아토피 피부염의 중증도에 작용하는 중요인자

장가연,조소연,강호정,함정희 梨花女子大學校 醫科大學 醫科學硏究所 1998 EMJ (Ewha medical journal) Vol.21 No.3

목적: 아토피 피부염의 중증도나 동반 알러지 질환 유무에 따른 임상 및 검사실 소견의 차이와 그 의의를 알아보고자 하였다. 방법: 1995년 8월부터 1996년 7월까지 1년동안 본원 피부과 외래를 방문한 환자를 대상으로 피부과 의사의 직접적인 면담, 신체검사, 세균배양검사, 단자 검사 및 면역학적 검사를 시행하였다. 결과: 1) 피부 건조증, 이개 균열, hyperlinear paim의 동반이 중증도에 따른 유의성을 보였다. 2) 땀, 음식, 흡입항원이 중증군에서 통계학적으로 유의하게 악화요인으로 작용하였다. 3) 아토피 피부염의 가족력 도압ㄴ율이 중증군에서 57.9%, 경증군에서 26.3%로 중증도에 따른 유의성을 보였다. 4) 호흡기 아토피 질환 유무에 따른 집먼지 진드기에 대한 피부단자검사 양성율이 통계학적인 유의성을 보였다. 5) 총 혈청 IgE가 중증군에서 938.3±601.8IU/mL, 경증군에서 526.7±352.1IU/mL로 통계학적인 유의성을 보였다.(p<0.05) 6) 호흡기 아토피 질환이 동반된 아토피 피부염환자의 혈청 IgE는 1025.8±713.2IU/mL, 호흡기 아토피 질환이 동반되지 않은 군은 403.3±273.4IU/mL 로 유의한 차이를 보였다(p<0.05). 7) 혈청 호산구 값은 중증군에서는 509.9±398.4/㎣, 경증군에서는 340.5±219.4/㎣로 중증군에 따른 유의성을 보였다(p<0.05). 결론: 1) 아토피 피부염의 임상적 중증도와 연관있는 것은 피부 건조증, 이개균열, hyperlinear palm 같은 피부 보조증상, 땀, 음식, 흡입항원같은 악화요인이며, 혈청 IgE 증가 및 혈중 호산구증가같은 거사실 소견이었다. 2) 호흡기 아토피 질환이 있는 아토피 피부염 환자에서 높은 혈청 IgE치와 집먼지 진드기에 대한 높은 단자 검사 양성율을 보였다. Objectives : This study was performed to investigate the clinical manifestations and labora-tory findings, and to see whether the severity of atopic dermatitis(AD) implies a relationship to the clinical and laboratory findings. Methods : Our study was designed by analyzing outpatients with AD via physical examinat-ion, questionnaires and laboratory investigations such as prick test to house dust mites, bacterial cultures, total IgE, IgA, IgG, IgG_4, IgM, and peripheral eosinophil count. Results : 1) Xerosis, ear fissuring and hyperlinear palms were related to the severity of AD(p<0.05). 2) Sweating, foods and aeroallrgen were related to the severity of AD(p<0.05). 3) The presence of family history of AD differed significantly(p<0.05) between the severe group(57.9%) & the mild group(26.3%). 4) The positivity of the prick test to Dermatophagoides pteronyssinus and D.farinae was found in 90.0% of respiratory group and in 30.0% of AD only(p<0.05%). 5) Serum IgE level was higher in the severe group(938.3±601.8 IU/mL) than the mild group(526.7±352.1IU/mL)(p<0.05). 6) Serum IgE level in AD patients with respiratory disease(1025.8±713.2IU/mL) was higher than AD only(403.3±273.4IU/mL)(p<0.05). 7) Peripheral eosinophil counts were higher in the severe group(509.9±389.4/㎣) than the mild group(340.5±219.4/㎣)(p<0.05). Conclusion : The factors related to severity of AD were xerosis, ear fissuring, hyperlinear palms, sweating, food, aeroallergen, serum IgE and peripheral eosinophil count. The AD pati-ents with respiratory allergic disease had higher IgE levels and higher positive rates of prick test with house dust mite.

