cDNA SEQENCES OF PHOSPHOLIPASE C - γ1 OF NORMAL AND MALIGNANT TUMOR TISSUES

Yee Sook Cho,Chung Ung Park,Uh Hyun Kim 생화학분자생물학회 1993 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

갈색세포종(증례보고)

조원현,이상숙,장은숙,박영관,예석형 啓明大學校 醫科大學 1998 계명의대학술지 Vol.7 No.1

Pheochromocytoma is a tumor which produces catecholamine and its metabolites and are usually benign unilateral neoplasm of adrenal medulla in more than 80%. In rare cases, it may arise in an extraadrenal sites including perirenal fat, paraganglia, urinary bladder, chest and neck. In less than 1% of hypertension population, pheochromocytoma is a cause of hypertension and most of these cases are curative by surgery. The most common clinical finding is hypertension and it has three frequent symptoms of headache, palpitation and perspiration. The tumor can be diagnosed by urinary excretion of catecholamine and their metabolites such as vanillylmandelic acid and metanephrines. Other tumor, compeuterlized tomogram, 1131 MIBG scitigram, selective arteriogram and selective caval sampling for catecholamine are used. Authors experienced an extraadreanl pheochromocytoma in 71 years old male with intermittent hypertension and huge abdominal mass so reported the case and reviewed literature.

Reliability and Validity of the Korean Version of the Infant-Toddler Social and Emotional Assessment

Kyung Sook Lee,Jinah Park,Goen Ho Bahn,Young Il Cho,Yee Jin Shin 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.5

Objective This article examined the psychometric properties of the Korean version of the Infant-Toddler Social and Emotional Assessment(K-ITSEA). Methods Translation and back-translation of the K-ITSEA were conducted after obtaining a permission. Two thousand two hundred thirty six Korean community infants (1,199 boys and 1,037 girls) between the ages of 12 and 36 months (M=34.23, SD=3.80) and 90 clinical infant samples (60 boys and 30 girls) between the ages of 12 and 36 months (M=26.84, SD=6.24) participated in the present study. Results Confirmatory factor analyses supported the Internalizing, Externalizing, Dysregulation, and Competence domains as well as the 17 individual scales that comprise the K-ITSEA. Young children’s sex and age differences emerged for some problem and most competence scales. All domains showed adequate intrascale reliability and test-retest reliability. Scale intracorrelation analyses and associations between the K-ITSEA and Korean version of PSI, Korean version of CBCL1.5-5 supported the validity of the assessment. Comparisons of the K-ITSEA scores for the Autism Spectrum Disorder, Psychiatric Disorders and Matched control groups supported the discriminant validity of the K-ITSEA. Conclusion This preliminary results indicate that the K-ITSEA would be a useful assessment for detecting the early childhood’s behavior problems and competences in Korean population.

신생아에 심한 심부전증을 일으킨 선천성 뇌동정맥 기형: 풍선 색전 요법을 시도한 1례

박인숙,김영휘,홍창의,조영국,서대철,박영일 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.2

Cerebral arteriovenous malformation is a rare cause of congestive heart failure in neonates and is known to carry a very poor prognosis even wish prompt recognition and aggressive treatment. We recently encountered a 2-day-old male neonate with huge cerebral arteriovenous malformation who presented with intractable heart failure. Despite aggressive medical treatment and partial embolization with detachable balloon, baby died of severe hearts failure. Since there is no report of percutaneous balloon embolization in a neonate with this lesion in Korean literature, we wish to present our experience in one such neonate. Also we believe this is the first report of color Doppler findings in cerebral arteriovenous malformation in Korean literature.

Vocalization of Emotional and Social Expressions in Korean-Speaking Toddlers with Autism Spectrum Disorder and Those with Developmental Delay

Lee, Kyung-Sook,Shin, Yee Jin,Yoo, Hee-Jeong,Lee, Gui Jong,Ryu, Jeong,Son, Oweol,Cho, Sook Whan Yonsei University College of Medicine 2018 Yonsei medical journal Vol.59 No.3

