폐슬러지 감량화 및 재활용을 위한 오존 처리시 하수슬러지내의 미생물 군집구조의 변화

홍준석,임병란,안규홍,맹승규 대한상하수도학회 2004 상하수도학회지 Vol.18 No.1

The change of the microbial community structure in excess sludge of different sewage treatment plants by ozone treatment was investigated by quinone profiles. The resulting ozone dosage ranged from 0.1 to 0.4 gO₃/gTS. In terms of overall sludge reduction, more than 50% reduction of the total sludge mass could be achieved by ozone treatment at 0.4 gO₃/gTS. Quinone concentration and type in sludge of different treatment plants were remarkably decreases with increasing ozone dose. Ubiquinones(UQs)-8, -10 and MK-8 were still remained in the ozonized sludge at 0.4 gO₃/gTS. The results of this study showed that the remaining microorganisms belong to UQs-8, -10 and MK-8 were difficult to destruct cell membrane or wall by ozonation. Fecal Streptococci and Salmonella were not detected at ozone dose of 0.2 gO₃/gTS, but Fecal Colifom was not detected at ozone dose of 0.4 gO₃/gTS.

몇가지 β-lactam 항생제의 약효평가를 위한 Minimum Inhibitory Concentration(MIC)의 비교

洪淳德,金鐘國,李東善,河相哲,李相瀚,李載蘭,安賢淑 慶北大學校 1991 論文集 Vol.51 No.-

In order to screen the new β-lactam antibiotics for clinical use. we were compared MIC values of various β-lactam antibiotics. We also tested the influence of pH, inoculum size, stability of antibiotics in medium and some kinds of media on MIC value. As a result, cefotaxime, cefoperazone, piperacillin, cefamandole, cephaloridine, cephapirin, cefazolin, cephalothin, cephaioylycn were scored in antimicrobial activity of MIC data.

種球의 低溫處理 및 培地의 組成이 마늘의 캘러스增殖 및 幼植物體 分化에 미치는 영향

閔成蘭,李殷模,羅相煜,盧泰弘,李永馥 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Effects of low temperature treatment and medium composition on callus proliferation and shoot differentiation of garlic seed bulbs were investigated. The calli derived from shoot-tip explants of bulbs which had been stored at 4℃ for 2 months were proliferated 1.9 times more vigorously by fresh weight on callus induction medium and regenerated 11 times more efficiently in number on shoot differentiation medium supplemented with 2 ㎎/L kinetin and 0.1 ㎎/L NAA than the calli from those at room temperature. Modified MS media containing KNO_3 and (NH_4)_2SO_4 of 40 mM each or containing KNO_3, (NH_4)_2SO_4, and KCI of 10 mM each were more effective than the original MS in callus proliferation and shoot differentiation. We believe that this culture system can be used for the mass production of virus-free stocks garlic.

Adriamycin 투여가 흰쥐 상아질 형성에 미치는 영향에 관한 연구

이상열,김선헌,최홍란,이은주 전남대학교 치과대학 1992 전남치대논문집 Vol.4 No.1

In order to study the toxic effect of adriamycin on the formation of dentin of the rat, adriamycin(5㎎/㎏ body weight) was injected intravenously, and animals were sacrificed at 1, 3, and 10 days after administration. In the light micrographic observation, aggregation of densely stained cells were observed along the wall of the pulp canal and the odontoblastic layer disappeared after 1 and 3 days. The predentin was not even thickness and irregular in boundary compared to normal predentin. The formation of osteodentin, which has cellular inclusions in the matrix, was noted after 10 days. Electron microscopic observation revealed that the cells in aggregation had long slender cytoplasmic processes and contained more profiles of endoplasmic reticulum and secretory granules. The odontoblasts showed degenerative changes such as cytoplasmic vacuolation and fragmentation of rEr. The cells in the osteodentin and on the surface of irregular osteodentin contained dilated cisternae of rEr and secretory granules. Some cells showed necrotic and degenerative changes. There were no odontoblastic processes and dentinal tubules in the osteodentin matrix, central region of which was densely packed with collagen fibers. It therefore appears that osteodentin formation was observed in the pulp of root canal after adriamycin administration and it is the result of an abnormal differentiation of pulp mesenchymal cells.

급성 림프구성 백혈병 환자에서 발생한 침습성 국균 심내막염 2예

신성철,백경란,정재홍,송봉근,김동희,전경만,채제욱,손준성,정숙인,오원섭,송재훈 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

