급성 일산화탄소 폭로가 백서 뇌조직의 immediate early gene, c-fos의 발현에 미치는 영향

하미나,김헌,조수헌,권호장 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.3

The c-fos, an immediate early gene homologous to viral oncogene v-fos, controls protein expression of cells as a DNA transcription factor. Carbon monoxide(CO) may induce tissue hypoxia and neurotoxicity and play an important role in cell signal transduction as a retrograde messenger. However, little is known about the effects of CO on expressions of immediate early genes. This study was performed to evaluate the effects of CO on c-fos expression, and to compare the effects to those of hypoxia in rat brain. Ninety male Sprague-Dawley rats were divided into 3 groups (CO, hypoxic, and ambient air groups), and then exposed to 4,000 ppm CO, 10% O₂-90% N₂gas, or ambient air for 30 minutes, respectively. Immediately, 15 minutes, 30 minutes, 45 minutes, 60 minutes, and 120 minutes after exposure, rat brains were excised and cerebral cortexes, corpus striata, hippocampuses, and cerebellums were separated. Northern blots for c-fos were performed, and GAPDH expression levels were checked as an internal control of total mRNA expression. The c-fos band densities increased till 30 or 45 minutes after exposure. and thereafter decreased. The expression levels of c-fos did not increase in hippocampuses and carebellums, but in cerebral cortexes and corpus striata of hypoxic and CO-exposed groups. In corpus striata of the CO-exposed group, further increase of c-fos expression levels was observed in comparison with the hypoxic group. These findings suggest that the effects of hypoxia and CO are different by the anatomical sites in brain, and CO is involved in signal transduction in corpus striatum, but not in cerebral cortex, hippocampus, and cerebellum within 2 hours after exposure to CO.

철근 또는 강판을 이용한 철근콘크리트 전단벽의 휨성능개선에 관한 실험연구

하기주,서수연,신종학,최민권 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this paper is to study retrofit methods for improving the flexural capacity of RC shear wall. Four RC shear wall specimens are designed and strengthened by using reinforcement or steel plate. Cyclic loads are applied to the specimen under constant axial force. Test result shows that the strength and ductility of specimen is improved when it is strengthened using reinforcement or steel plate. The specimen retrofitted by using steel plate shows high ductility. However the strength contribution of one is not much. It is shown that the most effective method is both to install the additional flexural reinforcement and to confine the ends of wall.

VDT 업무가 근골격계장애에 미치는 영향

권호장,하미나,김돈규,백남종,조수헌,한태륜 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

As use of the visual display terminal(VDT) is becoming more generalized as a result of office automation, the so-called 'VDT syndrome' which is often observed with the workers using VDT has emerged as a serious occupational health problem. However, few comparative study with control group using not only subjective symptom but also physical examination has been conducted. We have conducted a study comparing prevalence rated of musculoskeletal disorder between 113 VDT operators of a telecommunication company and the control groups of housewives and office ladies through a review of subjective symptoms as well as physical examination by physiatrist to understand the magnitude of problem and relative risk of VDT worker. The symptom rates of upper extremity disability in VDT operators were higher than those of controls especially in neck and shoulder area. Fifty-four(47.8%) of 113 VDT operators met our case definition of upper extremity disorder by subjective symptom and physical examination. Myofascial pain syndrome of neck and shoulder muscles were the most common(46.9%). The odd ratio of using VDT operation for developing myofascial pain syndrome in the neck or shoulder area were 2.52(compared to controls of office ladies) and 2.64(compared to controls of housewives). Other anatomical lesion such as elbow, hand and wrist shows slight higher prevalence rates of musculoskeletal disorder than control groups but not statistically significant.

우리 나라 보건관리대행서비스 평가 연구

하은희,조수헌,김선민,주영수,한상환,하미나,권호장,홍윤철,김창엽 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

