소의 시상하부내에서 Vasopressin과 Oxytion분비세포의 분포에 관한 면역조직화학적 연구

유명철,김종중,장인엽,문정석,정주현,김흥중,조사선 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was done to observe the distributions of Oxytocinergic and Vasopressinergic neurons in the hypothalamus of the cow. For the immunohistochemical staining, sections were reacted with the following sequence of solutions: monoclonal anti-vasopressin and anti-oxytocin, biotinylated anti-mouse IgG, avidin-biotin peroxidase complex, and Ni-DAB. The results observed under light microscope were summarized as follows. Our immunohistochemical investigations reveal both vasopressinergic and oxytocinergic neurons in the supraoptic nucleus(SON), paraventricular nucleus(PVN), accessory supraoptic nucleus (ASN), periventricular nucleus(PN), suprachiasmatic nucleus(SCN), and arcuate nucleus(AN), but these two types of neurons were mainly distributed in the PVN and SON and the ratio of the number of vasopressinergic neurons to that of oxytocinergic neurons was 1:1.30 in the PVN, and 1:1.00 in the SON. These two types of neurons were round, oval and spindle-shaped and cell size was 30-55um.

컬럼형 회분건조기를 이용한 벼의 건조에 관한 연구

변유량,최홍식,조형용,권윤중,김주봉 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.1

The vertical column batch dryer which is suitable to Korean farm was constructed and dryer performance was investigated through field test. The effect of a drying air temperature and an airflow rate on the drying rate was studied, and an empirical drying equation was established. The average drying rate of paddy were 1.6-1.8%/hr at the air temperature of 43℃ and the airflow rates of 19.4 - 24.5 CFM/ft². The differences of grain moisture content between the inner and outer layer of vertical paddy bed were 0.5 - 0.9% The effect of a drying air temperature was larger at high airflow rate. At constant temperature, the drying rate was increased as an airflow rate was increased. The empirical drying equation could be expressed as followed: ??=0.9exp(??) Where K=3.22×?? The average whitening ratio of brown rice which was dried with column batch dryer was higher 0.6% than that of sun dried rice.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

한국인 환자에서 Vancomycin의 집단약동학 (Population Pharmacokinetics)에 관한 연구

유경상,배균섭,이주연,조주연,장인진,신상구,신완균 大韓臨床藥理學會 1998 臨床藥理學會誌 Vol.6 No.2

좌측흉수로 발현된 Boerhaave 증후군 1예

이준,변유미,조민근,조주연,원경준,김영대,박찬국,김만우,유재근,서홍주,이석기 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

구토 후 발생한 토혈을 주소로 내원한 환자에서 좌측 늑막 삼출 동반 시 식도 파열의 가능성을 고려하여 흉수 천자 및 지속적인 흉부 단순 촬영의 추적관찰이 필요하며 식도 파열이 배제된 후 환자의 생체 징후 및 토혈 양을 파악하여 내시경 검사 시기를 신중히 결정하는 것이 좋을 것으로 생각된다. 본 증례는 토혈을 주소로 내원하여 Mallory-Weiss 증후군 의심 하에 내시경을 통한 치료 시행 후 추적 관찰에 의해 진단된 Boerhaave 증후군을 경험하였기에 보고하는 바이다. Boerhaave's syndrome is a spontaneous perforation of the esophagus most commonly resulting from a sudden increase in intraesophageal pressure combined with negative intrathoracic pressure caused by straining or vomiting. Surgery should generally be performed within 24 hours because mortality approached 30 to 50 percent in which surgery was delayed. Therefore, early diagnosis and prompt surgical repair are critical for survival. We have experienced a case of Boerhaave's syndrome in a patient presenting with hematemesis with left pleural effusion without any signs or symptoms of pneumomediastinum or pneumothorax.

글루코오스와 Chloramphenicol이 알카리내성 Bacillus속 YA-14 Promoter의 발현에 미치는 영향

김인규,이석훈,조형용,변유량,유주현 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.2

알칼리내성 Bacillus sp.의 chromosomal DNA로부터 분리하여 공여균주에 삽입된 promoter의 발현 특성에 미치는 글루코오스와 chloramphenicol 농도의 영향을 CAT 활성을 측정하여 연구하였다. Promoter는 초기 글루코오스 농도에 상관없이 대수 증식기가 끝난 이후, 글루코오스가 저농도로 감소되어 catabolic repression이 해제된 시점부터 활성이 급격히 증가하여 포자형성 초기단계에 최대 활성을 나타내고 포자가 형성됨에 따라 감소하였다. 재조합된 plasmid는 접종 후 40시간이 경과한 후까지도 95% 이상의 높은 안정성을 유지하였다. 초기 글루코오스의 농도 및 chloramphenicol의 첨가량과 첨가시기는 CAT 활성에 큰 영향을 미쳐 초기 글루코오스의 농도는 1g/l, chloramphenicol은 초기부터 농도 10㎍/㎖로 첨가하여 배양했을 때 가장 높은 활성을 얻었다. This promoter from alkali-tolerant Bacillus sp. YA-14 chromosomal DNA is unexpressed in vegetatively growing cells under excess glucose. After glucose in the medium consumed nearly completely, the activity of the inserted promoter was dramatically increased, and its maximum activity was attained in the initial stage of sporulation. The plasmid cloned in Bacillus sp. YA-14 was stable for 40 hours. The optimal initial concedntrations of glucose and chloramphenicol were 1 g/l and 10 ㎍/㎖, respectively for the maximum expression of the promoter.

