Group B Streptococci 감염의 임상양상과 Pulsed-Field Gel Electrophoresis를 이용한 유전자형 분석

이승관,이창규,조경진,김상애,김재영,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Pulsed-field gel electrophoresis (PPGE) has been employed as a tool to study correlation between apparent clinical features and genotyping of Group B Streptococci (GBS) infection. The result of serotyping among 45 isolates showed that serotype la, lb, Ⅲ, Ⅳ, Ⅴ, NT (nontypeable) were 15.5%, 11.1%, 35.5%, 2.22%, 20%, 15.5%, respectively. The result of serotyping for infection group of 26 isolates showed that serotype la, lb, Ⅲ, Ⅳ, Ⅴ, NT were 11.5%, 15.4%, 34.6%, 3.8%, 11.5%, 23%, respectively. The result of serotyping for carrier group of 19 isolates showed that serotype la, lb, Ⅲ, Ⅴ, NT were 21%, 5.3%, 36.8%, 31.6%, 5.3%, respectively. Thus, the predominant serotypes of the infection groups were serotype lb and Ⅲ, and those of the carrier groups serotype Ⅲ and Ⅴ. All isolates were characterized by PFGE analysis. Isolates were assigned to three major phylogenetic Divisions (Ⅰ, Ⅱ, Ⅲ) and subsequently to 16 PFGE groups (A[A1, A2, A3], B[B1, B2, B3], C, D[D1. D2, D3, D4, D5, D6], E[E1, E2], F). Seventy five percentages of Division Ⅰ belonged to the infection group. Seventy eight percentages Division Ⅱ of belonged to the carrier group. One hundred percentages of Division Ⅲ belonged to the infection group. The study on the correlation between genotype and serotype among the isolates showed that serotype Ⅲ was predominant in A1 group (5 of 5 isolates), D1 group (4 of 5 isolates), and F group (2 of 2 isolates). In addition, serotype Ⅴ was the sole serotype in C and D5 group. Those results indicated that serotyping and PFGE group analysis were highly correlated.

소퇴부 심재성 2도 화상 치험 1례

이규영, 유준상 상지대학교 한의학연구소 2022 尙志韓醫論文集 Vol.10 No.1

Objectives: The aim of this study is to report the effect of Korean medical treatment on a deep second-degree burn in the shin area Methods: We treated the female patient suffering from burn injury by Korean medical treatment such as acupuncture, Hwangryunhaedoktang extract, wet dressing of Coptidis Rhizoma distillate, Jawoongo dressing from December 9th 2022 to December 24th 2022. The efficacy of treatment was evaluated by wound area and NRS(Numeral Rating Scale) of pain. Results: After Korean medical treatment, reduction of pain and wound area, burn tissue recovery were observed in a short period of time, and there were no other complications such as infection. Conclusions: This case report showed that the Korean medical treatment is effective in the treatment of deep second-degree burn. More clinical cases will be required in the future.

강박장애와 감각추구 성향

이민규,박상학 中央醫學社 1991 中央醫學 Vol.56 No.5

The purpose of this study is to investigate the relationship between obsessive-compulsive symptoms and sensation-seeking tendency. The subjects were administered the Korean version of Leyton Obsessional Inventory and Sensation Seeking Scale in group. In correlation analysis, the symptom subscale score in Leyton Obsessional Inventory was correlated negatively with unconventional sensation seeking tendency. And then, Using the Leyton Obsessional Inventory, two groups were identified: obsessional group and nonobsessional control group. The author used discriminant function analysis to seek the discriminant sensation seeking behavior between the obsession group and nonobsessional control group. The results of the correlation analysis and discriminant function analysis were as follow. 1. Degree of obsessive symptom was negatively correlated with unconventional sensation seeking tendency. 2. In discriminant function analysis, there was no significant difference of general sensation seeking tendency between the obsessional group and nonobsessional control group.. 3. Specific sensation seeking behaviors, significantly discriminate two groups are as follow. 1) gambling for money 2) using four-letter-words 3) preplaned trip 4) respect for lawful authority 5) parachute jumping 6) good night of rest 7) sexual experience before marriage 8) enjoy discussions insulting others 9) uncontrollable emotional experience

정신분열증 환자의 인지적 결함 : 기억 및 기억 기원의 변별 과정을 중심으로 Memory and Discrimination of The Memory Sources

박상학,신재정,이민규 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.5

The authors investigated the relation between the disturbance of thought and perception and information processing. particulary memory and discrimination of memory sources. in the schizophrenic patients by using experimental procedures. The author tested 20 schizophrenic patients and 20 normal control subjects who were matched according to age. sex and education. All subjects were given 3 types of material set. The first. experimenter presentation set. were made up of 10 opposite word pairs. The second & third set were 10 cards each. A usual word with a cue letter of an opposite word. They were instructed to imagine only with the second set. and to write the opposite word along the cue letter with the third set. After the response sheets had been collected. they were asked to recall all responses in any order. Next. a new test sheet was given for the recognition & the discrimination of the memory sources. The result of the research were as follow: 1) Free recall; A significant decrease of recall ability was found in the schizophrenic group(3.15 words) as compared with the control group(6.68). 2) Recognition(hit rate); There was no significant difference of recognition ability between the SPR group(23.15) and the control group(23.6). 3) Schizophrenic group had poorer signal sensitivity(d'). 4) SPR group had more difficulty in discriminatng what they had imagined from what they had written. SPR group and control group were not different in discriminating what they had seen. 5) Error bias of the discrimination; Schizophrenic group showed markedly poor performance on the discrimination task. They mistook what they had seen as what they had written. what they had imagined as what they had written, and what they had written as what they had seen.

