경구 Ibupenta와 2.5% Indomethacin, 0.25%Desoxymethasone 연고가 UVB로 인한 홍반에 미치는 효과에 대한 연구

조문균 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

The large amount of UVB irradiation of human skin cause acute erythema that is induced by prostaglandin. Ibupenta and indomethacin have antiinflammatory effect by inhibiting prostaglandin pathway. The purpose of this study was to investigate the effect of oral ibupenta and topical 2.5% indomethacin, 0.25% desoxymethasone ointment on erythema induced by UVB irradiation. In this study, healthy sixteen male medical students who did not have any experience of tanning on their back during the last year were included in this study. There 32 volunteers were devided into two groups, oral ibupenta was administrated to one group and not to the other. The minimal erythema dose(MED) of each volunteer was measured with colorimeter. According to the individual's MED, 1 MED, 2 MED,and 3 MED of UVB was irradiated to the volunteer's back, the erythemas before and after the applying the topical agents were evaluated with naked eyes and colorimeter. In group I applied by 2.5% indomethacin, 0.25% desoxymethasone without oral ibupenta, erythemas tested with 2.5% indomethacin were statistically significantly supressed according to data measured by colorimeter. In group Ⅱ applied by 2.5% indomethacin, 0.25% desoxymethasone with oral Ibupenta, erythemas tested with 2.5% indomethacin and 0.25% desoxymethasone were statistically significantly supressed according to data measured by colorimeter. The maximal supression of UVB induced erythema was observed in 2.5% indomethacin with oral ibupenta. 'These data indicate that oral ibupenta, as an inhibitor of prostaglandin, is more effective than topical indometahcin or topical desoxymethasone in inhibition of UVB induced erythema. There is a synergistic effect between oral ibupenta and topical desoxymethasone. This result indicate that 2.5% indomethacin with oral ibupenta is useful therapy in acute UVB induced erythema within 24 hours. To evaluate the erythema, colorimeter is supposed to be more sensitive than naked eye score.

악성흑색종에서 Melanin 세포 이동에 미치는 Endothelin-1 효과에 관한 연구

조문균 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Endothelin-1 is a 21 amino acid vasoconstrictor peptide originally isolated from endothelial cells and keratinocytes. There are three isotype ET-1, ET-2 and ET-3. Only ET-1 involve human skin. Yada group indicated that human melanocyte possesed specific high affinity receptors for ET-1 and that ET-1 stimulated melanocyte proliferation and migratin. The purpose of this study was to determine weather endothelin-1 could enhance melanocyte migration or cell proliferation in malignatn melanoma. We performed Boyden Chamber Checkboard analysis by using control, 20nM, 40nM and 80nM ET-1 concentration in SK 28 cell line and SK30 cell line. The data were represented as percent of cell migration and statistical student's t-test was done. The following results were obtained from this study ; 1. In SK 28 cell line, the percentage of cell migration noted 100.04±10.12% at control, 248.86±6.91% at 20nM, 265.24±9.73% at 40nM and 285.15±8.34 at 80nM. Et-1 significantly (p<0.01) enhanced cell movement at the concentration of 20, 40, and 80 nM. 2. In SK 30 cell line, the percentage of cell migration noted 100.04±10.12% at control, 282.86±8.26% at 20nM, 290.86±7.05% at 40nM and 263.86±4.27 at 80nM. ET-1 significantly (p<0.01) enhanced cell movement at the concentration of 20,40 and 80 nM.

장용학 소설 연구 : 人間救援, 그 永續性의 變奏

정문권,이영조 배재대학교 인문과학연구소 2005 人文論叢 Vol.22 No.-

This dissertation analyzes Jang Yong-hak's fiction 'Archetype's Legend'. He was a writer that stood out in the middle of the time when our nation was suffering deeply. He wanted to present the wicked and wretched wars, which caused mental damages, as a literary work. The book's expression of the negative perspective shows the true wanting of liberty but the impossibility because of the present situation. Jang Yong-Hak used allegory literature technique and imagination to express our nation's sadness in a piece of literatury work. The dissertation is divided as the writer's world of awareness and the main character's awareness. As you trace twisted life of "Lee Jang", you have a glimmer of Author's rebellious spirit about the morbidly contradicting and irrational world. The novel's main thesis is focused on human salvation and to find out the mysterious consequence of people's ethics that opposite the choice of corruption. It demands humans to reflect on one's conduct and about their desirous life.