<P><B>Purpose</B></P><P>This study aimed to examine the development of socializing and emotional expressions through vocalizations and joint attention (JA) behaviors in Korean-speaking children with autism spectrum disorder (ASD), compared to those with developmental delay (DD).</P><P><B>Materials and Methods</B></P><P>Video samples were collected from 28 toddlers with ASD and 18 age-matched toddlers with DD, and vocalizations were each coded in detail for the purpose of this retrospective research. In addition to some statistical analysis, Computerized Language Analysis was conducted to obtain the final results.</P><P><B>Results</B></P><P>Although they produced a higher number of vocalizations than the DD group, the ASD group did not engage in emotional or social interactions with their caretakers, whereas the DD group did. The children with ASD used more atypical vocalizations and socially unengaged vocalizations than the children with DD did. JA using vocalizations in the ASD group, in particular, was largely dyadic, with triadic types occurring at a significantly lower frequency than those in the DD group.</P><P><B>Conclusion</B></P><P>Results from this study indicate the importance of assessing early vocalizations in toddlers with ASD, suggesting that some common symptoms of ASD, such as lack of typical, emotional, and social functions in early vocalizations, could be used to develop screening and intervention programs related to ASD.</P>

약물과민증 예방을 위한 처방전달 시스템 전산 프로그램의 이용

김윤이 ( Youn Yee Kim ),조유숙 ( You Sook Cho ),김태범 ( Tae Bum Kim ),이태훈 ( Tae Hoon Lee ),이재천 ( Jae Chun Lee ),고수진 ( Su Jin Koh ),박수길 ( Su Kil Park ),이순교 ( Sun Gyo Lee ),김철규 ( Chul Gyu Kim ),유빈 ( Bin Yoo ),문 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.1

A study on boron removal for seawater desalination using the combination process of mineral cluster and RO membrane system

Bong Yeon Cho,Hye Won Kim,Yee Sook Shin 대한환경공학회 2015 Environmental Engineering Research Vol.20 No.3

Complicated and expensive seawater desalination technology is a big challenge in boron removal process. Conventional seawater desalination process of coagulation utilized for pre-treatment is difficult to remove boron. Boron can be removed more effectively in Reverse Osmosis (RO) process than any other processes. In this study, a coagulant with the name Mineral Cluster was examined its boron removal ability. Boron removal efficiency of Mineral Cluster depended on pH value and Mineral Cluster dosage. Desalination process combines the pre-treatment process with Mineral cluster diluted at the ratio of 1:2500 and the RO membrane process. The original sea water could be desalinated to drinking water quality, 1 mg/L, without any pH adjustments. Therefore, if the Mineral cluster is added without any other chemicals for adjusting pH, the desalination process would be much safer, efficient and economical.

Effects of Inverted Incubation on Adventitious Root Formation in Epicotyl Cuttings of Vigna angularis Owhiet Ohashi

Kang, Byung-Sook,Cho, Duck-Yee,Kim, Young-Soon,Soh, Woong-Young The Korean Society of Plant Biotechnology 1998 식물생명공학회지 Vol.25 No.2

부정근발생에 관한 도립 및 직립배양의 영향을 구명하기 위하여 6일간 자란 팥의 유경 절편을 사용하였다. $2\times10$^{-4}M$ IAA 용액을 48시간 처리할 때에 절편의 위치를 직립 또는 도립으로 배양 후 96시간 직립으로 배양하였다. 48시간 처리시 직립배양하면 부정근의 형성은 형태학적 기부에서만 발생하지만, 도립 전처리 배양을 하면 유경절편의 양끝에서 부정근이 발생하였다. IAA 전처리는 절편의 방향과 관계없이 부정근 형성을 촉진시켰다. 부정근 발생시기에 유경절편내의 생화학적 변화를 구명하기 위하여 직립배양 및 도립배양을 한 후 부정근 발생 단계별로 24시간, 48시간, 148시간 후 절편을 상부, 중부, 하부로 등분하여 부정근 발생에 수반되는 효소활성, 절편의 길이 신장 및 생중량을 측정하였다. 절편의 길이 신장은 절편의 상부에서 높게 나타났고 생중량은 발근부위에서 높게 나타났다. peroxidase을 catalase 활성은 부정근원기의 형성단계인 24시간에 절편의 발근부위에서 높게 나타났고 이에 비해 IAA oxidase는 양쪽 기부에서 낮았다. 그리고 IAA oxidase와 peroxidase 활성은 48시간 배양인 부정근의 형성동안 발근 부위에서 감소하였다. 도립전처리 유경 절편에 따라서 효소활성의 변화가 일어났으며 peroxidase의 활성은 부정근원기형성과 밀접한 관계를 나타내었다. 따라서 생화학적인 변화는 유경의 발근영역에서 IAA의 수준의 변화와 밀접하게 관련되어 특정부위에서의 부정근 형성을 유도하는 것으로 판단된 것이다. Disbudded epicotyl cuttings from light-grown 6-day-old seedings of Vigna angularis Owhi et Ohashi were preincubated in $2\;\times\;10^{-4}M$ IAA solution for 48 hr to promote adventitious root formation in upright or inverted direction and then incubated in upright direction for 96 hr. Adventitious root formation occurred only at the morphological base of the cuttings which were preincubated in upright direction, while at the both ends in inverted direction. IAA treatment enhanced the adventitious root formation in all cuttings regardless of their orientation during preincubation. To elucidate localized root development, the activity of enzymes involved in root initiation and development was measured 24 hr, 48 hr, and 148 hr after epicotyl incubation. IAA oxidase, peroxidase and catalase were assayed in the apical, middle and basal segment of the epicotyls, and their fresh weight and length were measured. Elongation occurred the most in the upper segment of the epicotyl while fresh weight gain was the most in the basal segment. At root initiation phase, 24 hr after incubation IAA peroxidase and catalase activities appeared high at rooting zone while IAA oxidase activity was low at both ends, IAA oxidase and peroxidase activities declined at the rooting zone during the adventitious root formation at 48 ht. Inversion of cuttings during preincubation caused a chrange of enzyme activities along their epicotyl cuttings. Only peroxidase activity showed a high correlation with root initiation. Therefore, the biochemical change is highly correlated with change in IAA level in the rooting zone of the epicotyl, resulting in root formation in unusual rooting zone of epicotyl.