Chinese Hamster Ovary 세포에 발현시킨 유전자재조합 사람 갑상선자극호르몬 수용체를 이용한 갑상선자극호르몬 수용체 항체 측정

조보연,이홍규,김원배,고창순,고경수,정재훈,이가희 대한내분비학회 1995 Endocrinology and metabolism Vol.10 No.4

Thyroid stimulating antibody which results in the development of hyperthyroidism and goiter in Graves' patients used to be measured by using rat thyroid cells, FRTL-5. However, this assay has disadvantages: decreased sensitivity due to differences in species, and fastidious culture conditions for FRTL-5 cells. Thus, we recently created stably transfected Chinese hamster ovary(CHO) cells containing the human TSH receptor(hTSHR-CHO) and developed optimal conditions for the measurement of thyroid stimulating antibody using hTSHR-CHO cells. In this study, to evaluate the clinical relevance of thyroid stimulating antibody measurement using hTSHR-CHO cells, we measured thyroid stimulating antibody activities of IgGs from Graves' disease and other thyroid disease using hTSHR-CHO cells, and compared to those of thyroid stimulating antibody assays using FRTL-5 cells. 1) The cut off value of positive thyroid stimulating antibody activity measured in hTSHR-CHO cells was 145%(above the mean +2SD) which was lower than 165% in FRTL-5 cells. The intra-assay and inter-assay variances were 3.9% to 9.0% and 12.7% to 1.6%, respectively. 2) Thyroid stimulating antibody activity was detected in 90% of patients with untreated Graves' disease when patients initially presented. Further, in patients seen initially but already under therapy, 75% had positive values if they were hyperthyroid but only 43% had IgGs with activity if they were euthyroid. Patients in clinical remission after therapy showed positive values in 23% of cases. Only 2 of 25 patients with Hashimoto's thyroiditis showed weak thyroid stimulating antibody activity, none of 18 patients with nodular nontoxie goiter, 1 of 15 patients with primary myxedema, and 2 of 33 control patients with no thyroid disease. Thus, the detection frequency and specificity of the assay with hTSHR-CHO cells was excellent for this type bioassay. 3) The detection frequency of thyroid stimulating antibody activity by hTSHR-CHO cells assay system(90%) was higher than that by FRTL-5 cells assay system(66%) in untreated Graves' patients. Those two activities were positively correlated with each other(r=0.52, p$lt;0.001). However, some IgGs showed discrepancy of the thyroid stimulating antibody activity measured in hTSHR-CHO cells and in FRTL-5 cells; 56 of 87 patients were positive in both cells system, 8 of 87 were negative in both cells system, 1 of 87 was only positive in FRTL-5 cells and 22 of 87 were only positive in hTSHR-CHO cell system. Thus, 73%(22/30) of IgGs showing negative values of thyroid stimulating antibody activities in FRTL-5 cells were detected its activities in hTSHR-CHO cells system. In summary, thyroid stimulating antibody assay with hTSHR-CHO cells exhibited so excellent sensitivity and specificity that this technique should be used for clinical practice as well as basic research(J Kor Soc Endocrinol 10: 347-361, 1995).

사람 갑상선자극호르몬 수용체를 발현하는 Chinese Hamster Ovary 세포를 이용한 갑상선자극항체 활성의 측정 : 측정 조건의 최적화 Optimization of Assay Condition

송영기,조보연,이홍규,김원배,고창순,오연상,이가희 대한내분비학회 1995 Endocrinology and metabolism Vol.10 No.4

