젖소 말초혈액 림프구로부터 소백혈병 바이러스 배양 및 전자현미경적 관찰

윤순식,박중원,변재원,강문일,유한상,한홍율 韓國電子顯微鏡學會 2005 Applied microscopy Vol.35 No.1

국내 젖소의 54.2%가 BLV에 감염되어 있지만 현재까지 국내에서는 소백혈병 바이러스 (BLV) 입자를 확인한 연구 보고가 없기 때문에 BLV 항체 양성 소의 말초혈액림프구를 배양, BLV를 발현시켜 전자현미경으로 바이러스 입자를 검출하였고 배양조건에 따른 바이러스 발현율 및 발현 시간을 비교하였다. 검사 결과 전형적인 C-형 바이러스를 확인할 수 있었으며 BLV 단크론 항체를 이용한 면역염색결과 BLV 항원 양성으로 확인되었다. BLV 는 대부분 세포 외부에 분포하고 있었으며 세포질 막에서 생성, 발아되어 나오는 것도 관찰되었다. 전체 바이러스의 크기는 90~100 nm였으며 nucleocapsid는 40~60nm였다. 소태아혈청 (FBS)과 T- 및 B-림프구 분열촉진물질(mitogen)을 각각 첨가하여 배양한 결과 두 군 모두에서 BLV 발현이 확인되었다. Lipopolysaccharide 첨가군은 배양 12시간, Conconavalin A 첨가군은 배양 24시간에 각각 림프구의 10%에서 바이러스가 관찰되었다. 또한 FBS만 첨가한 군과 FBS와 mitogen을 모두 첨가하지 않은 군에서도 관찰되었으나 바이러스의 수는 적었다. 본 연구에서 확립된 BLV 배양 기법을 활용하면 BLV에 감염된 소 중 바이러스를 발현하는 소, 즉 전파능이 있는 개체를 찾아내어 우선적으로 도태할 수 있기 때문에 BLV 감염으로 인한 피해를 막는데 효과적으로 이용될 수 있을 것으로 사료된다. Many studies have been performed on the bovine leukemia virus (BLV) since bovine leukosis had been reported in 1968 in Korea. However, there was no report on the ultrastructural examination of BLV. An attempt to detect C-type viral particles in the cultured peripheral blood lymphocytes of Holstein-Friesian dairy cattle, was made to determine whether in vitro viral expression might be used as a reliable method to identify the cow which is likely to transmit BLV. In transmission electron microscopic (TEM) examination, the virus particles were found predominantly outside of the lymphocytes even though a few particles were also observed within the membrane bound cytoplasmic vacuoles. All of them were C-type particles consisting of a central, electron-dense core separated by a clear area from a limiting envelope with a unit membrane structure. Virus particles were easily detected in the lymphocyte which was cultured with medium supplemented with either T-lymphocyte mitogen (conconavalin A) or B-lymphocyte mitogen (lipopolysaccharide). Identical viral particles, although fewer, were also consistently present in the lymphocytes cultured with medium which was containing foetal bovine serum (FBS) only and which was containing neither FBS or mitogen. By contrast, no virus particle was detected in extensive examination of lymphocytes before culture. In conclusion, the BLV cultivation and detection methods established in this study could be used as a tool to identify and eliminate the cattle which can transmit the BLV.

Neuroprotective Effect of Compound From Brown Alga Eisenia bicyclis on Amyloid Beta Peptide-Induced Toxicity in Neuron Cells

Jung Kwon Lee,Yong-Jae Kim,Han-Il Seo,Ryul-Rok Bahn,Hee-Guk Byun 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Peripheral Blood Inflammatory Cytokines in Idiopathic REM Sleep Behavior Disorder

Kim, Ryul,Jun, Jin‐,Sun,Kim, Han‐,Joon,Jung, Ki‐,Young,Shin, Yong‐,Won,Yang, Tae‐,Won,Kim, Keun Tae,Kim, Tae‐,Joon,Byun, Jung‐,Ick,Sunwoo, Jun‐,Sang,Jeo John WileySons, Inc. 2019 Movement disorders Vol.34 No.11

