약침용 봉독성분 melittin의 영향에 의한 인체 폐암세포의 apoptosis 유도

안창범,임춘우,윤현민,박수진,최영현,Ahn, Chang-beohm,Im, Chun-woo,Youn, Hyoun-min,Park, Su-jin,Choi, Yung-hyun 대한침구의학회 2003 대한침구의학회지 Vol.20 No.5

Objective : To investigate the possible molecular mechanism(s) of melittin as a candidate of anti-cancer drug, we examined the effects of the compound on the growth of human lung carcinoma cell line A549. Methods: MTT, morphological changes, DAPI staining, Western blot, RT-PCR and in vitro prostaglandin E2 (PGE2) accumulation assays were performed. Results: The anti-proliferative effect by melittin treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. Melittin induced apoptotic cell death in a concentration-dependent manner, which was associated with inhibition or degradation of apoptotic target proteins such as ${\beta}$-catenin, poly(ADP-ribose) polymerase(PARP) and phospholipase $C-{\gamma}1(PLC-{\gamma}1)$. Melittin treatment inhibited the expression of cyclooxygenase-2(COX-2) and accumulation of PGE2 in aconcentration-dependent fashion. In addition, Melittin treatment induced the down-regulation of telomerase reverse transcriptase(hTERT) and proto-oncogene c-myc expression of A549 cells. Conclusions: Taken together, these findings suggest that melittin-induced inhibition of human lung cancer cell proliferation is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products, and melittin may have therapeutic potential in human lung cancer.

Bax의 발현증가 및 Caspase의 활성을 통한 봉독약침액 Melittin의 인체폐암세포 Apoptosis 유발에 관한 연구

안창범,임춘우,김철홍,윤현민,장경전,송춘호,최영현,Ahn, Chang-beohm,Im, Chun-woo,Kim, Cheol-hong,Youn, Hyoun-min,Jang, Kyung-jeon,Song, Choon-ho,Choi, Yung-hyun 대한침구의학회 2004 대한침구의학회지 Vol.21 No.2

Objective : To investigate the possible molecular mechanism (s) of melittin as a candidate of anti-cancer drug, we examined the effects of the compound on the growth of human lung carcinoma cell line A549. Methods : Growth inhibitory study, flow cytometry analysis, SDS-polyacrylamide gel electrophoresis and Western blot analysis, RT-PCR and in vitro caspases activity assay were performed. Results : Melittin treatment declined the cell viability of A549 cells in a concentration-dependent manner, which was associated with induction of apoptotic cell death. Melittin treatment down-regulated the levels of Bcl-XS/L mRNA and protein expression of A549 cells, an anti-apoptotic gene, however, the those of Bax, a pro-apoptotic gene, were up-regulated. Melittin induced the proteolytic cleavage and activation of caspase-3 and caspase-9 protease in a dose-dependent manner without alteration of inhibitor of apoptosis proteins family and Akt expression. Western blot analysis and RT-PCR data revealed that the levels of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 were also remained unchanged. Conclusions : Taken together, these findings suggest that melittin-induced inhibition of human lung cancer cell growth is associated with the induction of apoptotic cell death via regulation of several major growth regulatory gene products, and melittin may have therapeutic potential in human lung cancer.

오행침자법(五行鍼刺法) 원리 및 임상응용에 관한 연구 - II 임상응용에 관한 연구

안창범,민영광,김정은,문혁철,송춘호,이장천,신상우,Ahn, Chang-Beohm,Min, Young-Kwang,Kim, Jeong-Eun,Moon, Hyuck-Chol,Song, Chun-Ho,Lee, Jang-Cheon,Shin, Sang-Woo 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.3

Objective: To review the clinical basis of the Sa-Ahm 5 Element acupuncture originated about 360 years ago, papers and books were researched. Methods: Total of 58 books and papers ranging from ancient Huang Di nei jing to modern Bio Medical Acupuncture for Pain Management were investigated to study the clinical practice of it. Results: It could be practiced differently depending upon the viewpoints of diagnosis. They are ranging from theories of the greater-less of Yin and Yang, 7 emotions, organs- visceras, 8 diagnostic criteria, and pulse diagnosis to meridian circulation. Conclusions: It needs to be set clinical guidelines by which to practice Sa-Ahm acupuncture though it is nowadays mostly-used method in accordance with practitioner's viewpoints.

