제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

慶北地方山羊에 發生한 Anaplasmosis

孫濟英,李鉉凡,張仁浩 慶北大學校 1965 論文集 Vol.9 No.-

1) Four goats which had shown anemia, emaciation, nasal discharge and cough for a long time were splenectomized and their blood were examined thereafter. In the results Anaplasma-like bodies were detected in their circulating red blood cells, and the anemia became more marked after the splenectomy. 2) In dry fixed preparations stained with Giemsa, the Anaplasma-like bodies were appeared as minute spherical points measuring from 0.3 to 1.1 micron in diameter, located usually near the margin or rarely center of red blood cells, stained deeply and took red or reddish violet colour. Ordinarily there was only one body per cell although 2 or 3 were seen in some cells. Most of the bodies were spherical in shape. Besides the typical deeply and uniformly staining spherical form, diad, triad or ring forms were occasionally observed in some cells. These findings were coincident with the morphology of Anaplasma described by previous workers. 3) Two healthy goats, a healthy sheep and a splenectomized co w infected with small-type piroplasma were artificially inoculated with the blood of the goats infected naturally with the bodies. In the results it was found that the goats and sheep were appearantly infected with the same boides, whereas the cow did not show any signs of infection. These results seemed to be coincident with the pathogenecity of Anaplasma ovis described by previous workers. 4) From the standpoint of the morphology and pathogenecity of the bodies observed in the present study, it may be concluded that the bodies are Anaplasma ovis. This is the first report of anaplasmosis of goats in Korea.

아세톤 함유 상아질 접착제의 습윤 상아질에 대한 전단 결합강도

손호현,김경남,이광원 大韓齒科器材學會 1994 대한치과재료학회지 Vol.21 No.2

The purpose of this study was to investigate whether or not the wetting of dentin compared to the dried dentin influences the shear bond strength of strong, hydrophilic dentin bonding systems particularly containing acetone. Three dentin bonding systems (i.e. All Bond 2, Mirage ABC, Tenure S) with primers possessing strongly hydrophilic properties were applied to air-dried and moist dentin individually. After the shear bond strength of three dentin bonding systems were evaluated, and then the sheared specimens were examined by light optical and scanning electron microscopy(Hitachi, Japan) to determine the location and appearance of the fracture. The following results were obtained. 1. The enhancement of shear bond strength to moist dentin compared to air dried dentin existed within groups treated with All Bond 2. (p<0.001) 2. A statistically significant difference of shear bond strength did not exist between moist dentin and air-dried dentin within groups treated with Mirage ABC and Tenure S.(p>0.05) 3. The deep cohesive fracture of dentin was appeared within all the moistened specimens treated with All Bond 2. On the contrary, the air-dried specimen S did not show any cohesive fracture of dentin except one. 4. All the moist and dry specimens treated with Mirage ABC and Tenure S did not show any cohesive fracture of dentin.

산부식후 상아질 표면의 습윤 또는 건조가 상아질 결합에 미치는 영향

손호현,권혁춘,양원경 大韓齒科保存學會 2000 Restorative Dentistry & Endodontics Vol.25 No.2

"Clearfil FⅡ" 複合레진에 對한 家犬齒髓의 反應

孫鎬賢 全北大學校 齒醫學硏究所 1984 전북치대논문집 Vol.2 No.1

The biocompatibility of Clearfil FII to the dog's pulp tissue was studied with 60 teeth from 6 dogs. As control materials, zinc-oxide eugenol and silicate cement were used. Class V cavities were prepared and filled with each material. After 3 days, 4, and 8 weeks, the teeth were processed routinly and stained with hematoxylin and eosin. Histopathological tissue changes were observed by light microscope. Following were the results : 1. In the group of zinc-oxide eugenol, slight tissue changes were observed in 4 week cases. In 8 week cases, the pulp tissue was normalized. 2. In the group of unlined silicate cement, the pulp tissue was more severely changed according to the days elapsed. 3. In the group of Clearfil FII, 1 of 10 cases showed moderate pulp tissue change, 6 cases slight change, and 3 cases normal pulp tissue in 4 week cases. In 8 week cases, 2 0f 10 cases showed slight pulp tissue change and the rest showed normal pulp tissue. 4. Clearfil FII was less biocompatible to the pulp tissue than zinc-oxide eugenol, but much more biocompatible than unlined silicate cement.

