양극성 정동장애와 혈청 Inositol-1-phosphatase 활성도 변화

조현주,김영철,우행원,연규월,조기승 대한생물치료정신의학회 1998 생물치료정신의학 Vol.4 No.2

Objects : Several lines of possibilites have been oroposed about the etiology of bipolar attective disorder in biological, genetic and psychosocial aspect. The purpose of this study is to inspect the etiology of bipolar affective disorder in biological aspect by measuring the changes of serum inositol-1-phosphatase activity and investing a causal relationship between clinical symptoms and serum inositol-1-phosphatase activities in patients with bipolar affective disorder. Methods : The subjects were 18 inpatients who met DSM-IV diagnostic criteria for bipolar I affective disorder, manic episode and 15 normal controls. Inositol-1-phosphatase activities were measured in serum by using spectrophotometer. Clinical symptoms of patients were assessed by YMRS at admission day and after 2 weeks lithium treatment. Results : 1) Inositol-1-phosphatase activities of the patients were increased to about 2 times as compared with those of normal controls. And the activity levels were decreased gradually to the normal at 14 days. 2) Young's Mania Rating Scale(YMRS) scores were decreased significantly by lithium treatment. 3) Statistical significance could not be found between the change of YMRS scores and the levels of inositol-1-phosphatase activity. Conclusion : In this study, statistical significance could not be found between clinical improvement and inositol-1-phosphatase activities, but the fact that lithium treatment improve the clinical manifestation shown by YMRS score changes, and reverse the increased inositol-1-phosphatase activities suggests the strong association between pathophysiology of bipolar affective disorders and inositide metabolism.

슬관절부 골절에 대한 임상적 고찰

김기용,조우신,빈성일,백승일 울산대학교 의과대학 1992 울산의대학술지 Vol.1 No.1

The knee joint is the largest weight bearing joint. As the fractures of Knee joint in volve the articular surface and the surrounding soft tissues, permanent disabilities such as deformities, joint stiffness, instability and post-traumatic arthritis may ensue following treatment. Many authors have recommended various methods of treatment for these fractures. Recently, however, a trend toward internal fixation has become evident and good results have been reported by several authors. This study is to present 40 cases of knee joint fractures which were treated at Asan Medical Center from May 1989 to April 19992. The results were as follows ; 1. The causes of injury were traffic accident(60%), fall down(12.5%), slip down(12.5%) and sports injury(10%). 2. There were 23cases in 22 males and 17 females in series and average was 46.8 years ranging from 19 years to 82 years. 3. In femoral condylar fractures, the most frequent type was C2(8 cases ; 42.2%) and in tibial condylar fractures, B1(7 vases ; 35.5%) by the A.O. classification. 4. 7 Cases were treated by conservative method 32 cases by operative method and 1 cases by amputation. 5. Satisfactory results were obtained 73.3% in femoral condylar fractures and 88.2% in tibial condylar fractures by operative treatment. We concluded that satisfactory results were obtained from operative treatment with procedures of anatomical reduction, rigid fixation and early joint motion.

생쥐에서 KCN의 독성과 해독제 평가에 관한 연구

이철영,조기승 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

시안화합물(CN­)의 독성에 대한 해독제 평가 실험에 앞서, 먼저 생쥐를 사용하여 구강, 복강 및 피하 주입에 의한 시안화 칼륨(KCN)의 반치사량(??)을 24시간후의 생존수에 의해 측정하여 보았더니, 각각 70.mg/kg, 8.6mg/kg 및 8.8mg/kg 등을 나타냈으며, 결과로 보아 구강투여가 다른 경로에 비해 독성이 심한 것으로 관찰되었다. 시안화합물의 독성에 대한 해독 효과를 보기위해 여러 가능한 Sulfur-공여체와 몇 몇 해독 효과가 알려진 화합물들을 생쥐에 투여하여 KCN의 ??에 대한 해독 배율을 측정하여 보았더니, 시험해 본 화합물중에서 NaNO₂, Na₂S₂O₃ 및 Cysteine 등이 2배 이상의 해독율을 나타냈으며, NaNo₂+Na₂S₂O₃ 혼용 투여에서는 5.4배율의 해독 효과를, NaNo₂+Na₂S₂O₃+Vit.?? 혼용투여에서는 8.6배의 해독 상승율을 보이므로 단독 투여보다는 혼용 복합 투여가 더 효과적임을 나타내 주었다. 한편 S-공여 화합물인 6-thioguanosine과 2-thioxanthine은 1.4배의 해독율을 보였고, thiodiethanol은 1.6배의 해독율을 보여 이들 화합물이 CN­ 독성 해독에 있어서 rhodanese에 대한 S-공여체로서 얼마간 작용하고 있음을 보여주었다. Before the evaluation of antidotal efficacy on cyanide poisoning in mice, fifth percent of lethality(??) determined on 24 hr mortality after administration of KCN through on oral, intraperitoneal cavity and subcutaneous injection was exhibited 7.0mg/kg, 8.6mg/kg, 8.8mg/kg respectively. It demonstrated that oral administration of KCN was more severe in toxicity than other routes Several Sulfur-donor and other konwn compounds were tried in order to examine the detoxication potency against ?? of KCN toxicity in mice. Among them, single pretreatment of each NaNo₂, Na₂S₂O₃and Cysteine showed the over two fold detoxication potency. On the other hand, complex pretreatments of two of three compounds, NaNo₂+Na₂S₂O₃ or NaNo₂+Na₂S₂O₃+Vit.B exhibited more effectiveness than single pretreatment as showing the potency ratio of 5.4 and 8.6, respectively. While, S-donor compounds, 6-thioguanosine and 2-thioxanthine exhibited 1.4 fold detoxication potency, and thiodiethanol showed 1.6 fold detoxication. From these results such thiol compounds were somethat active as S-donor to rhodanese reaction in CN-detoxication.