有限要素法에 의한 垂直荷重下의 타이어 變形 및 應力解析

하성호,이규승,박원엽 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.2 No.2

Tires are made of rubber composites which consist of several plies. Each ply consists of a thin layer of rubber matrix reinforced with unidirectional steel or polyester cords. The deformation and stress analysis of reinforced tires is very difficult because of the material properties and complexity of tire composites. In the present work, the tire inflation and contact problem has been solved by the finite element method in which overcome the complexity of tire composites. The finite element formulation is derived from the equilibrium equations by the principle of virtual work, and the constitutive equation given by the rubber material characteristics. Also, a contact formulation is added to the finite element formulation to calculate the stress distribution of tires in contact with flat rigid road under the vertical load. The calculation for the deformed shape and the stress distribution is executed by dividing into many time steps for incremental analysis. And the solutions of each time step are converged by modified Newton-Raphson iteration method. The results of the finite element analysis of the tire deformation and stress distribution, show good agreement with previous similar analysis of others.

남자 주정중독 환자의 아동기 부모-자녀 결합형태와 성격특성과의 관계

김승찬,장경준,이민규 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

저자들은 주정중독 환자의 아동기 부모-자녀 결합형태와 성격 특성을 알아보기 위하여 국립서울정신병원에 입원중인 환자 60명과 같은 병원의 직원을 대상으로 한국판 부모-자녀 결합형태검사(PBI)와 Cattell의 성격요인검사(16PF) 및 아동기 가정환경의 인구학적 지표를 자가보고형 설문지 작성을 통하여 조사하였으며 주요 결과는 다음과 같다. 1) 16PF의 일차요인중 주정중독 환자군에서 지능, 자아강도, 대담성에서 대조군보다 통계적으로 유의하게 낮았으며, 사변성, 죄책감, 자기충족성, 불안감이 통계적으로 유의하게 높았다. 이차요인에서는 주정중독 환자군에서 불안성이 통계적으로 유의하게 높았으며, 자립성과 외향성에서 통계적으로 유의하게 낮았다. 2) PBI의 각 척도에서는 대조군보다 주정중독 환자에서 부친의 과보호가 통계적으로 유의한 정도로 높게 나타났다. 그리고 부친의 과보호는 주정중독 환자의 성격특성으로 나타난 16PF의 각 척도 중 자아강도, 외향성과 부적 상관관계를 보였고 자기충족성, 불안감, 불안성과 정적 상관관계를 보였다. 3) 환자군중 가족력이 없는 환자군과 가족력이 있는 환자군에서, 16PF의 일차요인중 불안감과 이차요인중 불안성에서 가족력이 있는 경우 통계적으로 유의한 정도로 높았다. PBI의 각 척도에서는 가족력 유무에 따른 유의한 차이가 없었다. 본 연구에서는 주정중독 환자는 부친의 과보호와 관련이 있고, 부친의 과보호는 주정중독 환자의 성격특성중 약한 자아강도, 내향성, 자기충독성, 불안감, 불안성과 관련이 있는 것으로 나타났다. 향후 연구방법상의 문제를 보완한 보다 통제된 후속 연구가 기대된다. Alcoholism is the final result of a complex interaction between biological vulnerability and environmental factors such as childhood experience, parental attitudes, social policies, and culture. However, there has been relatively little research in relationship between these environmental factors and personality factors of alcoholism. In order to investigate the relations among parent-child bonding patterns, family environmental factors in childhood and personality factors in alcoholics, the authors administered 16 Personality Factor Questionnaire, Parental Bonding Instrument, and questionnaire for demographic data to 60 alcoholics and 60 healthy person. The results were as follows : 1) Alcoholics showed lower scores in intelligence(B), ego strength(C), boldness(H), independence(IND), extraversion(EXT), and showed higher scores in autia(M), guilt-proneness(O), selfsufficiency(Q₂), anxiety(Q₄), anxiety(ANX) than healthy person. 2) Alcoholics showed higher scores in paternal overprotection. And paternal overprotection was negatively correlated with ego strength(C), extraversion(EXT), and is positively correlated with self-sufficiency(Q₂), anxiety(Q₄), anxiety(ANX). 3) There were no differences in parental representation between alcoholics with family loading and alcoholism without family loading. These results revealed that early parent-child relationship, especially paternal overprotection nright influence some aspects of personality factors in alcoholism. Further studies with well controlled design are needed to confirm our results.

