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      • KCI등재

        Comparing eight types of ginsenosides in ginseng of different plant ages and regions using RRLC-Q-TOF MS/MS

        Yu-Lin Dai,Meng-Dan Qiao,Peng Yu,Fei Zheng,Hao Yue,Shu-Ying Liu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2

        Background: This article aims to compare and analyze the contents of ginsenosides in ginseng ofdifferent plant ages from different localities in China. Methods: In this study, 77 fresh ginseng samples aged 2e4 years were collected from 13 differentcultivation regions in China. The content of eight ginsenosides (Rg3, Rc, Rg1, Rf, Rb2, Rb1, Re, and Rd) wasdetermined using rapid resolution liquid chromatography coupled with quadrupoleetime-of-flighttandem mass spectrometry (RRLC-Q-TOF MS/MS) to comparatively evaluate the influences of cultivationregion and age. Results: Ginsenoside contents differed significantly depending on age and cultivation region. The contentsof ginsenosides Re, Rc, Rg1, Rg3, and Rf increased with cultivation age, whereas that of ginsenoside Rb1peaked in the third year of cultivation. Moreover, the highest ginsenoside content was obtained fromChangbai (19.36 mg/g) whereas the lowest content was obtained from Jidong (12.05 mg/g). Ginseng fromJilin Province contained greater total ginsenosides and was richer in ginsenoside Re than ginseng of thesame age group in Heilongjiang and Liaoning provinces, where Rb1 and Rg1 contents were relatively high. Conclusion: In this study, RRLC-Q-TOF MS/MS was used to analyze ginsenoside contents in 77 ginsengsamples aged 2e4 years from different cultivation regions. These patterns of variation in ginsenosidecontent, which depend on harvesting location and age, could be useful for interested parties to chooseginseng products according to their needs.

      • KCI등재

        Quantitative analysis of proteins related to chemoresistance to paclitaxel and carboplatin in human SiHa cervical cancer cells via iTRAQ

        Yue He,Su-Bin Han,Yu-Ning Geng,Shu-Li Yang,Yu-Mei Wu 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.3

        Objective: This study aimed to identify proteins related to paclitaxel and carboplatin chemoresistance in cervical cancer. Methods: Quantitative proteomic analysis was performed on normal SiHa cells and those treated with paclitaxel and carboplatin for 14 days, with isobaric tags for relative and absolute quantitation (iTRAQ) analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to identify related processes and differentially expressed proteins. Results: A total of 67 and 96 differentially expressed proteins were identified in the paclitaxel- and carboplatin- treated groups, respectively. GO and KEGG enrichment analyses identified 53 (43 upregulated and 10 downregulated) and 85 differentially expressed proteins (70 upregulated and 15 downregulated) in the paclitaxel- and carboplatin-treated groups, respectively. The cell counting kit-8 results revealed that APOA1 was overexpressed in both the paclitaxel- and carboplatin- resistant SiHa cells compared with the control cells. Immunohistochemistry showed that APOA1 was highly expressed in the paclitaxel- and carboplatin- resistant squamous cell carcinoma of the cervix. Conclusion: This study is the first to use iTRAQ to identify paclitaxel- and carboplatin- resistance proteins in cervical cells. We identified several proteins previously unassociated with paclitaxel and carboplatin resistance in cervical cancer, thereby expanding our understanding of paclitaxel and carboplatin resistance mechanisms. Moreover, these findings indicate that the APOA1 protein could serve as a potential marker for monitoring and predicting paclitaxel and carboplatin resistance levels.

      • SCIESCOPUSKCI등재

        Experimental studies of suppressing effectiveness on sloshing with two perforated floating plates

        Yu, Yue-Min,Ma, Ning,Fan, She-Ming,Gu, Xie-Chong The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.1

        In the present paper, model tests of suppressing sloshing fitted with two perforated floating plates are carried out. The study involves identification of system performance such as the suppression and the solidity ratio. Three different solidity ratios of perforated plates have been tried out as potential positive slosh damping devices. A series of painstaking experiments have been conducted in a rigid rectangular tank on six degrees of freedom motion platform under roll harmonic excitation. Comparison of the clean tank shows that the three types of perforated plates are all effective on damping the run-up and impact pressure along the bulkhead. The parametric study indicates that the perforated plate with the median solidity ratio is the most optimal one in suppressing sloshing among three configurations.

      • KCI등재

        More precise determination of V_cb & V_ub and direct CP violation in charmless B decays

        Yue-liang Wu,Yu-Feng Zhou,YWhen-Yu Wang 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.2

        More precise extractions on the two important CKM matrix elements jVcbj and jVubj are presented up to 1=m2 Q corrections, based on the complete heavy-quark eective eld theory (HQEFT) of QCD. The HQEFT as a large component QCD provides us with a complete theoretical framework for correctly and systematically evaluating the subleading and higher-order contributions in 1=mQ expansion of heavy quarks. A global analysis on charmless B decays is made, based on the isospin and SU(3) avor symmetry. An isospin relation is found to be very useful for studying SU(3) symmetry-breaking eects of strong phases and exploring new types of electroweak penguin eects. The direct CP violation in charmless B decays is predicted, and its precise measurement is helpful either for testing SU(3) symmetry breaking eects of strong phases or for probing new physics beyond standard model.