신경 세포에서 Ca-channel의 비활성에 관한 연구

이영식,조기승 漢陽大學校 基礎科學硏究所 1988 基礎科學論文集 Vol.7 No.-

신경세포의 bursting 기작을 설명하기 위하여 기존에 제안된 Ca-activated Potassium channel 대신 Ca channel을 사용하여 기존의 model과 유사한 결과를 얻었다. 이들의 Phase Plane에서의 행동도 유사함을 보여준다. We have modified slow oscillation dynamics in Plant Nerve cell model in order to incorperated the Ca chnnael incativation mechanism. The calcium activated potassium channel has been replcaed with leak and voltage dependent calcium channel with and calcium inactivated voltage gated calcium channel. The simulation results with our model resemble the experimental results and Plant model's. The phase plane behavior of our model is also similar to the Plant model's behavior. It suggest that the calcium channel inactivation mechainsm may be a possible mehcanism for the slow oscillation dynamics in membrane potential oscillation in neuron cell body.

사람 적혈구 막에 있어서 Carnitine acyltransferase 효소에 관한 연구

박중근,김응렬,조기승 漢陽大學校敎養學部 1976 硏究論文集 Vol.- No.1

사람 적혈구막에서 palmitoyl carnitine을 생성하는 효소계의 존재를 확인하였는데 이 효소계에 의해 palmitoyl coenzyme A와 DL-Carnitine(methyl-14C)으로부터 palmitoyl carnitine-14C이 생합성됨을 고찰하였고, 한편 이 효소계에 의해 화학적으로 합성한 palmitoyl-14C-carnitine이 palmitic acid-14C과 carnitine으로 가수분해됨을 확인하였다. 이 효소계에 의해서나 또는 화학적 방법에 의해 만들어진 palmitoyl carnitine은 적혈구막을 용해하며 이때 막에 부착된 효소계인 Adenosine Triphosphatase나 Glucose-6-phosphatase등이 활성화됨을 발견하였다. An enzyme system in human erythrocyte membrane was found to catalyze the formation of palmitoyl carnitine-14C from palmitoyl coenzyme A and carnitine-(methyl-14C). It was also confirmed that the same enzyme acts on the hydrolysis of palmitoyl-14C-carnitine into palmitic acid-14C and carnitine. Palmitoyl carnitine formed from this enzyme system or other chemical method solubilized the erythrocyte membrane and stimulated significantly the membrane bound enzymes, such as Adeno-sine Triphosphatase (ATPase) and Glucose-6-phosphatase (G-6-Pase) tried.

Centipede Scolopendra subspinipes multilans L. Koch로부터 항균력이 있는 Centipedin의 추출, 정제 및 특성연구

김기태,최진성,민천홍,정일엽,박경배,조기승 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-

항균력이 있는 화합물인 Centipeding을 지네 Scolopendra subspinipes multilans L. Koch로부터 지용성 유기용매로 추출하였고, silicic acid column과 high S cation exchange chromatography 및 reverse-phase HPLC 등을 사용하여 고순도로 정제하였다. Gram-positive bacteria인 Staphylococcus aureus ATCC 6538P 및 gram-negative인 Klebsiella pneumoniae ATCC 8308 균주에 대하여 강한 항균력을 나타내었다. Dragendorff 시약에 의한 발색반응을 시행한 결과 Centipedin의 aromatic ring에는 nitrogen이 없음을 확이하였고 IR, Mass, NMR 분석 등에 의하여 Centipedin의 분자량은 162.1, 화학적 구조는 hydroxyl기와 carbonyl기를 함유하는 -hydroxyisocumarin으로 확인되었다. UV-spectrum 검색결과에 의하면 최대 흡수파장이 243 ㎚임을 확인하였고, melting point는 122℃이었으며, 실온에서 3개월간 방치된 Centipedin이 여전히 항균활성 및 화학적 구조를 유지하여 물리화학적 및 생물학적으로 안정한 화합물임으 알 수 있었다. The compound which has antibiotic activity was extracted and purified from centipede Scolopendra subspinipes multilans L. Koch by diethyl ether, silicic acid column and high S cation exchange chromatography followed by reverse-phase HPLC. This compoung named "Centipedin" showed antibiotic activity aganist microorganisms such as Gram^(+) -bacteria Staphylococcus aureus ATCC 6538P and Gram^(-) -bacteria Klebsiella pneumoniae ATCC 8308. Dragendorff test showed that there is no aromatic nitrogen within the Centipedim. The molecular weight of the Centipedin was 162.1 daltons as determined by mass spectrometry. The instrumental analysis of IR and NMR techniques revealed the molecular structure of Centipedim as 8-hydroxyisocoumarin. The UV-spectrum of Centipedin showed maximum absorption at 243 ㎚, and melting point was 122℃. Centipedin was stable at room temperature for three months in appropriate buffers.

