이단계 연속배양시 세포 재순환의 영향

박태현 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

Uncoupling of the cell growth from the production of an inducible substance gives higher performance, and two-stage continuous operation allows one to separate the cell growth from the product induction. Residence time of each individual cell in the second tank is important since the extent of the induction is dependent on the residence time of the cell in the second tank and keeps the cell to stay long enough for the product induction. The effect of cell recycle on the residence time in two-stage continuous operation has been theoretically analyzed. The cell recycle system increases the mean intracellular concentration by the increment of the mean residence time of the cell. Higher recycle ratio and lower separation parameter give longer mean residence time. Optimum dilution rate exists for the maximum productivity.

재순환 실관 효소 반응기 시스템에서 엄밀수치해와 근사해석해의 비교(Ⅱ)

박태현 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

Hollow fiber membrane devices have been used as enzyme reactors because of their large surface area-volume ratio and the advantage of immobilized soluble enzymes in intact form. Theoretical analysis of the hollow fiber enzyme reactor with recirculated enzyme solution was performed and the model equations were numerically solved in previous paper. For a slow kinetics one might assume that the flowing substrate solution has radially uniform concentration profile. In this paper, the equations were solved analytically with the above assumption in the case of 1st and zero order reaction, and the approximate analytical solutions were compared with the exact numerical solutions. Exact numerical solutions of shell side concentration and of the outlet concentration at low Pe'clet number were in good agreement with the approximate analytical solutions. And the two solutions of outlet concentration at high Pe'clet number were agreed, when the Thiele modulus was small.

재순환 실관 효소 반응기의 이론적 해석(Ⅰ)

박태현 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

Hollow fiber enzyme reactor has evolved as a spin-off of the rapidly developing membrane technologies. Hollow fiber module is now favorably considered as enzyme reactor, artificial kidney, ultrafiltration device, reverse osmosis device, and gas separator due to its high area-to-volume compared with other membrane modules such as flat or spiral modules. However, when an enzyme is immobilized in a sponge annulus, an ultrafiltration causes washout of the enzyme from the sponge annulus to result in a nonuniform distribution along the fiber. Thus, utilization of the retained enzyme is not adequate. However, this problem can be solved by recirculating the enzyme solution in the shell side, and also mass transfer resistance can be reduced by introducing the ultrafiltration. In this paper, the continuous system with recirculated enzyme solution was modeled. The numerical results were in good agreements with those of experiment. When Thiele modulus of the porous sponge region is less than 1, reaction occurs also in the shell side.

발효시 세포 재순환의 새로운 응용

박태현 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

Cell recycle has been used in fermentation and most of the previous works were concerned with increasing the cell concintration. Other utilities of cell recycle chemostat are considered in this article. Steady state should be changed from one to another in the experiment for the evaluation of kinetic parameters. In this case, the recylce system reduces the transient period between two steady states. The effect of cell recycle on the trasient period in single-stage continuous operation is theoretically analyzed. Dilution rate or another parameter, r, can be varied to get other steady states. The result shows that change of the dilution rate rather than r gives shorter transient period. For the same specific growth rate, combination of higer dilution rate and lower r gives shorter transient period.

Streptomycin 耐性 肺炎桿菌의 Phage 血球凝集反應 形成能力

朴寶漢,安泰烋,李炫哲,辛富安 全南大醫大 1983 전남의대학술지 Vol.20 No.1

저열량영양식품 섭취에 따른 비만여성의 체중감량 효과에 관한 연구

박순영,최중명,김동현,최경식,최봉근,윤태영,유동준 한국보건통계학회 2002 보건정보통계학회지 Vol.27 No.1

In the method of obesity treatment, Dietary method, Exercise method, Behavior method, and Drug method, Dietary method is the most important control method of obesity. The aim of this study is that evaluate the weight reduction effect of the specific low calorie diet products (KSD I and 2) in obese women. Twenty five women who were volunteer participated in this study. They have no heart diseases and metabolic diseases. we classified two groups : obese group and standard group. Anthropometric indices were measured in all subject for four times. Venous blood sample were taken to determine the lipid profiles, glucose level, and liver function test. In obese group, body weight were reduced 2.85±1.46kg, but showed statistically non-signicicant. Body mass index were gradually reduced, 1.09±0.93kg/m^2(p<0.05).In blood analyses, total cholesterol were reduced 10.61 ±33.4mg/dℓ ( p<0.05). KSD 2 significantly inhibited maltase, sucrase and α-glucosidase from rat intestine, and porcine pancreatic lipase, although KSD I inhibited only the sucrase.

