Aspergillus 속 미생물에 의한 발효비지의 항비만 효과

이상일(Lee, Sang-Il),이예경(Lee, Ye-Kyung),김순동(Kim, Soon-Dong),임종환(Lim, Jong-Hwan),서주원(Seo, Ju-Won),이인애(Lee, In-Ae) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.11

고지방식이로 비만을 유도한 ICR 생쥐를 동물 모델로 하여 7종의 Aspergillus속 미생물로 발효시킨 발효비지 의 항비만 효과를 조사하였다. 수컷 ICR 생쥐에 5주간 고지방 식이를 실시하여 비만을 유도하고 고지방식이에 발효 비지 2%를 첨가하여 6주 동안 사육하였으며, 체중, 섭취량, 혈청 총콜레스테롤, 중성지질, Alanine transaminase과 high-density lipoprotein cholesterol, 간성 glutathione과 lipid peroxide를 측정하였다. 체중의 경우에 AE4 발효 비지를 식이한 군에서 가장 많이 감소 (31.33%, P<0.05)하였고 간성 GSH와 ALT증가로 고지방혈증이 저해되었다. 이상의 결 과로부터 Aspergillus속 미생물로 발효시킨 발효비지는 항비만 효과가 있는 것으로 나타났으며, 나아가 인체에서도 유 의한 비만개선 작용을 기대할 수 있을 것으로 사료된다. The anti-obesity effect of soybean curd residues (biji) fermented by seven Aspergillus spp. was investigated with obese ICR mice fed a high-fat diet. After inducing obesity by feeding high-fat diet for 5 weeks, animals were fed with a high fat diet supplemented with 2% fermented soybean curd residues for 6 weeks. Total cholesterol, triglyceride, Alanine transaminase, high-density lipoprotein cholesterol, hepatic content of glutathione and lipid peroxide were determined. In the case of body weight, AE4 group showed most prominent decrease (31.33%, P<0.05) and increase of hepatic GSH and ALT demonstrated hyperlipidemia inhibition. From the results, it is concluded that soybean curd residues fermented by Aspergillus spp. has anti-obesity effect and it is thought that fermented soybean curd resides can reduce obesity in human significantly.

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

배추의 배축절편으로부터 캘러스와 뿌리 발생을 통한 안정적 형질전환

조미애,김춘해,민성란,고석민,유장렬,최필선,Cho, Mi-Ae,Kim, Choon-Ae,Min, Sung-Ran,Ko, Suck-Min,Liu, Jang-Ryol,Choi, Pil-Son 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.2

