동아(Benicasa hispida)첨가 식이가 비만을 유도한 흰쥐의 혈청성분에 미치는 영향

이승훈,김송전 명지대학교 자연과학연구소 1998 자연과학논문집 Vol.17 No.-

본 실험은 최근 생활수준의 향상과 이에 따른 식생활, 생활방식의 변화로 인해 심각한 사회문제로 대둑되고 있는 비만증과 여러 가지 합병증의 식이를 통한 개선과 치료를 목적으로, 한방 및 민간요법에서 비만방지 효과가 있다고 알려진 국내에서 생산되는 생약재, 특히 동아의 비만증 억제 기능성의 탐색을 하기 위해 행해졌다. Sprague-Dawley계 웅성 흰쥐에게 비만을 유발하기 위한 cafeteria diet를 급여하면서 17종의생약재의 열수추출물을 흰쥐 체중 100g당 0.4ml씩 각각의 군에 하루 2회씩 6주간 경구 투여한 후, 쥐의 체중증가량, 에너지섭취량 및 fat pad의 무게를 측정하였고, 간의 중성지질 함량, 혈당량 및 혈중 콜레스테롤 수치를 근거로 atherogenic index를 분석하였다. 그 중 가장 뛰어난 효과를 보인 동아의 수확시기별 비만억제 효과와 그 성분분석을 하여 다음과 같은 결론을 내렸다. 본 실험에 사용된 17종의 시료들의 배합으로 비만증 및 이와 관련된 여러 성인병의 예방과 치료에 도움이 될 수 있는 기능성 식품의 개발 가능성의 제시와 ,과거엔 상식해 왔으나 현재는 잊혀진 동아의 뛰어난 비만증억제 기능성의 재발견을 토대로 앞으로 동아의 건조,추출, 분획 및 절임 조건이 비만증 억제효과에 미치는 영향과 동아가 비만증을 억제 할 수 있는 것으로 생각된다. Obesity and it's various complication like diabetes melitus coronary heat disease, hepatitis, cholelithiasis, gout, nephritis were been serious social problem in these days. The purpose of this study was to investigate the anti-obesity function of wax gourd. The experiment using Sprague-Dawley male rats showed that seventeen kinds of food materials inhibited the increase of body weight gain, energy intake, fat pad weight, live triglyceride content, atherogenic index, and blood glucose concentration induced by cafeteria diet. Oral administration of wax gourd decreased the most body weight gain, energy intake, fat pad weight, and blood glucose concentration. Liver triglycerides content was the lowest in the group adiministered mulberry stem extract, and the group administered pine leaves showed the lowest atherogenic index. The combined administration of wax gourd, mulberry stern extract and pine leaves improved more the obesity and the related sympotms than the individual administrations. the above results show that the functional food for obesity and the related diseases of adult people can be developed by the conbination of the 17 food materials screened in this study.

Recolonization of benthic macroinvertebrates after anthropogenic disturbance in natural streams, South Korea

Seung-phil Chun,Seung-hoon Chon,Seung-oh Lee,Jang-hyuk Im,Woo-kyun Lee,Myoung-chul Kim 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.2

Stream ecosystems are closely related to many human activities. Therefore, streams are affected by anthropogenic disturbances such as riverine development and gravel-mining as well as deterioration of water quality. The goal of this study was to elucidate the recolonization process of the macroinvertebrate community after a small-scale anthropogenic disturbance. Field studies were conducted at three sites in a natural stream. The number of recolonizing species tended to increase slightly over time, exceeding the total species number of the control. Ephemeroptera contributed the most to shaping the recolonizing pattern of the entire community. From the result of changes in dominant species, the early recolonizers of each site were the species that showed more frequent occurrence particulary at each sites. But the late recolonizers are Chironomidae at all the sites commonly. This result implies that the actual differences exist among the recolonizing trends of each benthic macroinvertebrate taxon. Collector-gatherers and scrapers comprised about 70% of the recolonizing species. These results indicate that the recolonizing process of an aquatic community after an artificial disturbance depends on the environmental conditions(particularly substratum composition or organic pollution) of the habitat.

