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Aspergillus candidus F1484 균주가 생산하는 항진균 화합물의 분리 및 특성
김성옥,이소영,김성규,손광희,김영국,문석식,복성해 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5
Candida albicans의 효모형태에 대해 활성을 나타내는 항진균 물질의 탐색 중에 Aspergillus candidus F1484 균주의 발효액으로부터 항진균 화합물 F1484를 단리하고 ethyl acetate 추출, silica gel column chromatography, ODS column chromatography 및 preparative HPLC를 행하여 분리정제하였다. F1484 화합물은 EI-MS, ^13C-, ^1H-NMR, DEPT, HMQC 및 HMBC에 의한 구조분석을 행한 결과 이 화합물은 항진균 물질인 chloroflavonin과 동일한 구조를 나타내었으며 효모형태의 Candida albicans에 대한 항진균 활성뿐만 아니라 여러 종류의 인체 종양 세포주에 대해서도 세포독성을 나타내었다. In the course of screening for the antifungal compounds against Candida albicans, an antifungal compound (F1480) was isolated from the culture broth of Aspergillus candidus F1484. Isolation and purification of compound F1484 were performed using ethyl acetate extraction, silica gel column chromatography, ODS column chromatography, and preparative HPLC. The structure of compound F1484 was determined by the spectroscopic analyses of EI-MS, ^13C-, ^1H-NMR, DEPT, HMQC, and HMBC. This compound appeared to have a structure of antifungal agent, chloroflavonin. In addition to antifungal activities against the yeast phase of Candida species, compound F1484 showed cytotoxic effect against various human tumor cell lines.
BOK, SONG HAE,Cho, Kyung Hyun,Choi, Myung Sook,Park, Yong Bok 경북대학교 유전공학연구소 1996 遺傳工學硏究所報 Vol.11 No.1
This study describes a stable and simple method for the measurement of cholesteryl ester transfer protein (CETP) activities using reconstituted HDL and LDL as substrates. Apolipoproteins(apo) A-I and -B were purified from hog plasma by a new strategy without ultracentrifugation and delipidation. A simple two-step column chromatography was administered. In the first step of phenyl-sepharose CL-4B column chromatography, hydrophobic plasma proteins were isolated. The most hydrophobic proteins bound to the column appeared to be apo A-I and apo-B. Contaminant proteins were efficiently eliminated from the sample by washing the column with 0.3M NaCl containing buffer after loading the plasma on the column. Two pure proteins showing each single band on SDS-PAGE of apo A-I and apo-B were individually obtained by a subsequent gel filtration column chromatography(Sephadex G-200). This two-step purification was simple and inexpensive compared to the ultracentrifugation and/or delipidation method that are most commonly used. Reconstituted high-density lipoproteins(HDL) and low-density lipoproteins(LDL) were prepared using the purified apo A-I and -B, respectively. When these artificially prepared HDL and LDL were used in the assays for CETP as the cholesteryl ester(CE) donor and acceptor respectively, the specific transfer of CE increased up to two fold compared to that used the native HDL and LDL.
BOK, SONG HAE,Park, Yong Bok,Jang, Moon Kyoo,Yoon, Woo Hyun 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Biologically active human plasma cholestetyl ester transfer protein (CETP) was directed to produce as a fusion protein with glutathione-S-transferase (GST) in recombinant vaccinia virus-infected mammalian cells. Complementary DNAs for GST snd CETP were 6gated side by side, and introduced into vaccinia viral transfer vectors. The viral vectors were transfected into CV-1 cells infected previously with wild-type vaccinia viruses. Recombinant viruses were generated by the homologous recombination and selected with BUdR and X-gal in human TK^- 143B cells. The DNA from the selected virus was amplified and expressed in the CV-1 cells. The GST/CETP fusion protein (86 kDa) was identified on the SDS-PAGE followed by Western blot analysis using polyclonal antibody against the C-terminal active region of CETP fused with GST. The virus-infected cell lysates were loaded on the glutathione-Sepharose affinity column and the CETP was eluted from the column by cleavage with thrombin while the GST/CETP was bound on the column. The purified CETP showed biological activity when subjected to a CETP assay.
Song, Jae Min,Woo, Bok Hee,Lee, Ji Hye,Yoon, Sanggyeong,Cho, Youngseuk,Kim, Yong-Deok,Park, Hae Ryoun MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.10
<P>Chemotherapy is not a first-line therapy for oral squamous cell carcinoma (OSCC), which is the most common type of oral cancer, because most OSCC shows resistance to chemotherapeutic reagents. Inflammatory signals are suggested to be associated with chemoresistance as well as carcinogenesis in many different cancers, and thus chronic periodontitis, the most common chronic inflammatory disease of the oral cavity, could modulate responsiveness to chemotherapeutic agents used against oral cancer. This study was performed to define the role of chronic periodontitis in oral cancer progression and to determine the responsiveness of oral cancer to a chemotherapeutic reagent. First, we quantified the tumor growth rate and changes in serum cytokine profiles of mice administered Porphyromonas gingivalis, a major pathogen of chronic periodontitis. Compared with uninfected mice, the mice that were chronically administered P. gingivalis showed increased resistance to paclitaxel and a decreased tumor growth rate. In addition, P. gingivalis-treated mice exhibited higher serum levels of interleukin-6 (IL-6) than uninfected mice. Furthermore, the sensitivity of tumor xenografts to paclitaxel in mice administered P. gingivalis was dramatically increased when the mice were administered ibuprofen, an anti-inflammatory drug which supports the modulatory effect of periodontal pathogen-induced inflammation in chemoresistance.</P>