녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

다구찌 방법에 기초한 단면절삭에서 절삭파라미터 영향에 관한 연구

장성민,조명우 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

In this paper, object of experiment is to study on the effect of cutting parameters to obtain optimal surface toughness in face turning. Surface roughness is significantly important to be high quality of parts produced by turning process. For this purpose, the optimization of cutting parameters for face turning operation is investigated applying the Taguchi method. An orthogonal array, signal-to-noise ratio, and the analysis of variance are employed to evaluate effect of cutting parameters for face turning. Also confirmation tests were performed to make a comparison between the results predicted from the mentioned correlations and the theoretical results. Cutting experiment is performed without cutting fluid using coated tungsten carbide inserts about workpieces of SM45C.

실시간 홀로그램간섭계를 이용한 In-Ceram과 금속 하부구조의 변형에 관한 비교 연구

장현주,우이형,이성복 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

Porcelain jacket crown and metal ceramic crown are representative esthetic restorations for full veneer crowns. Genarally, Porcelain jacket crown is more esthetic and closer to natural tooth appearance. However it is brittle and subject especially to tensile stress. Recently, a new reinforced porcelain for substructure, In-Ceram(VITA Zahnfabrik, Germany) was developed. This new reinforced porcelain is stronger than all other existing porcelain material and, can be widely used as one of the substructure materials. This study compared the deformation of In-Ceram and metal substructures for the stress of 5, 7 9, 11, 13, 15kg forced to the each lingual slope with 30 degree angle. Metal substructure was casted with 3 kinds of metals precious(Degudent H, Degussa, Germany), semiprecious(Degupal U, Degussa, Germany), nonprecious(Rexillum IQ, Rexillium, U.S.A.). Real time holographic interferometry was used for analysing the stress of these samples. This holographic interferometric analysis can evaluate the whole aspect of deformation without any morphological limitations and analyze finite deformation of samples by um and can be used on the idential samples not on computer model nor any other simulations. The results obtained were as follows 1. When the load by Max. 15kg was added to the lingual slope of each samples, in all cases of In-Ceram, Degudent H, Degupal U, Rexillium III substructures, tensile deformation on the labial aspect and compressive deformation on the lingual aspect were increaced from the cervical to incisal region. 2. When the surface deformation of each substructure was compared , In-Ceram showed uniform stress distribution without any flexion of its surface. In the metal substructures, Rexillium III on the labial aspect, Degupal U on the lingual aspect showed uniform surface deformation without any flexion. 3. In the case of Degudent H, severe changes of fringe patterns appeared at the incisal resion of labial aspect & whole lingual aspect, therfore interpretation of interferogram was not possible in this study: 4. When compared the Max. deformation of labial surfaces, In-Ceram showed least deformation in the load of 5,7,9kg and also in the load of 11,13,15kg except Rexillium M. When compared the Max. deformation of lingual surfaces, In-Ceram showed greatest deformation in all loading condition. 5. In the respect of stress distribution, In-Ceram was not disadvantageous. Holographic interferometry has a few methodological limit for the small dental restoration anlysis relatively great dynamic force condition. But as noncontact and nondestructive stress analyzing method, it is valuable to analyze 'finite deformation and more studies needed to apply it.

데스크탑 밀링기의 경량 ·고강성화 구조 최적설계

장성현,정우영,홍정표,최영휴 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

This paper describes a case study on the structural design optimization of a desktop milling machine for microfactory. Actually, the structural dynamics analysis is necessary to machine tool design and development to secure high precision machining performance. In the preliminary design stage, one of design alternatives for a desktop milling machine was chosen according to F.E.M structural analysis. It had relatively stronger than others but unfortunately had not enough structural rigidity. After all, we carried out the static design optimization for the chosen design alternative to decrease compliance. The static design optimization problem, in this study, is to find out thickness of structural members which minimize the weight and the static compliance under several constraints. The optimization results showed a great reduction in the compliances, moreover, vibration amplitudes at the spindle nose of this machine tool was also reduced greatly. We successfully designed the machine structure having less static compliance than the critical value.

별렬컴퓨터의 사용자 정의 뷰 생성도구 설계

장경성,방상원,박경우 木浦大學校 情報産業硏究所 1995 情報産業硏究誌 Vol.3 No.-

Parallel Program Visualization has been successfully applied to alleviate the complexities of parallel software development. However, those program visualization tools which utilize the more advanced visualization techniques tend to be too complex and special purpose to be generally useful. The use of tools can be applied to simplify the use of complex environments, but the tools for program visualization can still require extensive specification for views and information processing details. This paper provides a model for program visualization for parallel software that user programmed with his or her defined views. It suggests the dynamic multiprocessing by providing the object code came from dynamic linker.

한국어판 낙상효능 척도와 활동 특이적 균형 자신감 척도의 타장도 및 신뢰도

장숙랑,조성일,오상우,이언숙,백현욱 대한노인병학회 2003 대한노인병학회지 Vol.7 No.4

고선형 단축파 대역 Up-Mixer 설계

장진석,채규성,윤상웅,김창우 慶熙大學校 레이저 工學硏究所 2007 레이저공학 Vol.18 No.-

A single-side band double-balanced mixer using the Gilbert cell structure has been designed with the TSMC 0.18 μm CMOS library. The SSB Up-Mixer consists of a core, IF balun-phase shifter, and LO balun-phase shifter block. In the core block, 4-source-coupled degeneration resistors are used to improve the mixer's linearity. The SSB mixer exhibits a 6.8 dB conversion gain with a - 5.7 dBm P_(1-dB) for IF power of 11.5 dBm and LO power of 0 dBm inputs. The image rejection ratio is - 37.7 dBc at a -5.7 dBm output power.

데스크탑 밀링머신의 고속 스핀들 설계 해석

장성현,황현영,정우영,홍정표,최영휴 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

During a decade, there are many researches on microfactory which is the miniature, flexible, and efficient manufacturing system for the micro/meso scale machining device. Moreover many micro machine tool has developed in Japan, U.S., Europe, and Korea. Already we are developing a desktop milling machine with 380×450×450 ㎣ machine size for microfactory or BT (Bio technology) factory. In this study, we carried out the design analysis of a high speed spindle system for our desktop milling machine by using ADROS, which is our own developed S/W for dynamic design analysis of rotor systems. As the result, we obtained some analyzed dynamic characteristics of a spindle system, such as natural frequency, whirl frequency, critical speed, and harmonic response.