Recolonization of benthic macroinvertebrates after anthropogenic disturbance in natural streams, South Korea

( Seung Phil Chun ),( Seung Hoon Chon ),( Seung Oh Lee ),( Jang Hyuk Im ),( Woo Kyun Lee ),( Myoung Chul Kim ) 한국환경생태학회 2015 한국환경생태학회지 Vol.29 No.2

Stream ecosystems are closely related to many human activities. Therefore, streams are affected by anthropogenic disturbances such as riverine development and gravel-mining as well as deterioration of water quality. The goal of this study was to elucidate the recolonization process of the macroinvertebrate community after a small-scale anthropogenic disturbance. Field studies were conducted at three sites in a natural stream. The number of recolonizing species tended to increase slightly over time, exceeding the total species number of the control. Ephemeroptera contributed the most to shaping the recolonizing pattern of the entire community. From the result of changes in dominant species, the early recolonizers of each site were the species that showed more frequent occurrence particulary at each sites. But the late recolonizers are Chironomidae at all the sites commonly. This result implies that the actual differences exist among the recolonizing trends of each benthic macroinvertebrate taxon. Collector-gatherers and scrapers comprised about 70% of the recolonizing species. These results indicate that the recolonizing process of an aquatic community after an artificial disturbance depends on the environmental conditions(particularly substratum composition or organic pollution) of the habitat.

Recolonization of benthic macroinvertebrates after anthropogenic disturbance in natural streams, South Korea

Chun, Seung-Phil,Chon, Seung-Hoon,Lee, Seung-Oh,Im, Jang-Hyuk,Lee, Woo-Kyun,Kim, Myoung-Chul Korean Society of Environment and Ecology 2015 한국환경생태학회지 Vol.29 No.2

Stream ecosystems are closely related to many human activities. Therefore, streams are affected by anthropogenic disturbances such as riverine development and gravel-mining as well as deterioration of water quality. The goal of this study was to elucidate the recolonization process of the macroinvertebrate community after a small-scale anthropogenic disturbance. Field studies were conducted at three sites in a natural stream. The number of recolonizing species tended to increase slightly over time, exceeding the total species number of the control. Ephemeroptera contributed the most to shaping the recolonizing pattern of the entire community. From the result of changes in dominant species, the early recolonizers of each site were the species that showed more frequent occurrence particulary at each sites. But the late recolonizers are Chironomidae at all the sites commonly. This result implies that the actual differences exist among the recolonizing trends of each benthic macroinvertebrate taxon. Collector-gatherers and scrapers comprised about 70% of the recolonizing species. These results indicate that the recolonizing process of an aquatic community after an artificial disturbance depends on the environmental conditions(particularly substratum composition or organic pollution) of the habitat.

Non-invasive assessment of changes in Liver fibrosis via Liver stiffness measurement in patients with chronic hepatitis B: Impact of antiviral treatment on fibrosis regression

Seung Up Kim,Jun Yong Park,Do Young Kim,Sang Hoon Ahn,Eun Hee Choi,Jae Yeon Seok,Jung Min Lee,Young Nyun Park,Chae Yoon Chon,Kwang Hyub Han 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.3(S)

HBV : PE-033 ; Association between IL28B polymorphism and spontaneous clearance of hepatitis B virus

( Seung Up Kim ),( Hye Young Chang ),( Jun Yong Park ),( Do Young Kim ),( Kwang Hyub Han ),( Chae Yoon Chon ),( Sang Hoon Ahn ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: The association between IL28B polymorphism and spontaneous clearance of HBV infection has been available in only one study, reported as a brief article. Thus, we analyzed this association in a large cohort with chronic hepatitis B (CHB) and investigated whether there is any novel polymorphism in the DNA sequence of IL28B genome. Methods: Between January 2007 and June 2010, a total of 208 patients who have suffered from CHB infection and newly diagnosed CHB-related hepatocellular carcinoma (HCC) were prospectively recruited and defined as CC group [HBsAg (+) for over 6 months, anti-HBc (+), and anti-HBs (-)]. During the study period, a total of 351 living liver or kidney donors were also prospectively recruited and stratified into UE group [n=106; HBsAg (-), anti-HBc (-), and anti-HBs (-)] or SC group [n=245; HBsAg (-), anti-HBc (+), and anti-HBs (+)] according to their HBV serological markers. For the detection of the three SNPs (rs12979860, rs12980275, and rs8099917) near the IL28B gene, primer extension reactions were performed using SNaPshot ddNTP Primer Extension Kit (Applied Biosystem, Foster City, CA). Nine primer sets were used to amplify IL28B coding region. Results: All patients had HBV genotype C. The non-genetic factors including age and male gender was similar among the groups (all p>0.05). Regardless of groups, rs12979860 CC was most frequently identified in more than 85% of patients in each group (85.0% in UE, 85.9% in SC, and 93.5% in CC group, respectively) whereas rs12979860 TT were not identified in any group (0% in all groups). Similarly, rs12980275 AA and rs8099917 TT was most frequently identified (≥85%), regardless of subject groups, whereas rs12980275 GG was identified in only one subject in SC group and rs8099917 GG was not identified. Using randomly selected 50 samples from each group for full sequencing of IL28B coding region, we found 19 novel SNPs. Of these, 6 SNPs (SNP2, 5, 7, 8, 17, and 19) were significantly different among UE, SC, and CC groups (all p<0.05). Regardless of groups, SNP2 TT (84.0~86.3%), SNP5 CC (92.0~97.1%), SNP7 GG (84.0~92.7%), SNP8 GG (84.0~ 94.7%), SNP17 AA (94.0~98.8%), and SNP19 GG (82.0~ 93.0%) was most frequently identified. Conclusions: Our data suggest that the SNP upstream of IL28B has no effect on HBV recovery. Additional studies are needed to understand the mechanisms underlying the beneficial effect of this SNP in HCV infection but not in HBV infection and investigate the clinical implication of the newly identified 6 SNPs in IL28B genome.