'정상' 배추의 배축절편을 선발마커로서 paromomycin 저항 성유전자를 갖고 있는 pPTN290으로 각각 형질전환된 EHA101, LBA4404, GV3101균주와 공동배양한 후 갤러스유도배지에서 형질전환캘러스를 얻은 후, 뿌리유도배지에서 부정근을 그리고 신초유도배지에서 신초를 각각 순차적으로 유도하였다. 형질전환캘러스를 얻은 후, 뿌리유도배지에서 부정근을 그리고 신초유도배지에서 신초를 가각 순차적으로 유도하였다. 형질전환캘러스 형성은 Agrobacterium균주에 따라 차이가 있었으며, 특히 EHA101균주에 공동배양된 배축절편으로부터 최대 6.1%까지 얻어졌다. 또한 각각의 형질전환캘러스 클론으로부터 형질전환 부정근과 신초 발생은 EHA101균주에서 60.7%와 38.2%, LBA4404에서 8.3%와 0%, GV3101에서 20.5%와 85.7%까지 각각 얻을 수 있었다. 형질전환식물체는 특별한 형태적 이상 없이 온실에서 정상적으로 자라 $T_{2}$종자를 얻을 수 있었다. GUS방법으로 7개의 후대 유식물체를 분석한 결과 gus유전자가 안정적으로 발현하고 있음을 확인하였고, 배추 genome에 single 또는 multiple copy로 전달되고 있음을 추측할 수 있었다. Hypocotyl explants of Chinese cabbage (cvs. "Jeong Sang") produced transgenic calli on callus induction medium (MS salt, B5 vitamin, 5 mg/L acetosyringone, 1 mg/L 2,4-D, 3% sucrose, 400 mg/L cefotaxime, 100 mg/L paromomycin, pH 5.8) after cocultivation with strains of Agrobacterium tumefaciens (EHA101, LBA4404, GV3101) harboring the pPTN290 containing paromomycin-resistance gene as a selectable marker, and then they transferred to root induction medium (1/2MS salt, MS vitamins, 2% sucrose, 100 mg/L paromomycin, 100 mg/L cefotaxime, pH 5.8) and shoot induction medium (MS salt, B5 vitamin, 4 mg/L $AgNO_3$, 4 mg/L 6-benzyladenine, 3 mg/L alpha-naphthaleneacetic acid, 100 mg/L paromomycin, 100 mg/L cefotaxime, 3% sucrose, pH 5.8) in order. There was a significant difference in the frequency of transgenic calli depending on Agrobacterium strains. In particular, the highest frequency (6.1%) of transgenic calli was obtained from the hypocotyls cocultivated with EHA101 strains. Also, the frequency (%) of transgenic root and plants from each transgenic callus clone were obtained with 60.7% and 38.2% in EHA101, with 8.3% and 0% in LBA4404, with 20.5% and 85.7% in GV3101 strains, respectively. They were grown to maturity in a greenhouse and normally produced $T_2$ seeds. GUS histochemical assay for progeny ($T_2$) revealed that the transgenes was expressed in the plant genome, and progeny analysis from 7 independent transgenic events demonstrated that the transformants transmitted the transgene as a single or multiple functional locus.

Chronic Hepatitis B Infection Is Significantly Associated with Chronic Kidney Disease: a Population-based, Matched Case-control Study

Kim, Sung-Eun,Jang, Eun Sun,Ki, Moran,Gwak, Geum-Youn,Kim, Kyung-Ah,Kim, Gi-Ae,Kim, Do Young,Kim, Dong Joon,Kim, Man Woo,Kim, Yun Soo,Kim, Young Seok,Kim, In Hee,Kim, Chang Wook,Kim, Ho Dong,Kim, Hyun The Korean Academy of Medical Sciences 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.42

<P><B>Background</B></P><P>Hepatitis B virus (HBV) infection leads to hepatic and extrahepatic manifestations including chronic kidney disease (CKD). However, the association between HBV and CKD is not clear. This study investigated the association between chronic HBV infection and CKD in a nationwide multicenter study.</P><P><B>Methods</B></P><P>A total of 265,086 subjects who underwent health-check examinations in 33 hospitals from January 2015 to December 2015 were enrolled. HBV surface antigen (HBsAg) positive cases (n = 10,048), and age- and gender-matched HBsAg negative controls (n = 40,192) were identified. CKD was defined as a glomerular filtration rate (GFR) < 60 mL/min/1.73 m<SUP>2</SUP> or proteinuria as at least grade 2+ of urine protein.</P><P><B>Results</B></P><P>HBsAg positive cases showed a significantly higher prevalence of GFR < 60 mL/min/1.73 m<SUP>2</SUP> (3.3%), and proteinuria (18.9%) than that of the controls (2.6%, <I>P</I> < 0.001, and 14.1%, <I>P</I> < 0.001, respectively). In the multivariate analysis, HBsAg positivity was an independent factor associated with GFR < 60 mL/min/1.73 m<SUP>2</SUP> along with age, blood levels of albumin, bilirubin, anemia, and hemoglobin A1c (HbA1c). Likewise, HBsAg positivity was an independent factor for proteinuria along with age, male, blood levels of bilirubin, protein, albumin, and HbA1c. A subgroup analysis showed that HBsAg positive men but not women had a significantly increased risk for GFR < 60 mL/min/1.73 m<SUP>2</SUP>.</P><P><B>Conclusion</B></P><P>Chronic HBV infection was significantly associated with a GFR < 60 mL/min/1.73 m<SUP>2</SUP> and proteinuria (≥ 2+). Therefore, clinical concern about CKD in chronic HBV infected patients, especially in male, is warranted.</P>

