서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

장기표면의 내외봉한관과 봉한소체의 형태학적 관찰

안성훈 ( Seong Hun Ahn ),김민수 ( Min Su Kim ),이상훈 ( Sang Hun Lee ),권오상 ( O Sang Kwon ),김재효 ( Jae Hyo Kim ),소광섭 ( Kwang Sup Soh ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2009 Korean Journal of Acupuncture Vol.26 No.1

Objective: In 1960`s Bonghan Kim`s team found BongHan(BH) ducts which were presumed as acupuncture meridians and BH corpuscles. They asserted Bonghan theory and SanAl theory which was involved in cell division and cell restoration. However, many other experiments which had been operated to demonstrate and find the existence of BH ducts had failed because of the secret of blue stain drugs. During the last several years, BongHan theory has been revived through experimental researches to find the anatomical structures of BH ducts and corpuscles by Soh`s Biomedical Physics Lab. Soh`s research team used the staining with Janus Green B, Alcian blue, nanoparticles and Acridine Orange. We used DAPI staining to find the existence of BH ducts and the corpuscles and to observe nuclear arrangement. Methods: We used japan white rabbits as experimental animals. BH ducts and corpuscles were stained with DAPI. The nucleus configuration in BH ducts stained with DAPI were observed with microscope. Results: In this study, we found thread like structures in silver white color distinguished from the blood vessels, nerves and lymph vessels. These thread like vessels in the linear duct shape were connected to same colored mass in the ball shape. Thread like structures we found could be separated easily from the surrounding other organ mass. The nuclei of the thread like structure in DAPI staining, are about 10~20㎛ length, in rod shape and linear arrangement. Conclusion: We concluded that the thread like structure we found was same vessel reported by Soh`s research team, BongHan ducts and corpuscle.

소아 두뇌외상의 임상적 고찰

서성일,송시헌,김성호,김관태,김윤 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.2

The clinical analysis was carried out with 182 cases of craniocerebral injuries under 15 years of age, admitted at the Department of Neurosurgery, Chung Nam National Universty Hospital during the period from September, 1990 to September 1993. Following results were obtained : 1) There were 133 males and 49 females and sex ratio was 2.7% 2) Age incidence of the accidents was greatest at 6 years of age and mostly distributed to the age group between 4 to 7. 3) Seasonal peak of the accidents was at May. 4) The accident occurred mostly botween 4 P.m. and 6 P.m in a day.5) The most frequent cause of the craniocerebral injuries was traffic accident(105cases, 57.69%) 6) No deterioration of cansciousness before and after the admission was doserved in 68cases(37.36%) 7) Pupillary change was observed in 25 cases and Babinski sign was elicitable in 31cases 8) Early convulsion and vomiting were frequent symptoms in childhood. 9) Skull fracture was observed in 72 cases(39.56%) in which linear fracture was most frequent and predilection site was parietal bone. 10) The accompanying rate of skull fracture in intracranial hemorrhage was 65.54% 11) Associated injures were found in 44 cases(24.18%). The frequent ones were e xtremity fracture, clavicle fracture 12) The overall mortality was 7.14%.

有機色素分子와 陰이온 界面活性劑 사이의 相互作用에 關한 吸收分光學的 硏究

송기동,김일두,박성규,김준태,김성현 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

The metachromatic behavior of methylene blue (MB) ia aqueous solution and in the presense of sodium dodecyl sulfate (SDS) have been studied by the absorption spectroscopic method. The results are as follows. The association force of the MB molecules in aqueous solution seems to be complex of three factors, i.e., London's dispersion force, the hydrophobic interaction and the interaction between permanent dipoles it has been measured of critical micelle concentration (CMC) in aqueous solution of SDS and that of MB-SDS to be 8.0mM and 7.9mM respectively. This indifference between CMC values of the pure SDS and MB-SDS system would mean the formation of dye-rich induced micelles(drim). The spectral changes of MB-SDS system with the addition of the inorganic salts have been observed. The addition of the salt into the solution causes the redistribution of MB molecules in the complex(D_n^+ S_m^-). It would be resulted from the decrease of the stacking effect between the bound MB molecules. The spectral changes observed in the MB-SDS system with the increase of the S/D values are analyzed and the behavior of the meta band is explainsd in terms of the stacking theory and micelle formation theory