<P><B>Abstract</B></P><P><B>Background</B></P><P>Although previous research provides insight into the role of neuroinflammation in idiopathic REM sleep behavior disorder, the association of this disorder with peripheral blood inflammatory markers remains unclear.</P><P><B>Objective</B></P><P>To investigate inflammatory cytokines in plasma samples in patients with idiopathic rapid eye movement sleep behavior disorder and to explore whether these markers are associated with prodromal symptoms of α‐synucleinopathies.</P><P><B>Methods</B></P><P>We collected plasma from patients with polysomnographically confirmed idiopathic rapid eye movement sleep behavior disorder without parkinsonism or dementia (n = 54) and from healthy controls (n = 56). The following cytokines were measured: interleukin‐1β, interleukin‐2, interleukin‐6, interleukin‐10, and tumor necrosis factor‐α. The idiopathic REM sleep behavior disorder patients underwent sleep, motor, cognitive, olfactory, and autonomic testing.</P><P><B>Results</B></P><P>The anti‐inflammatory cytokine, interleukin‐10, levels in the idiopathic rapid eye movement sleep behavior disorder group were significantly upregulated compared to the control group (<I>P</I> = 0.022), but this difference did not withstand Bonferroni correction. The other proinflammatory cytokine levels did not differ between the groups. No correlation was found between the cytokine levels and any clinical variable.</P><P><B>Conclusions</B></P><P>Our data do not provide evidence supporting the role of peripheral inflammation in idiopathic rapid eye movement sleep behavior disorder. However, considering the limited statistical power because of the small sample size, further large‐scale longitudinal studies with a broader spectrum of cytokines are needed to clarify this issue. © 2019 International Parkinson and Movement Disorder Society</P>

제1기 비소세포폐암에서 APT1 발현의 임상적 의미

신정아 ( Jung Ar Shin ),이창률 ( Chang Ryul Lee ),변민광 ( Min Kwang Byun ),장윤수 ( Yoon Soo Chang ),김세규 ( Se Kyu Kim ),장준 ( Joon Chang ),안철민 ( Chul Min Ahn ),김형중 ( Hyung Jung Kim ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.4

Background: Acyl protein thioesterase-1 (APT1) is a cytosolic protein that may function in the depalmitoylation of numerous proteins, including the Ras family. However, the clinical role of depalmitoyl thioesterase in human cancer is not known. We evaluated the APT1 expression in lung cancer tissue and its clinicopathological findings according APT1 expression pattern. Methods: APT1 expression was examined by immunohistochemistry in the tumor tissue from 79 patients, who had undergone curative surgical removal of the primary lesion; all patients had been diagnosed with stage I non-small cell lung cancer between 1993 and 2004, at Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. Results: The APT1 expression was seen in 50 out of 79 (63.3%) cases. The positive APT1 expression was significantly related with histologic subtype and T stage, but was not influenced by differentiation. The positive APT1 expression was not significantly related to patient age, gender, or smoking history. The median follow-up duration was 10.0 years; the 5-year survival rate was 71.0%. The positive APT1 expression group showed significantly worse overall survival and worse disease-free survival without statistical significance. Conclusion: We conclude that positive APT1 expression in stage I lung cancer after surgery is closely associated with overall survival. To evaluate APT1 as a prognostic marker in lung cancer, comprehensive studies on advanced stage cases are needed.

“재미” 감성 주제 온톨로지를 이용한 질의어 확장 멀티미디어 데이터 검색 시스템 구현

이정송(Jung Song Lee),변동률(Dong Ryul Byun),박순철(Soon Cheol Park) 한국정보과학회 2010 한국정보과학회 학술발표논문집 Vol.37 No.1C

최근 컴퓨터와 네트워크의 기술 발달로 멀티미디어 데이터가 폭발적으로 증가하고 있다. 따라서 정보 검색 시스템도 텍스트 데이터 위주에서 벗어나 멀티미디어 데이터 검색이 큰 비중을 차지하고 있다. 또한 멀티미디어 데이터 질의어처리도 기술적인 변화와 함께 다양한 질의어 확장으로 검색의 정확성을 높이고 있다. 본 논문에서는 인간의 감성에 대한 ‘재미’ 주제 온톨로지를 구축하여 질의어 확장에 응용하였고, 한편의 동영상에서 재미 요소를 찾아내는 멀티미디어 데이터 검색 시스템을 구축하였다. 온톨로지 구축은 한글 워드넷(KorLex)에서 “재미”라는 특정 감소 요소의 의미 계층 구조를 파악하고 토픽맵을 이용하여 구축하였다. 또한, 온톨로지에 정의된 용어들 사이의 가중치는 실시간으로 계산하여 질의어를 확장에 적용하였으며, 따라서 검색의 효율성과 질을 높였다. 검색방법은 사용자가 질의어를 직접 입력하는 텍스트 입력검색과 온톨로지 구조를 이용한 GUI 인터페이스 검색방법으로 나누어 사용자의 편의성을 증대시켰다.