Hepatitis B Virus Infection Is Significantly Associated with Chronic Kidney Disease: A Population-Based, Matched Case-Control Study in Korea

( Sung Eun Kim ),( Eun Sun Jang ),( Moran Ki ),( Geum-youn Gwak ),( Kyung-ah Kim ),( Gi-ae Kim ),( Do Young Kim ),( Dong Joon Kim ),( Man Woo Kim ),( Yun Soo Kim ),( Young Seok Kim ),( In Hee Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatitis B virus (HBV) infection leads to hepatic and extrahepatic manifestations including chronic kidney disease (CKD). However, the association between HBV and CKD is not clear. This study aimed to investigate the association between CKD and HBV infection in a population-based, nationwide multicenter study. Methods: A total of 268,422 subjects who underwent health-check examination in nationwide 33 hospitals from Jan 2015 to Dec 2015, were enrolled. We identified 10,473 adults who had chronic HBV infection by hepatitis B surface antigen (HBsAg) positivity (cases), and 41,892 age-and sex-matched HBsAg negative people (controls) in the same dataset. CKD is defined as GFR<60ml/min/1.73² (GFR categories G3a-G5), according to KDIGO 2010 clinical practice guideline. Proteinuria was defined as the presence of urine protein of at least grade 2+. Results: HBsAg positive cases showed significantly higher frequency of CKD (3.3%) than in controls (2.7%) (P=0.006). Also, the prevalence of proteinuria in HBV cases (18.4%) was significantly higher than in controls (13.9%) (P<0.001). Multivariable logistic regression analysis for associated factors of CKD revealed that age, serum protein, serum albumin, hemoglobin, hemoglobinA1c and HBsAg positivity were independent predictors (P<0.05). Also, age, sex, HBsAg positivity, serum protein, hemoglobin, hemoglobinA1c and platelet were independent predictors of the proteinuria (P <0.05). Conclusions: HBV infection was significantly associated with GFR<60 ml/min/1.73² and proteinuria (≥2+). Therefore, in the era of effective antiviral therapy, clinical concern on the extrahepatic manifestations including kidney disease is warranted.

Hepatitis C Virus Infection Is Associated with Lower Levels of Serum Cholesterol, Triglyceride, and LDL-Cholesterol Level: A Population-Based, Matched Case-Control Study in Korea

( Sung Eun Kim ),( Eun Sun Jang ),( Moran Ki ),( Geum-Youn Gwak ),( Kyung-Ah Kim ),( Gi-Ae Kim ),( Do Young Kim ),( Dong Joon Kim ),( Man Woo Kim ),( Yun Soo Kim ),( Young Seok Kim ),( In Hee Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatitis C virus (HCV) entry and replication is associated with interruption of lipid metabolism. We aimed to investigate the association between anti-HCV positivity and serum lipid profiles in a nationwide, multicenter study. Methods: A total of 268,422 subjects who underwent health-check examination in nationwide 33 hospitals from Jan 2015 to Dec 2015 were enrolled. Data on the anti-HCV positivity, and biochemical laboratory results including serum levels of total cholesterol, triglyceride, LDL-cholesterol, and HDL-cholesterol were obtained and analyzed. Among the subjects, 1,360 anti-HCV antibody positive cases (0.6%), and 5,440 anti-HCV negative controls matched for age and sex to the cases were identified in same dataset. Results: Though body mass index (BMI) was not different between case and control group, the mean serum levels of total cholesterol (185 mg/dL), triglyceride (111.8 mg/dL), LDL cholesterol (117.4 mg/dL), and HDL cholesterol (54.5 mg/dL) were all significantly lower in anti-HCV positive group than in controls (192.5, 119.1, 125.1, and 55.8 mg/dL, respectively). By multivariable analyses using logistic regression, the independent factors associated with elevated serum total cholesterol (≥200 mg/dL) were age, male sex, anti-HCV positivity or HCV-RNA positivity. In addition, the independent factors associated with elevated serum triglyceride(≥150 mg/dL) were age, male sex, BMI (≥25kg/m2) HbA1C (≥5.5%), ALT (≥40IU/L), anti-HCV positivity or HCV-RNA positivity. Moreover, the independent factors associated with elevated LDL-cholesterol (≥130 mg/dL) were age, male sex, and anti-HCV positivity or HCV-RNA positivity. Conclusions: In this large population-based data, HCV infection independently associated with lower serum total cholesterol level, lower triglyceride level and lower LDL cholesterol level.

