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Zoogloea ramigera 115와 Zoogloea ramigera l15SLR의 중금속 흡착능 비교
이한기,배우철,진욱,정욱진,이삼빈,정병철,Lee, Han-Ki,Bae, Woo-Chul,Jin, Wook,Jung, Wook-Jin,Lee, Sam-Pin,Jeong, Byeong-Chul 한국미생물·생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.1
Capsular polymer인 zooglan을 생산하는 Z. ramigera 115와 수용성 slime polymer 생산 변이주인 Z. ramigera l15SLR의 중금속 제거능을 비교하였다. 중금속 5종($Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$, $Fe^{2+}$)의 최저 생장저해농도는 두 균주 모두 유사하였다. $Cd^{2+}$을 첨가하지 않은 경우에 비해 $Cd^{2+}$ 500ppm을 첨가한 액체 배지에서의 생체량은 Z. ramigera 115는 24%, Z. ramigera l15SLR은 50%로 감소하였고 동일한 생체량에 대하여 Z. ramigera 115SLR이 Z. ramtgera 115보다 $Cd^{2+}$을 1.5배 더 제거하였다. 세포와 polymer를 분리하여 중금속 흡착능을 비교한 결과 polymer가 세포보다 높은 흡착량을 나타냈으며 최적 중금속 흡착 pH는 7.5였다. Langmuir 흡착등온선과 Freundlich 흡착등온선을 분석한 결과 $Cd^{2+}$에 대하여 Z. ramigera l15SLR polymer는 Z. ramigera 115 및 이온 교환 수지보다 높은 최대흡착량과 흡착안정성을 보여서 홉착제로서 유용할 것으로 판단된다. Heavy metal removal by Z. ramigera 115 and soluble slime polymer producing mutant Z. ramigera 115SLR was investigated. Both strains showed similar tolerance against $Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$ and $Fe^{2+}$. When cells were cultivated in the presence of 500 ppm $Cd^{2+}$, the mutant strain removed 1.5 fold more metal than the wild type did at same biomass. Metal adsorption capacities were in the order of Z. ramigera l15SLR polymer > Z. ramigera 115 polymer > Z. ramigera 115 cell >Z. ramigera l15SLR cell. The optimum pH for metal adsorption was 7.5. Langmuir and Freundlich isotherms indicated that Qmax and 1/n of Z. ramigera l15SLR polymer were 164.2 mg $Cd^{2+}$/g dw and 0.496, respectively. These results showed that the polymer of Z. ramigera l15SLR could be used as an effective metal adsorbate.
급성 관동맥증후군에서 예후평가를 위한 NT-proBNP, troponin 1, hs-CRP의 유용성
오병천 ( Pyung Chun Oh ),한승환 ( Seung Hwan Han ),정욱진 ( Wook Jin Chung ),강웅철 ( Woong Chol Kang ),서일혜 ( Yiel Hea Seo ),엄영실 ( Young Sil Eom ),문찬일 ( Chan Il Moon ),봉정민 ( Jeong Min Bong ),신미승 ( Mi Seung Shin ) 대한내과학회 2009 대한내과학회지 Vol.77 No.2
Background/Aims: Elevated levels of N-terminal pro-B-type natriuretic peptide (NT-proBNP), troponin I, and high-sensitivity C-reactive protein (hs-CRP) are each associated with higher rates of death and recurrent myocardial ischemia in patients with acute coronary syndrome (ACS). We evaluated the prognostic value of NT-proBNP and a multi-marker risk approach with the simultaneous assessment of NT-proBNP, troponin I, and hs-CRP in patients with ACS. Methods: We included 277 patients who were admitted for ACS between January and December 2006. We measured NT-proBNP, troponin I, and hs-CRP within 24 hours of the onset of symptoms. Patients were followed for a an of 559 days for cardiovascular events, including death, new myocardial infarction, heart failure, or rehospitalization for ACS. Results: NT-proBNP was the most powerful predictor of clinical outcome among the biomarkers (HR 3.65, 95% CI 2.11-6.30), followed by the peak troponin I and hs-CRP (HR 2.08, 95% CI 1.12-3.87; HR 1.99, 95% CI 1.18-3.37, respectively), but not the baseline troponin I. A multi-marker risk approach with the simultaneous assessment of NT-proBNP, hs-CRP, and peak troponin I was significantly associated with cardiovascular events, especially the presence of three positive biomarkers (adjusted HR 4.20, 95% CI 1.39-12.67). Conclusions: NT-proBNP is the most powerful, independent predictor of clinical outcome among the cardiac biomarkers. Since the peak troponin I level provides more prognostic information than the baseline level, follow-up measurement of troponin I may be warranted for risk stratification. The multi-marker risk approach appears to have better prognostic performance than any marker in isolation. (Korean J 77:200-210, 2009)