국균은 호흡기를 통해 유입되며 심한 면역기능저하 환자에서 기회감염을 일으키는 진균이며 주로 혈관을 따라 폐를 침범하고 심장과 골침범은 매우 드문 것으로 알려져 있다. 저자들은 급성 림프구성 백혈병 환자에서 발생한 침습성 국균 심내막염을 2예을 경험하였다. 19세 남자환자로 동종 조혈모세포이식 후 발생한 고열과 요통의 원인으로 감염성 심내막염 진단하에 수술결과 국균에 의한 감염으로 확증되어 수술적 절제술과 liposomal amphotericin B로 치료하였으나 국균 심내막염의 재발과 국균 척추염의 악화, 뇌 출혈로 사망하였다. 23세 남자환자로 관해요법 후 발생한 고열과 요통의 원인으로 국균 심내막염과 국균 척추염 진단하에 수술적 절제술과 amphotericin B로 치료하여 1년 2개월 동안 국균 심내막염의 재발은 없었으나 지속적인 백혈병의 재발과 국균 척추염의 악화, 침습성 폐 국균증으로 사망하였다. Although Aspergillus endocarditis has rarely been reported, it can cause fatal complications in hematologic malignancy patients and allogeneic stem cell transplant recipients. We experienced two cases of aspergillus endocarditis developed in acute lymphoblastic leukemia patients. Case; A 19-year-old patient developed Aspergillus endocarditis after allogenic hemopoietic stem cell transplantation. He was treated with surgical intervention and liposomal amphotericin B. He died of recurred Aspergillus endocarditis and cerebral hemorrhage probably related with aspergillosis of central nervous system. Case 2; A 23-year-old patient developed invasive Aspergillus endocarditis after induction chemotherapy. Aspergillus endocarditis was successfully treated by surgical intervention and amphotericin B. He died of refractory neutropenic fever and sepsis after the third relapse of leukemia and repetitive chemotherapy. He probably had invasive pulmonary aspergillosis without evidence of endocarditis recurrence. Because the mortality of Aspergillus endocarditis is very high, early diagnosis and surgical intervention are very important for better outcome.

일부 부인과 양성 질환자의 지방조직 및 혈청의 PCBs와 DDE 농도

박성균,이강숙,노영만,구정완,민선영,한진구,고원경,김미란,정재근,이종승,김진홍 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 연구는 지방조직과 혈청의 PCBs와 DDE 농도를 측정하고, 이에 영향을 미치는 것으로 알려진 요인들과의 관련성을 평가하고자 하였다. 방법 : 유방암, 자궁내막증 등 유기염소계 화합물의 영향이 있는 것으로 알려진 호르몬 관련 질병을 제외한 부인과 환자 52명을 대상으로 지방조직과 혈청을 분석하였으며, 설문조사를 통하여 연령, 교육수준, 직업, 비만도, 흡연과 음주, 출산력, 수유력등을 조사하였다. 지방조직과 혈청의 PCBs와 DDE 농도는 가스크로마토그래피를 사용하여 측정하였다. 결과 : 지방조직의 경우, 13개 PCBs congeners 농도의 합은 중앙값이 48.29 ng/g, 기하평균값이 56.78 ng/g이었고, DDE 농도는 중앙값이 142.89ng/g, 기하평균값이 117.06 ng/g이었으며, 혈청의 경우는 PCBs congeners 농도의 합은 중앙값이 4.67 ㎍/L, 기하평균값이 4.85 ㎍/L이었고, DDE 농도는 중앙값이 1.75 ㎍/L, 기하평균값이 2.09 ㎍/L로 나타났다. 지방조직과 혈청의 DDE 농도 사이는 높은 상관성을 보였으나(r²=0.310, p=0,0002), PCBs는 상관성이 약한 것으로 나타났다(r²=0.029, p=0.2582). 영향요인을 범주화한 후 로그변환 시킨 지방조직과 혈청의 PCBs, DDE 평균의 차이를 분석한 결과, 지방조직의 DDE만이 출산자녀수(p=0.015), 초산연령 (p=0.014), 비만지수(p=0.035)와 연관성이 발견되었다 다중회귀분석의 결과 지방조직의 DDE는 출산자녀수가 적을수록, 초산연령이 늦을수록 증가하는 경향을 보였다. 결론 : 출산자녀수와 초산연령, 비만도가 지방조직의 DDE와 관련성을 보였으며, 또한 유기염소계 화합물의 장기간의 노출에 의한 영향을 평가하는데 있어서 혈청보다 지방조직이 더 유용한 생물지표임을 알 수 있었다. Objectives : This study was purposed to determine the levels of PCBs and DDE in adipose tissue and serum and to evaluate the relations with factors affecting these levels. Methods : We analyzed adipose tissues and sera from 52 gynecologic benign disease patients aged 27-78 years, except hormonal diseases such as breast cancer and endometriosis. We also surveyed age, education, occupation, body mass index (BMI), smoking, drinking, number of children, and duration of breastfeeding by questionnaires. Tissue and serum levels of PCBs and DDE were measured using gas chromatography. Results : The median and geometric mean levels of PCBs were 48.29 ng/g, 56.78 ng/g for adipose tissue and 4.67 ㎍/L, 4.85 ㎍/L for serum, and those of DDE were 142.89 ng/g, 117.06 ng/g for adipose tissue and 1.75 ㎍/L, 2.09 ㎍/L for serum, respectively. Adipose tissue and serum levels for DDE showed high correlation (r²=0.310, p=0.0002), but those of PCBs didn't (r²=0.029, p=0.2582). In analyses of the differences of the means of log transformed adipose tissue and serum PCBs and DDE levels for groups of potential covariates, only adipose tissue DDE levels were significantly associated with number of children (p=0.015), age at first birth (p=0.074) and BMI (p=0.035). In multiple regression analysis, adipose tissue DDE levels were significantly increased with decreasing number of children and increasing age at first birth. Conclusions : This study suggested that parity and adiposity were associated with levels of DDE in adipose tissue, and adipose tissue is a better biomarker than serum for evaluating the long-term exposure of organochlorines.

