Biochemical Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in Korea

Ki-seuk Kim,Young-ju Lee,Yong-kuk Kwon,Ryun-bin Tak 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Characteristics and Antimicrobials Susceptibility of Salmonella gallinarum Isolated in KoreaYoung-ju Lee, Ki-seuk Kim1,*, Yong-kuk Kwon and Ryun-bin Tak1Avian Disease Division, National Veterinary Research and Quarantine Service, Anyang 430-824, Korea1College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived February 13, 2003 / Accepted April 30, 2003J. Vet. Sci. (2003), 4(2), 161-166JOURNAL OFVeterinaryScience*Corresponding author: Ki-seuk Kim College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea Tel: 82-53-950-5962, Fax: 82-53-950-5955 E-mail: kimkiseuk@knu.ac.kr

특별기고 : 비의사 전문 유전상담사의 교육 및 자격의 인증을 위한 소고

김현주 ( Hyon J. Kim ),도성탁 ( Seong Tak Doh ) 대한임상검사과학회 2009 대한임상검사과학회지(KJCLS) Vol.41 No.3

This short review was aimed to provide the information for the people who are interested in genetic counselor education and certification system in Korea. A large part of this study is indebted to HJ Kim``s articles on the genetic counselor system, the global standards of genetic counseling curriculums, training program accreditation (TPA), and a certification process for genetic counselors (CPGC) in the US and Japan. The US and Japanese educational systems showed a high degree of similarities in curriculum, accreditation, and certification programs. Based upon this review, we hereby propose that the Korean Society for Medical Genetics should take a key role in providing the TPA and CPGC for non-MD genetic counselors. Requirement for the entrance to a Master``s degree genetic counseling program should be open to successful four year undergraduate students for all areas, provided the candidates demonstrate the abilities to master the graduate level study in human genetics, statistics, psychology, and other required subjects. Besides accredited program graduates, eligibility for certification should also include the qualified candidates of genetic counseling with no formally approved education, but with a sufficient amount of clinical experience.

조현병 환자의 언어성 환청과 정신병리의 PANSS 요인들 간의다차원적 관계

신샘이,김세현,이남영,윤탁,김용식,정인원,Shin, Sam Yi,Kim, Se Hyun,Lee, Nam Young,Youn, Tak,Kim, Yong Sik,Chung, In Won 대한생물정신의학회 2015 생물정신의학 Vol.22 No.4

Objectives This study was aimed to examine the multidimensional relationship between auditory verbal hallucinations (AVHs) and Positive and Negative Syndrome Scale (PANSS) factors of psychopathology in the patients with schizophrenia. And we explored the differences between assessments to hallucination by the clinicians and patients. Methods 82 patients with schizophrenia who were assessed by the Hamilton Program for Schizophrenia Voices Questionnaire (HPSVQ), Psychotic Symptom Rating Scale-Auditory Hallucination (PSYRATS-AHS), and the PANSS were recruited. Hwang's five-factor model of PANSS, items and total scores of hallucination scales, Kim's and Haddock's factor models of hallucination were applied to examine the correlations between psychopathology and AVHs. AVH-positive patients was 50 in PANSS-HPSVQ group and 24 in PANSS-PSYRATS-AHS. These two groups were separately analyzed. Results Among the five factors of the PANSS, negative and depression/anxiety factors were correlated with the total scores of HPSVQ and PSYRATS-AHS, and positive and autistic preoccupation factors were correlated only with the total score of PSYRATS-AHS. The activation factor was correlated with none of the total scores of HPSVQ/PSYRATS-AHS. These correlation patterns of a total score of HPSVQ/PSYRATS-AHS were same in the emotional factor of HPSVQ and physical factor of PSYRATS-AHS respectively. In the items which showed significant correlations, correlation coefficients of PANSS-PSYRATS-AHS group ranged between 0.406-0.755 and those of PANSS-HPSVQ ranged between 0.283-0.420. Conclusions This study suggested that the psychopathological domains of schizophrenia were differentially correlated with AVHs and the assessment of AVHs by clinicians and patients showed substantial differences which should be integrated into the therapeutic interventions.

