치근 천공 치료 재료의 생체친화성의 비교

강민경,배인호,고정태,황윤찬,황인남,오원만 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.3

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner(Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5〔(sulfenylamino) carbonyl〕2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA. 이번 연구는 치근 천공의 치료 재료인 white mineral trioxide aggregate (MTA)를 흔히 사용되는 calcium hydroxideliner(Dycal^(R), glass ionomer cement (GIC), 그리고 MTA와 유사한 성분을 가진 Portland cement와 세포독성 실험으로 생체 친화성을 평가하는 것이다. 세포독성의 정도는 MG-63 세포를 이용해 주사전자 현미경적 관찰과 수용성 tetrazolium salt를 이용한 흡광도를 측정 (XTT assay)하여 평가하였다. SEM 관찰에서, 1일과 3일째 모두에서 GIC와 MTA, Portland cement표면에서는 잘 부착된 세포를 보여주었다. 반면에, Dycal 표면의 세포들은 둥글고 부착되지 않은 양상을 보여주었다. XTT assay에서는 Dycal을 제외한 모든 재료에서 유사하게 높은 세포 활성도를 보여주었으며, 이는 SEM 관찰 소견과 일치하였다. 이번 연구는 MTA가 생체친화적인 재료라는 견해를 뒷받침한다. 또한 Portland cement와 GIC에서도 MTA와 유사한 세포반응을 보여주었다.

Pd 촉매 하에서 알릴 디올의 아릴화 연구 : 방향족 히드록시 케톤 및 페닐 치환된 알릴디올의 합성 Highly Selective Synthesis of Phenyl-Substituted Allylic Diols

강석구,정경윤,박찬희,남궁은영,김태현 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.2

The coupling reaction of iodobenzene with allylic diols in the presence of Pd(OAc)_2 and nBu_3P as catalysts using K_2CO_3 as base afforded the phenyl-substituted allylic diols. However, under the same reaction conditions with Et3N as base, phenyl-substituted α-hydroxy ketone was obtained.

진네트정(세푸록심 250 mg)에 대한 베아세프정의 생물학적 동등성

이윤석,강찬순,박은석,지상철 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

ABSTRACT-The bioequivalence of “Bearcef tablet”(Daewoong Pharmaceutical Co.), containing 250 mg cefuroxime (as cefuroxime axetil), in reference to“Zinnat tablet”(Glaxo Wellcome Korea Co.) was evaluated in 16 normal volunteers (age 21∼29 yrs) following the oral administration. After one tablet was administered, blood was taken at predetermined time intervals and the concentration of the drug in plasma was quantitated with an HPLC method. AUC, C_max and T_max were calculated and statistically analyzed for the bioequivalence of the two products. The results showed that the differences in AUC, C_max and T_max between two products were 6.92%, 3.49% and 5.26%, respectively. The powers for AUC, C_max and T_max were >90%, >90% and 89.0%, respectively. Confidence intervals for these parameters were all within ±20%. Judging based on the above results, “Bearcef tablet”is regarded to be bioequivalent to “Zinnat tablet”.

여성 복압성요실금에 대한 Tension-free Vaginal Tape(TVT) 수술 성적

문찬,강윤일,김경영,문형윤,노준,김철성 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Background: We wanted to evaluated the long term results of the tension-free vaginal tape (TVT) Procedure for treating strss urinary incontinence in women. Method: During Octover 1, 1998 to December 31, 2005, we evaluated the 52 women who underwent the TVT procedure for stress urinary incontinence at least 1 year following surgery. Preoperatively, Patients were evaluated with history taking, Physical examinations and urodynamic study, Operation and hospitalization data were evaluated with duration of procedure, hospital days, foley catheter removal days and use of analgesics, Postoperative evaluations included the uroflowmetry, residual urine volume, surgical outcomes, complications and patients' satisfaction with the procedure. Results: The follow-up period was a mean of 16 months. Preoperative I-QoL total score was 58.0. Comparision the result between preoperative and postoperative Q max was significantly shoter in the postoperative result. TVT procedure remained cure in 96.2% (cured: 82.7%, improved: 13.5%) and successful in 96.1%. Also, 49(94.2%) Patients would like to recommended the TVT procedure to others. There was no serious or long-term complications, Conclusions: The TVT procedure appear to be effective and safe for the surgical treatment of female stress urinary incontinence and showed a good long-term cure rate.