The Relationship between Type 2 Diabetes Mellitus and Non-Alcoholic Fatty Liver Disease Measured by Controlled Attenuation Parameter

Chon, Young Eun,Kim, Kwang Joon,Jung, Kyu Sik,Kim, Seung Up,Park, Jun Yong,Kim, Do Young,Ahn, Sang Hoon,Chon, Chae Yoon,Chung, Jae Bock,Park, Kyeong Hye,Bae, Ji Cheol,Han, Kwang-Hyub Yonsei University College of Medicine 2016 Yonsei medical journal Vol.57 No.4

<P><B>Purpose</B></P><P>The severity of non-alcoholic fatty liver disease (NAFLD) in type 2 diabetes mellitus (T2DM) population compared with that in normal glucose tolerance (NGT) individuals has not yet been quantitatively assessed. We investigated the prevalence and the severity of NAFLD in a T2DM population using controlled attenuation parameter (CAP).</P><P><B>Materials and Methods</B></P><P>Subjects who underwent testing for biomarkers related to T2DM and CAP using Fibroscan® during a regular health check-up were enrolled. CAP values of 250 dB/m and 300 dB/m were selected as the cutoffs for the presence of NAFLD and for moderate to severe NAFLD, respectively. Biomarkers related to T2DM included fasting glucose/insulin, fasting C-peptide, hemoglobin A1c (HbA1c), glycoalbumin, and homeostasis model assessment of insulin resistance of insulin resistance (HOMA-IR).</P><P><B>Results</B></P><P>Among 340 study participants (T2DM, n=66; pre-diabetes, n=202; NGT, n=72), the proportion of subjects with NAFLD increased according to the glucose tolerance status (31.9% in NGT; 47.0% in pre-diabetes; 57.6% in T2DM). The median CAP value was significantly higher in subjects with T2DM (265 dB/m) than in those with pre-diabetes (245 dB/m) or NGT (231 dB/m) (all p<0.05). Logistic regression analysis showed that subjects with moderate to severe NAFLD had a 2.8-fold (odds ratio) higher risk of having T2DM than those without NAFLD (<I>p</I>=0.02; 95% confidence interval, 1.21–6.64), and positive correlations between the CAP value and HOMA-IR (ρ=0.407) or fasting C-peptide (ρ=0.402) were demonstrated.</P><P><B>Conclusion</B></P><P>Subjects with T2DM had a higher prevalence of severe NAFLD than those with NGT. Increased hepatic steatosis was significantly associated with the presence of T2DM, and insulin resistance induced by hepatic fat may be an important mechanistic connection.</P>

LC, Acute : PE-114 ; FibroScan based risk estimation of HBV-related HCC occurrence: development and validation of a predictive model

( Seung Up Kim ),( Do Young Kim ),( Ki Jun Song ),( Jun Yong Park ),( Sang Hoon Ahn ),( Kwang Hyub Han ),( Chae Yoon Chon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Recently, liver stiffness measurement (LSM) using transient elastography (TE; FibroScan(R)) has been introduced as a promising non-invasive device for assessing the degree of liver fibrosis. The aim of this study is to develop and validate a LSM-based predictive model for HCC occurrence in a large cohort of patients with CHB. Methods: The study cohort consisted of 1,110 patients who visited out-patient clinic between May 2005 and December 2007 and was used to develop the predictive model for HCC occurrence. Results: The mean age of our cohort was 50.2 year (755 men). During the median follow-up of 30.7 years, 55 patients developed HCC. On multivariate analysis, age, male gender, and LSM values were selected as independent predictor of HCC occurrence (all p<0.05) whereas HBV DNA ≥20,000 IU/L showed the borderline statistical significance (p=0.0659). In addition to 3 independent variables of age, male gender and LSM value, HBV DNA ≥20,000 IU/L was also incorporated as a constituent variable to develop a predictive model for HCC occurrence, which has previously shown to be an important long-term risk predictor of HCC development. Probability = 1-PA A = exp (0.05306 x age + 1.106 x male gender + 0.04858 x LSM values + 0.50969 x HBV DNA ≥ 20,000 IU/L) This newly developed predictive model showed a fairly good discrimination capability, with AUROC of 0.806 (95% confidence interval, 0.738-0.874). For assessing the discrimination, we used 10-fold cross-validation, in which patients were randomly divided into ten groups and the AUROCs were recalculated after removing 10% of patients of full dataset. The AUROCs remained largely unchanged, with the average AUC of 0.813 (95% confidence interval, 0.804-0.822). We plotted a calibration chart for predicted and observed risk of HCC occurrence (figure 1). The predicted risk of HCC occurrence calibrated fairly well with the observed risk with a correlation coefficient of 0.905 (p<0.001). Conclusions: We developed a LSM-based HCC prediction model using four variables of age, gender, HBV DNA ≥20,000 IU/L, and LSM value, which showed a fairly good discrimination capability in patients with CHB. However, this predictive model needs external validation in patients of other institute and other ethnic origins.