무한요소를 이용한 반무한 구조체의 해석

박광규,이승원,김문겸,황학주 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.2

Half space problems of structural system, which are usually solved using simple truncation of far field or using analytical far field solution, are considered using infinite elements. Among the infinite elements previously developed, a mapped infinite element is adopted and implemented in a finite element code which can handle two dimensional problems. Implemented program is applied to a cavity problem subject to internal pressure. The resulting solutions are compared with boundary element solutions due to Brebbia. In addition, conventional finite element analyses with simple truncation are carried out to compare the efficiency and accuracy of the developed program. The results show that the developed program renders accurate solutions which are close to the known values. Also, it can be seen that the developed program requires a smaller number of elements, so as shorter computing time than the conventional finite element codes, to get the same degree of accuracy.

시험관내에서 카드뮴에 의한 랏트의 신조직 ATPase활성도 억제 양상

김민식,이상준,정규철 중앙대학교 의과대학 의과학연구소 1990 中央醫大誌 Vol.15 No.4

For studying of inhibitory characteristics of cadmium on adenosine triphosphatase (ATPase) in rat kidney, ATPase activities in the microsomal fraction were measured in the presence of varying concentrations of ATP(0.5 to 3.0,mM) and/or cadmium (0.5 to 1,000 μM) in 10mM MgC_2, 100 mM NaCl, 20mM KCI and 125 mM Tris-HCI pH 7.4, or in solutions where NaCl and KCI were replaced with 10 mM ouabain. 1. K_M and Vmax for total ATPase were 0.33 mM and 27.2 μmol/mg protein/hr, respectively, and 0.26 mM and 19.4 μmol/mg protein/hr for Mg^++-ATPase and 0.78 mM and 8.5 μmol/mg protein/hr for Na^+, K^+-ATPase, 2. Inhibition mode of cadmium on total ATPase and Mg^(++)-ATPase was uncompetitive, whereas it was noncompetitive on Na^+, K^+-ATPase. 3. In the presence of 3 mM ATP, the concentrations of cadmium inhibiting the activities of total ATPase, Mg^(++)-ATPase and Na^+, K^+-ATPase to levels of 50% of their original activities (I_(50)) were 176.6 μM, 902.2 μM and 9.2 μM, respectively. Consequently, the activity of Na^+, K^+-ATP ase was inhibited by cadmium as much as 98 times than that of Mg^+-ATPase.

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.

급성 카드뮴중독시 신조직의 Hydrogen peroxide의 생성, Catalase 및 Na^+, K^+ - ATPase활성도의 변화와 Ascorbate, Diethyldithiocarbamate(DDTC), Glutathione 및 α-Tocopherol의 방어효과

김정용,이상준,정규철 중앙대학교 의과대학 의과학연구소 1990 中央醫大誌 Vol.15 No.2

The effects of cadmium and protective effects of ascorbate, diethyldithiocarbarnate (DDTC), glutathione and α-tocopherol on hydrogen peroxide production, catalase and Na^+, K^+-ATPase activities in kidney of rats acutely intoxicated by cadmium were investigated. A single dose of 5mg/kg CdCI_2 were given to all Sprague-Dawley male rats by intraperitoneal injection. In addition, 1 mM/kg ascorbate in Cd + ascorbate-treated group, 500 mg/kg DDTC in Cd + DDTC-treated group, 1 mM/kg glutathione in Cd + glutathione-treated group and 0.5 mM/kg α-tocopherol in Cd + α-tocopherol-treated group were given intraperitoneally 60 min after the cadmium treatment. The saline-treated group was treated with saline only. Rats of each group were sacrificed by decapitation 48 hours after the cadmium treatment. The cadmium concentration in kidney increased with that in blood both in saline and experimental groups. Average cadmium concentrations in blood and kidney of Cd-treated rats were much higher than those in saline-treated group, but no differences were found in average cadmium concentrations in blood and kidney among Cd-treated, Cd + ascorbate-treated, Cd + DDTC-treated, Cd + glutathione -treated and Cd + α-tocopherol-treated groups. In Cd-treated group, hydrogen peroxide production and catalase activity of kidney homogenates was increased but Na^+, K^+-ATPase activity was decreased. Glutathione inhibited cadmium-induced hydrogen peroxide production most effectively. Ascorbate, DDTC and α-tocopherol also inhibited cadmium-induced hydrogen peroxide production. Catalase activity was inhibited in ascorbate-and glutathione-treated groups more than that in Cd-treated group, and no difference was found in catalase activities among Cd + DDTC-treated and Cd + α-tocopherol -treated groups. Inhibition of Na^+, K^+-ATPase activity induced by cadmium was diminished by simultaneous administration of ascorbate, DDTC, glutathione or α-tocopherol. In normal and experimental groups, hydrogen peroxide production increased with increasing cadmium concentrations and the Na^+, K^+-ATPase activity decreased with increasing hydrogen peroxide productions. These results suggest that inhibition of Na^+, K^+-ATPase activity caused by hydrogen peroxide in cadmium intoxicated rats may play a part in cadmium induced renal functional injury. Ascorbate, DDTC, glutathione and a-tocopherol exhibit protective effects against cadmium mediated inhibition of Na^+, K^+-ATPase activity and these effects brought by inhibition of cadmium-induced hydrogen peroxide production.