수종의 역충전 재료의 치근단 밀폐력 비교

황윤찬,강인철,황인남,오원만 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

This study was performed to evaluate the sealing ability of various retrograde filing materials by using bacterial penetration and dye penetration test. One hundred and forty extracted human teeth with single, straight canals and mature apices were collected and used for this study. All canals were instrumented using an engine driven Ni-Ti file (ProFile). After removing 3mm from the apex of tooth, a standardized 3mm root end cavity was prepared using an ultrasonic instrument. The 70 teeth were randomly divided into 7 groups : 6 groups for retrograde filling using Super-EBA, ZOE, Chelon-Silver, IRM, ZPC and amalgam. The 7th group was used as a negative control. Nail varnish was applied to all external root surfaces to the level of the resected root ends to prevent lateral microleakages. The specimens were then sterilized in an ethylene oxide sterilizer for 24 hours. 2 mm of the rejected root was immersed in a culture chamber containing a Tripticase Soy Broth with a phenol red indicator. The coronal access of each specimen was inoculated every 72 hours with suspension of Proteus vulgaris. The culture media were observed every 24hours for color change indicating bacterial contamination. The specimens were observed for 4weeks. The remaining 70 teeth were submitted to a dye penetration test. The canals of all teeth were first sealed with AH26 and obturated using an Obtura II system. Root resection, root end preparation and retrograde filling was performed as above. All specimens were suspended in 2% methylene blue dye for 72 hours before being longitudinally split. The degree of dye penetration was then measured using a stereomicroscope at 10 magnification and evaluated. The results were as follows : 1.In the bacterial penetration, the degree of leakage was the lowest in the Super-EBA, followed by, in ascending order, ZOE, Chelon-Silver IRM and ZPC. The amalgam showed highest bacterial leakage of all(p<0.01) . 2.In the dye penetration, the degree of microleakage was the lowest in the Chelon-Silver and Super-EBA, followed by, in ascending order, IRM, ZPC. The ZOE and amalgam showed the highest microleakage of all(p<0.05) . These results suggested that the eugenol based cement, Super-EBA, have excellent sealing ability as a retrograde filling material.

藥鍼用蜂毒液이 黑色腫細胞에 미치는 抗癌效果에 對한 分子生物學的 硏究

朴贊烈,南相水,金昌煥,李栽東,姜成吉,李潤浩,安秉哲 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidnine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1.The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2.Significant induction of apoptosis was identified using tryphan blue assay. [³H]thymidine release assay, and flow cytometric analysis of sub G₁fraction. 3.In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4.Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-X□ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5.In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of G₁phase was increased by a dose-dependant manner. 6.In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