유기인제 급성폭로후에 발생한 지연형 신경장애 1례

김탁관,이동철,김상범 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

A relatively healthy 64-year-old man who ingested organophosphorous insecticide in an attempt to commit suicide was brought to the emergency room by his family members showing mental change and respiratory arrest. He suffered from delayed onset of generalized muscle weakness, body balance loss and respiratory difficulty that developed on 3 days after admission, and then he was supported by mechanical ventilation. We concluded that his final diagnosis is delayed neurotoxicity after carefully examining his neurologic status and electromyography. We have no data from nerve and muscle biopsy but we think delayed neurotoxicity in present due to compatible findings of clinical features and electrodiagnosis report.

실시간 신경망 적응 노이즈 필터 개발을 위한 기초설계

김진선,장탁순,홍준희 충남대학교 공업교육연구소 2003 論文集 Vol.26 No.1

This Paper describes a nonlinear adaptive noise canceller using neural network for machine tools controller system. Back-Propagation Learning Algorithm based MLP(Multi Layer Perceptron) is used an adaptive filters. In this paper, it assume that the noise of primary input in the adaptive noise canceller is not the same characteristic as that of the reference input. Experimental results show that the neural network base noise canceller outperforms the linear noise canceller. Especially to make noise cancel close to realtime, Primary Input is divided by Unit and each divided part is processed for very short time than all the processed data are unified to whole data.

CC Chemokine CKβ8의 발현 조절 물질 탐색 및 저해활성 물질 분석

김탁용,이태훈,백남인,정대균,김지영 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

CKβ8은 CC 케모카인의 부류에 속하는 사이토카인의 일종으로, 단핵구, 호염구, 호산구, T 림프구, 자연살해세포, 수지상세포 등의 화학주성물질로 작용하는 것으로 알려져 있다. CKβ8은 염증반응 및 혈관신생 등과 같은 여러 가지 생체 반응에 역할을 하는 것으로 알려져 있다. 본 논문에서는 사람 CKβ8 유전자의 전사저해 물질을 선별하기 위하여 CKβ8/Luc 유전자를 발현하는 세포주를 확립하였다. CKβ8 유전자의 전사시작점에서 -299 부터 +10 프로모터 부위를 PCR에 의해 얻은 다음 포유세포 발현 플라스미드에 클로닝하였다. CKβ8/Lvc 유전자는 U937 세포내에서 PMA에 의해 10배 이상 발현이 증가한다. CKβ8/Luc 발현 U937 세포주는 식물 추출물에서 전사 저해제를 탐색하는데 이용되었다. 46 개의 식물 추출물 중에서 6개가 90% 이상 CKβ8/Luc의 전사를 저해하였다. 이들 물질에 대해 세포 독성 실험을 수행한 결과 그 중 한 개만이 세포 독성이 거의 없는 것으로 나타났다. 이 추출물은 붉나무에서 얻은 추출물이며 이 식물 추출물은 염증관련 질환에 대한 가능성이 있는 치료제로 이용될 수도 있을 것으로 추측된다. Ckβ8 belongs to a family of CC chemokines, which is known to act as a chemoattractant for monocytes, basophils, eosinophils, T lymphocytes, NK cells, and dendritic cells. CKβ8 is known to play roles in various biological processes such as inflammation and angiogenesis. In the present study, we developed U937 cell line expressing CKβ8/Luciferase fusion gene for screening of transcriptional inhibitors for human CKβ8 gene. Genomic sequences that contain CKβ8 promoter region from 299 to+10 nucleotide from the transcription start site was amplified by PCR and cloned into mammalian episomal plasmid. The CKβ8/Luc fusion plasmid was introduced into human promonocytic U937 cells and selected for stable U937 cells expressing the fused reporter gene. The expression of the fusion gene was induced by more than 10 folds upon treatment of phorbol myristate acetate. The stable U937 transfectant cells were used for searching transcriptional inhibitors for human CKβ8 gene from plant extracts. Among 46 plant extracts tested, six extracts exerted more than 90% inhibition of PMA-induced CKβ8 transcription. Cytotoxic tests demonstrated that one of extracts, which was extracted from Rhus chinensis Mill, had little cytotoxic effect. The extract may be used for developing a potential therapeutic agent for inflammatory diseases.