원발성 유방암에서 Sodium Iodide Symporter의 발현과 ^(99m)Tc-MIBI 유방스캔의 관계

석주원,김성장,곽희숙,이창훈,김인주,김용기,배영태,김동수 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.6

목적: hNIS는 갑상선 조직 외에 다른 조직들에서도 발현된다고 알려져 있다. 유방암 세포는 그런 조직들 중에 하나이며, 그에 의한 유방암의 방사성옥소치료의 가능성이 제시되고 있다. 본 연구에서는 유방암 조직에서 hNIS의 발현정도를 알아보고, ^99mTc-MIBI 유방스캔과 hNIS의 발현 정도와의 관계를 조사하여 보았다. 대상 및 방법: 본 연구에서는 유방암으로 수술을 시행했던 56명의 환자를 대상으로 하였다. hNIS의 발현은 면역조직화학염색에 의해서 평가되었으며, 그 결과를 ^99mTc-MIBI 유방스캔의 판정 결과와 비교하였다. 결과: 전체 56명의 환자에서 hNIS의 발현율은 41.1%였다. 병리학적 진단에 의해 침윤성관상피암종이었던 49명에서의 발현율은 42.9%, 관상피내암종이었던 7명에서의 발현율은 28.6%였다. ^99mTc-MIBI 유방스캔에서 국소섭취 소견이 관찰되었던 41명에서의 hNIS의 발현율은 31.7%였다. 비정상적인 섭취 소견이 관찰되지 않았던 15명의 환자에서의 hNIS의 발현율이 의미있게 높게 관찰되었다(66.7%, p>0.05). 결론: 유방암 환자에서의 hNIS의 발현율은 그다지 높지 않았다. ^99mTc-MIBI 유방스캔에서 섭취증가 소견이 없을 때 hNIS의 발현율이 더 높았다. Purpose: Human Na^+/I- symporter (hNIS) is known to be expressed in many tissues other than thyroid gland. The breast cancer cells are one of them and the possibility of radioiodine therapy in treatment of the breast cancer may be suggested. We investigated the expression rate of hNIS and the relationship between the expression of hNIS and the finding of 99mTc-MIBI scintimammography in the breast cancer. Materials and Methods: Surgically proved 56 patients with breast cancer were the subjects of this study. The expression of hNIS were evaluated by immunohistochemistry and the results were compared to the findings of 99mTc-MIBI scintimammography. Results: Overall expression rate of hNIS was 41.1% in 56 patients. According to the pathologic diagnosis, it was 42.9% in 49 patients with invasive ductal carcinoma and 28.6% in the 7 patients with ductal carcinoma in situ. The expression rate of hNIS in the 41 cases with a focal increased uptake at he breast lesion on 99mTc-MIBI scintimammogram was 31.7%. That in the 15 cases without any abnormal uptake on the scan was significantly higher(66.7%, p<0.05). Conclusion: The expression rate of hNIS in the patients with breast cancer was not so high. The rate was higher in the patients with no increased uptake at the breast lesion on 99mTc-MIBI scintimammography. (Korean J Nucl Med 2002;36;325-32)

갈치속젓의 휘발성성분에 관한 연구

김훈,장성민,윤성숙,박승영,김소정,차용준 Institute of Genetic Engineering Changwon National 1999 Gene and Protein Vol.3 No.1

Volatile components in salt-fermented hair tail viscera were anlayzed by simultaneous steam distillation-solvent extraction method and gas chromatography/mass spectrometry method. Seventy four compounds were detected and composed mainly of aldehydes(15 compounds), ketones(7), alcohols(13), nitrogen containing compounds(5), esters(6), sulfur containing compounds(4), aromatic compounds(10) and miscellaneous compounds(14). Among these, some compound groups which were known to have generally the low flavor thresholds, such as aldehydes, ketones, nitrogen containing compounds, esters having low molecular weight and sulfur containing compounds, were supposed to be having major effects on the overall flavor of salt-fermented hair tail viscera.

