[PD-0019] Expression of colorectal cancer antigenic protein fused to IgM Fc for plant cell factory system using Chinese cabbage (Brassica rapa)

Do-Sun Kim(Do-Sun Kim),Ye-Rin Lee(Ye-Rin Lee),Kisung Ko(Kisung Ko),Taewon Yang(Taewon Yang),Seung-Won Lee(Seung-Won Lee),Sang-Hyun Moh(Sang-Hyun Moh),Hye-Eun Lee(Hye-Eun Lee),Eun Su Lee(Eun Su Lee),Ko 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Enhanced UV stability of perovskite solar cells with a SrO interlayer

Lee, Sang-Won,Kim, Seongtak,Bae, Soohyun,Cho, Kyungjin,Chung, Taewon,Hwang, Jae-Keun,Song, Inseol,Lee, Wonkyu,Park, Sungeun,Jung, Jaebong,Chun, Jihun,Lee, Yoon Jung,Moon, Yeon Ji,Lee, Hae-Seok,Kim, Do Elsevier 2018 Organic Electronics Vol.63 No.-

<P><B>Abstract</B></P> <P>We investigated strontium oxide (SrO) as an interlayer material to enhance the UV stability of a CH<SUB>3</SUB>NH<SUB>3</SUB>PbI<SUB>3</SUB> perovskite solar cell. Moisture and over 400 nm wavelength of light were excluded to investigate the effect of UV light only. Two different interlayer fabrication processes were examined to optimize the performance of this solar cell. Devices fabricated by dipping for 30 min in SrO solution exhibited photoconversion efficiencies of 15.5%, whereas those fabricated with 60-min dipping showed photoconversion efficiencies of 15% and exhibited local Sr agglomeration. Devices with SrO displayed lower initial efficiencies than those without any SrO layer (17.6%), However, a device without SrO retained only 34.4% of its initial efficiency after 100 h of UV exposure. In contrast, SrO-incorporated devices retained almost 60.0% of their initial efficiency. Severe μ-PL mapping intensity degradation was observed in devices that did not include the interlayer, but no degradation was observed in those with the SrO interlayer. This can be attributed to the passivation of the degradation sites by SrO.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Perovskite UV stability was tested under conditions except moisture, oxygen and wavelengths exceeding 400 nm. </LI> <LI> With high resolution μ-PL mapping technique, direct evidence is provided about the area perovskite UV degradation begins. </LI> <LI> UV degradation is initiated at the ETL/perovskite interface. </LI> <LI> Effect of the SrO interlayer on UV stability was examined in an inert atmosphere. </LI> <LI> UV stability is enhanced by SrO interface passivation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A Feasibility Study of Low-Dose Single-Scan Dual-Energy Cone-Beam CT in Many-View Under-Sampling Framework

Lee, Donghyeon,Lee, Jiseoc,Kim, Hyoyi,Lee, Taewon,Soh, Jeongtae,Park, Miran,Kim, Changhwan,Lee, Yeon Ju,Cho, Seungryong IEEE 2017 IEEE transactions on medical imaging Vol.36 No.12

<P>A single-scan dual-energy low-dose cone-beam CT (CBCT) imaging technique that exploits a multi-slit filter is proposed in this paper. The multi-slit filter installed between the x-ray source and the scanned object is reciprocated during a scan. The x-ray beams through the slits would generate relatively low-energy x-ray projection data, while the filtered beams would make high-energy projection data. An iterative image reconstruction algorithm that uses an adaptive-steepest-descent method to minimize image total-variation under the constraint of data fidelity was applied to reconstructing the image from the low-energy projection data. Since the high-energy projection data suffer from a substantially high noise level due to the beam filtration, we have developed a new algorithm that exploits the joint sparsity between the low-and high-energy CT images for image reconstruction of the high-energy CT image. The proposed image reconstruction algorithm uses a gradient magnitude image (GMI) of the low-energy CT image by regularizing the difference of GMIs of the low-and high-energy CT images to be minimized. The feasibility of the proposed technique has been demonstrated by the use of various phantoms in the experimental CBCT setup. Furthermore, based on the proposed dual-energy imaging, a material differentiation was performed and its potential utility has been shown. The proposed imaging technique produced promising results for its potential application to a low-dose single-scan dual-energy CBCT.</P>