백반증 병변에 국한되어 발생한 건선 1예

김윤전,강희영,이은소,김유찬 대한피부과학회 2006 대한피부과학회지 Vol.44 No.4

레진모형근관에서 엔진구동형 및 수동형 ProTaper 파일로 근관성형 후 근관형태 변화에 대한 비교연구

양인석,강인철,황윤찬,황인남,오원만 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.5

본 연구는 형태 및 재질이 동일한 엔진구동형 ProTaper 파일과 수동형 ProTaper 파일을 이용하여 레진모형근관을 성형한 후 근관형태 변화를 비교 분석하고자 시행되었다. 본 연구에서는 레진모형근관으로 총 40개의 J자와 S자의 근관형태가 재현된 ENDO-TRAINING BLOC을 사용하였다. 근관 성형 기구로는 엔진구동형 Ni-Ti 파일로 ProTaper^(TM), 수동형 Ni-Ti 파일로 ProTaper^(®) For Hand Use를 사용하였다. 사용된 레진모형근관과 파일의 종류에 따라 10개씩 4개의 그룹으로 나누어 근관성형을 시행하였다. 근관 성형 전 · 후 이미지를 스캐너 (Color scanner, UMAX Techologies, Inc., USA)를 이용하여 얻은 후, Photoshop 7.0 프로그램 (Adobe System Inc., USA)을 이용하여 이미지를 중첩하였다. 이미지 분석 프로그램 (Image-Pro^(®) Plus, Media Cybernetic, USA)을 이용하여 치근단 쪽에서부터 0, 1, 2, 3, 4, 5, 6 및 7 ㎜ 수준에서 근관 성형에 따른 근관의 내측 및 외측 폭경의 변화량, 근관 총폭경의 변화량, 근관 중심축의 변위량을 측정하였다. 또한 근관 성형 시간을 기록하였다. 두 기구 사이의 유의성 검정을 위해 독립 표본 t-검정을 시행하여, 근관 성형 시 수동형 ProTaper 파일에 엔진구동형 ProTaper 파일에 비해 근관 중심축의 변위가 유의하게 덜 일어나며 근관의 원래 형태를 더욱 잘 유지할 수 있지만, 근관 성형시간은 길어질 수 있다는 결론을 얻었다. The purpose of this study was to compare the canal configuration after shaping by ProTaper rotary files and ProTaper hand files in resin simulated canals. Forty resin simulated canals with a curvature of J-shape and S-shape were divided into four groups by 10 blocks each. Simulated root canals in resin block were prepared by ProTaper rotary files and ProTaper hand files using a crown-down pressureless technique. All simulated canals were prepared up to size #25 file at end-point of preparation. Pre- and post-instrumentation images were recorded with color scanner. Assessment of canal shape was completed with an image analysis program. Measurements were made at 0, 1, 2, 3, 4, 5, 6 and 7 ㎜ from the apex. At each level, outer canal width, inner canal width, total canal width, and amount of transportation from original axis were recorded. Instrumentation time was recorded. The data were analyzed statistically using independent t-test. The result was that ProTaper hand files cause significantly less canal transportation from original axis of canal body and maintain original canal configuration better than ProTaper rotary files, however ProTaper hand files take more shaping time.

수종의 근관세척액과 Listerine의 항균성 비교 연구

김영훈,강민경,최은경,양소영,양인석,강인철,황윤찬,황인남,오원만 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

본 연구는 여러 가지 근관세척액(NaOCl, CHX, EDTA)과 구강세정제로 사용되는 Listerine을 근관감염균주인 Porphyromonas gingivalis와 Enterococcus faecalis를 상대로 항균효과를 비교하고 Listerine이 근관세척액으로 사용가능한지를 확인하고자 시행하였다. 본 연구에서는 Porphyromonas gingivalis ATCC 3327과 Enterococcus faecalis ATCC 29212의 표준균주를 사용하였다. 실험을 위한 근관세척제로 0.1%, 0.2%, 1%, 2% Chlorhexidine(CHX)과 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.5M EDTA(18.6% EDTA). 그리고 Listerine원액을 이용하였다. 항균효과의 비교는 액체배지상에서 균주의 혼탁도와 한천 확산법을 이용한 억제대 비교로 하였다. 모든 실험군은 대조군과 비교시 근관내 균주에 항균성을 나타냈다(p < 0.001). 모든 농도의 NaOCl, CHX, 그리고 EDTA는 실험균주에서 높은 항균성을 보였다. 모든 실험에서 Listerine은 다른 근관세척제에 비해 낮은 항균성을 보였다. 결론적으로, Listerine이 E. faecalis와 P. gingivalis에 대해 항균성을 보이나, 일반적으로 사용되는 근관세척 액에 비해서는 현저히 낮은 항균성을 나타냄으로 근관세척 액으로 사용은 적합하지 않음을 나타냈다. The purpose of this study is to compare the antibacterial effect of Listerine on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using Listerine as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and Listerine were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of Listerine and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p < 0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, Listerine showed significantly low antibacterial effect compared with the other root canal irrigants (p < 0.05). In conclusion, the results reflect remarkably low antibacterial effect of Listerine as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