HBV : PE-033 ; Association between IL28B polymorphism and spontaneous clearance of hepatitis B virus

( Seung Up Kim ),( Hye Young Chang ),( Jun Yong Park ),( Do Young Kim ),( Kwang Hyub Han ),( Chae Yoon Chon ),( Sang Hoon Ahn ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: The association between IL28B polymorphism and spontaneous clearance of HBV infection has been available in only one study, reported as a brief article. Thus, we analyzed this association in a large cohort with chronic hepatitis B (CHB) and investigated whether there is any novel polymorphism in the DNA sequence of IL28B genome. Methods: Between January 2007 and June 2010, a total of 208 patients who have suffered from CHB infection and newly diagnosed CHB-related hepatocellular carcinoma (HCC) were prospectively recruited and defined as CC group [HBsAg (+) for over 6 months, anti-HBc (+), and anti-HBs (-)]. During the study period, a total of 351 living liver or kidney donors were also prospectively recruited and stratified into UE group [n=106; HBsAg (-), anti-HBc (-), and anti-HBs (-)] or SC group [n=245; HBsAg (-), anti-HBc (+), and anti-HBs (+)] according to their HBV serological markers. For the detection of the three SNPs (rs12979860, rs12980275, and rs8099917) near the IL28B gene, primer extension reactions were performed using SNaPshot ddNTP Primer Extension Kit (Applied Biosystem, Foster City, CA). Nine primer sets were used to amplify IL28B coding region. Results: All patients had HBV genotype C. The non-genetic factors including age and male gender was similar among the groups (all p>0.05). Regardless of groups, rs12979860 CC was most frequently identified in more than 85% of patients in each group (85.0% in UE, 85.9% in SC, and 93.5% in CC group, respectively) whereas rs12979860 TT were not identified in any group (0% in all groups). Similarly, rs12980275 AA and rs8099917 TT was most frequently identified (≥85%), regardless of subject groups, whereas rs12980275 GG was identified in only one subject in SC group and rs8099917 GG was not identified. Using randomly selected 50 samples from each group for full sequencing of IL28B coding region, we found 19 novel SNPs. Of these, 6 SNPs (SNP2, 5, 7, 8, 17, and 19) were significantly different among UE, SC, and CC groups (all p<0.05). Regardless of groups, SNP2 TT (84.0∼86.3%), SNP5 CC (92.0∼97.1%), SNP7 GG (84.0∼92.7%), SNP8 GG (84.0∼94.7%), SNP17 AA (94.0∼98.8%), and SNP19 GG (82.0∼ 93.0%) was most frequently identified. Conclusions: Our data suggest that the SNP upstream of IL28B has no effect on HBV recovery. Additional studies are needed to understand the mechanisms underlying the beneficial effect of this SNP in HCV infection but not in HBV infection and investigate the clinical implication of the newly identified 6 SNPs in IL28B genome.

Improvement of Liver Fibrosis after Long-Term Antiviral Therapy Assessed by Fibroscan in Chronic Hepatitis B Patients With Advanced Fibrosis :

Chon, Young Eun,Park, Jun Yong,Myoung, Sung-Min,Jung, Kyu Sik,Kim, Beom Kyung,Kim, Seung Up,Kim, Do Young,Ahn, Sang Hoon,Han, Kwang-Hyub American College of Gastroenterology 2017 The American journal of gastroenterology Vol.112 No.6

<P>CONCLUSIONS: In CHB patients with advanced fibrosis receiving antiviral treatment, annual LS measurement revealed that fibrosis improvement slows but continues during treatment. Low LS value (< 12.0 kPa) at baseline was a significant predictor for 5-year fibrosis improvement.</P>