고해상도 고용량 지형 DB 탑재 실시간 정밀 지형대조항법이 가능한 DTED 처리기법

김태원(Taewon Kim),오주현(Ju-Hyun Oh),이상훈(Sanghoon Lee),노형우(Hyoung Woo Noh),김승진(Seungjin Kim),이기웅(Kee-Woong Lee),남성호(Seongho Nam),한경준(Kyung-Jun Han),이정신(Jung-shin Lee),박병수(Byung-Su Park),이형섭(Hyung-Sub Lee) 한국항공우주학회 2018 한국항공우주학회 학술발표회 논문집 Vol.2018 No.11

Magnetic property (T_<i>c</i> ∼ 300 K) originated from InZnP:Ag nano-rods fabricated with noble metal Ag using ion milling method

Lee, Juwon,Shon, Yoon,Subramaniam, N.G.,Kang, Taewon,Sohn, Jae Min,Kim, Hyungsang,Im, Hyunsik,Koo, Hyun Cheol,Lee, Joo-hyeon,Song, Jing Dong,Park, Chang-Soo,Kim, Eun Kyu Elsevier 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.704 No.-

<P><B>Abstract</B></P> <P>The well aligned InZnP:Ag nano-rods doped with noble metal Ag were fabricated using ion milling method. Results for XPS coincide with results for AES, especially Ag 3d5. The uniformity, density, and height of InZnP:Ag nano-rods were well formed in TEM measurements. In order to clarify the formation of InZnP:Ag nano-rods, SEM was carried out due to the doping of Ag. XRD measurements were performed in order to support the above TEM results, it is confirmed that the lattice was well aligned without distortion and that the nano-rod was a single crystalline zinc blende structure. The variation of FWHM for TAD peaks demonstrates that noble metal Ag is incorporated into InZnP:Ag nano-rods. The trend of FWHM results coincides with the trend of TEM results according to ion milling depth. The clear opening of the ferromagnetic hysteresis loop indicates the formation of ferromagnetic spin coupling interaction in InP:Zn doped with Ag. The FC M-T curve clearly displays that the ferromagnetism of InZnP:Ag persists near 300 K.</P> <P><B>Highlights</B></P> <P> <UL> <LI> InZnP:Ag nano-rods were fabricated using useful ion milling method. </LI> <LI> Development for the fabrication of 1D nano-rods using noble metal silver (Ag). </LI> <LI> The magnetic properties of InZnP:Ag nano-rods were further investigated for spintronic application. </LI> <LI> M-H and M-T curves clearly display that the ferromagnetism of InZnP:Ag persists up near 300 K. </LI> </UL> </P>

A highly Lewis acidic triarylborane bearing peripheral <i>o</i>-carborane cages

Lee, Kang Mun,Huh, Jung Oh,Kim, Taewon,Do, Youngkyu,Lee, Min Hyung Royal Society of Chemistry 2011 Dalton Transactions Vol.40 No.44

