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GlycinecinA의 분비에 관여하는 secretor gene의 cloning
김영미,허정식,허규희 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-
Xanthomonas campestris pv. glycines 8ra produces bacteriocin, glycinecin, which inhibits the growth of bacteria specifically belong to Xanthomonas spp. Xanthomonas spp. includes many important plant pathogenic bacteria such as X. c. pv. vesicatoria which cause the bacterial leaf spot in pepper and tomato and X. oryzae pv. oryzae which causes bacterial leaf blight in rice. Glycinecin has many of good properites as a biological control agent. Since glycinecin inhibit the growth of phytopathogenic Xanthomonas sp, it can be used to control disease without any harm to beneficial bacteria for pant mostly belonged to Pseudomonas sp. However, it needs some modification to develop glycinecin as a effective bilogical control agent. The clone involved in the secretion of bacteriocin production were isolated and named glyAsec.
HL-60 백혈병 세포의 성장에 대한 노랑하늘타리의 효과
김상철,박수영,허규희,김세재,강희경 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-
The purpose of the present study is to examine the inhibitory effect of extracts of Trichosanthes kirilpwii var. japonica on the growth of HL-60 cells and to develop an anti-cancer agent using components of its leaves. To examine the inhibitory effect on the growth, metabolic activity was measured with 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. In results, methanol(MeOH) extract of its leaves and ethylacetate (EtOAc) fraction of MeOH extract markedly inhibited the growth of these cells. At the same time, DNA fragmentation was observed in its extract-treated HL-60 cells. Therefore, inhibitory effect of Trichosanthes kirilpwii var. japonica on the growth of HL-60 seems to arise from the induction of apoptosis.
An angled nano-tunnel fabricated on poly(methyl methacrylate) by a focused ion beam
Her, Eun Kyu,Chung, Hee-Suk,Moon, Myoung-Woon,Oh, Kyu Hwan IOP Pub 2009 Nanotechnology Vol.20 No.28
<P>Angled nano-scale tunnels with high aspect ratio were fabricated on poly(methyl methacrylate) (PMMA) using a focused ion beam (FIB). The fabrication parameters such as ion fluence, incidence angle, and acceleration voltage of the Ga<SUP>+</SUP> ion beam were first studied on the PMMA surface to explore the formation of the nano-scale configurations such as nano-holes and cones with diameter in the range of 50–150 nm at an ion beam acceleration voltage of 5–20 kV. It was also found that the PMMA surface exposed to FIB was changed into an amorphous graphitic structure. Angled nano-scale tunnels were fabricated with high aspect ratio of 700–1500 nm in depth and 60 nm in mean diameter at an ion beam acceleration voltage of 5 kV and under a specific ion beam current. The angle of the nano-tunnels was found to follow the incident angle of the ion beam tilted from 0° to 85°, which has the potential for creating a mold for anisotropic adhesives by mimicking the hairs on a gecko’s feet.</P>
( Kyu Sik Cho ),( Young-Kyu Kim ),( Kyu Hee Her ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Jeju Island is the biggest one in Korea located about 50 miles south of the mainland. The eating habits of Jeju Island natives (JNs) are quite different from those of the mainland people due to the geographic isolation. Diet is one of the main factors affecting the gallstone disease (GD). We investigated the prevalence of GD in both JNs and Jeju Island migrants (JMs) and studied risk factors affecting GD among Jeju Island people. Methods: This study enrolled a total of 20,763 subjects who had medical checkups at the Health Promotion Center of Jeju National University Hospital in Korea from January 2003 to December 2015. They were examined by the ultrasonography if they had gallbladder stones or not. The body Mass index (BMI), biochemical parameters including liver function tests, lipid profile, and fasting blood glucose were checked, and age, birthplace (JNs vs. JMs), and sex were collected based on their medical records. Univariate and multivariate analyses were performed to identify risk factors affecting GD. Results: The prevalence of GD in Jeju Island people was 4.0%; those of JNs and JMs were 3.8% and 4.4% respectively (P = 0.047). In the univariate analysis, risk factors affecting GD were the age, JMs, BMI, fasting blood glucose, high density lipoprotein (HDL)-cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (γ-GTP). In the multivariate logistic regression analysis, independent risk factors were older age, JMs, higher fasting blood glucose and ALT, and lower HDL-cholesterol. Conclusions: The prevalence of GD was significantly lower in JNs than in JMs in Jeju Island. Older age, JMs, higher fasting blood glucose and ALT, and lower HDL-cholesterol may affect the prevalence of GD in Jeju Island.