Pharmacological Regulation of Oxidative Stress in Stem Cells

Lee, Jungwoon,Cho, Yee Sook,Jung, Haiyoung,Choi, Inpyo Hindawi 2018 Oxidative medicine and cellular longevity Vol.2018 No.-

<P>Oxidative stress results from an imbalance between reactive oxygen species (ROS) production and antioxidant defense mechanisms. The regulation of stem cell self-renewal and differentiation is crucial for early development and tissue homeostasis. Recent reports have suggested that the balance between self-renewal and differentiation is regulated by the cellular oxidation-reduction (redox) state; therefore, the study of ROS regulation in regenerative medicine has emerged to develop protocols for regulating appropriate stem cell differentiation and maintenance for clinical applications. In this review, we introduce the defined roles of oxidative stress in pluripotent stem cells (PSCs) and hematopoietic stem cells (HSCs) and discuss the potential applications of pharmacological approaches for regulating oxidative stress in regenerative medicine.</P>

Simvastatin Suppresses Self-Renewal of Mouse Embryonic Stem Cells by Inhibiting RhoA Geranylgeranylation

Lee, Mi-Hee,Cho, Yee Sook,Han, Yong-Mahn Wiley (John WileySons) 2007 Stem Cells Vol.25 No.7

<P>Statins, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, were originally developed to lower cholesterol. Their pleiotropic (or cholesterol-independent) effects at the cellular and molecular levels are highly related to numerous cellular functions, such as proliferation and differentiation. However, they are hardly studied in embryonic stem cells. In this study, we evaluated the effects of statins on mouse ESCs (J1, D3, and RW.4) to enhance our understanding of the molecular basis of ESC self-renewal. Treatment of ESCs with simvastatin, mevastatin, atorvastatin, or pravastatin induced morphological change and decreased cell proliferation. We observed that the use of simvastatin was most effective in all three ESCs. Loss of ESC self-renewal by simvastatin was determined by marked downregulation of ESC markers alkaline phosphatase, Oct4, Nanog, Rex-1, and SSEA-1. Simvastatin effects were selectively reversed by either mevalonate or its metabolite geranylgeranyl pyrophosphate (GGPP) but not by cholesterol or farnesyl pyrophosphate. These results suggest that simvastatin effects were mainly derived from depletion of intracellular pools of GGPP, the substrate required for the geranylgeranylation. Using this approach, we found that GGPP, a derivative of the mevalonate pathway, is critical for ESC self-renewal. Furthermore, we identified that simvastatin selectively blocked cytosol-to-membrane translocalization of RhoA small guanosine triphosphate-binding protein, known to be the major target for geranylgeranylation, and lowered the levels of Rho-kinase (ROCK)2 protein in ESCs. In addition, simvastatin downregulated the ROCK activity, and this effect was reversed by addition of GGPP. Our data suggest that simvastatin, independently of its cholesterol-lowering properties, impairs the ESC self-renewal by modulating RhoA/ROCK-dependent cell-signaling.</P>