 증가 하였으나, 정상치에 가깝게 유지되고 있었으묘, 이는 FENa와 FEK의 유의한 증가로 보아 신세뇨관에서의 Na^+는 재흡수 억제에 기인하고, 또 혈장내 K^+의 증가는 세뇨관에서 K^+ 분비의 증가를 초래하였는데 이는 TTKG의 활성화에 기인한 것으로 사료되었다. Purpose : In chronic renal failure (CRF), extracellular fluid (ECF) volume is maintained close to normal, often until end-stage renal disease is imminent. This remarkable feat is accomplished by increase in fractional excretion of sodium (FENa) in inverse proportion to the decline in glomerular filtration rate (GRF). Many researchers have carried out to try to indentify in animal study but human study was not done in Korea. Methods : The study is an investigation of the changes of plamse and urine electrolytes and FENa and fractional excretion of potassium (FEX) in 19patints (13 men and 6 women) with chronic renal failure. Ages of 19 patients were average 54.6 yearold (range, 29-74 years). Underlying renal disease of the CRF was 42,1% in diabetic nephropathy, 31.6% in chronic glomerulonephritis, 10.5% in hydronephrosis with ureter reflux, and 5.3% in IgA nephropathy. Results : In CRF, plasma Na^+ is decreased significantly from normal control 141±2.1 mEq/L to 139.9±3.2 mEq/L and GER from 75.9±42.9 mL/min to 9.7±6.3 mL/min, but plasma K^+ is increased significantly from 4.2±0.4 mEq/L to 4.7±0.8 mEq/L. In CRF however, urine Na^+ is decreased significantly from normal control 175.4±58.5 mEq/L to 89.9±31.6 mEq/L and osmolality from 610.6±210.9 mOsm/kg to 397.7±119.1 mOsm/kg, but urine K^+ is devreased tendency from control 32.1±22.7 mEq/L to 24.3±14.8 mEq/L FENa, FEK, and transtubular potassium gradient (TTKG) on CRF were 3.4±5.4% 15.4±20.8% 7.1±6.9% each and 0.6±0.6%, 2.2±2.3% 3.2±2.8% on normal persons. The difference between CRF and normal control in FENa, FEK, TTKG and osmolar clearance were statistically significant. Conclusion : These results suggest that renal tubular cells of CRF were responsible for the decreased Na^+ and K^+ reabsorption and enhance K^+ secretion.

비모란 액아를 기내에서 증식시킨 후 기외 접목시 적정 자구크기 구명

강성해(Kang Sung Hae),윤재길(Yun Jae Gill),박인태(Park In Tae),조창휘(Cho Chang Hi),유창재(Yu Chang Jae) 한국원예학회 1997 한국원예학회 학술발표요지 Vol.15 No.2

Welding Gap Detecting and Monitoring using Neural Networks

Sung-In Kang(강성인),Gwan-Hyung Kim(김관형),Sang-Bae Lee(이상배),Han-Ho Tack(택한호) 한국지능시스템학회 1998 한국지능시스템학회 학술발표 논문집 Vol.8 No.2

만니톨 투여에 의한 대사성 알칼로시스

강성귀(Sung Kyew Kang),김원(Won Kim),김인희(In Hee Kim) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.4

Mannitol is an osmotic diuretic as a useful agent in the treatment of a variety of clnical conditions. In persons with normal renal function, the mannitol is almost excreted by kidney. Although various studies of the effectiveness of mannitol for the cerebral edema have been reported, but there are still few re- ports on acid-base disorder. This study is based on acid-base and electrolyte changes after the intravenous infusion of hypertonic mannitol for the purpose of preventing cerebral edema. Mannitol were intravenously infused with 300-900 mL for 90-minutes (group A), 1,200-2,600 mL for 24-hours(group B) and 3,200-4,900 mL for over 24-hours(group C) each. Each blood sample was drawn for gas analysis, and electrolytes through arterial line before and after mannitol infusion. In group A, blood pH is increased significantly from baseline level 7.43±4 0.07 to 7.46±0.04, and plasma HCO3 25.3±2.1 mEq/L to 28.9±2.9 mEq/ L each, but plasma K is decreased significantly from baseline level 4.3±0.6 mEq/L to 3.70.8 mEq/L In group B, blood pH is increased significantly from baseline level 7.420.02 to 7.47±0.06, and plasma HCO3 25.2±1.8 mEq/L to 29.1±2.9 mEq/L each, but plasma K is decreased significantly from baseline level 4.2±0.3 mEq/L to 3.8±0.5 mEq/L. In group C, blood pH is increased significantly from baseline level 7.41±0.01 to 7.52±0.04, and plasma HCO3 24.9±1.2 mEq/L to 27.7±2.5 mEq/L each, but plasma K is decreased significantly from baseline level 4.20.1 mEq/L to 3.9±0.2 mEq/L These results clinically used intravenous infusion of mannitol could induce metabolic alkalosis associated with hypokalemia, regardless of its dosage.

