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Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
A Study on the Multiple FACTS Control for Ensuring the Voltage Stability in Jeju Island System
Kang, Sang-Gyun,Bae, Moonsung,Han, Youngsun,Yoon, Dong-Hee The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.5
<P>Voltage instability results from a lack of reactive power support in a power system. One effective solution for supplying reactive power to a power system is utilization of flexible alternating current transmission system (FACTS) facilities. Currently, two FACTS facilities are operated for stable operation of the power system on Jeju Island in South Korea. Both FACTS respond to disturbances to stabilize voltage fluctuations in the island power system, however there is potential for mutual interference between them because they are operated using measured voltage without a coherent system operation strategy; cooperative control between the two would result in more effective system operation. Here, a multiple FACTS control algorithm is developed for effective operation of the island power system. The algorithm is based on two methods: calculation of the effective reactive power (Q) reserve (EQR) to obtain an accurate reactive power for the system, and GV analysis to account for the two HVDC interconnections between Jeju Island and the Korean Peninsula.</P>
Drying Characteristics of Sea Tangle Using Combination of Microwave and Far-Infrared Dryer
Han, Chung-Su,Kang, Tae-Hwan,Lee, Jeong-Hyeon,Won, Jin-Ho,Cho, Byeong-Hyo,Cho, Sung-Chan Korean Society for Agricultural Machinery 2016 바이오시스템공학 Vol.41 No.1
Purpose: The present study is aimed at examining the drying characteristics of sea tangle through a combination of microwave and far-infrared drying experiment and finding the optimal drying conditions. Methods: Sea tangle was cleaned and cut into fine pieces (5mm) before they were subjected to combinational drying by microwave and far-infrared ray. The amount of specimen per drying is 2 kg. The finely cut pieces of sea tangle were preheated in a microwave dryer for three different lengths of time (10, 15, and 20 min). Subsequently, they were dried using a far-infrared dryer at tow temperatures ($90^{\circ}C$ and $100^{\circ}C$) at an air velocity of 0.8 m/s until the final moisture content reduced to 10%. Results: Sea tangle dried under the condition of 20 min of preheating in the microwave dryer and drying at $100^{\circ}C$ by the far-infrared dryer. Of the drying models verified in this study, the logarithmic model showed high accuracy with the coefficient of determination $R^2>0.7825$ and RMSE<0.1095. The rehydration ratio of sea tangle was the highest (12.87 g water/g dry matter) under the condition of 15 min of preheating in the microwave dryer and drying at $100^{\circ}C$ by the far-infrared dryer. The energy consumption for the combination of microwave and far-infrared drying was the lowest (4.78 kJ/kg water) under the condition of 20 min of preheating in the microwave dryer and drying at $100^{\circ}C$ by the far-infrared dryer. Conclusions: Considering the drying time, discoloration during drying, rehydration ratio, and energy consumption for the drying of sea tangle, the optimal drying conditions for high-quality sea tangle are 15 min of preheating in a microwave dryer and drying at $100^{\circ}C$ by a far-infrared dryer.
DIGITAL HOMOLOGY GROUPS OF DIGITAL WEDGE SUMS
Kang, Jeang Min,Han, Sang-Eon The Honam Mathematical Society 2016 호남수학학술지 Vol.38 No.4
The present paper investigates some properties of the digital homology in [1, 4, 5] and points out some unclearness of the definition of a digital homology and further, suggests a suitable form of a digital homology. Finally, we calculate a digital homology group and a relative digital homology group of some digital wedge sums. Finally, the paper corrects some errors in [6]. In the present paper all digital images (X, k) are assumed to be non-empty and k-connected.
Induction of Ovulation by Hormone Treatment in Ussurian Bullhead Leiocassis ussuriensis
Sang Gu Lim,Young Soo Kim,Hyung Kyun Han,Kwang Suk Kim,Dae Jung Kim,Eon Jong Kang,Sang Gyu Son,Eung Oh Kim 한국발생생물학회 2012 발생과 생식 Vol.16 No.4
The effects of ovulation induction in ussurian bullhead, Leiocassis ussuriensis, were investigated by treating ussurian bullhead with hCG, LHRHa, GnRHa, ovaprim, and pimozide. hCG was injected to ussurian bullhead at 0.75%NaCl, 5,000, 10,000, 20,000, and 30,000 IU, respectively. The ovulation inducement rates were 100% in 20,000 and 30,000IU. Fertilization rates were 82.7% and 79.8%. Hatching rates were 59.4% and 57.2%. Ovulation time was between 16-19hr The concentrations of LHRHa injected were 0.75 NaCl, 50, 100, 200, 300, and 300 μg/kg. The ovulation inducement rates were 100% in 300 and 400 ㎍/kg. Fertilization and hatching rates were 84.9% and 68.4% at 200 ㎍/kg. The times to ovulation were between 23 hr and 34 hr. Ovaprim of 0.75% NaCl, 1.0, 1.5, 2.0, 2.5 and 3.0 ml/kg were injected to the abdominal cavity. The ovulation inducement rate was highest at 2.0 and 3.0 ml/kg to 92% and ovulation time was between 27-38 hr. LHRHa concentrations of 0.75% NaCl, 50, 100, 200, 300 and 400 μg were injected with pimozide (1,000μg). Ovulation inducement rate was 100% from 200 to 400 IU with pimozide. Ovulation time was 22-36 h. Fertilization and hatching rates were 88.9% and 70.4% in 200 ㎍/kg with pimozide.