근관 전색재의 방사선 불투과성에 관한 비교연구

김태민,김서경,황인남,황윤찬,강병철,윤숙자,이재서,오원만 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.1

This study was performed to assess the radiopacity of a variety of root canal sealers according to the Specification concerning root canal sealers. Ten materials including Tubli-Seal™, Kerr Pulp Canal Sealer™, AH 26^(R), AH plus^(R), AH plus jet™, Ad seal™, Sealapex™, NOGENOU™, ZOB seal™, Epiphany™, and dentin were evaluated in this study. In the first part. densitometric reading of an each step of aluminum step wedge on occlusal film was performed at different voltage and exposure time. In the second part, ten specimens were radiographed simultaneously with an aluminum step wedges on the occlusal films under decided condition. The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained 1 Among the various conditions, the appropriate voltage and exposure time that meet the requirement density was 60 kVp at 0.2 s 2 All of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards. 3 The radiopacity of materials increased as thickness of materials increased. 4 The mm Al value of each specimen at 1mm in thickness has a significant difference in the statistics. It suggests that root canal sealers have a sufficient radiopacity that meet the requirement. 다양한 근관전색제는 방사선 사진상 주위 해부학적 구조와 구별될 만한 방사선 불투과성을 나타내야 한다 따라서 이런 물질들이 근관에 충전될 때의 방사선 불투과성 정도를 평가해야 할 필요성이 대두되고 있다. 본 실험에서는 다양한 근관전색제들의 방사선 불투과성을 평가하고자, 방사선 노출조건에 따른 aluminium step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 전색재의 방사선 불투과성 정도를 알루미늄 두께로 환산하여 비교해 보고자 한다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge을 사용하여, 60kVP, 70kVp관전압 상태에서 각각 0.2, 0.3, 0.4초 그리고 0.2, 0.3, 0.33초의 꼭 노출시간으로 교합필름상에서 방사선 촬영후 적절한 노출 조건을 구하였다. 직경 5mm 각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm인 10종 (Tubli-Seal™, Kerr pulp Canal Sealer™, AH26^(R), AHplus^(R), AH plus jet starter kit™, Ad seal™, Sealapex™, Nogenol root canal sealer™, ZOB seal™, Epiphany™)의 근관전색재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편, aluminum step wedge와 함께 정해진 노출시간에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 방사선 흑화도를 densitometer로 5회 반복 측정 후, 평균값을 구하여 알루미눔 두께로 환산하였다.얻어진 정보를 분석하여 다음의 결과를 얻었다. 1 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때 흑화도가 ISO No. 6876 규격에 가장 적합한 것은 60 kVP, 0.2초 일 경우였다. 2 측정된 근관 전색제의 방사선 불투과성은 2.29 mm Al (N0GEN0L)로부터 13.69 mm Al (AH Plus jet)까지 다양하게 나타났으나, 모두 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001) 규격이 제시한 최소한 3mm Al 이상의 방사선 불투과성을 지녀야 한다는 기준에 적합하였다. 3 재료의 두께가 증가할수록 방사선 불투과성은 증가하지만, 정비례하지는 않았다. 4 각 실험재료의 1 mm 두께의 시편에 대한 mm Al값들은 통계적으로 유의한 차이가 있었다. 이상의 결과는 본 실험에 사용된 수종의 근관 전색재는 모든 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.