근관충전방법이 근관폐쇄성에 미치는 영향

손호현 全北大學校 齒醫學硏究所 1983 전북치대논문집 Vol.1 No.1

Conventional canal preparation followed by lateral condensation and step-back canal preparation followed by vertical condensation were performed in the mesiobuccal and mesioligual canals of 50 extracted lower molars. Radiognaphic densities and obturation status using transparent teeth were evaluatec by 5 dentists. The following results were obtained. 1. The guality of canal obturation by vertical condensation was superior to that by lateral condensation. 2. In finely curved conals, step-back preparation resulted in good sealibility. 3. When canals were prepared by conventional method and were condensed laterally, the guality of canal obturation at the apical third was superior to when canals were vertically candensed. 4. At the coronal third of canal, the guality of canal obturation by vertical condensation to that by lateral condensation.

복합례진 충전시 와연우각 각도변화와 산부식이 변연누출에 미치는 영향

손호현 全北大學校 齒醫學硏究所 1985 전북치대논문집 Vol.3 No.1

This study was performed to evaluate the effect of carvosurface angle and acid-etching on marginal leakage. ClassV cavities were prepared on the cervical 1/3 of buccal and lingual surface of freshly extracted human posterior teeth. 40 posterior teeth were divided into 4 group. A group had butt joint as control, B group were beveled at 30 degree, C group 45 degree, and D group 60 degree. In each group, 5 teeth were acid-etched and were applicated with bonding resin and Clearfil Sc composite resin were inserted. Remaining 5 teeth in each group were not acid-etched and Clearfil Sc composite resin were inserted directely into the cavities. All teeth were thermocycled with usual method and were soaked in 2% aqueous methylene blue for 24 hours at 37℃. All teeth were sectioned mesiodistally through the center of teeth and each segment was sectioned again buccolingually to divide the classV cavity restoration into 3 parts equally. Dye penetration were examined on the sectioned surface. The results were as follows : 1. Carvosurface angle design had no significant effect on the occlusal marginal leakage. 2. Microleakage on the occlusal margin was significantly reduced after acidetching and bonding resin application, and microleakage on the gingival margin was reduced some degree but not so much reduced than occlusal margin. 3. Microleakage on the occlusal margin was less than microleakage on gingival margin regardless of acid-etching and bonding resin application. 4. There is no significant difference between control and experimental group. 5. Other factors such as thickness of remaining enamel may be more important in choosing a design of carvosurface margin for a composite resin restoration.

치근단 절제술 시행 2년 후의 치근단 병소 치유 증례

손호현,박정기,최대우 全北大學校 齒醫學硏究所 1985 전북치대논문집 Vol.3 No.1

A 27-year-old Korean woman presented with intermittent dull pain at the periapical area of the maxillary right lateral incisor. At the periapex of the tooth radiolucency was observed. Clinical examination revealed the open pulp chamber, foul, odor, slight mobility and tenderness to percussion. Diagnosis was made as periapical granuloma secondary to pulp necrosis. Conventional root canal therapy was done and the canal were sealed permanently with gutta-percha and zinc oxide-eugenol sealer and finally apicoectomy was performed. Twenty-two months later, standard periapical film of the tooth showed the increasing periapical radiopacity suggesting bon regeneration. Any clinical symptoms were abscent and the maxillary right lateral incisor functioned normally.

In vivo에서 상아질 접착제 도포가 상아세관액 이동과 치수신경활동에 미치는 영향

손호현,이광원,박수정 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.1