생물반응기와 양액재배 시스템을 이용한 무병 씨감자의 대량생산

한은주,염미란,박현춘,Paek, Kee-Yoeup 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

씨감자를 사용할 때 발생하는 수량감소는 씨감자의 퇴화에 그 원인이 있는데 이는 PLRV, PVY와 같은 virus 감염에 의한 병리적 원인, 비정상적인 물질대사에 의해 강엽 내 성분량의 변화가 씨감자의 생산력을 저하시키는 생리적 원인 및 기상조건과 같은 환경요인이 주로 관여한다. 이에 따라 생장점 배양에 의해 무병주를 획득하고 이를 기내에서 대량으로 증식시킨 후 격리 온실에서 씨감자를 생산하는 방법이 널리 이용되고 있고 기내 괴경형성에 대한 실험도 많이 이루어지고 있다. 기내에서의 shoot 증식에는 여러 가지 생장조절물질, 특히 kinetin, BAP, zeatin등이 효과적으로 이용되며 shoot로 부터의 기내 소괴경의 유도는 배지 내 당 농도의 증가, ancimidol과 같은 생장억제제, 암 배양이나 단일조건의 부여에 의해 쉽게 이루어진다. 기내 줄기의 삽목 증식 후 이를 노지에 이식하여 소괴경을 형성시키는 방법의 경우, 현재까지는 주로 배지조건에 치중하여 왔으며 광 조건, 온도, 배양용기의 환기 등에 관한 연구는 많이 되어 있지 않은 상태이다. 따라서 노지에서의 순화율 및 이후의 생장 저하 등 많은 문제점이 발생하고 있다. 또한 한정된 크기의 배양용기를 이용함에 따라 신초의 길이 생장이 억제되고 이에 따라 마디의 수도 감소하여 증식 효율이 낮아지게 된다. 따라서 포장에서의 원활한 순화와 식물체 생율을 위해서는 배양환경, 즉 광도, 광 주기, 배양용기의 환기방안, 기내 광합성 유도 등에 대한 연구와 함께 새로운 배양용기의 개발이 필요하다. 한편, 기내 괴경을 이용한 씨감자 생산은 시간, 노동력 및 생산비용이 많이 소요되는 것에 비해 생산 효율은 높지 않은 등 여러 문제점들이 지적되고 있다 .최근 많이 연구되고 있는 양액 재배에 의한 무병 종서 생산은 기내 소 괴경 이용 방식에 비해 괴경 크기가 크고 식물체 당 괴경 수가 월등히 많아 직접 씨감자로 이용할 수 있는 장점을 가지고 있다. 본 실험에서는 생물 반응기에서의 배양 방식이 감자 shoot의 기내 생장에 미치는 영향과 양액 재배 시스템에 따른 식물체 생육 및 괴경 형성 정도를 비교하였다.

미생물을 이용한 Gellan Gum 생성의 Kinetic에 관한 연구

金春永,鄭奉佑,張光燁,李銀美 全北大學校 1992 論文集 Vol.34 No.-

Polysaccharides produced microbially, have shown themselves to be commercially significant. The reasons for the commercial exploitation of these microbial polysaccharides is because of their unique physical and consistant chemical properties and regularity of supply. Gellan gum is a recently discovered extracellular polysaccaride. This is an anionic heteropolysaccharide that forms viscous solutions and can under certain conditions, form thermoreversible gels with characteristics similar to those formed by both carrigeenan and agar. The aspect of Gellan gum produced by Pseudomonas elodea were investigated under various concentration of carbon sources and nitrogen sources. Sucrose and glucose are used as carbon source, and Bacto-peptone, Promosoy and (NH_4)_2SO_4 are used as nitrogen source. The maximum Gellan gum production was obtained in the culture medium 30.0g/L glucose, 0.6g/L Promosoy at pH 6.6, 37℃. The molecular weight of Gellan gum was 5 × 10 exp (5) which was determined by gel permeation chromatography.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