HS-SPME-GC/MS 방법을 이용한 실크벽지 원자재의 TVOCs 기여도 평가

정탁교,장미옥,김만구 강원대학교 부속 환경연구소 2005 Journal of the Environment Vol.2 No.-

The contributions of the TVOC toluene-equivalent emission of raw materials was investigated for PVC wallpaper. A Headspace of each raw material were concentrated by solid-phase microextraction (SPME) and analysed by GC/MS. Static headspace of PVC wallpaper was generated in the 22ml of glass vial at 25℃ and sampled by using a 75 um thickness of carboxen-polydimethylsiloxanfiber. The typical VOCs are oilic peaks eluted between n-decane and n-hexadecane, and carbonyl compound, 2-butanone, n-hexanal, 2-ethyl-hexanol, 4-tetradecanal, hecanoic acid and aromatic compounds, toluene, m,p-xylene, styrene, Dileunt showed the highest contribution to TVOC emission of 0.52, and plasticizer was 0.20, and resin stabilizer showed 0.14. the improvement of these raw material was recommended in odor to reduce TVOC emission of PVC wallpaper.

신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

PS 판용 Naphthoquinone-(1,2)- diazide-5-sulfonic acid esters의 합성 및 응용

홍장후,정택서,김운겸 서울産業大學校 1999 논문집 Vol.50 No.2

본 연구는 Sodium-2-diazo-napthoquinone-5-sulfonate를 chlorination하여 2-diazo-naphthoquinone-5-sulfonylchloride(NQD-Cl)를 합성하였으며 합성된 NQD-Cl을 여러 종류의 hydroxybenzophenone 유도체와 esterification하여 hydroxy group이 2-diazo-napthoquinone-5-sulfonyl(NQD) group으로 치환된 여러 종류의 naphthoquinone-(1,2)-diazide-5-sulfonic acid esters(NQD-ester)를 합성하였다. NQD-ester의 용해도를 증가시키기 위해 methoxy group이 도입된 유도체와 이와 비교를 위해 hydroxy group만을 가지는 benzophenone 유도체를 사용하여 비교 검토하였다. 합성된 각각의 NQD-ester의 용해특성을 조사하였으며, novolac수지와 formulation하여 감광액을 제조하였다. 제조된 감광액을 알루미늄판에 도포, 건조하여 PS판을 제조하고 감광특성과 광퇴색도와 화상형성에 적합한 노광시간을 조사하였으며, 상대감도를 gray scale(GS)법으로 조사하였다. 치환된 NQD group의 수에 따라 GS법에 의한 상대감도가 다르게 나타나는 것을 알 수 있었다. Methoxy group이 도입된 NQD-ester는 좋은 용해특성을 보여주었으며 시판되는 PS판과 동일한 노광조건에서 비교해 본 결과 보다 우수한 감도를 보였다. 2-Diazo-l-naphthoquinone-5-sulfonyl chloride(NQD-Cl) was synthesized from Sodium-2-diazo-napthoquinone-5-sulfonate by chlorination. Synthesized NQD-Cl was esterified with hydroxybenzophenones to give several naphthoquinone-(1,2)-diazide-5-sulfonic acid esters(NQD-esters). We are investigated comparing benzophenone derivatives with methoxy group and benzophenone derivatives with hydroxy group. Solubility of each NQD-esters was studied and each of NQD-esters was formulated w novolac base resin and PS plates were manufactured. Photosensitivity, bleachability, compatible exposed time and relative sensitivity were determined by UV spectro photometry, imaged by UV lithographic techniques and grey scale method. According to the number of substituted NQD group, it showed that relative sensitivity was different from grey scale. NQD-esters with methoxy group were shown a good solubility and higher sensitivity than commercial PS ones.

아미노산 투여가 중장거리 운동선수의 최대 및 최대하운동시 젖산생성에 미치는 영향

이정수,윤탁영,김문희,이선장,김완조 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

The Purpose of the Study was to determine whether the glutamate ingestion prior to exercise would reduce the lactat formation and its clearance in exercising mscle tisue during Submaximal and maximal execise. Six elite distance rummers particapated in this study. The Subjects had a half dose of glutamale(15.6mg .Kg?? ??) at 45min and ingested rest dose at prior to exerise. The test Performed twice. One was Performed in the track and the other was in the Lab. Blood Smples were obtained from catheter which was placed in the antecubital vein (Lab test) and finger tip Samples (Feld test) Unaltered blood lactate was observed in both trials, but, tended to less lactate concentration with glutamate ingestion than that of without glutamate ingestion. The recovery rate of blood lactate was markely rapid with glutamate ingestion in the field test. Unchanged in heart rate was founded in both trials. It many Suggest that, the additional supplement st of glutamate can improve execise performance limiting lactate formation or induce its rapid clearance from the muscle fissue.