Group occupational health service programme started in 1990 is one of the measures to cope with limited human and financial resources in occupational health. The programme has expanded rapidly to include 52 institutions, private as well as public, all over the country. In spite of its potential impact on health of employees and practice of occupational health in small and medium sized industries, comprehensive evaluation in terms of quality has not been tried. This study has aims to develop the criteria to assess the quality of newly developed group occupational health service programme, and to investigate the quality of institutions, and finally to develop policies for the quality improvement. 1) Criteria development : By defining occupational health services, in particular for small and medium sized industries, as one of the primary health care, followings are included as core elements of qualitative occupational health programme; accessibility, continuity, comprehensiveness, technical quality, intersectoral collaboration, emphasis on preventive services, community participation, and adequacy. Again each element is divided into five major components of national health system infrastructure developed by the World Health Organization; development of health resources, organized arrangement of resources, delivery of health care, economic support, and management. In turn, each component is categorized into three aspects of quality assessment, structure, process and outcome. Expert panel selected several criteria for each category to evaluate the programme. Criteria were modified according to each group of interviewees, to produce two sets of questionnaire, one for chief operating officer and another for nurses in the institutions, and the chief operating officer and workers in the workplace. 2) Subject : Of all 52 institutions, 25 voluntarily participated in the survey. At individual institution, chief operating officer and practicing nurses were interviewed in depth. After intensive education for interviewees, every interviewees, every interview was performed with standardized guideline and questionnaire. The quality of the 'Group occupational health service programme' was found to be lower than expected. Especially in continuity, comprehensiveness, technical quality, community participation and adequacy, lower quality in structural aspect was commonly identified throughout all the institutions,. Quality in terms of accessibility and continuity highly varied among institutions., To improve quality of the programme, more comprehensive and systematic programme such as accreditation has to be introduced. In addition, human resources, governmental fund and information systems for individual workers are to be developed. As a long range plan, integration of occupational health services into the national health systems and pooling of financial resources and planned allocation should be considered.

가스크로마토그래피를 이용한 요중 마뇨산 측정시 새로운 시료 전처리 방법의 검증

조수현,임용현,김선민,권호장,하미나,한상환 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.1

Urinary hippuric acid was analyzed by gas chromatography/mass spectrometry method. A simple and economical derivatization procedure using excessive methanol in acidic condition was adopted to esterify hippuric acid. Quantitative analysis was performed with two internal standards, heptadecanoic acid and hydrocinnamic acid, to evaluate the derivatization procedure as an analytical method. Using the standard addition method, linear and reproducible calibration curves were obtained for both internal standards. Calibration experiment showed that good quantitative result could also be obtained with the calibration curve obtained without urine matrix. Recovery rates for the urine samples were in the range of 95.6∼111.4%. Using hydrocinnamic acid as internal standard, analysis time could be reduced.

디메틸 실록산과 2-히드록시에틸메타크릴레이트계 블락공중합체의 합성 : 초임계 이산화탄소용 양손잡이형 계면활성제 Ambidextrous Surfactant for Supercritical Carbon Dioxide

황하수,이민영,임권택 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

Block copolymers containing dimethy siloxane and 2-hydroxyethyl methacrylate sequences were synthesized by group transfer polymerization (GTP) of 2- trimethylsilyloxy ethyl methacrylate(TMS-EMA) using silyl ketene acetal terminated poly(dimethylsiloxane) (PDMS) as macroinitiator, followed by hydrolysis of TMS-EMA to HEMA. The block copolymers were obtained with controlled molecular weight and narrow molecular weight distribution. Trimethylsilyl groups in the P(TMS-EMA) block could be selectively hydrolyzed without interfering with Si-O bond in PDMS block. The block copolymers formed micelles in methanol, the effective diameters (Rh) of which were in the range of 78 ~110 nm with narrow distribution by dynamic light scattering (DLS). The latex particles, PMMA was synthesized by dispersion polymerization in supercritical carbon dioxide with these block copolymers as stabilizer.

흉강경을 이용한 폐생검으로 확진된 철폐증

권순하,김양기,이영목,장안수,김기업,고은석,어수택 순천향의학연구소 2010 Journal of Soonchunhyang Medical Science Vol.16 No.1

..

과불소우레탄 공중합체의 합성과 표면특성

김동권,이수복,하종욱,김정훈,박인준 한국공업화학회 2003 응용화학 Vol.7 No.2

Perflurorourethane copolymers were stnthesized by free-radical copolymerization using a fluorine-containing monomer and urethane monomers as starting materials. The fluorine-containing monomer used was perfluoroalkyl alcohol(FOH), and the urethane monomers used were tolylene 2,4-diisocyanate(TDI), hexamethylene diisocyanate(HDI), and 4,4-methylene bis(phenyl isocyanate)(MDI). The surface properties and surface modification ability of the perfluorourethane copolymers prepared were investigated. The surface free energies of polyethylene(PE) films modified by the perfluorourethane copolymers and their surface modifkcation properties were also investigated.