피브로인 생산을 위한 누에 후부견사샘의 기관배양

박수연,하성호,김재현,박태현 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.2

Posterior silk glands of Bombyx mori were cultured for the in vitro production of silk fibroin. Four-day-old fifth-instar larvae and Grace's media with or without silkworm hemolymph were used for experiments. With the addition of silkworm hemolymph, significant increase in the secretion of fibroin from the lumen to the outside of the gland was observed and total amount of fibroin increased by 14%. Most of the fibroin produced in the media was insoluble protein. Total amount of fibroin reached a plateau in 24 day culture without the addition of silkworm hemolymph, whereas it increased continuously in the medium supplemented with silkworm hemolymph.

도시 하수오니에 의한 고분자 물질의 생분해도 측정

박현호,박태현 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.2

Factors influencing the biodegradability of polymer materials by municipal sewage sludge were studied using starch, polyethylene glycol(PEG) and polyvinyl alcohol(PVA). The degree of biodegradability was determined by measuring evolved carbon dioxide as a function of time, when the polymer material is exposed to activated sewage sludge. The biodegradability of starch reached around 70% and 40% after 30day incubation in summer and autumn, respectively. This is caused by the activity of microorganism and incubation temperature. The biodegradability of PEG 8000 and PVA was 1/7 and 1/14 of starch, respectively. Biodegradation of PEG 8000 after 30 day incubation in summer was slightly higher than that in spring while lag phase was significantly longer in spring. This shows that the activity of microorganism as well as incubation temperature is important for biodegradation.

물리교육의 효율적인 교수-학습 지도 방안 : 빛 단원을 중심으로 learning methods of physics education

강현식,박승태,양동익 全北大學校 敎育大學院 1983 敎育論叢 Vol.3 No.-

Teaching physics education, various materials have been used. But many kinds of equipments are not sufficient and their operations are very complecate. On the other hand, it takes a lot of time for preparations. The physical experiments should decide the directions of lectures and motivate the learning students. The most remarkable discovery made by physical science is physical phenomena itself. The central problem in teaching physics today is to establish the principles of physics which consist of the basis of all physical phenomena. And the demonstration experiments are used frequently inside and outside the classrooms, and the operations of experiments are convinient to impulse of interests of students. In order to update the teaching and learning method of physics education effectively, we have studied“LIGHT”in high school's physics using improved method by the experiments though the analysis and investigation of the measuring results.

태권도 품새 수련이 아동의 심박수, 심근산소소비량, 에너지 대사에 미치는 영향

정성태,전태원,박익렬,정덕조,우재홍,이광희,이동기,강현주 서울대학교 체육연구소 2000 서울大學校 體育硏究所論集 Vol.21 No.1

The Taegeuk Poomse 1 through 8 of Taekwondo are based upon eight major branches of philosophical theories. The propose of this study was to investigate the nfluence of Taekwondo poomse trainning on heart rate, MVO2, energy metabolism in primary school students. Subjects for this study were eight male the fifth∼sixth grades students who participated in exercise program consist of Taekwondo poomse trainning in dojang which is located in Shinlim-2dong, Kwanak-Gu, Seoul(12.7± 0.5years, 150.8 ±4.6cm, 45.1 ± 9.4kg, 24.9 ± 9trainning length). The results of thet this study are as follows: 1) The average of heart rate was Taegeuk Poomse 1 through 8 of Taekwondo 90.57± 4.gbeats/min in rest and 139.7 ± 17.5beats/min in exercise. The minimum heart rate was 89.0±2.8, peak heart rate was 167.1 ±6.4 in exercise. It was ranged from 42% to 80% 2) of the max heart rate and ranged 42 to 65%HRmax. Taegeuk 1 Jang was differenced with others Jangs significantly(p<0.05), 2) The time of the Taegeuk 1 through 8 Jang of Taekwondo was 5minutes 12seconds. Heart rate was ranged from 90.6 ± 7.85beats/min to 171.8 ±8.Ibeats/min and increased linearly. 3) The average of the MVO2 was 11.1 ±0.78mmHg · bpm-3 in rest and 19.1 ± 1.2mmHg ㆍbpm-3 in exercise. MVO2 was ranged from 10.5mmHg · bpm-3 to 20.3mmHg · bpm-3. There were not difference between Taegeuk Poomses(p<0.05). 4) The energy metabolism was as follows: Mean VO2 Respiratory exchange ratio, energy consumption were 0.51(V02L/min),0.71(RQ),2.32Ckca1/min) in rest respectively. but In exercise Mean VO2 Respiratory exchange ratio, energy consumption were 0.89 ± 0.18( V 02L/min), 0.79±0.08(RQ), 4.26±0.51(kcal/min) respectively . The difference was only between Taegeuk 1 Jang and Taegeuk 3 Jang(p<0.05). This study showed that the Taekwondo Poomse trainning was proper exercise for fitness, body composition and weight control in normal, abnormal(obese) children because the Taekwondo trainning are dynamic and exciting sport.