자연재해에 의한 관망사고 복구 매뉴얼

강성원 ( Sung Won Kang ),공명식 ( Myeong Sik Kong ),박은주 ( Eun Zoo Park ),이준형 ( Joon Hyung Lee ),이현동 ( Hyun Dong Lee ),최종인 ( Jong In Choi ),곽필재 ( Pil Jae Kwak ),강성원 ( Sung Won Kang ),이준형 ( Jun Hyung Lee ),이현 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

Production of $Ser^{125}$-Interleukin-2 by Site Directed Mutagenesis

강성만,곽주원,권종범,김성완,이인영,이선복,윤혜영,함경수,한문희,나도선,Kang, Seong-Man,Kwak, Ju-Won,Kwon, Jong-Bum,Kim, Sung-Wan,Lee, In-Young,Lee, Sun-Bok,Yun, Hye-Young,Hahm, Kyung-Soo,Han, Moon-H.,Na, Doe-Sun 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.3

아미노산 서열 125번 위치의 아미노산인 시스테인을 세린으로 치환한 Inter1eukin-2를 특정 위치 변이방법에 의하여 대장균을 이용하여 생산하였다. $Ser^{125}$-IL-2는 IL-2 의존성인 배양세포에 대하여 $Cys^{125}$-IL-2와 유사한 성장촉진효과를 나타내었다. $Ser^{125}$-IL-2의 생산 수율은 $Cys^{125}$-IL-2에 비하여 1.5배 이상 높았다. Human Interleukin-2 (IL-2) posessing a serine in place of cystein at the amino acid sequence position 125 has been produced in E. coli by site directed mutagenesis. The activity of purified $Ser^{125}$-IL-2 was more than 2.5 times higher than that of $Cys^{125}$-IL-2 as determined by the growth promoting effect on IL-2 dependent cell line. The yield of production of $Ser^{125}$-IL-2 was at least 1.5 times higher as compared to that of $Cys^{125}$-IL-2.

A Study on Detecting and Monitoring of Weld Root Gap using Neural Networks

강성인,김관형,Kang Sung-In,Kim Gwan-Hyung The Korea Institute of Information and Commucation 2006 한국정보통신학회논문지 Vol.10 No.7

일반적으로 용접 Root 캡은 여러 가지 용접 결함들 중에 용접 품질을 저하시키는 중요한 요인 중의 하나이다. Gas metal arc welding (GMAW)에서 root 갭은 용접 전류, 아크 전압, 용적률 등과 같은 여러 가지 용접 파라미터들에 영향을 미친다. 그러나 용접 공정의 비선형성 때문에 root 갭과 많은 용접 파라미터들 사이의 관계를 분석하기가 힘들다. 그리고 아크센서를 사용하였을 경우, 감지된 신호에 대한 신호처리가 어렵지만 가격이 저렴하고 자동화하기가 쉬우므로 현재의 산업공정에서 대부분 아크센서가 사용되고 있다. 지금까지 언급된 여러 가지 어려운 문제점과 아크센서의 특정 때문에 본 논문에서는 GMAW에서 root 갭을 검출할 수 있는 적당한 용접 파라미터들을 선정하고, root 캡과 선정된 파라미터들의 관계를 인식할 수 있는 신경회로망을 이용하여 root 갭 검출 시스템을 설계하였다. 또한, 용접 품질의 검사에 용접 비드형상이 중요한 요인이다. 따라서 본 논문에서는 신경회로망으로 용접 파라미터와 용접 비드형상과의 관계를 인식하여, 용접 품질을 추정하고 용접 파라미터들의 효과를 분석할 수 있는 용접 비드 형상의 실시간 모니터링 시스템을 제안하여 여러 실험 데이터들을 기반으로 한 시뮬레이션을 통해 제안된 시스템이 root 갭을 검출하고 또한 용접 비드 형상을 실시간으로 모니터링이 가능함 보여준다. Weld root gap is a important fact of a falling-off weld quality in various kind of weld defect. The welding quality can be controlled by monitoring important parameters, such as, the Arc voltage, welding current and welding speed during the welding process. Welding systems use either a vision sensor or an Arc sensor, both of which are unable to control these parameters directly. Therefore, it is difficult to obtain necessary bead geometry without automatically controlling the welding parameters through the sensors. In this paper we propose a novel approach using neural networks for detecting and monitoring of weld root gap and bead shape. Through experiments we demonstrate that the proposed system can be used for real welding processes. The results demonstrate that the system can efficiently estimate the weld bead shape and detect the welding defects.

Zigbee를 이용한 휴대형 헬스케어 시스템 구현에 관한 연구

강성인,김관형,Kang, Sung-In,Kim, Gwan-Hyung 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.9

헬스케어는 정보통신기술을 활용하여 원거리에서 대상자들의 건강상태에 대한 모니터링과 홈 기반의 보건의료 서비스를 제공하는 것이라 할 수 있다. 따라서 헬스케어 서비스는 응급상황 대처를 위한 경보서비스와, 진료자 및 가족에게 대상자와 댁내 환경을 모니터링 할 수 있는 원격지원 서비스로 분류할 수 있다. 여기서, 일반적인 유선 기반의 고정형 생체계측 시스템에서는 두 가지 서비스를 위해서, 실시간으로 높은 신뢰성을 갖는 데이터를 전송하는데 한계가 있다. 그러므로 대상자가 공간적으로 구속을 받지 않고 자유롭게 이동하면서 생체신호 계측 및 응급경보가 가능하도록, 무선 네트워크 환경에서 구현되는 휴대용 헬스케어 시스템을 구현하고자 한다. Recently there are some trends to construct smart home system infrastructure depending upon the development of Information and Communication Technology. Also requirements of the Ubiquitous Healthcare Systems at home which can monitor the status of health continuously are increased rapidly comparing with hospitals. Healthcare service can be divided into two categories. The first one is Alarm Service that can be used for the emergency status and the other one is Remote Support Service which can monitor the patient including home environments and give those diagnosis information to medical office or to his family. Generally wired networks and fixed healthcare measuring system have some limits to transmit reliable realtime based information for both categories described above comparing with portable monitoring system. Getting over the inefficiency we will design and implement portable healthcare system under the wireless Zigbee network environments.