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류영욱,이규석 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3
The expression of elastin gene is examined by skin fibroblast cultures and placental tissues using Northern and slot-blot hybridization. Total RNA from 3-6 passaged fibroblasts cultures was isolated. In Northern blot analysis, elastin, fibronectin and β-actin mRNA transcripts from cultured keloid and normal skin fibroblast and placenta, demonstrated the specificity of hybridization with elastin, frbronectin and β-actinl. Elastin cDNA prlbe hybridized mRNA with sizes of 3.5kb. The molecular size of fibronectin and β-actin mRNA revealed 8.0kb and 2.0kb, respectively. Therefore, it showed no difference of size of mRNAs among keloid and normal skin fibroblst and placenta tissues. In slot blot analysis, the levels of elastin in keloid is higher 1.46 fold than control skin fibroblast. And determination of elastin/type I collagen ratio revealed 0.067 in control skin fibroblast and 0.031 in keloid. This results indicate that elastin gene expression in keloid is increased compared to control skin fibroblst and placenta.
피부종양에서 Single-strand Conformation Polymorphisms 법에 의한 p53 유전자 돌연변이 검색
류영욱,손보성,권호준,이규석,송준영 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4
The tumor suppressor gene p53, located on the short arm of chromosome 17, encodes for nuclear protein which regulates cell proliferation by inhibiting cells entering S-phase. Mutation in p53 gene are the most frequent genetic alteration found in human cancers to date. This study was examined mutant p53 gene mutation using PCR-SSCP method and mutant p53 oncoprotein expression using avidin biotin peroxidase complex method immunohistochemically, in 2 seborrheic keratosis, 3 basal cell carcinoma, 3 squamous cell carcinoma and 1 metaststic squamous cell carcinoma cases. With PCR-SSCP methods in exon 5 and 7, no genetic mutation of p53 gene was observed in 2 seborrheic keratosis, 3 basal cell carcinoma, 3 squamous cell carcinoma, and 1 metaststic squamous cell carcinoma cases. With immunohistochemical staining with ABC method, a case of squamous cell carcinoma developed on lower lip was positive but another were all negative. This result means that p53 gene mutation on skin tumor may develope in squamous cell carcinoma developed on sun exposed area and this mutation on squamous cell carcinoma can develope other exonal site except exon 5 and 7.
최윤애,류영욱,정재봉,송준영,이규석 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3
We experienced 3 cases of fixed type cutaneous sporotrichosis confined to the face. Case 1 was a 32-month old child, affected over the nose. Case 2 was a 25-year old man, affected over the left cheek. Case 3 was a 71-year old man, affected over the nose. Histopathologically, chronic granulomatous inflammation with pseudoepitheliomatous hyperplasia was observed. Mycologically, typical dark brown to black colored colonines with moist, wrinkled and membranous surfaces were cultured. All patients were completely cured by oral administration of saturated KI and itraconazole combined therapy in a short period(40-90 days).
손보성,송준영,정재봉,김병천,이상숙,류영욱,이규석 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4
Papulonecrotic tuberculid is an eruption of necrotizing papules, particularly affecting the extremities and occurring in more or less symmetrical crops, and responding to antituberculosis therapy. Individual lesions heal with scarring. Histologically, vascular involvement is seen in early lesions. Late lesions are divided into two forms. In superficial forms, wedge shaped area of necrosis forms in epidermis and upper dermis. Epithelial and lymphoid cells gather around its periphery. In deep forms, with no epidermal change, granulomatous changes surrounded by well defined palisading histiocyte and lymphoid, epithelioid cells are seen. In mid-dermis and perivascular area, vessel shows vasculitis. In the first case, leukocytoclastic vasculitis and early granuloma formation were seen. In the second case, palisading granuloma formation was seen. In the third case, tuberculoid granuloma formation was seen. We report three cases of papulonecrotic tuberculid showing three different stages of histological finding, in individual cases.