용성산인(龍城山人) 이창원(李昌遠)의 사상학설(四象學說)과 체질침(體質鍼)에 대한 고찰(考察)

안창범,윤현민,허재영,권혜연,Ahn, Chang-beohm,Youn, Byuon-Min,Hea, Jae-young,Kwon, Hae-Yon 대한침구의학회 2002 대한침구의학회지 Vol.19 No.1

Objective : This study was carried out to review Chang-Won Lee's theory of Sasangand acupuncture of constitution. Methods : We have investigated Chang-Won Lee's unknown literature "Medicine of sassang "(四象醫學). Results : Constitutional acupuncture is based on the diversity of human being and medically developed the response variation to disease and acupuncture. The diversity is due to the Organ's Congenital Formation Variation based on human nature and it is categorized as four from physiology, pathology, symptoms, to therapy. Chang-Won Lee's constitutional acupuncture discriminated constitution of comparison palpations of right and left both sides. He insisted that constitution only four type(Sasang), not exist man of To-Hyoung (土形人) in "young -chu"(靈樞) and his constitutional acupuncture all used Jung-kyuck (reinforcement acupuncture method) and Seong-kyuck(reducing acupuncture method) of Sa-am(舍岩)

현훈(眩暈)의 침구치료(鍼灸治療)에 대한 문헌적(文獻的) 고찰(考察)

송춘호,안창범,장경전,윤현민,Song Choon-Ho,Ahn Chang-Beohm,Jang Kyung-Jeon,Youn Hyoun-Min 경락경혈학회 2003 Korean Journal of Acupuncture Vol.20 No.4

Objectives & Methods : This study was to study acupuncture and moxibustion treatments for dizziness, I've got compared and analyzed 41 kinds of books since Nei Jing(內經). It seems that the meridians of Bladder, Gall bladder and Governing vessel were mostly used for them in connection with functions of each meridians. Results and Conclusion : 1. The symptoms of dizziness are illusion, nausea, vertigo, tinnitus and mild headache 2. The meridians of acupuncture points which was used much for dizziness were Bladder meridian, Gall bladder meridian and Governing Vessel 3. The frequency of using acupuncture points in this treatment were P'ung Ji(GB20)-27th, Chok-Samni(S36)-17th, Paek'oe(GV20)-15th, Pungnyung(S40)-9th, Shinmack (B62)-9th.

신세뇨관(腎細尿管) 상피세포(上皮細胞)에서 산화(酸化)로 유발(誘發)된 apoptosis에 대한 호도약침액(胡桃藥鍼液)의 방어효과(防禦效果)

박인범,안창범,장경전,송춘호,윤현민,김철홍,Park, In-bum,Ahn, Chang-beohm,Jang, Kyung-jeon,Song, Choon-ho,Yoon, Hyoun-min,Kim, Cheol-hong 대한침구의학회 2004 대한침구의학회지 Vol.21 No.3

Objective: This study was undertaken to evaluate the role of lipid peroxidation in oxidant-induced apoptosis and effect of JS on the apoptosis in opossum kidney (OK) cells, an established renal proximal tubular cells. Methods : Exposure of cells to 0.1mM tBHP for 2hr did not induce apoptosis, but subsequent incubation in normal culture medium for 18hr after tBHP treatment induced apoptotic cell death which is dependent of tBHP concentration. Results : JS decreased tBHP-induced apoptotic cell death in a dose-dependent fashion and at concentrations higher than 0.01 mg/ml completely prevented the apoptosis. tBHP-induced apoptosis was prevented by the lipid soluble antioxidant N,N'-diphenyl-p-phenylenediamine (DPPD) and water-soluble antioxidant Trolox. tBHP increased lipid peroxidation, which was inhibited by JS and DPPD. tBHP-induced DNA damage was prevented by JS and DPPD. Conclusion : These results indicate that tBHP induces apoptosis through a lipid peroxidation-dependent mechanism and JS exerts the protective effect against the apoptosis by preventing peroxidation of membrane lipids.

