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Mutations in gyrA and gyrB among Drug Resistant Mycobacterium Tuberculosis in Korea
( Hee Joo Lee ),( Hwi-Jun Kim ),( Ryeun Heo ),( Cheon-Tae Kim ),( Hee-Jin Kim ),( Jeong-hui Gwon ),( Gicheon Bae ),( Sumi Kang ),( Soul-hee Kim ),( Seungmo Kim ),( Eunseon Kim ),( Arim Song ),( Dea-Se 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0
Purpose In 2020 KATRD, we analyzed 65 isolates to see how gyrA and gyrB are associated with 7H9 broth microdilution method (BMD) and Lowenstein-Jensen (L-J) drug susceptibility test (DST) because fluoroquinolones (FLQ) have been recognized as important anti-TB agents. In this study, the aim is to evaluate the correlation between gyrA and gyrB mutations and resistance to FLQ with BMD and L-J DST as a follow up of the previous study. Method Since 2020, we have analyzed 304 INH or RIF mono resistant cases by molecular DST using sequencing for gyrA and gyrB genes of FLQ. MICs were measured by BMD for moxifloxacin (MFX, 0.0625~8.0 μg/mL) and levofloxacin (LFX, 0.0625~8.0 μg/ mL). In L-J DST, concentration of MFX was 1.0 μg/mL, 2.0 μg/mL and LFX was 2.0 μg/mL. Results In gyrA and gyrB sequencing, 270 strains (88.81%) were wild type (WT), 34 strains (11.18%) were mutant type (MT). Among 29 gyrA MT strains, 13 isolates (44.82%) had mutation at D94G, 7 isolates (24.14%) at A90V and 5 isolates (17.24%) at D94A. Among 5 gyrB MT strains, 2 isolates (40%) had D500N mutation. In L-J DST, 100% of gyrA MT strains were resistant to MFX. On the other hand gyrB MT strains, 60% to MFX and 40% to LFX were resistant. In BMD, 93.10% of gyrA MT strains ranged 0.5 ~ 4.0 μg/mL and 60% of gyrB MT strains ranged 0.5 ~ 4.0 μg/mL to MFX. Meanwhile 96.55% of gyrA MT strains ranged 1.0 ~ 8.0 μg/mL and 80% of gyrB MT strains ranged 1.0 ~ 8.0 μg/mL to LFX. Conclusions Most of the mutant isolates had mutations in gyrA and most of mutant type (38.23%) was gyrA_D94G (GAC/GGC). In this study we observed gyrA genes were associated with higher MICs based on 7H9 and L-J DST than gyrB genes. # No.2021R1F1A1061358
Mutations in gyrA and gyrB Among Drug Resistant Mycobacterium Tuberculosis in Korea
( Seungmo Kim ),( Ryeun Heo ),( Hee Joo Lee ),( Cheon-Tae Kim ),( Hee-Jin Kim ),( Jong-Wook Hong ),( Soul-hee Kim ),( Tae-Shik Park ),( Dea-Seon Han ),( Hyeon-Su Kim ),( Jong-Myun Song ),( Mi-so Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.0
Background Fluoroquinolones have been recognized as important anti-TB agents based on new tuberculosis treatment guidelines. In this study we have evaluated gyrA and gyrB how to relate with MIC based on 7H9 and phenotypic DST Results based on Löwenstein-Jensen (L-J) medium. Method Since 13th July 2020, 108 cases have analyzed gyrA and gyrB genes for fluoroquinolones. We have tested bedaquiline (0.03125~4 μg/mL), delamanid (0.00625~0.8 μg/mL), moxifloxacin (0.0625~8.0 μg/mL), levofloxacin (0.0625~8.0 μg/mL), linezolid (0.0625~8.0 μg/mL) using 7H9 broth. In Löwenstein-Jensen (L-J)medium, low concentration of moxifloxacin was 1.0 μg/mL and high concentration of moxifloxacin was 2.0 μg/mL, And that of levofloxacin and linezolid was 2.0μg/mL. Results All of mono-INH resistant cases analyzed wild type in gyrA and gyrB genes. There were 7 mutant type analyzed in gyrA and 1 mutant type in