울산만내의 2차원 분산현상에 관한 연구

김성득,고성훈 울산대학교 1998 공학연구논문집 Vol.29 No.2

해양 오염 문제의 효과적인 대처방안은 오염물질의 분산현상을 규명함으로써 오염원에 의한 시간적, 공간적 농도분포를 예측하고 이들 오염원에 의한 피해가 없도록 관리하는 것이다. 울산만은 평균 대조차 48.2㎝의 조석과 태화강 유입수에 의한 해수유동이 발생하고 있어 이에따른 오염물질의 분산현상을 파악하여야 한다. 본 논문에서는 해수유동 현상은 Navier - Stokes 방정식을 유한차분 양해법으로 구한 Christopher G. Koutitas(1988)의 모델을 사용하였고, 여기서 계산된 해수유동 속도를 사용하여 수심방향으로 적분된 2차원 분산방정식을 2차의 삼각형요소를 사용한 유한요소법에 의해 해석하였다. 계산결과를 측정치와 비교 검정을 하지는 않았으나 하구만에서의 분산에 관한 정성적 현상을 파악하는데는 크게 유용하게 활용될 것으로 생각된다. An effective counterplan of sea pollution problem is preestimating the concentration distribution depending on time and place through finding out the dispersion phenomena of pollutants, and managing not to be damaged by such pollutants. There are 48.2 ㎝ of mean spring tide and outflow of Taewha river in Ulsan Bay, so the dispersion phenomena should be calculated according to these conditions. In this paper the flow problem of sea area was calculated using the Christoper G. Koutitas finite difference model in solving Navier-Stokes equation, and using these velocity results the two dimensional dispersion equation which is integrated according to depth was calculated using the finite element method of quadratic triangular elements. The calculated results was not verified comparing to observed data but this results should be very useful in practical dispersion problems qualitatively in the area of estuaries.

Pb(Zr_0.56 Ti_0.44)O₃+ x[wt%]Cr₂O₃세라믹스의 길이진동에 대한 온도안정성

김동오,한성훈,이개명 濟州大學校 情報通信硏究所 1999 情報通信硏究所論文集 Vol.2 No.-

Recently mobile communication fields need piezoelectric ceramic resonators and filters as possible as small. The length-extensional vibration mode of a rectangular piezoelectric ceramic plate has the advantage of the small size. but the mode has not been studied sufficiently because it was not used extensively until now. In this paper. PZT + x[wt%] Cr₂O₃ ceramics with rhombohedral phase were fabricated. And temperature stability for the mode of the ceramic specimen was investigated. Contrary to our expectations. addition of the stabilizer. Cr₂O₃did not promote the temperature stability for the mode in the PZT ceramic specimen with rhombohedral phase.