Comparing the Writings and Attitudes of High School EFL Students by Proficiency Levels

Sung-Ae Kim 한국영어교과교육학회 2019 영어교과교육 Vol.18 No.4

This study compares the writings and attitudes of Korean high school students by proficiency levels. The participants were 100 second-year students from a high school located in a metropolitan city. A source text was used to facilitate the participants’ writing. Considering the possible effect of the topic, the participants were divided into two groups (Text A & Text B groups), and each group read a source text on a different topic. To examine the effect of English proficiency on writing, each text group was divided into two sub-groups based on their performance in the National English Ability Test. A questionnaire survey was also conducted after the writing task to examine each proficiency group’s attitudes toward writing. Analysis of the writing samples used CohMetrix 3.0, focusing on lexical proficiency and syntactic complexity with six indices of writing. According to the result, the two proficiency groups using Text A showed a significant difference only in NW, whereas those using Text B showed a significant difference in NW/S and LE as well as NW. The survey result showed that the two proficiency groups shared common attitudes toward writing, with some differences only in the degree. Based on the result, the study recommends some strategies for writing instruction in high school English classes.

Malignant Adenmyoepithelioma of the Breast

Sung-Woo Jang,Zisun Kim,Jung Cheol Kuk,Jaehong Jeong,Sung Mo Hur,Gui Ae Jeong,Jun Chul Chung,Gyu Seok Cho,Eung Jin Shin,Hyung Chul Kim,Cheol Wan Lim 대한종양외과학회 2016 대한임상종양학회 학술대회지 Vol.2016 No.2

A study on microbiological ecology in beef from production to distribution through 16S rRNA gene sequencing

Sang Ah Kim,Jiwon Park,Jeongyeon Yeom,Dongryeoul Bae,Sun Ae Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

The antioxidant and chemopreventive potentialities of Mosidae (Adenophora remotiflora) leaves

Ae-Jung Kim,Myung-Ryun Han,Myung-Hwan Kim,Myoungsook Lee,Taek-Joon Yoon,Sang-Do Ha 한국영양학회 2010 Nutrition Research and Practice Vol.4 No.1

Our study focused on the antioxidant activities of Mosidae leaf ethanol extract (MLE) and included measurements of reducing power, total phenolic compounds, DPPH radical scavenging activity, and hydroxyl radical scavenging activity. In order to determine whether or not MLE evidences any chemopreventive activities, experimental lung metastasis was induced via the i.v. inoculation of colon26-M3.1 carcinoma cells into BALB/c mice. Additionally, we attempted to characterize any possible cytotoxic effects in murine normal splenocytes and tumor cells (B16-BL6 and colon26-M3.1). The total phenolic content and reducing capacity were measured at 39 ㎎/100 ㎖ and 1.24, respectively, whereas the DPPH and hydroxyl radical scavenging activities of MLE were measured to be 88.89% and 22.10%, respectively. Prophylactic i.v. treatment with MLE resulted in a dose-dependent and significant inhibition of lung metastasis. Specifically, a MLE dose of 200 ㎍ per mouse resulted in an 88.90% inhibition of lung metastasis. For the cytotoxicity assay, MLE doses up to 100 ㎍/㎖ were not shown to affect the growth of normal murine splenocytes. Additionally, the survival of normal cells was not affected at MLE doses below 500 ㎍/㎖. However, MLE doses up to 500 ㎍/㎖ reduced the percentage of tumor cell growth for B16BL6 (67% alive) and colon26-M3.1 (62% alive) cells.