난관수종액이 초기 임신 과정에 태반 세포 증식에 미치는 영향

김정욱,김계현,이지애,한정렬,최범채,변혜경,유근재,궁미경,조셉 힐 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.8

Objective : To assess the effect of hydrosalpingeal fluid on trophoblast cell proliferation and evaluate the cytokine level in hydrosalpingeal fluid Materials and Methods : Hydrosalpingeal fluid samples obtained from 10 women at the time of laparoscopic surgery. Trophoblast cell proliferation was assayed by a colorimetric immunoassay, based on the measurement of BrdU incorporation during DNA synthesis in response to hydrosalpingeal fluid and the levels of the Th-1 and Th-2 type cytokine determined by sandwich ELISA. Statistical analysis was performed by regression analysis and nonparametric testing(Mann-Whitney U test). Results : Samples from 7 out of 10 patients significantly suppressed trophoblast proliferation in a dose- dependent manner compared to controls(r=-0.0673, p$lt;0.05). IFN-γ and IL-6 were present in 2 out of 10 hydrosalpingeal fluid samples. IL-10 was not detected in all 10 samples. TNF-α, Th-1 type cytokine, was checked in all samples [median(range); 13.12 pg/ml(8.27 pg/ml~19.67 pg/ml)], and the mean concentration of TNF-α was significantly greater in group that suppressed trophoblast proliferation than in group that did not(14.74 pg/ml ± 1.05 pg/ml versus 9.33 pg/ml ± 0.55 pg/ml, p=0.0164). Conclusions : These findings suggest that hydrosalpingeal fluid may negatively affect trophoblast cell proliferation leading to implantation failure and also high level of the Th-1 type cytokine, TNF-α, in hydrosalpingeal fluid may be a potential mechanism for early pregnancy loss.

주요 전기 기기에 대한 각국의 에너지효율기준 비교 분석

원종률(Jong-Ryul Won),김정훈(Jung-Hoon Kim),변천석(Chun-Seok Byun),서백호(Baek-Ho Seo) 대한전기학회 2005 대한전기학회 학술대회 논문집 Vol.2005 No.7

데이터방송 인터랙티브 게임 규제 및 이용자 인식에 관한 연구

변동현(Dong-Hyun Byun),정문열(Moon-Ryul Jung),배홍섭(Hong-Seob Bae) 한국언론정보학회 2006 한국언론정보학보 Vol.35 No.-