<P>A triarylborane (2) bearing three <I>o</I>-carborane cages at peripheral positions on the aryl groups was prepared and its crystal structure was determined from X-ray diffraction study. Treatment of 2 with KF in the presence of 18-crown-6 led to the potassium salt, [2F]<SUP>−</SUP>. A UV-vis titration experiment carried out in THF/H<SUB>2</SUB>O (9/1 v/v) showed that 2 binds fluoride ions with a binding constant (<I>K</I>) of 4.8 × 10<SUP>4</SUP> M<SUP>−1</SUP>, which is an order-of-magnitude greater than <I>K</I> for the mono-carborane substituted triarylborane. The enhanced fluoride ion affinity of 2 indicates an apparent additive effect of multiple carborane substitutions on the Lewis acidity enhancement of the triarylborane. The highly Lewis acidic nature of 2 was further utilized in evaluating the fluoride ion affinity of tris(pentafluorophenyl)borane (B(C<SUB>6</SUB>F<SUB>5</SUB>)<SUB>3</SUB>). A fluoride exchange reaction between [2F]<SUP>−</SUP> and B(C<SUB>6</SUB>F<SUB>5</SUB>)<SUB>3</SUB> resulted in 15 times higher fluorophilicity for B(C<SUB>6</SUB>F<SUB>5</SUB>)<SUB>3</SUB> than for 2. The lower Lewis acidity of 2 compared with B(C<SUB>6</SUB>F<SUB>5</SUB>)<SUB>3</SUB> was confirmed from its greater cathodic reduction potential.</P> <P>Graphic Abstract</P><P>The introduction of three <I>o</I>-carborane moieties into a triarylborane shows an apparent additive effect on Lewis acidity enhancement of the triarylborane. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1dt11064k'> </P>

In situ observation of atomic movement in a ferroelectric film under an external electric field and stress

Lee, Hyeon Jun,Guo, Er-Jia,Min, Taewon,Hwang, Seung Hyun,Lee, Su Yong,Dorr, Kathrin,Lee, Jaekwang,Jo, Ji Young Tsinghua Press, co-published with Springer-Verlag 2018 NANO RESEARCH Vol.11 No.7

<P>Atomic movement under application of external stimuli (i.e., electric field or mechanical stress) in oxide materials has not been observed due to a lack of experimental methods but has been well known to determine the electric polarization. Here, we investigated atomic movement arising from the ferroelectric response of BiFeO3 thin films under the effect of an electric field and stress in real time using a combination of switching spectroscopy, time-resolved X-ray microdiffraction, and in situ stress engineering. Under an electric field applied to a BiFeO3 film, the hysteresis loop of the reflected X-ray intensity was found to result from the opposing directions of displaced atoms between the up and down polarization states. An additional shift of atoms arising from the linearly increased dielectric component of the polarization in BiFeO3 was confirmed through gradual reduction of the diffracted X-ray intensity. The electric-field-induced displacement of oxygen atoms was found to be larger than that of Fe atom for both ferroelectric switching and increase of the polarization. The effect of external stress on the BiFeO3 thin film, which was controlled by applying an electric field to the highly piezoelectric substrate, showed smaller atomic shifts than for the case of applying an electric field to the film, despite the similar tetragonality.</P>

Moving Beam-Blocker-Based Low-Dose Cone-Beam CT

Lee, Taewon,Lee, Changwoo,Baek, Jongduk,Cho, Seungryong Professional Technical Group on Nuclear Science 2016 IEEE transactions on nuclear science Vol.63 No.5

<P>This paper experimentally demonstrates a feasibility of moving beam-blocker-based low-dose cone-beam CT (CBCT) and exploits the beam-blocking configurations to reach an optimal one that leads to the highest contrast-to-noise ratio (CNR). Sparse-view CT takes projections at sparse view angles and provides a viable option to reducing dose. We have earlier proposed a many-view under-sampling (MVUS) technique as an alternative to sparse-view CT. Instead of switching the x-ray tube power, one can place a reciprocating multi-slit beam-blocker between the x-ray tube and the patient to partially block the x-ray beam. We used a bench-top circular cone-beam CT system with a lab-made moving beam-blocker. For image reconstruction, we used a modified total-variation minimization (TV) algorithm that masks the blocked data in the back-projection step leaving only the measured data through the slits to be used in the computation. The number of slits and the reciprocation frequency have been varied and the effects of them on the image quality were investigated. For image quality assessment, we used CNR and the detectability. We also analyzed the sampling efficiency in the context of compressive sensing: the sampling density and data incoherence in each case. We tested three sets of slits with their number of 6, 12 and 18, each at reciprocation frequencies of 10, 30, 50 and 70 Hz/rot. The optimum condition out of the tested sets was found to be using 12 slits at 30 Hz/rot.</P>