렙틴 수용체가 형질 도입된 신경세포에서 ER Stress로 유도된 렙틴저항성에 대한 일산화탄소의 억제작용

김성주,김택상,류현열,정헌택 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

연구 배경 및 목적: 렙틴이 음식의 섭취 양과 체중의 증가를 억제하는 중요한 호르몬 역할을 한다는 사실은 잘 알려졌다. 또한 렙틴에 대한 저항성이 비만의 중요한 원인이라는 연구가 많이 보고되고 있다. 그러나 렙틴 저항성에 대한 구체적인 기전은 아직 밝혀져 있지 않았다. 최근 endoplasmic reticulum (ER) 스트레스에 의한 unfolded protein response (UPR) 가 렙틴에 대한 저항성을 유발한 다는 보고가 있었다. 연구 대상 및 방법: 일산화탄소(carbon monoxide, CO)는 heme oxygenase (HO)-1이 헴(heme)을 산화적 분해로 만들어내는 무색, 무미, 무취의 기체로 항산화, 항염증, 항세포분열 및 항세포고사 작용을 나타내며 조직의 손상에 대한 세포보호작용을 한다. 최근 CO는 ER stress sensor 중의 하나인 protein kinase R-like endoplasmic reticulum kinase (PERK) 를 선택적으로 활성화 시키며 인산화된 PERK 는 HO-1의 전사조절인자인 Nrf2를 인산화로 활성화시켜 결국 HO-1의 유전자 발현을 촉진함이 보고 되였다. 또한 CO로 유도된 PERK의 활성화는 eukaryotic translation initiation factor (eIF) 2a를 인산화로 활성화 시키고 인산화된 eIF2a는 전사인자인 activating transcription factor (ATF)-4의 발현을 유도하지만 CO는 ER stress의 다른 두 개 sensors인 inositol requiring enzyme (IRE) 1a와 하위신호전단물질인 X-box binding protein (xbp)-1이나 ATF6의 활성화에는 영향을 미치지 아니한다. 그러나 ER stress 유발인자들인 thasigargin (TG), tunicamycin (TM), homocysteine 등으로 Xbp-1이나 ATF6가 활성화되는 것을 억제한다. 연구 결과: 본 연구에서는 ER stress 에 의해 활성화되는 UPR에 의해 유발되는 렙틴 저항성이 CO에 의하여 차단될 것이라는 가정을 세우고 다음과 같은 실험결과를 얻었다. TG나 TM에 의해 유도된 ER stress반응 즉 UPR은 렙틴의 신호전달로 야기되는 phospho-signal transducer and activation of transcription (STAT) 3로 인산화되는것을 억제시켰다. 그러나 ER stress를 유도시킬 시 CO를 CO releasing molecule (CORM)으로 처리하였을 때 UPR에 의한 렙틴의 신호전달로 말미암는 STAT3 인산화의 차단이 CO의 양과 노출 시간에 비례하여 제거 되었다. 결론: 이러한 실험결과는 CO에 의한 PERK 인산화는 ER stress 유도체에 의한 IRE1a의 하위 신호전달물질인 Xbp-1 이나 ATF6의 활성화를 억제하여 ER stress 반응인 UPR에 의한 렙틴 저항성을 차단함을 시사한다. 결론적으로 이러한 실험결과는 CO유도체를 이용하여 병인적인 렙틴 저항에 의한 여러 질병을 치료할 수 있음을 시사한다. Background: It is well known that leptin is an important circulating signal for the inhibition of food intake and the gain of body weight. Accumulating data suggest that leptin resistance has been considered to be one of the main causes of obesity. However, the detailed mechanism of leptin resistance is not known yet. Recently, endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) is reported to be responsible for the leptin resistance. Materials & methods: Carbon monoxide (CO), a reaction product of the cytoprotective heme oxygenase (HO)-1, is anti-apoptotic in a variety of models of cellular injury. Exogenous CO activated Nrf2 through the phosphorylation of protein kinase R-like endoplasmic reticulum kinase (PERK), resulting in HO-1 expression. CO-induced activation of PERK was followed by the phosphorylation of eukaryotic translation initiation factor 2a and the expression of activating transcription factor 4. However, CO fails to induce X-box binding protein-1 expression and activating transcription factor 6 cleavage. CO prevented X-box binding protein-1 expression and activating transcription factor 6 cleavage induced by ER-stress inducer such as thasigargin (TG), tunicamycin (TM) and homocysteine. Results: In the present study, we hypothesized that exogenous CO might block the leptin resistance which is induced by UPR during ER stress. Thasigargin or tunicamycin was used to induce ER stress. The activation status of leptin signals were measured by western blotting analysis using a phospho-signal transducer and activation of transcription3 (STAT3) antibody. In this study, ER stress markedly inhibited leptin-induced STAT3 phosphorylation. Conculusion: These results suggest that ER stress induces leptin resistance and exogenous CO-induced phosphorylation of PERK branch reversed ER stress-induced leptin resistance. Moreover, CO releasing molecule (CORM) blocks the inhibition of leptin-induced STAT3 phosphorylation. Together, the pathological mechanism of leptin resistance could be ameliorated by the use of exogenous CO..