뇌졸중(腦卒中) 환자(患者) 형태(形態)에 관(關)한 임상연구(臨床硏究)

윤현민,안창범,송춘호,손인석,장경전,Youn, Hyoun-min,Ahn, Chang-beohm,Song, Choon-ho,Son, In-seok,Jang, Kyung-jeon 대한침구의학회 2003 대한침구의학회지 Vol.20 No.2

Clinical observation was made on 52 cases of Stroke that were confined through brain CT, MRI scan. The Stroke cases wee classified into the following kinds cerebral infarction, cerebral hemorrhage, cerebellar or brain stem infarction, cerebellar or brain stem hemorrhage. And among the 52 cases of Stroke cerebral infarction was noticed in 75.00%, cerebral hemorrhage in 11.54%, cerebellar or brain stem infarction in 9.52%, cerebellar or brain stem hemorrhage in 3.85%. The ratio between males and females was 1.74:1 in the whole groups of Stroke and most cases were over 60 of age. As the time of hospitalization, most patients hospitalized from 1 day after stroke to 7 days after stroke. And as the course of hospitalization, most patients hospitalized first. Among the preceding disease at the onset of Stroke hypertention was noted in 32.69%, and deabetes mellitus or heart problem was noted frequently(15.39%). Electrocardiography findings were as follows: The normal was noted in 53.85%, the abnormal in 46.15%. And as the abnormal, left ventricular hypertrophy was noted in 17.54%. The predisposing factors or conditions at the onset of brain infarction were usually initiated during the time of sleeping and those of brain hemorrhage chiefly during the time of exercising like overwork or walking etc. It was noted that smoking a pack of cigarette showed highest disease rate(33.33%) among the average of smoking amount of one day in case of man. Prior to attack, the most chiefly complain was dyspnea or discomfort on chest region. And 30.70% of patients had no previous sign. There were a large number of recurrent cases. The first attack was noted in 71.15%, the 2nd attack in 23.08%, the 3rd attack in 5.77%.

Effect of Juglans sinensis Dode extract on chemical hypoxia-induced cell injury in human glioma cells

윤현민,허재영,안창범,Youn, Hyoun-min,Heo, Jae-yeong,Ahn, Chang-beohm The Korean AcupunctureMoxibustion Medicine Society 2003 대한침구의학회지 Vol.20 No.2

이 논문(論文)은 활성 산소(ROS)의 작용(作用)을 규명하고 호도약침액(胡桃藥鍼液)이 인간의 신경교종 세포인 A172에서 화학적(化學的) 저산소증(低酸素症)으로 유발된 세포 사멸에 대해 효능이 있는지를 연구(硏究)한 것이다. 화학적(化學的) 저산소증(低酸素症)은 세포내 미토콘드리아의 전자 수송을 방해하는 antimycin A를 가진 배양세포에 의해 유발(誘發)하였다. 화학적(化學的) 저산소증(低酸素症)에 노출된 세포(細胞)는 시간과 그 양에 따라서 세포 사멸의 결과(結果)가 다르게 나타난다. 화학적 저산소증에 의해서 ROS의 생산이 증가하는데 이것은 $H_2O_2$ 소거(消去) Catalase(과산화수소를 물과 산소로 분해하는 효소)에 의해 방지(防止)된다. Catalase는 화학적 저산소증에 의해 유발(誘發)된 세포 사멸을 방지하는데 비해 DMTU는 효과적이지 않다. 지질(脂質)에 녹는 산화방지제 DPPD와 물에 녹는 산화방지제 Trolox는 세포사멸을 방지하는데 효과(效果)가 없다. 호도약침액(胡桃藥鍼液)은 그 양(量)에 의존적으로 저산소증에 의해 유발된 세포 사멸을 방지하는 효과가 있다. 즉 화학적 저산소증으로 유도된 ROS의 발생을 막고, $H_2O_2$로 유도된 세포사멸을 방지하는데 이것은 화학적 저산소증과 $H_2O_2$의해 유도된 세포사멸에 대해 호도약침액(胡桃藥鍼液)이 방지효과(防止效果)가 있다는 것을 의미한다. 이러한 결과(結果)들은 $H_2O_2$가 지질 과산화와는 무관한 메카니즘으로 저산소증(低酸素症)으로 유발(誘發)된 세포사멸을 중재하고, 따라서 호도약침액(胡桃藥鍼液)은 지질막의 과산화를 방지하기 보다는 ROS를 직접적으로 소거(消去)함으로써 방지 효과가 있다는 것을 의미한다. 더구나 화학적(化學的) 저산소증(低酸素症)은 caspase와 무관한 메카니즘으로 apoptosis를 유발(誘發)한다.