본 논문은 유럽 등 해외에서 현재 활성화 되어있는 카드게임, 경마게임 등을 활용한 데이터방송 승부게임(TV-betting) 서비스를 조작하여 이를 경험한 패널집단을 대상으로 인터랙티브 게임 관련 긍정적, 부정적 인식을 조사하여 국내에서 데이터방송 승부게임에 대한 규제문제와 그 도입가능성을 모색해 보았다. 조사연구를 수행하기 위해 국내 디지털케이블방송에서의 데이터방송 표준인 OCAP시스템 환경에서 데이터방송 승부게임 데모프로그램을 제작하고 패널집단이 이 프로그램의 시청경험을 하게 한 뒤에 심층적인 면접을 시행하였다. 그 결과를 정리하면 다음과 같다. 패널집단은 데이터방송 승부게임이 오락효과, 감정정화, 스트레스 해소 등 시청자에게 즐거움을 주는 긍정적 요소가 많으나 사회적 환경 때문에 외국과 같이 쉽게 도입되기는 어려울 것으로 인식하고 있었다. 그리고 데이터방송 승부게임 이 도입되기 위해서는 결제에서의 인정된 보안장치를 기반으로 매체 신뢰도가 보장 되어야 하고, 승부게임을 너무 자주하지 않게끔 시간적 제약을 고려한 방송편성, 베팅(betting)한도의 제한, 다른 데이터방송 가입자들의 셋탑박스에 접속하여 게임을 하는 경우를 차단하는 등 일련의 규제적 시스템 장치를 통해서 과도한 사행성을 억제해야 한다고 판단하고 있었다. 이에 여러 가지 부작용을 최소화하는 방안 등 정부의 법적인 규제 틀 안에서 승부게임의 단계적인 도입이 검토될 수 있다고 인식하고 있었다. 정부는 아를 고려해 장기적인 관점에서 데이터방송 정책수립을 해야 하고, 이를 통해 법적인 규제 틀 안에서 안정적으로 데이터방송 승부게임을 즐길 수 있는 방안이 마련된다면 향후 국내에서 인터랙티브 TV게임이 오락적인 기능과 함께 산업적으로는 데이터방송 등 뉴미디어 산업 활성화를 도모할 수 있을 것으로 기대된다. This study examines the regulatory issues and introduction problems of TV -betting data broadcasts in Korea by in-depth interview with a panel group. TV -betting data broadcast services of card games and horse racing games ate widely in use in Europe and other parts of the world. In order to carry out the study, a demo program of TV-betting data broadcast in the OCAP(OpenCableTM Application Platform Specification) system environment, which is the data broadcasting standard for digital cable broadcasts in Korea was exposed to the panel group and then they were interviewed after watching and using the program. The results could be summarized as below. First of all, while TV-betting data broadcasts have many elements of entertainment, the respondents thought that it would be difficult to introduce TV-betting in data broadcasts as in overseas countries largely due to social factors. In addition, in order to introduce TV-betting data broadcasts, they suggested that excessive speculativeness must be suppressed through a series of regulatory system devices, such as by guaranteeing credibility of the media based on safe security systems for transactions, scheduling programs with effective time constraints to prevent the games from running too frequently, limiting the betting values, and by prohibiting access to games through set-top boxes of other data broadcast subscribers. The general consensus was that TV-betting could be considered for gradual introduction within the governmental laws and regulations that would minimize its ill effects. Therefore, the government should formulate long-term regulations and policies for data broadcasts. Once the groundwork is laid for safe introduction of TV-betting on data broadcasts within the boundary of laws and regulations, interactive TV games are expected to be introduced in Korea not only for added functionality of entertainment but also for far-ranging development of data broadcast and new media industries.

Original Articles : Antimicrobial resistance of Escherichia coli strains isolated from pigs and their farm environment in Korea

( Suk Kyung Lim ),( Jung Ryul Byun ),( Hee Soo Lee ),( Dong Chan Moon ),( Geum Chan Jang ),( Suk Chan Jung ) 한국예방수의학회 2014 예방수의학회지 Vol.38 No.3

The aim of this study was to investigate the prevalence of antimicrobial resistance in Escherichia coli isolated from pigs and their farmhouse environment in Korea. A total of 585 E. coli were isolated in this study during 2006∼2007: 426 isolates from 492 pigs and 159 isolates from 312 farmhouse environment samples from 16 different pig farms. The most frequently observed resistance in pig fecal samples was to older antimicrobials such as tetracycline, streptomycin, and ampicillin. However, resistance to cephalosproins, β-lactam/ β-lactam inhibitor combination, and colistin was low. We found an inverse relationship between prevalence of resistant E. coli and animal age. Resistance rate and multi-drug resistance (MDR) was greater in young pigs (piglet and nursery) than for those from adult pigs (grower-finish and sow). Resistance of E. coli from farm environment such as floor, Iron partition, and ventilation was similar with those from pig fecal samples. Farm environment contaminated with MDR bacteria might be a possible source of infections to pigs. Thus, to control and reduce the antimicrobial resistance in pigs, we must also pay attention on the environment.

{Poster Presentation} P8 : Electronmicroscopic Study of Bovine Leukemia Virus of Cattle

( Soon Seek Yoon ),( Jung Won Park ),( Jae Won Byun ),( Mun Ll Kang ),( Han Sang Yool ),( Hong Ryul Han ) 한국수의병리학회 2005 한국수의병리학회 국제심포지움자료집 Vol.2005 No.-