Interleukin-2가 Mouse 간실질세포의 미세구조에 미치는 영향

손승국,백태경,이규식,정호삼 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

It is well known that interleukin-2, a kind of lymphokine produced by the lymphocytes stimulated by antigen or mitogen, mediates antitumor effects by stimulating the proliferation of helper and cytotoxic T-cells, augmenting the tumorlytic activities of natural killer cell and promoting the differentiation of precusor T-cell into lymphokine activated killer cell in vivo. Thus interleukin-2 has been widely used as immunotherapeutic agent for various sarcoma and carcinoma and also regressed the metastasis of malignant tumors. Although interleukin-2 is beneficial to cancer patient, it also develops many toxic effects such as fever, chillness, general malaise, mental confusion, vomiting, diarrhea, hepatic and renal dysfunction, and capillary leakage syndrome, including the general edema, heavy weight gain, ascites any pulmonary edema, which limits clinical use of interleukin-2. The author undertook this experiment to pursue the effect of interleukin-2 on the ultrastructure of hepatocyte in mice. A total of 75 healthy male DDY mice, weighing about 20gm, were used as experimental animals. The experimental animals were divided into the control and interleukin-2 treated groups, and then interleukin-2 treated mice were subdivided into 6 hours, 12 hours, 24 hours and 48 hours groups by intervals of time after administration and each group allocated 15 mice. Mice of interleukin-2 treated group were administrated with 2 million unit of interleukin-2, per kg of body weight, diluted in intraperitoneally. Animals were sacrificed 6,12,24 and 48 hours after administration. The specimens, left anterior hepatic lobe, were prefixed in 2% glutaraldehyde-2,\.5% paraformaldehyde solution buffered by Millonig's phosphate buffer (pH 7.2) for 2-4 hours and postfixed in 1% osmium tetroxide buffered by same buffer solution for 2 hours at 4℃. After dehydration, the specimens were embedded in Epon 812 and the ultrathin sections, 600-800A inthickness, were made with LKB V ultramicrotome. The preparations were stained with uranyl acetate and lead citrate and observed with JEM 100cx-II electron microscope. The result obtained were as follows; 1. Dilation, sacculation and disruption of cisternae of rough endoplasmic reticulum accompanied with dissociation of membrane bound ribosome were observed but as time goes by, these changes were recovered. 2. Disorganization of mitochondria was observed. 3. Proliferations of smooth endoplasmic reticulum associated with depletion of glycogen particles was recognized. But as time goes by, the glycogen particles were represented. 4. Numerous variously sized autophagic vacupoles and multivesicular bodies were observed. 5. Numerous lipid droplets were observed but they were disappeared as time goes by. Based on the result obtained, it is suggested that interleukin-2 would induce changes of ultrastructures of hepatocyte in mouse but these changes were recovered as time goes by.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Analysis of Symptom Determinant of Cucumber mosaic virus RNA3 via Pseudorecombinant Virus in Zucchini Squash

Seung Kook Choi,Ju Yeon Yoon,Jang Kyung Choi,Kook-Hyung Kim,Seong Han Sohn 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.4

Role of the Anorectal Physiology in Anorectal Disorders

Sohn, Seung Kook 대한소화관운동학회 1994 Journal of Neurogastroenterology and Motility (JNM Vol.1 No.-

Role of palliative surgery in incurable stage IV colorectal cancer

Seung-Kook Sohn(손승국) 대한외과학회 2014 대한외과학회 학술대회 초록집 Vol.2014 No.11

Analysis of Symptom Determinant of Cucumber mosaic virus RNA3 via Pseudorecombinant Virus in Zucchini Squash

Choi, Seung-Kook,Yoon, Ju-Yeon,Choi, Jang-Kyung,Kim, Kook-Hyung,Sohn, Seong-Han The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.4