기능성 두통에 대한 황련해독탕약침(黃連解毒湯藥鍼)의 임상적 연구

김홍기,윤현민,안창범,Kim, Hong-Ki,Youn, Hyoun-Min,Ahn, Chang-Beohm 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.3

Objective : The aim of this study was to investigate the effect of HHT(Hwangryunhaedoktang Herbal-Acupuncture) on functional headache. Methods : 26 clinical experiment participants who experienced functional headache were gathered. The qualified patients were classified in two group, Normal saline injection group and Hwangryunhaedoktang Herbal-Acupuncture group. NS group was injected normal saline and HHT group was injected HHT at GB20, GB21, LI4. Treatment was applied 4 times every second day. The effects of both groups were analyzed using VAS scores and BPI(Brief Pain Inventory). Result : The VAS pain scores improved in both groups. the BPI scores decreased only in HHT group. The decrease rate of VAS scores and BPI scores was larger in HHT group. Conclusion : These results provided that HHT group is more effective than NS group. For clearly comparing the Effect of Hwangryunhaedoktang Herbal-Acupuncture on functional headache, it is need more sample's number and more treatment's duration.

『영추(靈樞)』, 『소문(素問)』, 『난경(難經)』에 나타난 오수혈(五輸穴)의 개념에 대한 고찰

안철우,민영광,안창범,장경전,김철홍,송춘호,윤현민,Ahn, Chul-Woo,Min, Young-Kwang,Ahn, Chang-Beohm,Jang, Kyung-Jun,Kim, Cheol-Hong,Song, Choon-Ho,Yoon, Hyun-Min 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.1

In $\ulcorner$Yingshu(靈樞)$\lrcorner$, $\ulcorner$Sumun(素問)$\lrcorner$ and $\ulcorner$Najing(難經)$\lrcorner$, there are differences in viewing FiveSu-point(五輸穴) and its practical application in treatment. The following conclusions are induced from these different point of views. 1. Fundamental features of FiveSu-point are shown in$\ulcorner$Yingshu(靈樞)$\lrcorner$ 2. In $\ulcorner$Internal classic(內經)$\lrcorner$ and $\ulcorner$Najing(難經)$\lrcorner$, it seems attachment that FiveSu-point is identical to the Five Elements, but there are differences in using the Five Elements sangsyang-sangguk in treatment. 3. There are clear differences between $\ulcorner$Internal classic(內經)$\lrcorner$ and $\ulcorner$Najing(難經)$\lrcorner$ in attachment of FiveSu-point and Fourseasons. Differences are shown in each chapter of $\ulcorner$Yingshu(靈樞)$\lrcorner$. 4. Different application of FiveSu-point's various cases is shown in $\ulcorner$Yingshu(靈樞)$\lrcorner$, $\ulcorner$Sumun(素問)$\lrcorner$ and $\ulcorner$Najing(難經)$\lrcorner$. 5. Different application of FiveSu-point in $\ulcorner$Yingshu(靈樞)$\lrcorner$, $\ulcorner$Sumun(素問)$\lrcorner$ and $\ulcorner$Najing(難經)$\lrcorner$ is due to different historical background.