http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
배영훈,안태훈,임성철,박석천,이재창,강남현,배춘식,Bai, Young-Hoon,An, Tae-Hun,Lim, Sung-Chul,Pak, Sok-Cheon,Lee, Jae-Chang,Kang, Nam-Hyun,Bae, Chun-Sik 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
For the induction of arthropathy, 5% hydrogen peroxide($H_2O_2$) was injected for 5 weeks into the intraarticular space of the New Zealand white rabbits to damage articular cartilage. Alginic acid of low molecular weight (2%) made from macromolecular alginate treated with enzyme was administered into articular space at the dose of 5 mg/kg twice a week for 3 and 6 weeks using 1 ml syringe and 26 G needle. Saline was injected for the control. Tissues surrounding the articulation were obtained for the measurements of superoxide dismutase(SOD) activity as a major antioxidant enzyme and malondialdehyde (MDA) as a lipid peroxidation level. Histopathologic examination on the surface of articular cartilage was carried out. Data showed that injection of hydrogen peroxide for 5 weeks had led to the induction of free radical damage and of articular cartilage change as confirmed by microscopic observation. The application of hydrogen peroxide caused a gradual increase in the SODs and MDA. These patterns were similar after 3 and 6 weeks of alginate treatment. Furthermore, microscopic examinations revealed that hydrogen peroxide caused flaking, fibrillation, fissuring, denudation, and hypocellularity in the articular surfaces. In conclusion, lipid peroxidation was demonstrated in the articular cartilage by the administration of hydrogen peroxide in the rabbit model. This lipid peroxidation could be caused by oxygen free radicals. The histologic and enzymatic correlations on lipid peroxidation in the articulation have provided a better understanding of arthropathy. It is possible to take advantage of these findings to evaluate effective alginate dosage more efficiently.
제대동정맥 ECMO를 적용한 염소태아의 혈액가스 및 혈류역학 변화
송창훈(Chang Hun Song),소금영(Geum Young So),이국현(Kook Hyun Lee),박석천(Sok Cheon Pak) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.9
N/A Objectives : The purpose of this study is to establish animal model of extracorporeal membrane oxygenation (ECMO) system that uses membreane type oxygenator and circulation circuit of umbilical artery and vein. Blood gas and hemodynamic changes in the fetal goat undergoing ECMO were also evaluated. Methods : Total 15 pregnant goat had been used to perform extrauterine fetal incubation using ECMO through umbilical artery and vein. Cesarean-section was performed to pregnant goat (35 kg) of 120-130 days of gestation to insert catheters (8 Fr) into the umbilical artery and vein. The tip of inserted catheter's the other end was connected with the circuit system including membrane type oxygenator (Polystan) and roller pump. A total of 300 ml of blood was drawn from donor nonpregnant goat and primed into circuit on the day of surgery. The goat fetus was immersed in a chamber filled with artificial amniotic fluid to monitor blood flow dynamics and blood gas was analyzed. Results : The ECMO system using umbilical cord in the extrauterine incubation of fetal goat was developed and maximum survival of goat fetus was 34.5 hrs (mean survival was 856.6±688 min). Oxygen tension (PO2) in umbilical artery and vein were 20.53±2.54 mmHg, 31.03±13.03 mmHg and oxygen saturation (SO2) in umbilical artery and vein were 46.61±18.14 mmHg, 71.56±15.39 mmHg. Mean blood flow was 176±62 ml/min/kg. Conclusion : We suggest that our experimental model as an extrauterine fetal research could be a reasonable method in future advanced studies. However, longterm survival of extrauterine fetus needs more suitable hemodynamic and blood gas condition supported by further researches.
이은봉,박석천 順天大學校 農業科學硏究所 1995 農業科學硏究 Vol.9 No.-
The purpose of this study was to determine the effect of high density lipoprotein (HDL) and low density lipoprotein (LDL) on the growth of porcine embryos in vitro. A total 229 embryos ranging from 4 cell to blastocyst stage were recovered from 12 sexually mature crossbred gilts through midventral laparotomy. Only morphologically normal embryos were randomly allocated into culture in one of the following media : 1) mKRB without lactate and pyruvate (n=65), 2) mKRB plus HDL (n=64), 3) mKRB plus LDL (n=66). They were incubated in an atmosphere of 5% CO_2/, 5% O_2/, 90% N_2. Daily observations stopped when embryos failed to develop for two consecutive observations. In no case did 4-8 cell embryos advance more than 1 developmental stage. Morulas tended to advanced 2 stage when HDL and LDL were added. Blastocysts advanced 1 stage and 2 stage when HDL and LDL were added respectively. The statistical evaluation showed no significant differences (p<0.05) between treatments.
Comparison of the in Vivo Activity of Two Protent Oxytocin Antagonsts on Utrine Activit in the Rat
Bai, Young-Hoon,Song, Chang-Hun,Pak, Sok-Cheon 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2
목적: 비임신 백서에서 옥시토신 길항제인 Antag I과 TT-235의 체내 효과를 비교하기 위하여 시행되었다. 재료 및 방법: 모든 백서들은 약물을 주입하기 위한 목적으로 마취상태하에서 cannula를 jugular vein에 넣었다. 또한 자궁의 수축상태를 측정하기 위해 끝에 풍선이 달리고 안에 물을 채워넣은 또 다른 cannula를 자궁속에 넣었다. 자궁이 수축되는 수치는 10분간의 간격대로 측정되고 계산되었다. 각 옥시토신 길항제를 5㎍의 농도로 백서에 주입한 다음, 5분 후에 100 mU의 옥시토신을 넣고 이후 계속하여 1시간 간격으로 총 5시간 동안 옥시토신 주입을 계속하였다. 결과: 옥시토신 길항제인 Antag I과 TT-235를 주입하고 난 5분 후에 옥시토신을 주고 자궁의 수축상태를 살폈을 때 대조군에 비해 각각 77%와 92%가 억제되었다(P <.05). 2시간이 지난 후에 Antag I은 대조군에 비해 53% 정도의 자궁수축 억제효과를 보였는데 여전히 유효한 차이가 있었다(P <.05). 3시간대부터는 더 이상 대조군과의 차이를 보이지 않은 Antag I에 비해 TT-235는 5시간대까지 계속에서 대조군과 유효한 차이를 보이면서 자궁수축을 억제시켰다. 결론: 옥신토신에 의해 유발된 비임신성 백서의 자궁수축은 TT-235에 의한 억제 효과가 오랫동안 지속되었다. 체내에서의 이와 같은 효과는 산모에서 자궁 조기 진통을 억제하는데 이 약제가 효과적으로 적용될 수 있다는 것을 암시하였다.