Subsidence after Anterior Cervical Interbody Fusion Using a Zero-Profile Device
Kang Jae-Seong,Oh Sung-Han,Cho Pyung-Goo 대한말초신경학회 2019 The Nerve Vol.5 No.2
Objective Zero-profile device (Zero-P) has generally been used as a stand-alone cage in anterior cervical discectomy and fusion (ACDF). However, using Zero-P has been reported to be associated with a high subsidence rate. The present study evaluates the factors that influence the subsidence of ACDF. Methods Records of a total of 29 patients with degenerative spondylosis resulting in radiculopathy or myelopathy between C2 and C7 who underwent ACDF with Zero-P were retrospectively reviewed. The participants received 1 or 2 level ACDF with Zero-P in our clinic from December, 2011 to December, 2017. A total of 35 treatment levels were included in the analysis. Subsidence was defined when anterior segmental heights decreased by more than 2mm during the follow-up period. The patients were divided into the subsidence and non-subsidence groups. The following factors were investigated in relation to the occurrence of subsidence: age, gender, body mass index, diabetes mellitus, cage height, perioperative distraction, segmental lordosis, pain score, fusion grade, anterior/posterior segmental height ratio, and cage distance. Results Cage subsidence was observed in 15 cases (15/35, 42%). Cage distance at immediate postoperation was significantly different between the 2 groups; specifically, while the group with subsidence had 2.66±1.62mm distance, the group without subsidence had 1.24±1.13mm distance (p=0.008). Conclusion This study confirms that the more deeply titanium part is inserted at the anterior edge, the more subsidence occurs. In order to decrease the occurrence of cage subsidence, Zero-P cages should be located the closest possible to the anterior rim of vertebral body at operation.
Sang Min Kyu,Park Jie eun,Song Dae Kwon,Jeong Jun Yang,Hwang Hee Ju,Kim Hyun woo,Kim Tae Yun,Park So Young,Kang Se Won,Patnaik Bharat Bhusan,Cha Sung‐Jae,Han Yeon Soo,Lee Hee Il,Lee Yong Seok 한국곤충학회 2022 Entomological Research Vol.52 No.6
Ticks are vectors that cause disease by transmitting bacteria, viruses, and protozoa to humans or animals. The Asian longhorned tick Haemaphysalis longicornis, a vector of medical and veterinary importance, is widely distributed in the Korean peninsula and can transmit various pathogens including Rickettsia spp., Borrelia spp., Francisella spp., Coxiella spp., and severe fever with thrombocytopenia syndrome virus (SFTS virus). Despite the abundance and importance, studies on the microbiome of H. longicornis in Korea are limiting. Here we first report the microbiome diversity of H. longicornis in terms of region, stage, and sex. H. longicornis used in this study were collected from 16 different regions. The V3-V4 region was amplified and sequenced by MiSeq platform. The microbial diversity analysis was performed using Qiime2. A total of 1,754,418 non-chimeric reads were obtained from a total of 46 samples, and an average of 126 operational taxonomic units (OTUs) and a total of 1,398 OTUs were identified. Our results were used for H. longicornis microbial community database construction for each region that enables to identify singularities in each region.
Novel Hierarchical Test Architecture for SOC Test Methodology Using IEEE Test Standards
Han, Dong-Kwan,Lee, Yong,Kang, Sung-Ho The Institute of Electronics and Information Engin 2012 Journal of semiconductor technology and science Vol.12 No.3
SOC test methodology in ultra deep submicron (UDSM) technology with reasonable test time and cost has begun to satisfy high quality and reliability of the product. A novel hierarchical test architecture using IEEE standard 1149.1, 1149.7 and 1500 compliant facilities is proposed for the purpose of supporting flexible test environment to ensure SOC test methodology. Each embedded core in a system-on- a-chip (SOC) is controlled by test access ports (TAP) and TAP controller of IEEE standard 1149.1 as well as tested using IEEE standard 1500. An SOC device including TAPed cores is hierarchically organized by IEEE standard 1149.7 in wafer and chip level. As a result, it is possible to select/deselect all cores embedded in an SOC flexibly and reduce test cost dramatically using star scan topology.