대장균에 있어서 Acylphosphatidyl Glycerol 의 생성 기작에 관한 연구

Cho, Key Seung,Hong, Seung Duk,Lee, Kang Suk,Chang, Chung Soon 생화학분자생물학회 1980 BMB Reports Vol.8 No.1

Cell free extracts of E. coli B were found to catalyse the conversion of phosphatidyl glycerol to acylphosphatidyl glycerol (semilysobis phosphatidic acid). It has been found that phosphatidyl glycerol acted both as an acyl acceptor and a donor. Phosphatidyl ethanolamine was also found to be a good acyl donor. These results are explained by an internal and external transacylation reaction.

Solution Structure of an Active Mini-Proinsulin, M2PI: Inter-chain Flexibility is Crucial for Insulin Activity

Cho, Yoon-Sang,Chang, Seung-Gu,Choi, Ki-Doo,Shin, Hang-Cheol,Ahn, Byung-Yoon,Kim, Key-Sun 생화학분자생물학회 2000 Journal of biochemistry and molecular biology Vol.33 No.2

M2PI is an active single chain mini-proinsulin with a 9-residue linker containing the turn-forming sequence 'YPGDV' between the B- and A-chains, but which retains about 50% of native insulin receptor binding activity. The refolding efficiency of M2PI is higher than proinsulin by 20-40% at alkaline pH, and native insulin is generated by the enzymatic conversion of M2PI. The solution structure of M2PI was determined by NMR spectroscopy. The global structure of M2PI is similar to that of native insulin, but the flexible linker between the B- and A-chains perturbed the N-terminal A-chain and C-terminal B-chain. The helix in the N-terminal A-chain is partly perturbed and the ${\beta}$-turn in the B-chain is disrupted in M2PI. However, the linker between the two chains was completely disordered indicating that the designed turn was not formed under the experimental conditions (20% acetic acid). Considering the fact that an insulin analogue, directly cross-linked between the C-terminus of the B-chain and the N-terminus of the A-chain, has negligible binding activity, a flexible linker between the two chains is sufficient to keep binding activity of M2PI, but the perturbed secondary structures are detrimental to receptor binding.

다발성 안면골 골절의 정복

조승호,김기헌,김병우 대한이비인후과학회 1977 대한이비인후과학회지 두경부외과학 Vol.20 No.1

電氣 가오리 Acetylcholinesterase 酵素의 特性에 關한 硏究

曺基勝 漢陽大學校 環境科學硏究所 1982 環境科學論文集 Vol.3 No.-

남해안에서 나는 전기가오리의 발전기관으로부터 Acetylcholinesterase 를 추출 정제하였다. Sepharose 4B Affinity Column Chromatography 에 의해 정제한 결과 예민한 단일 peak를 나타냈고, Specific activity 는 2,700으로 68배의 정제도를 나타냈다. Acetylcholinesterase의 최적 pH는 7.3 과 7.6 사이로 이 pH 전후에서는 활성도의 감소추세를 보였고, 여러 가지 Buffer 용액에 대한 효소활성도를 시험한 결과 시험한 Buffer 용액 중 Tris-HCI Buffer 용액이 가장 높은 활성도를 보였다. 25℃ 에서 여러 안정제에 대한 효과를 보았을 때, 0.01% Casein 과 0.05 M Mgcl₂를 함유한 Tric-HCI Buffer 용액에서 효소활성도가 가장 안정함을 보여 Casein 과 ?? 이 안정효과가 있음을 확인하였다. Acetylcholinesterase는 매우 낮은 농도의 유기인산화합물인 parathion 과 같은 농약에 매우 민감한 저해현상을 나타냈다. Acetylcholinesterase was extracted and purified from electric organs of Electrophorus Japonica inhabited a southern coast. Acetylcholinesterase showed a single sharp peak by affinity column chromatography and has a specific activity, 2,700, representing a 68 fold purification over the homegenate. The optimum pH range of this enzyme was between from 7.3 to 7.6, and decreased outside this pH range. In the several buffer solutions tested, the highest enzyme activity was obtained with Tris-HCI buffer. It is certified that 0.01% casein and 0.05M Mgcl₂have a stabilizing effect on enzyme activity at 25℃ in Tris-HCI buffer. Acetylcholinesterase was inhibited sensitively by a low concentration of organic phosphorus compound, suchas, parathion.