Salt stress affects global protein expression profiles of extracellular membrane-derived vesicles of <i>Listeria monocytogenes</i>

Lee, Taewon,Jun, So Hyun,Choi, Chi Won,Kim, Seung Il,Lee, Je Chul,Shin, Ji Hyun Elsevier 2018 Microbial pathogenesis Vol.115 No.-

<P><B>Abstract</B></P> <P>Our previous study has suggested that <I>Listeria monocytogenes</I> produces extracellular membrane vesicles (MVs) and its general stress transcription factor sigma B (σ<SUP>B</SUP>) affects the production of MVs under energy stress. The objective of this study was to evaluate the production of MVs and perform global protein profiling for MVs with or without salt stress to understand the function of MVs in the pathogenesis of <I>L. monocytogenes</I>. When cells of <I>L. monocytogenes</I> were grown under 0.5 M salt stress, protein concentrations of MVs derived from wild-type strain and its isogenic Δ<I>sigB</I> mutant were approximately doubled compared to those of MVs derived from cells without salt stress. Proteomic analyses showed that the number of MV proteins expressed in wild-type strain was similar to that in Δ<I>sigB</I> mutant under salt stress. However, global protein expression profiles were dramatically changed under salt stress compared to those without salt stress. Fifteen σ<SUP>B</SUP> dependent proteins were expressed in MVs of wild-type strain under salt stress, including osmolyte transporter OpuCABCD. In addition, MVs produced by salt stressed wild-type and Δ<I>sigB</I> mutant inhibited biofilm formation abilities of both strains. Taken together, our results suggest that salt stress can promote the production of MVs involved in carnitine transporter proteins, with σ<SUP>B</SUP> playing a pivotal role in biological event.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Osmolyte transpoter proteins are packed in MVs from salt stressed <I>L. monocytogenes.</I> </LI> <LI> OpuCABCD regulated by σ<SUP>B</SUP> are important MV proteins obtained under salt stress. </LI> <LI> MVs obtained from salt stress do not contribute to biofilm forming ability. </LI> </UL> </P>

Development of chinese cabbage cell plant factory for biopharmaceutical production

Taewon Yang,Seung-Won Lee,Ye-Rin Lee,Yang Joo Kang,Kibum Kim,Chae-Yeon Lim,Hyunjoo Hwang,Do-Sun Kim,Kisung Ko 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07

EpCAM is a tumor-related antigen and is a cell surface glycoprotein expressed in colon cancer. EpCAM has been considered a potential target for tumor vaccines. In this study, Agrobacterium-mediated transformation was applied to produce genetically modified Chinese cabbage plants to express EpCAM-IgM FcK. To avoid the plant-specific N-glycosylation process occurring in Golgi devices, the KDEL ER sequence was attached to the C-terminal of Fc to refair the glycoprotein of ER. The recombianat protein productivity was maximized by developing a safe and efficient plant cell factory system using a genetically modified plant cell line expressing EpCAM-IgM FcK protein. In addition, plant seedlings expressing both EpCAM-IgM FcK and J-chain K were obtained by crossing. PCR confirmed that the EpCAM-IgM FcK gene present in the seedlings. Western Blot screened seedlings with high expression. Chinese cabbage protoplasts were obtained by successfully optimizing the protoplast separation protocol to perform single cell transcription analysis. Single cell transcription analysis was performed to reveal the cell types that highly express EpCAM-IgM FcK protein. Cell lines with high expression will be applied to plant cell factory systems to produce EpCAM-IgM FcK.