VDT작업자에서 업무로 인한 정신사회적 스트레스에 대한 인지가 근골격계장애에 미치는 영향

백남종,하미나,조수헌,강대희,백도명,주영수,윤덕로,권호장 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

업무에 의한 정신사회적 스트레스가 근골격계 질환의 유병에 미치는 영향을 파악하기 위하여 전화안내원을 대상으로 단면적 조사연구를 수행하였다. 연구 결과 나이, 교육정도, 근무연한, 가사부담 등의 인구학적 변수는 근골격계질환의 유병에 영향을 미치지 않는 것으로 분석되었다. 정신사회적 스트레스 중 업무요구도가 높다고 느끼는 집단에서 그렇지 않은 집단에 비해 근골격계 질환의 유병률이 통계적으로 유의하게 높게 나타났으나 업무재량도나 사회적지지도는 근골격계질환과 유의한 상관관계를 발견할 수 없었다. 그러나 업무요구도는 높고 업무재량도는 낮다고 느끼는 '근무긴장도가 가장 높은 군'은 업무요구도는 낮고 업무재량도는 높다고 느끼는 '근무긴장도가 낮은 군'에 비해 근골격계 질환의 발생위험이 통계적으로 유의하게 높은 것으로 나타났다. 본 연구의 결과는 업무로 인한 정신사회적 스트레스에 대한 인지 정도에 따라 근골격계질환의 유병률이 달라짐을 시사하고 있다. 따라서 작업관련성 근골격계질환의 예방을 위해서는 기존의 인간공학적 접근 이외에 스트레스를 감소시키려는 노력이 동시에 진행되어야 할 것으로 생각한다. A cross-sectional study was conducted to assess the association between perceived occupational psychosocial stress at work and the work-related musculoskeletal disorder(WRMSD) among employees using video display terminals. The study included 111 female telecommunication employees from three companies at ChungBuk province. Cases of WRMSD were defined using symptom questionnaire and physical examination conducted by rehabilitation specialist. Information on demographics, individual factors, and perceived psychosocial stress, were obtained by self-administered questionnaire. For assessing perceived psychosocial stress, we used variables from 'job strain model' proposed by Karasek. Associations between perceived psychosocial stress and WRMSD were assessed by multiple logistic regression models. Forty nine(44% to the total) subjects met our operational definition for WRMSD. Age, seniority, housing load were not associated with WRMSD. Perceived psychosocial stress was associated with WRMSD[odds ratio=3.28, 95% confidence interval: 1.05-10.19]. This study suggests that perceived occupational psychosocial stress is related to the prevalence of WRMSD.

유기용제 사용 소규모사업장의 체계적 건강관리 프로그램 개발에 관한 연구1) : 신경행동학적 검사법의 적용 가능성 Application of Neurobehavioral Core Test Battery(NCTB)

한상환,하미나,권호장,윤덕로,조수헌 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2

It is known that there appear some nonspecific and delicate changes in neuropsychiatric aspect on the workers when they are exposed for a long period to low concentrated organic solvents in the work places. Therefore, it gives rise to the necessity of developing a program for the health supervisor stationed in the work place to supervise these neurobehavioral health affection. In addition to the questionnaire posed to the workers exposed to organic solvents, the study team conducted NCTB(Neurobehavioral Core Test Battery) respectively on an exposed group and a non-exposed group to organic solvents. The study team compared the results and decided whether or not to apply the results to the evaluation program of health affection. The study team divided 132 workers in 6 car repairing factories and 2 textile printing factories into exposed group and non-exposed group, pairing them off into 66 research objects according to their age, sex, and job. Neurobehavioral test was conducted on 6 items, except the POMS test, of NCTB developed by the World Health Organization(WHO). The study team conducted examinations of working environment on the exposed and non-exposed groups of each work place. The analysis of the collected materials for examinations were conducted with GC-MS. The concentration of the mixture in the air, relative to their respective threshold limit values(TLV) was calculated according to the formula of the ACGIH and the Korean threshold limits. The results indicated that for two of eight factories samples the recommended concentration limits for mixtures was exceeded. Of the six tests, the 'digit span forward', 'digit span backward' and 'digit symbol' produced conspicuous statistical differences between the two groups(p<0.1) as a result of compairing the test values by means of paired t-test. The results of respective analysis of the car repairing factories and textile printing factories revealed that the textile printing factories had greater differences between the exposed and the non-exposed than in the car repairing factories. It is presumably because the textile printing workers are continuously exposed to the organic solvents during working hours, whereas the car repairing workers are exposed only when they are doing painting works, The NCTB is assessed very useful in examining the neurobehavioral health affection under the exposure to organic solvents, and, therefore, the study team expects that the NCTB will play an important part in the course of developing a health program for the workers exposed to organic solvents.