김호경 ( Ho Kyung Kim ),김원기 ( Won Ki Kim ),김주한 ( Joo Han Kim ),이정우 ( Jeong Woo Lee ),정욱진 ( Wook Jin Chung ),권오상 ( Oh Sang Kwon ),김주현 ( Ju Hyun Kim ) 대한내과학회 2021 대한내과학회지 Vol.96 No.3
간폐증후군은 폐내 혈관 확장으로 발생하는 간경변의 합병증 중의 하나로 동맥혈 산소 포화도 저하를 유발한다. 호흡곤란이 환자가 호소하는 가장 흔한 증상이며 입위 시 악화소견을 보인다. 신체 검사에서 곤봉지와 청색증이 발견되기도 하며 유일하게 간이식이 현재까지는 가장 효과적인 치료법으로 알려져 있다. 본 증례에서는 호흡곤란을 호소하는 간경변 환자에서 곤봉지를 동반한 간폐증후군을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Hepatopulmonary syndrome is a rare lung complication of liver cirrhosis, caused by pulmonary microvascular vasodilation that induces abnormal arterial oxygenation. Typical findings on physical examination are finger clubbing and cyanosis. Dyspnea is a common symptom and is worse in the upright position. Contrast echocardiography is a useful diagnostic test. Currently, the only effective treatment is liver transplantation. We report the case of a woman with cirrhosis who has hepatopulmonary syndrome with finger clubbing, confirmed by contrast echocardiography. The patient is waiting for a liver transplant. (Korean J Med 2021;96:236-240)
배우철,정욱진,정병철,장승욱 明知大學校 自然科學硏究所 1997 자연과학논문집 Vol.15 No.-
오염하천인 중랑천에서 중금속 내성세균 J7을 분리하여 생육과 카드뮴 흡착특성을 조사하였다. 회분배양에 미치는 카드뮴의 영향을 조사한 결과 카드뮴을 첨가하지 않았을 경우에는 비생장속도 0.32hr¹유도기 1시간, 카드뮴 106ppm의 경우에는 0..6hr¹, 12시간, 카드뮴 265ppm의 경우에는 0.04hr¹, 24시간으로 카드뮴 농도 증가에 따라 비생장속도의 감소와 유도기의 증가를 나타냈다. 카드뮴 제거량은 106.2㎎/g DCW, 191.6㎎/g으로 카드뮴 농도의 증가에 따라 높게 나타났다. 최적 카드뮴 흡착 pH는 8.0으로 이때 78㎎/g/ DCW의 효율을 나타냈다. 이처럼 생장시의 제거량이 생장후의 단순흡착량보다 더 높게 나타나는 것으로 미루어 생장시에는 카드뮴 첨가에 의한 유도과정에 의해 단순흡착량보다 더 높게 나타나는 것으로 미루어 생장시에는 카드뮴 첨가에 의한 유도과정에 의해 단순흡착외의 다른 중금속 제거기작도 작용하는 것으로 여겨진다. J7에 의한 카드뮴 흡착은 Langmuir 흡착등온식에 적합하였고 Scatchard Plot에서 직선을 나타낸 것으로 보아 단층 흡착이고 카드뮴의 농도 변화에 흡착능이 큰 영향을 받지않는 안정된 상태를 갖는 것으로 보인다. 낮은 농도에서 흡착제의 효율을 확인하는 Freundlich 흡착등온식에서 흡착강도 (1/n)이 0.3178로 흡착제로의 사용가능성이 높고 흡착능(K) 은 1.331로 높게 나타났다. Cadmium tolerant bacterium J7 was isolated from Jungrang stream and investigated in terms of growth and cadmium adsorption properties. The specific growth rate of the bacterium was 0.32hr¹at Oppm of cadmium concentration, and the lag phase continued for lhour. But when the cadmium concentration was 106ppm. the specific growth rate and the duration of lag phase were 0.06hr¹ and 12hour, respectively. When initial cadmium concentration was 265ppm, they were 0.04hr¹ and 24hour. This result showed that the specific growth rate decreased but the lag phase increased as the cadmium concentration increased. The quantities of cadmium removed by J7 was 106.2㎎ cadmium/ g dry cell weight at 106ppm and 191.6㎎/g at 265ppm of cadmium. The maximum cadmium adsorption of pre-grown cell was 78 ㎎/g at pH 8.0 The removal capacitiy of growing cell was higher than adsorption capacites of resting cell after growth, and this indicates the addition of cadmium induced another metal -removing mechanism of the cell. Langmuir isotherm and scatchard plot were suitable models to explain the cadmium removal by J7. This indicates that single layer adsorption and the change of cadmium concentration has little effect on adsorption capacity of the bacterium, The Frendlich isotherms was available to measure the adsorption efficiency at low metal concentration. Adsorption strength(1/n) was 0.3178 and capacity(K) was 1.331. This indicates the possiblity of the application of the bacterium as an adsorbate at low metal concentration.