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Taeman Han(Taeman Han),Seung-Chul Park(Seung-Chul Park),Haechul Park(Haechul Park) 국립공원연구원 2022 국립공원연구지 Vol.13 No.1
A Korean indigenous species, Protaetia brevitarsis seulensis (PBS), is popularly reared at many insect farms as valuable industrial insect resources in South Korea. However, there is no comprehensive study on the genetic diversity within the Korean PBS population as well as between morphologically closed species. Employing the rapidly evolved genetic markers such as mitochondrial DNA is important in the detection of haplotype diversity and rapid species identification of larval stage and/or its processed goods. We here examined how many genetically different haplotypes distribute in the reared PBS collected from 14 insect farms and the interspecific genetic difference between congeners using mitochondrial COI gene sequences (DNA barcoding region). As a results, the PBS was clearly separated from two related species with large genetic distances (7.98%~8.86%). In the PBS, 57 haplotypes were detected from 223 specimens with low intraspecific genetic distance (~1.3%). Among them, the most dominant haplotypes are subsequently detected in H4 (25.6%), H38 (15.7%) and H26 (10.3%). From the haplotype network and distribution analyses, we consider that many haplotypes have been mixed by artificial collections from various local places and exchanged between insect farms within South Korea. We expect that this data will be helpful in rapid species identification for its processed goods, and understanding the exact population diversity and detection of the original habitats for each haplotype of the reared PBS throughout comparing with natural populations in the further study.
DNA Barcoding from Old Insect Specimens
Taeman Han,Tae Hwa Kang,Young Bo Lee,Jong Chel Jeong,Seunghwan Lee,Hae Chul Park 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
DNA barcoding is a strong species identification tool for all animal taxa, and can easily be conducted when materials are under DNA friendly conditions. In contract, a full-length (659 bp) sequencing has been limited for the degraded DNAs extracted from old museum specimens. The initial challenges to retrieve the authentic DNA fragments from old museum specimens were attempted by obtaining short sequences (<300 bp) with the cloning process after PCR, making it both expensive and time-consuming. In this study, we employed a modified method to analyze the full-length DNA barcoding regions in 31~52 year-old butterfly specimens (301 dried specimens of 39 species) using direct sequencing after PCR with two different methods: 1) the successful PCR rates of 0 to 5.6% using four universal primer sets were too low to obtain authentic sequences and the cross-contamination was detected in almost all successful amplicons; 2) the success rates of PCR using specie-specific overlapping primer sets were distinctly high, reaching up to 75% with 98% authentic and 2% non-specific sequences. Thus, the result showed the method that using species-specific primer set per species yields the most effective success rates of both PCR and sequencing from degraded DNA without incorrect sequences.
( Taeman Han ),( Soojeong An ),( Seung-hyun Kim ),( In Gyun Park ),( Haechul Park ) 한국잠사학회 2016 International Journal of Industrial Entomology Vol.33 No.2
Sciaria thoracica Matsumura belonging to the family Sciaridae is recorded from Korea for the first time. This species is a conspicuous species having the rufous thorax in Palearctic region. The morphological diagnosis and photos of adult, and the result of DNA barcoding inferred from the Korean and the Japanese populations of S. thoracica are provided.
Retrieving Authentic DNA Barcoding Sequence from Old Insect Specimens
Taeman Han,Tae Hwa Kang,Oh Chang Kwon,Young Bo Lee,Mi Ae Kim,Hae Chul Park 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10
In DNA barcoding, the DNA degradation of old museum specimens has been limited full-length (658bp) sequencing. The challenges associated with the retrieval and authentication of degraded DNA extracts from fossil and old museum specimens were principally limited to analyze the relatively short sequences (<300 bp). Furthermore, almost protocols in other to analyzed the degraded DNA contained the cloning process after PCR causing the time-consuming and the rising costs. To overcome these problematic circumstances, we tried a modified method to analyze full-length of DNA barcoding region in 30~60 year-old butterfly specimens (225 samples in 28 species), using direct sequencing after PCR with species-specific overlapping primer sets per each species. As a result, all of 28 species have been successfully analyzed, although 178 samples (79%) are completely generated barcoding sequences ranged from 640 to 658 bp and 47 samples (21%) are partially sequenced ranged from 100 to 500 bp. Thus, the result showed that the direct PCR sequencing using the overlapping primer sets per species appears to have great potential efficiency for analysis of degraded DNA without incorrect sequences.