gyrB gene among 54 RIF resistant and MDR cases. All of 8 cases what we analyzed mutant type in gyrA and gyrB genes was interpreted resistant in phenotypic DST using L-J medium. Additionally all of 52 cases interpreted susceptible in phenotypic DST using L-J medium was wild type in gyrA and gyrB genes. The distribution of MICs for moxifloxacin what analyzed mutant type in gyrA gene was middle and high level (1.0 ~ 4.0 μg/mL). And The MIC for moxifloxacin what analyzed mutant type in gyrB gene was middle level (0.5 μg/mL). The distribution of MICs for levofloxacin what analyzed mutant type in gyrA gene was high level (4.0 ~ 8.0 μg/mL). And The MIC for levofloxacin what analyzed mutant type in gyrB gene was middle level (1.0 μg/mL). Conclusions We found 8 mutant type cases in gyrA and gyrB genes. Most of mutant type (75%) was gyrA_D94G(GAC/GGC). We observed gyrA and gyrB genes were associated with MICs based on 7H9 and phenotypic DST Results based on L-J medium.
성장호르몬 분비성 뇌하수체 선종에서 소마토스타틴 수용체 (제2아형, 제5아형), G_i2α 및 Pit-1 유전자 발현
류미숙,양인명,박철영,우정택,김성운,김진우,김영설,최영길,김은희,박승준,김국기 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2
Background: Mutation of Gs protein subunit (gsp oncogene), detected in about 30∼40% of growth hormone (GH)-secreting pituitary tumors, is associated with an increased long-acting somatostatin analog octreotide sensitivity. However, the mRNA expression of somatostatin receptor (sst) was not changed in the GH-secreting pituitary tumor, regardless of whether they were gsp oncogene positive or negative. This suggests that the expression of genes coding for G_i2α, Pit-1 and the other factors involved in the regulation of secretory activity in somatotrophs is likely to be altered in gsp oncogene positive tumors. We observed the impact of the gsp oncogene on the expression of the genes coding for Gi2, Pit-1 and sst (2&5) in GH-secreting pituitary tumors. Methods: The GH response to octreotide was examined in 13 acromegalic patients before transsphenoidal adenomectomy. Genomic DNA and RNA were extracted from fresh frozen tumor tissues. PCR was performed to amplify and sequence the region between codon 184 and 251 that includes exons 8 and 9 of the Gs gene. Sst2, sst5, G_i2α and Pit-1 mRNA levels were measured by semi-quantitative RT-PCR. Results: Sst2 and sst5 mRNA transcripts were detected in all tumors (7 gsp +, 6 gsp-). The amount of sst transcripts varied considerably varied between the tumors. There were no significant differences in sex, age, tumor size, grade or basal GH levels. Pit-1 and sst2 mRNA levels were not different. In contrast, G_i2 mRNA levels were significantly higher in gsp (+) while sst5 mRNA levels were higher in gsp (-). Conclusion: These data suggests that gsp oncogene may increase Gi2α levels but decrease sst5 mRNA levels. However, Pit-1 and sst2 mRNA expression may not be affected by gsp oncogene. The increased expression of the G_i2α gene might be an inhibitory compensatory response to the action of gsp oncogene
Paeoniae radis alba mediated alleviation of atopic dermatitis like immune alterations in mice (II)
Yong Heo,Ji Youn Kim,Kyeong Uk Yeo,So Ryeon Hwang,ARam Kim,Ji Hyun Yu,Jae Hee Lee,Na Ri Seo,Ah Rang Jo,Ye Soul Jeon,So Jung Sin,Hyung Jun Kim,Byung Ki Song,Jong Suek Shin 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8