We investigated the optimal condition of thyroid stimulating antibody(TSAb) assay using Chinese hamster ovary cells transfected with cDNA of human TSH receptor(TSHr-CHO) stably expressing functional TSH receptors. The extracellular cAMP responses of TSHr-CHO cells to the stimulation of bTSH or Graves' IgG were observed in three different incubation media. Stimulation indices of extracellular cAMP were higher when sucrose containing NaCl-free isotonic Hank's balanced salt solution(HBSS)(media A)was used as incubation media than those of NaCl-free hypotonic HBSS(media B) or those of NaCl containing isotonic HBSS(media C). The incubation of TSHr-CHO cells in media B caused marked increase in the basal cAMP level without concomittant fold-increase in the stimulated cAMP level at various doses of bTSH and Graves' IgG. Decreasing the stimulation indices of extracellular cAMP, use of media B failed to detect TSAb activities in two TSAb-positive Graves' IgG tested. In case of media C, extracellular cAMP responses are poor at 0.001 and 0.1U/L of bTSH and at all doses of Graves' IgG tested(0.5, 1, 5g/L). The incubation of TSHr-CHO cells in media B caused significant increase in the number of trypan blue-stained, nonviable cells(5.7+-1.5, 7.6+-1.9 and 8.5+-1.6% at 1, 2 and 3h of incubation, respectively; p<0.01) comparing to those incubated in media A or media C(about 2-3% in both media). Those decrease in the viability of TSHr-CHO cells when incubated in hypotonic incubation media may explain the decrease in the stimulation index of extracellular cAMP with the use of media B in contrast to the case of FRTL-5 cells. TSAb assay of 87 consecutive fresh Graves' patients with TSHr-CHO cells using media A detected TSAb activities in 90%(78 patients) of them, and moreover TSAb activities showed significant positive correlation with the pre-treatment serum T_3 and free T_4 levels of those patients. We conclude that TSAb assay with TSHr-CHO cells is a sensitive and physiologically relevant assay system to measure TSAb activities merely through measurements of extracellular cAMP provided that the cells are incubated in NaCl-free isotonic incubation media(J Kor Soc Endocrinol 10: 333-346, 1995).

An optimal standardized in vitro bioassay to evaluate susceptibility of green peach aphid, Myzus persicae ( Sulzer) ( Insecta: H emoptera: A phididae), t o aphicides

Ka Hee Cho,Hyo Jung Kim,Yu Na Kim,Young Cheol Kim 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

Leaf-spray in vitro bioassays appraise new aphicidal formulations for managing deleterious plant-feeding aphids. The formulation may utilize alternative and integrated strategies. However, leaf spraying even under controlled conditions may affect aphid reproduction and mortality. This study examines leaf spray applications for optimum and reproducible aphicidal results using tobacco leaves overlaid on cotton fabric or water agar surfaces. Infestation of the undersides of tobacco leaves with nymphs of green peach aphids was used in the assays. Spray distance and volume were optimized using water-sensitive paper to ascertain the best surface coverage. Overlays of the leaves on water agar caused less mortality and greater reproduction than the use of cotton fabric. The relative humidity of the insect-rearing chambers changed with the watering regime for the insect - rearing chambers with cotton fabric; 60% relative humidity was optimal. Relative humidity was not affected by the concentration of agar in the water agar chambers. Applications of the chemical aphicidal standard, Sulfoxaflor, under the optimized conditions exhibited similar times for lethality although the rate was faster with leaves on the cotton fabric than on water agar. These studies establish reproducible and sensitive techniques for assessing the lethality and effects on reproduction of potential aphicidal products.