LETTER TO THE EDITOR : Tripartite Motif-Containing 29 Expression in Squamous Cell Carcinoma

Moon Kyun Cho 대한피부과학회 2012 Annals of Dermatology Vol.24 No.4

A case of herpes zoster that has occurred without chicken pox history in normal early childhood

Cho, Moon Kyun,Lee, Hyun Hee 關東大學校醫科大學醫科學硏究所 1998 關東醫大學術誌 Vol.2 No.1

대상 포신은 주로 성인에서 발생되며, 소아에서는 드물게 발생하는 질환이다. 대상포진의 병변은 군집성 발진이 특징적으로 밴드형태의 분포를 보이며 하나 혹은 그이상의 신경절을 침범하는 질환이다. 원인균은 허피스 바이러스군에 속하는 varicella-zoster바이러스(VZV)이다. 발생기전은 수두를 하는 동안 varicella-virus가 피하신경에 들어가 있다가 varicella-virus가 재활성화 되어 발생된다고 하나 그 이유 아직까지 명확하게 알려지지 않지 있다. 재활성화 시키는 요소는 피로, 연령, 면역 억제제, 림프종, 백혈병 등이 관계된다. 소아기에서 대상포진을 면역상태가 좋지 않은 소아, 자궁내 감염이 있었던 경우, 생후 수두를 앓은 경우 발생한다. 정상 소아의 경우 유아기에 후천적으로 varicella-zoster-virus에 감염되면 대상포진의 발생시기가 단축되며, 된 환아의 경우도 후천적으로 수두백신을 생후 13개월에 접종하였고 그후 13 개월 후 발생된 것으로 보인다. 저자들은 수두의 병력 없이 백신접종 후 발생된 것으로 보이는 대상포진을 경험하였기에 보고하는 바이다.

Elevated KAI1 Protein Expression Identified in Malignant Melanoma

( Moon Kyun Cho ) 대한피부과학회 2013 Annals of Dermatology Vol.25 No.4

Decreased Expression of Type 5 17β-Hydroxysteroid Dehydrogenase (AKR1C3) Protein Identified in Human Diabetic Skin Tissue

( Moon Kyun Cho ) 대한피부과학회 2013 Annals of Dermatology Vol.25 No.4

Background: Diabetes is characterized by chronic hyperglycemia, and hyperglycemia can increase reactive oxygen species (ROS) production from the mitochondrial electron transport chain. The formation of ROS in cells induces oxidative stress and activates oxidative damage-inducing genes. There is no research on the protein levels of oxidative damage-related genes AKR1C3 in human diabetic skin. We explored the expression of AKR1C3 in diabetic skin compared with normal skin tissue. Objective: To compare the expression of AKR1C3 in normal skin versus diabetic skin. Methods: AKR1C3 expression was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was carried out to analyze AKR1C3 expression in the 6 diabetic skin tissue samples (July 2009 to December 2011; Department of Plastic and Reconstructive Surgery at Soonchunhyang University Seoul Hospital, Seoul, Korea). Results: The western blotting showed a significant reduction in AKR1C3 protein expression in diabetic skin tissue compared to normal tissue. Immunohistochemical examination of AKR1C3 showed that it was weakly expressed in all diabetic skin samples. Conclusion: We believe that AKR1C3 is related to diabetic skin in altered metabolic states which elevate ROS production. (Ann Dermatol 25(4) 423∼427, 2013)

Overexpression of KAI1 Protein in Diabetic Skin Tissues

Cho, Moon Kyun,Kwon, Sun Bum,Kim, Chul Han,Lee, Yoon-Jin,Nam, Hae-Seon,Lee, Sang-Han Korean Society of Plastic and Reconstructive Surge 2014 Archives of Plastic Surgery Vol.41 No.3