고삼투압성 비케톤성 상태의 예후인자

안성훈,김양원,김미란,진헌철,안지영,이상래,류석용,김홍용,김성준,이병권,김경환 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Background: A hyperosmolar nonketotic state has been known to have a high mortality, and even now, despite this high mortality, only a few studies of this disease have been performed. We studied the prognostic factors for the hyperosmolar nonketotic state. Methods: We retrospectively studied the cases of 40 patients who were in a hypersomolar nonketotic state when admitted to Sanggye Paik Hospital during the 6-year Period from 1995 through 2000. We divided the hyperosmolar nonketotic patients into two groups, the complete recovery group and the incomplete recovery group, and compared the clinical features, the laboratory findings, and the precipitating factors between two groups. Results: 1) A total of 40 patients were studied: 24 in the complete recovery group and 16 patients in the incomplete recovery group. The mortality rate was 32.5%. 2) No significant statistical difference existed among the clinical features of the two groups, except for the sex(p<0.01). 3) Among the laboratory findings of both groups, analysis revealed that the effective osmolarity was significantly higher among those in the incomplete recovery group(p<0.01). Serum sodium concentration was also significantly higher among those in the incomplete recovery group(p<0.01). Serum creatinine was also significantly higher among those in the incomplete recovery group(p<0.05). Serum bicarbonate concentration, on the other hand, was significantly lower among those in that group(p<0.05).4) Infection was identified as the most common precipitating factor(62.5%). Among the precipitating factors of the two groups, there were significant statistical difference in pneumonia, UTI, and inappropriate glucose control. 5) A significant statistical difference existed among the initial level of consciousness of both groups(p<0.05). 6) The only significant independent factor responsible for prognosis of nonketotic hyperosmolar state patients was the sex. Conclusion: The sex was only significant independent prognostic factor of nonketotic hyperosmolar state patients.

운동장애형 기능성 소화불량증 환자에서의 식도내압검사 성적

황일란,김진홍,이광재,김정민,함기백,조성원 대한소화관운동학회 1998 Journal of Neurogastroenterology and Motility (JNM Vol.4 No.1

급성 심근경색증 진단을 위한 Triage® Cardiac System의 진단적 유용성 : Comparison with Established Devices

이상래,김미란,노준영,류석용,김홍용,김성준 대한응급의학회 2002 대한응급의학회지 Vol.13 No.4

Purpose: The purpose of this study is to evaluate the diagnostic value of the Triage® Cardiac System (Biosite, USA) for quantitative detection of acute myocardial infarction. Methods: During a three-month period, we prospectively studied the cases of patients who visited the emergency department (ED) with suspicious acute ischemic chest pain. With the exclusion of patients with stable angina (according to ACC/AHA guideline) and non-cardiogenic chest pain, a total of 20 patients were studied. We evaluated the sensitivity and the specificity of the Triage® Cardiac System, and we compared the results with those from an established device (Elecsys® 1010, Boehringer Mannheim, Germany) by using a Pearson correlation and the student t-test. Results: The mean operating time of the Triage® Cardiac System was faster than that of the established device (16±1.2 min vs 30±0.6 min). The sensitivity and the specificity were same for the two devices. The sensitivity and the specificity of CK-MB were 63.6% and 88.9% respectively, and those of cardiac troponin were 54.5% and 100%. In the evaluation of patients who arrived at the ED after 4 hours of symptom onset, the sensitivity and the specificity were high. The Pearson correlation of CK-MB between the two devices was significant (r=0.970, p<0.01). The CK-MB values of acute myocardial infarction were significantly different with unstable angina (Triage® Cardiac System: t=2.857 p=0.017; Elecsys® 1010: t=2.804 p=0.018). Conclusion: The Triage® Cardiac System is a good diagnostic device for acute myocardial infarction, and its fast reporting of the result and its ease to use in the ED are superior to those of the established device.

암환자가 지각한 좋은 간호사의 특성

조남옥,홍여신,한성숙,엄영란 병원간호사회 2006 임상간호연구 Vol.11 No.2

This study was based on a phenomenological approach. All patients were hospitalized cancer patient who had insight about cancer. Data were collected through in-depth interviews during the months of July and August, 2004. Interviews lasted one and half hours on an average and data were analyzed by Van Kaam method. Result: Four essential attributes of good nurse were identified: compassion, need fullfillment, virtuous and professional expertism. And four essential attributes of bad nurse were identified: insincere, neglect, disregard and lack of expertism. Conclusion: The results of this study showsd that cancer patients perceived the good nurse as possessing the qualities of compassion for patients, having virtuous attitude and professional expertism and fullfilling patient's need. And cancer patients perceived the nuse showing familial intimacy as a good nurse. This perception of cancer patients was influenced by tradition and cultural background. So there is a need for further research about our own culture and its influences on nursing practice.