Ryoo, Young Wook,Lee, Kyu Suk,Choi, Dong Won 대한피부과학회 1999 Annals of Dermatology Vol.11 No.3
Background : bFGF, a member of the fibroblast growth factor family, potently induces vascular smooth muscle cell proliferation and decreased synthesis of the collagens. Objective : For further investigation of the effect of bFGF on extracellular matrix homeostasis in the skin, we evaluated the expression of type I and type VII collagen gene at the transcriptional levels. Method : We examined that recombinant human bFGF affects the expression of genes involved in ECM synthesis and remodeling in human dermal fibroblasts cultures as judged by Northern blot analysis. Results : The steady state levels of type I and VII collagen gene mRNA were decreased with age dependent pattern up to 0.13 and 0.44 folds respectively. The transcriptional levels of type I collagen mRNA were increased by TGF-ß, treatment but markedly decreased by bFGF as well as TNF-α. But there were no synergistic effects bFGF and TNF-α on type I collagen gene expression. The levels of type VII collagen gene expression were increased by both bFGF and TGF-ß,. The TNF-α showed slightly antagnastic effects on type VII collagen gene expression. Conclusion: The type I and VII collagen gene expression in dermal fibroblasts is clearly subjected to modulation by the cytokines including bFGF with uncoordinate regulatory pathway. In addition to its function of vascular proliferation, bFGF also may play a major role in physiologic skin condition and in repair process such as formation of a stable dermoepidermal junction during skin wound healing.
Primary Cutaneous Cryptococcosis Successfully Treated With Fluconazole
Ryoo, Young Wook,Lee, Kyu Suk,Park, Jun Hyoung 대한피부과학회 2000 Annals of Dermatology Vol.12 No.2
We report a case of primary cutaneous cryptococcosis on Rt. forehead and perioral area of 57 year old woman with non-insulin dependent diabetes mellitus and Lt. cerebral infarction. She had large ulcers with yellowish purulent exudates on Rt. forehead and perioral area for 2months. A histopathological examination from the lesion showed numerous encapsulated, round spores and the organisms were identified as Cryptococcus neoformans in a series of fungal studies. The patient received a 5-week course of IV and oral fluconazole with resolution of her skin lesion. The patient is free of any lesion several months after completing therapy. This experience supports the use of flucanazole as initial and single therapy in primary cutaneous cryptococcosis.
Porokeratosis ptychotropica: a case report
Young-Wook Ryoo,Yura Kim,Ji-Min Yun,Sung-Ae Kim 영남대학교 의과대학 2023 Yeungnam University Journal of Medicine Vol.40 No.4
Porokeratosis ptychotropica is an uncommon form of porokeratosis, which was initially described in 1995. It is clinically characterized by symmetrical reddish to brown-colored hyperkeratotic, verrucous, or psoriasiform plaques on the perianal and gluteal regions. The lesions tend to integrate and expand centrally, with small peripheral satellite lesions. Early skin biopsy and appropriate diagnosis are essential because malignant change occurs in 7.5% of porokeratotic lesions. Conventional treatment options include topical steroid, retinoid, imiquimod, 5-fluorouracil, isotretinoin, excimer laser, photodynamic therapy, intralesional steroid or bleomycin injection, cryotherapy, carbon dioxide (CO2) laser, and dermatome and excision, but none seem to achieve complete clearance. A 68-year-old woman presented with diffuse hyperkeratotic scaly lichenoid plaques on the buttocks that had persisted for several years. A skin biopsy of the buttocks revealed multiple cornoid lamellae and intense hyperkeratosis. There were some dyskeratotic cells beneath the cornoid lamellae and the granular layer was absent. Porokeratosis ptychotropica was diagnosed based on the characteristic clinical appearance and typical histopathological manifestations. She was treated with a CO2 laser in one session and topical application of urea and imiquimod cream for 1 month. The lesions slightly improved at the 1-month follow-up. We herein present a rare case of porokeratosis ptychotropica.