      • Knockdown of Cdc25B in Renal Cell Carcinoma is Associated with Decreased Malignant Features

        Yu, Xiu-Yue,Zhang, Zhe,Zhang, Guo-Jun,Guo, Kun-Feng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

        Cdc25 phosphatases are important regulators of the cell cycle. Their abnormal expression detected in a number of tumors implies that their dysregulation is involved in malignant transformation. However, the role of Cdc25B in renal cell carcinomas remains unknown. To shed light on influence on renal cell carcinogenesis and subsequent progression, Cdc25B expression was examined by real-time RT-PCR and western blotting in renal cell carcinoma and normal tissues. 65 kDa Cdc25B expression was higher in carcinomas than in the adjacent normal tissues (P<0.05), positive correlations being noted with clinical stage and histopathologic grade (P<0.05). To additionally investigate the role of Cdc25B alteration in the development of renal cell carcinoma, Cdc25B siRNA was used to knockdown the expression of Cdc25B. Down-regulation resulted in slower growth, more G2/M cells, weaker capacity for migration and invasion, and induction of apoptosis in 769-P transfectants. Reduction of 14-3-3 protein expression appeared related to Cdc25B knockdown. These findings suggest an important role of Cdc25B in renal cell carcinoma development and provide a rationale for investigation of Cdc2B-based gene therapy.

      • KCI등재

        Reasoning and fuzzy comprehensive assessment methods based CAD system for boiler intelligent design

        Yue-xi Yu,Hong-kai Liao,Yi Zhou,Wei Zhong 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.3

        Boilers are key industrial equipment in the field of modern manufacturing. In this study, rule-based reasoning (RBR) and case-basedreasoning (CBR) are applied into the boiler intelligent design. RBR is adopted to perform like a “sieve” for the acquisition of candidatestructure modules. Then, fuzzy comprehensive assessment method is used to select an optimal structure module among the candidates. The optimal structure module is subjected to the CBR process, which includes modification and case retention. It is a minor adjustmentprocess that needs a great amount of prior knowledge. The case retention process gives the system a self-enrich function, which will improvethe design ability of the system with continuous use of the system. A case study is presented to validate the correctness and efficiencyof proposed method.

      • Research on the Resource Scheduling of the Improved SFLA in Cloud Computing

        Yue Miao,Fu Rao,Luo Yu 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1

        It has always been a key topic in the current research how to make rational resource scheduling in the cloud computing environment. In this paper, the status of cloud computing resources is first analyzed, to point out the existing problems, and then combined with the characteristics of resource scheduling in cloud computing, the Shuffled Frog Leaping Algorithm is introduced. First, in its stage of subgroups classification, the chaos strategy is introduced and in the internal search the positive learning strategy is introduced, which makes the improved frog leaping algorithm gain good convergence, reduces the time of global search and optimization. Through the CloudSim platform, it shows that this algorithm can improve the efficiency of task processing and make the resource scheduling in cloud computing rational and effective.

      • Alveolar bone thickness around maxillary central incisors of different inclination assessed with cone-beam computed tomography

        Yu-lou Tian,Fang Liu,Hong-jing Sun,Pin Lv,Yu-ming Cao,Mo Yu,Yang Yue 대한치과교정학회 2015 대한치과교정학회지 Vol.45 No.5

        Objective: To assess the labial and lingual alveolar bone thickness in adults with maxillary central incisors of different inclination by cone-beam computed tomography (CBCT). Methods: Ninety maxillary central incisors from 45 patients were divided into three groups based on the maxillary central incisors to palatal plane angle; lingual-inclined, normal, and labial-inclined. Reformatted CBCT images were used to measure the labial and lingual alveolar bone thickness (ABT) at intervals corresponding to every 1/10 of the root length. The sum of labial ABT and lingual ABT at the level of the root apex was used to calculate the total ABT (TABT). The number of teeth exhibiting alveolar fenestration and dehiscence in each group was also tallied. One-way analysis of variance and Tukey’s honestly significant difference test were applied for statistical analysis. Results: The labial ABT and TABT values at the root apex in the lingual-inclined group were significantly lower than in the other groups (p < 0.05). Lingual and labial ABT values were very low at the cervical level in the lingual-inclined and normal groups. There was a higher prevalence of alveolar fenestration in the lingual-inclined group. Conclusions: Lingual-inclined maxillary central incisors have less bone support at the level of the root apex and a greater frequency of alveolar bone defects than normal maxillary central incisors. The bone plate at the marginal level is also very thin.

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