간경변 환자에서 Oro-cecal Transit Time의 측정

원경준,이준,변유미,조민근,조주연,서정균,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Neurotransmitter like substances in the gut have been proposed to contribute to the overall portal systemic encephalopathy (PSE). The source of the ammonia in patients with PSE is mainly the small bowel, as a result of the conversion of glutamine in the diet to glutamate and ammonia. PSE is highly responsive to changes in diet, to antibiotic therapy and to ingestion of nondigestible disaccharides such as lactulose. This study was performed to evaluate the relationship between PSE and oro-cecal transit time. In addition, performed to evaluate the effect of severity, cause, complications of liver cirrhosis on oro-cecal transit time. Methods: Between August 2006 and February 2007, 48 cirrhotic patients consecutively was enrolled in study their oro-cecal transit time after informed consent was obtained. A non-invasive hydrogen breath test was used to study oro-cecal transit time (OCTT). Result: The OCTT were delayed in cirrhotic patients with PSE compared without PSE (123.3 ± 42.7 min vs 170.0 ± 58.3 min, p<0.05). OCTT tended to prolongate further as the damage in the liver got worse, nevertheless the result was statistically meaningless (p<0.111). Neither presence of ascites or cause of PSE had any influence on gastrointestinal transit. Conclusions: Cirrhotic patients with PSE have more delayed OCTT than without PSE. Further research is needed to find out the relationship between OCTT and severity of liver cirrhosis.

Modified AS1411 Aptamer Suppresses Hepatocellular Carcinoma by Up-regulating Galectin-14

( Hyeki Cho ),( Yun Bin Lee ),( Yuri Cho ),( Jeong-hoon Lee ),( Dong Hyeon Lee ),( Jeong-ju Yoo ),( Young Youn Cho ),( Eun Ju Cho ),( Su Jong Yu ),( Yoon Jun Kim ),( Jong In Kim ),( Jong Hun Im ),( Ju 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Aptamers are small synthetic oligonucleotides that bind to target proteins with high specificity and affinity. AS1411 is an aptamer that binds to the protein nucleolin, which is overexpressed in the cytoplasm and occurs on the surface of cancer cells. We investigated the therapeutic potential of aptamers in treating hepatocellular carcinoma (HCC) by evaluating anti-tumor effects and confirming the affinity and specificity of AS1411 and modified AS1411 aptamers in HCC cells. Methods: Cell growth was assessed using the MTS assay, and cell death signaling was explored by immunoblot analysis. Fluore- scence-activated cell sorting was performed to evaluate the affinity and specificity of AS1411 aptamers in SNU-761 HCC cells. We investigated the in vivo effects of the AS1411 aptamer using BALB/c nude mice in a subcutaneous xenograft model with SNU-761 cells. Results: Treatment with a modified AS1411 aptamer significantly decreased in vitro (under normoxic [P=0.035] and hypoxic [P=0.018] conditions) and in vivo (under normoxic conditions, P=0.041) HCC cell proliferation compared to control aptamers. AS1411 and control aptamers failed to control HCC cell proliferation. However, AS1411 and the modified AS1411 aptamer did not induce caspase activation. Decrease in cell growth by AS1411 or modified AS1411 was not prevented by caspase or necrosis inhibitors. In a microarray, AS1411 significantly enhanced galectin-14 expression. Suppression of HCC cell proliferation by the modified AS1411 aptamer was attenuated by galectin-14 siRNA transfection. Conclusions: The modified AS1411 aptamer suppressed HCC cell growth in vitro and in vivo by up-regulating galectin-14 expression. Modified AS1411 aptamers may have therapeutic potential as a novel targeted therapy for HCC.

A novel prognostic factor for hepatocellular carcinoma: protein disulfide isomerase

Yu, Su Jong,Won, Jae-Kyung,Ryu, Han Suk,Choi, Won-Mook,Cho, Hyeki,Cho, Eun-Ju,Lee, Jeong-Hoon,Kim, Yoon Jun,Suh, Kyung-Suk,Jang, Ja-June,Kim, Chung Yong,Lee, Hyo-Suk,Yoon, Jung-Hwan,Cho, Kwang-Hyun The Korean Association of Internal Medicine 2014 The Korean Journal of Internal Medicine Vol.29 No.5

<P><B>Background/Aims</B></P><P>Protein disulfide isomerase (PDI) has been implicated in the survival and progression of some cancer cells, by compensating for endoplasmic reticulum stress by upregulating the protein-folding capacity. However, its prognostic role in patients with hepatocellular carcinoma (HCC) has not been investigated.</P><P><B>Methods</B></P><P>We collected HCC tissues from 83 HCC patients who underwent surgical resection for an immunohistochemical study of PDI. Overall survival (OS) was measured from the date of surgical resection until the date of death from any cause. Radiological progression was evaluated using the modified Response Evaluation Criteria in Solid Tumors in an independent radiological assessment.</P><P><B>Results</B></P><P>PDI expression was found to be increased in human HCC compared to adjacent nontumor tissues. Increased immunopositivity for PDI was associated with a high Edmondson-Steiner grade (<I>p</I> = 0.028). Univariate analysis of patients who had undergone surgical resection for HCC showed that tumor PDI upregulation is a significant risk factor for poor OS (<I>p</I> = 0.016; hazard ratio [HR], 1.980) and time to progression (TTP; <I>p</I> = 0.007; HR, 1.971). Multivariate analyses revealed that high PDI expression was an independent predictor of a shorter TTP (<I>p</I> = 0.015; HR, 1.865) and poor OS (<I>p</I> = 0.012; HR, 2.069).</P><P><B>Conclusions</B></P><P>Upregulated PDI expression is associated with aggressive clinicopathological features of HCC; thus, PDI might serve as an independent prognostic factor and a potential therapeutic target for HCC patients.</P>

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>