Isolates of Cucumber mosaic virus (CMV) collected in Korea, were compared with their pathological features in tobacco and zucchini squash. Full-length cDNA clone of RNA3 was generated by using long-distance RT-PCR. Transcript RNA3 from the cDNA clone was inoculated onto host plants with transcripts RNA1 and RNA2 of Fny strain, generating RNA3-pseudorecombinant CMV. Timing and severity of systemic symptom was not significantly different among the pseudorecombinant CMVs in tobacco, compared with strains Fny-CMV and Pf-CMV. However, the pseudorecombinant CMVs induced two different systemic symptoms (mosaic vs. chlorotic spot) in zucchini squash. Based on symptom induction, the pseudorecombinant CMVs were categorized into two classes. The severity and timing of symptoms were correlated with viral RNA accumulations in systemic leaves of zucchini squash, suggesting that different kinetics of virus movement associated with CMV proteins are crucial for systemic infection and symptom development in zucchini squash. The analysis of movement proteins (MP) of CMV strains showed high sequence homology, but the differences of several amino acids were found in the C-terminal region between Class-I-CMV and Class-II-CMV. The analysis of coat proteins (CP) showed that the CMV isolates tested belonged to CMV subgroup I and the viruses shared overall 87-99% sequence identity in their genomes. Phylogenetic analysis of MP and CP suggested that biological properties of Korean CMV isolates have relationships associated with host species.

On the Ship Manoeuvring Simulator Newly Developed by Korea Maritime University

Sohn, Kyoung-Ho,Kim, Jin-Kook,Yang, Seung-Yeul 한국항해항만학회 2002 International journal of navigation and port resea Vol.1 No.1

Ship manoeuvring simulator has been widely utilized for training mariners, for assessing safety, for developing harbour and port, and for designing ships. We discuss a ship manoeuvring simulator which has been newly developed by Korea Maritime University. We briefly explain the computer network system connected with each other, the interface system between computers and navigational instruments, the mathematical model of ship manoeuvring motion in harbour areas, and also ship and terrain modeling technique. Finally using the simulator an experiment of distance cognition and a real time simulation of berthing/deberthing manoeuvre carried out and the results are shown.

직장질루(Rectovaginal Fistula) 62예의 임상적 분석

강승현,김남규,임대진,손승국,민진식 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.1

Analysis of the Complete Genome Sequence of Zucchini yellow mosaic virusstrain A Isolated from Hollyhock

Seung Kook Choi,Ju Yeon Yoon,Seong Han Sohn 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.4

Does hyaluronic acid stimulate tumor growth after endoscopic mucosal resection?

Sohn, Dae Kyung,Chang, Hee Jin,Choi, Hyo Seong,Jeong, Seung-Yong,Kook, Myeong-Cherl,Kim, Chan Gyoo,Choi, Il Ju Blackwell Publishing Asia 2008 Journal of gastroenterology and hepatology Vol.23 No.8

<P>Abstract</P><P>Background and Aim: </P><P>A submucosal injection of sodium hyaluronate is widely used for mucosal elevation in endoscopic mucosal resection (EMR) or endoscopic submucosal dissection procedures; however, the oncologic safety of sodium hyaluronate remains unknown. Hyaluronate is the main ligand for CD44 and this interaction was reported to promote tumor progression in <I>in vitro</I> or animal studies. This study aimed to evaluate the effects of sodium hyaluronate on tumor growth after EMR for gastrointestinal cancers.</P><P>Methods: </P><P>The study included 18 consecutive patients who underwent surgery for locally-recurrent or remnant gastrointestinal cancers after EMR from January 2001 to December 2006. The immunohistochemical expression levels of Ki-67, CD44, ErbB2, and epidermal growth factor receptor (EGFR) were evaluated in the primary tumor tissue and the recurrent tumor. The protein expression in recurrent or remnant lesions was also compared between the sodium hyaluronate group and non-sodium hyaluronate group.</P><P>Results: </P><P>Sodium hyaluronate was used in nine of 14 cases with EMR for gastric cancers and in one of four cases for colon cancers. The time to operation after EMR was 133 days (5–687 days). An analysis of the immunohistochemical expression levels between primary and recurrent or remnant tumors showed no significant differences in the expression levels of Ki-67, CD44, ErbB2, and EGFR with or without sodium hyaluronate.</P><P>Conclusions: </P><P>We found no evidence that sodium hyaluronate stimulates the growth of remnant tumors after EMR.</P>