Background Patients with diabetes mellitus often have a difficult life, suffering from foot ulceration or amputation. Diabetes is characterized by chronic inflammation, and one of the features of inflammation is hypoxia. Recently, it has been reported that KAI1 is a hypoxia target gene. There is no published research on hypoxia-related KAI1 protein levels in human diabetic skin. Therefore, we have investigated the expression of KAI1 protein in diabetic skin tissue in vivo. Methods The expression of KAI1 protein was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was carried out to identify KAI1 expression. Results The western blotting revealed significantly increased expression of the KAI1 protein in diabetic skin tissues as compared to normal skin tissues. Immunohistochemical examination demonstrated that KAI1 was expressed in all diabetic skin tissues with moderate-to-strong positivity and weakly expressed in normal skin tissues. Conclusions Our data suggest that a high expression of the KAI1 protein can be observed in diabetic skin tissue. To the best of our knowledge, this is the first report suggesting that KAI1 protein expression in diabetic skin tissues may be associated with chronic inflammatory states and hypoxia.

Pre-clinical Screening Methods for Evaluating Anti-wrinkle Effect

( Moon Kyun Cho ) 대한화장품학회 2003 대한화장품학회지 Vol.29 No.2

새로운 주름개선 효능물질을 개발하기 위하여 여러 가지 세포배양 모델을 이용한 in vitro 효능물질의 검색방법을 이용하여 후보물질을 도출하고 있다. 이처럼 In vitro에서 선별된 주름개선 후보물질은 사람피부에 직접 도포 하였을 때 in vitro에서 와 는 달리 주름개선 효능이 없는 경우가 많이 있다. 즉 in vitro 검색결과와 사람피부에서의 효능은 현실적으로 큰 차이를 보인다는 것이다. 그러나 모든 효능 후보물질을 사람 피부에 직접 검사할 수는 없는 현실적 어려움이 있다. 이러한 애로점을 해결하기 위하여, 동물모델이나, 인공피부배양모델 등을 이용하는 추세이다. 이번 강의에서, 본인은 동물모델과 in vitro에서 효능 측정방법을 상세하게 리뷰 하고 각 방법에 대해 토의할 것이며, 임상 전 시험 결과를 보고 할 것이다. 그 중 특히 동물 모델은 주름개선 효능물질의 효능을 평가하는데 좋은 대상이 될 것이다. Nowadays, we find out new anti-wrinkle-care-ingredients by in vitro searching methods using many kind of cell-culture-models for investigation of the effective anti-wrinkle-care-ingredients. But, theses new ingredients don't have effect on the human-model for anti-wrinkle, not likely on in vitro. In other words, there are so many differences between the effects on in vitro models and the clinical human models, practically. But, we actually have difficulty in putting all of the new anti-wrinkle-care-ingredients to the test on human models directly. To solve this problem, we have investigated that by using the artificial skin-culture-model or the animal model, In this lecture I will review the detail of assessment method far evaluation of anti-wrinkle agents in vitro and animal model and discuss the pros and cons of each method. Then I will present the results of Preclinical Screening trials, And especially animal model may be a good candidate for evaluation of anti-wrinkle agents.

Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

Cho, Moon Kyun,An, Je Min,Kim, Chul Han,Kang, Sang Gue Korean Society of Plastic and Reconstructive Surge 2014 Archives of Plastic Surgery Vol.41 No.1

Background Aurora kinase A (Aurora-A) plays an important role in the regulation of mitosis and cytokinesis. Dysregulated Aurora-A leads to mitotic faults and results in pathological conditions. No studies on Aurora-A expression in human diabetic skin tissue have been reported. In light of this, we explored the expression of Aurora-A in human diabetic skin tissue. Methods Aurora-A protein was evaluated by western blotting in 6 human diabetic skin tissue and 6 normal skin specimens. Results Increased expression of Aurora-A protein was detected in all diabetic skin tissue samples in both western blot analysis and immunohistochemical staining. However, in the case of the normal skin tissue, no bands of Aurora-A protein were detected in either the western blotting analysis or the immunohistochemical staining. Conclusions Thus far, there have been no studies on the expression of Aurora-A in diabetic skin tissue. However, we believe that oxidative DNA damage related to the expression of Aurora-A protein and Aurora-A could be involved inhuman diabetic skin tissue.