Han, Taeman,Lee, Hodan,Lee, Hae Ju,Park, Hae Chul,Lee, Seunghwan,Lee, Young Bo Blackwell Publishing Asia 2012 Entomological Research Vol.42 No.4
<P><B>Abstract</B></P><P>We reviewed the Korean species belonging to the tribe Physorhinini of the subfamily Elaterinae. <I>Anchastelater shaanxiensis</I> Schimmel, 2007 is recorded for the first time in Korea. We provide diagnosis criteria for the species of the tribe Physorhinini and of the genus <I>Anchastelater</I>, as well as a description, and illustrations of the female <I>A. shaanxiensis</I>. The first Korean record of Physorhinini based on the male <I>Anchastus aquilis</I>[!] Candèze, 1873 was found to be a misidentification of <I>Neopsephus</I> sp. in the tribe Dicrepidiini and it is therefore proposed to exclude from the Korean fauna.</P>
Taeman Han,Haechul Park,Youngbo Lee,In Gyun Park,Jongchel Jeong,Seunghwan Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
The previous studies for phylogenetic relationships within Elateridae were carried out, but not constructed a reliable evolutionary hypothesis. This study attempted to establish a robust evolutionary hypothesis, focusing on major subfamilies of the family Elateridae sensu stricto, using extensively selected 12 genetic markers, COI, 16S rRNA, 18S rRNA, 28S rRNA and 8 nuclear genes. As the results, phylogenetic analyses for 12 multiple genes constructed robust phylogeny with almost very strongly supported nodal values (>90%) and represented that the previously questioned systematic positions of nine subfamilies are fully resolved, excepting the basal lineage split. Especially, three subfamilies, which were recently reduced into tribal rank, Hypnoidinae, Oxynopterinae, and Denticollinae are monophyletic, respectively, and it is supported the traditional taxonomic schemes that had been treated these three taxa in subfamily rank. Whereas, Elaterinae, Hiopinae, and Melanotinae are clustered to a monophyletic group. Two tribes, Denticollini and Ctenicerini in Denticollinae are paraphyletic, respectively and it needs to reclassify their systematic positions. This study recovering of relationships between subfamilies using 12 gene loci resulted that their phylogenetic relationships are sufficiently and successfully resolved with strong supported nodes and provided more possible interpretations from subfamily to tribal levels than the previous studies.
A taxonomic review of the genus Acteniceromorphus Kishii, 1955 (Coleoptera; Elateridae) in Korea
Han, Taeman,Park, In Gyun,Park, Haechul Korean Society of Sericultural Science 2015 International Journal of Industrial Entomology Vol.31 No.2
The genus Acteniceromorphus is taxonomically reviewed for the first time in Korea. From the previously recorded three species, two species, A. selectus (Candèze, 1894) and A. fulvipennis <xref>(Lewis, 1894)</xref> are confirmed as misidientification of species which are endemic to Japan. Another species, A. chlamydatus <xref>(Lewis, 1894)</xref> is unavailable any Korean specimens. Additionally, we found A. ferrugineipennis <xref>(Candèze, 1879)</xref> as new to Korea. We also provide a comparison of DNA barcoding for two species previously misidentified and the newly recorded species, except A. chlamydatus. DNA barcoding result shows that each species is clearly delimited at species-level from each other by large interspecific genetic distance over 7.2%.
A New Species of the Genus Neopsephus Kishii 1990 (Coleoptera: Elateridae: Elaterinae) from Korea
Taeman Han,Jong Chel Jeong,Young Bo Lee,Seunghwan Lee,Hae Chul Park 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
We recognize a new species, belonging to the genus Neopsephus Kishii 1990, from Is. Jeju. The novel species is closely allied to N. takasago Kishii 1990 known from Taiwan. This study provides the detailed morphological characteristics investigated by SEM and molecular data inferred from COI and 16S rRNA of mitochondria.
Is a Natural Monument, Chrysochroa fugidissima (Coleoptera; Buprestridae) a Novel or Not?
Taeman HAN,Taehwa KANG,Yongbo LEE,Jongchel JEONG,Hyojung CHUNG,Seunghwan LEE,Ki-Gyoung KIM,Haechul PARK 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
Chrysochroa fulgidissima is the most beautiful Korean beetle and valuable insect in traditional culture because of ancient fulgidissima's adornments in Korea and Japan. The species is also recognized as very rare in the Korean fauna. However, we recently realized that the Korean population has been applied to Ch. fulgidissima without detailed taxonomic approaches since Japanese colonial rule of Korea. Therefore, we investigated 76 dried specimens belonging to seven populations collected from N. Vietnam, E. China, Taiwan, Japan, and Korea through morphological and molecular taxonomy using DNA barcoding of COI and 16S rRNA. As the result, the Korean population is strongly formed a monophyletic branch divided from other populations by genetic divergence of each 10.2%~11.7% in DNA barcoding and 6.4%~7.9% in 16S rRNA, and as well as fine structure in the male genitalia. It is allowed to us that the Korean population, which has been underestimated at species discrimination in morphology, may be considered as a novel species.