Determination of lethal concentrations and lethal times of extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens, to control green peach aphid, Myzus persicae

Ka Hee Cho,Hyo Jung Kim,Song Hee Han,Yu Na Kim,Young Cheol Kim 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.10

Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of active insecticidal compounds (Ais) in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of Ais, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan - Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

Pathogenic Analysis of Mouse-Adapted 2009 Influenza A (H1N1) Mutant Virus

Ka Hee Cho,Min Kyung Yoo,Min Ah Park,Eun Jung Park,Ji Sun Oh,Jae Jin Chang,Jang Hoon Choi,Joo Yeon Lee,Chun Kang,Wan Je Park 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8

복막투석 : 자동복막투석의 지속성 복막투석과 비교한 임상적 특징

조진희 ( Jin Hee Cho ),가진희 ( Jin Hee Ka ),이문영 ( Moon Young Lee ),유수경 ( Su Kyong Yu ),박인휘 ( In Whee Park ),김흥수 ( Heung Soo Kim ),신규태 ( Gyu Tae Shin ) 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1

Amiodarone 에 의해 유발된 갑상선 중독증 1 예

조보연,이홍규,고창순,민헌기,송민호,이가희,최윤식 대한내과학회 1991 대한내과학회지 Vol.41 No.6

Amiodarone, a class III antiarrhythmic drug containing iodine, is widely used for treatment of liftthreatening refractory cardiac arrthmias in Korea, The drug has a variety of complex effects on thyroid hormone physiology and occasionally causes clinically overt hyperthyroidism and hypothyroidism. Recently we experienced a case of thyrotoxicosis induced by lowdose amiodarone therapy for control of paroxysmal supraventricular tachycardia due to Wolff-Parkinson-White syndrome that went into euthyroid remission on withdrawal of the drug. We presents a case of amiodarone-induced thyrotoxicosis, firstly reported in Korea, with literature review.

Isolation and Characterization of 2-Methyl-4-Chlorophenoxyacetic Acid-Degrading Bacteria from Agricultural Soils

Cho, Seung-Hee,Ka, Jong-Ok 한국응용생명화학회 1999 Journal of Applied Biological Chemistry (J. Appl. Vol.42 No.2

Seven numerically dominant 2-methyl-4-chlorophenoxyacetic acid (MCPA)-degrading bacteria were isolated from agricultural soils. The isolates utilized the herbicide MCPA as a sole carbon source, producing significant biomass in MCPA mineral medium. They exhibited diverse herbicide degradation capabilities, but most of them grew very slowly in mineral medium containing herbicide. The chromosomal DNA patterns of the isolates obtained by polymerase chain reaction amplification of repetitive extragenic palindromic sequences were distinct from each other. One isolate, SH3, which was identified as Sphingomonas species by fatty acid methyl ester analysis, was able to degrade 5 different phenoxyacetic acid herbicides within 4 days. This strain contains two plasmids, and the smaller one has a crucial role in herbicide degradation. MCPA treated into agricultural soils without indigenous MCPA-degraders persisted for a long time, but the application of the isolate SH3 resulted in rapid decline of MCPA concentration in the soil.

Cinnamaldehyde induces apoptosis by ROS-mediated mitochondrial permeability transition in human promyelocytic leukemia HL-60 cells

Ka, Hyeon,Park, Hee-Juhn,Jung, Hyun-Ju,Choi, Jong-Won,Cho, Kyu-Seok,Ha, Joohun,Lee, Kyung-Tae WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Cinnamaldehyde is an active compound isolated from the stem bark of Cinnamomum cassia, a traditional oriental medicinal herb, which has been shown to inhibit tumor cell proliferation. In this study, we investigated the effects of cinnamaldehyde on the cytotoxicity, induction of apotosis and the putative pathways of its actions in human promyelocytic leukemia cells. Using apoptosis analysis, measurement of reactive oxygen species (ROS), and assessment of mitochondrial membrane potentials (ΔΨ_(m)), we show that cinnamaldehyde is a potent Inducer of apoptosis and that it transduces the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT) and cytochrome c release to the cytosol. ROS production, mitochondrial alteration, and subsequent apoptotic cell death in cinnamaldehyde-treated cells were blocked by the antioxidant N-acetylcystein. Taken together, our data indicate that cinnamaldehyde induces the ROS-mediated mitochondrial permeability transition and resultant cytochrome c release. This is the first report on the mechanism of the anticancer effect of cinnamaldehyde.