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난자 세포질 내 정자 주입술시 부고환 및 고환 정자의 체외수정능력에 관한 비교 연구
성기청,강문주,김희선,오선경,구승엽,서창석,김석현,최영민,김정구,문신용,Sung, Ki-Cheong,Kang, Moon-Joo,Kim, Hee-Sun,Oh, Sun-Kyung,Ku, Seung-Yup,Suh, Chang-Suk,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.2
Objective: This study was carried out to compare the clinical outcomes of intracytoplasmic sperm injection (ICSI) in patients with obstructive azoospermia according to sperm retrieval site and technique; microsurgical epididymal sperm aspiration (MESA), percutaneous epididymal sperm aspiration (PESA), testicular sperm extraction by open biopsy (TESE). Methods: The outcomes of ICSI and IVF-ET were evaluated and compared among 3 groups. Seventy three men suffering from infertility due to obstructive azoospermia had 107 ICSI cycles using MESA (21 cycles in 15 patients), PESA (26 cycles in 17 patients) and TESE (60 cycles in 41 patients). Results: In the clinical outcomes in patients undergoing ICSI with epididymal or testicular sperm, there were no significant differences in fertilization rate (66.1% vs. 60.5%), cleavage rate (94.9% vs. 97.6%), cumulative embryo score (CES) (51.3 vs. 58.8), implantation rate (7.9% vs. 6.1), and clinical pregnancy rate per ET (30.4% (14/46) vs. 25.4% (15/59)) between both groups. Also, in the clinical outcomes in ICSI patients using MESA, PESA, TESE, there were no significant differences in fertilization rate (61.8%, 69.4%, 60.5%), cleavage rate (92.1%, 97.3%, 97.6%), CES (38.1, 52.0, 58.8), implantation rate (9.5%, 6.6%, 6.1%), and clinical pregnancy rate per ET (35% (7/20), 26.9% (7/26), 25.4% (15/59)) among 3 groups. Conclusion: When compared with MESA or TESE, PESA, the clinical outcomes were similar in ICSI patients with obstructive azoospermia whatever the origin or the technique of sperm retrieval. However, we considered PESA is more time-saving and cost effective for ICSI in patients with obstructive azoospermia.
Duchenne형 근이영양증의 착상전 유전진단을 위한 Duplex-nested PCR과 Triplex-nested PCR 방법의 연구
김성아 ( Sung Ah Kim ),윤정아 ( Jung Ah Yoon ),강문주 ( Moon Joo Kang ),김희선 ( Hee Sun Kim ),오선경 ( Sun Kyung Oh ),구승엽 ( Seung Yup Ku ),김석현 ( Seok Hyun Kim ),최영민 ( Young Min Choi ),김정구 ( Jung Gu Kim ),문신용 ( Shin 서울대학교 인구의학연구소 2007 人口醫學硏究論集 Vol.20 No.-