중국 운남성 소수민족무용

최미연,천은혜,김주연 세계민족무용연구소 2002 민족무용 Vol.- No.2

FORREST II 궤양 출혈의 적절한 치료 유용성 : 전향적 무작위 연구

김성은,이선영,신정은,주미순,전정현,이경은 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Objectives : This study is to compare the clinical and cost effectiveness of various pharmacologic therapies with of without endoscopic procedure in the Forrest II ulcer. Methods : Between May 2001 and June 2002, total of 58 Forrest II bleeding activity patients (37 cases of NBVV, 6 adherent blood clots, 9 flat red spot, and 6 flat black spot) with gastric ulcer(32 cases) and duodenal ulcer(26 cases) were analyzed. UGI endoscopy was performed within 12 hours of the first bleeding episodes, and underwent repetitive endoscopy after 48h. All the patients were randomly assigned to receive somatostatin(group I), PPI(omeparzole : group II), only H2 blocker (famotidine, group III), or endoscopic injection therapy followed by famotidine (group IV). We compared with rebleeding rates, changes of ulcer size, and modified estimated costs for 3 day-hospital in four groups respectively. Results : 1) Twelve patients experienced rebleeding(20.7%). 2) The rates of rebleeding were 16.6% (2/12) in group I, 28.6%(4/14) in group II, 5.9%(1/17) in group III, 26.7% in group IV. There was no significant difference in rebleeding rate among the groups, but there was low rebleeding tendency in group III, compared with group II(p=0.087).3)Type of stigmata including non-bleeding visible vessels and adherent clots were associated with an increased rate of recurrent bleeding(p=0.01).4) When modified estimated costs were calculated, group III could be treated at the lowest cost(p<0.05). Conclusion : In Forrest II bleeding ulcer, medical therapy, especially famotidine could be suggested prudently as a proper treatment modality for this lesion, considering the cost-effecti-veness. 목적: 위십이지장의 궤양성 출혈은 대부분 특별한 치료없이 보존적인 치료만으로 지혈된다. 그러나 활동성 출혈이 있는 Forrest II 궤양의 경우는 흔히 내시경적인 치료를 하게 되는데 최근 여러 연구에서 약물치료와 비교 연구가 있었으나 다양한 약물들과 내시경 치료의 효과 및 비용-효용성의 비교는 이루어지지 않았다. 방법: 2001년 5월부터 2002년 6월가지 Forrest II 궤양출혈 환자 68명을 전향적으로 포함시켰고 이중 위암으로 진단된 경우와 내시경의 추적 검사를 거부한 경우 또는 다른 중증의 내과적 질환이 동반된 경우를 제외시켜 58명의 환자를 대상으로 하였다. 대상 환자 58명은 위궤양이 32명이고 십이지장 궤양이 26명으로 비출혈성노출 혈관 37명, 신선 부착 혈괴 6명, 적색반 9명, 그리고 흑색반 6명이었다. 초기 상부 위장관 내시경은 내원 후 12시간 이내에 이루어졌고 추적 검사는 48시간 이후에 시행되었다. 치료는 네 개의 군으로 나누어 무작위로 소마토스타틴 투여군(1군), 프로톤펌프 억제제 투여군 (2군), H₂수용체 차단제 투여군(3군), 그리고 내시경적 지혈제 주사군(4군)으로 치료하였고 각 군의 재출혈 여부, 혈압, 수혈, 궤양의 크기, 급성 출혈 흔적 등을 비교 관찰하였고 입원 3일간의 입원치료비용을 계산하였다. 결과: 1) 재출혈은 12명(20.7%) 에서 발생하였다. 2) 각군에서의 재출혈은 제1군이 16.6%(2/12), 제2군에서 28.6%(4/14), 제3군이 5.9%(1/17), 제4군이 26.7%(4/15)로 각 군의 재출혈 빈도는 차이가 없었으나 제3군에서 제2군에 비해 재출혈이 낮은 경향을 보였다.(p=0.087).3) 재출혈에 영향을 미치는 인자는 노출 현환과 신선 혈괴였다(p=0.01). 4)평균 입원치료 비용은 제3군이 가장 적어 높은 치료 비용-효과를 보였다.(p<0.05).

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.

The Korean Academy of Tuberculosis and Respiratory Diseases : Slide Session ; OS-063 : Lung Cancer ; Aspirin, NSAID, and Statins and Risk of Lung Cancer: A Gene by Drug Interaction Analysis

( Eun Mi Chun ),( Mi Sun Lee ),( Kofi Asomaning ),( Rebecca Heist ),( Lii Su ),( David C Christiani ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Epidemiologic studies have been shown that the use of chemopreventive drugs may decrease cancer risk. Therefore, we aimed to investigate the effects of aspirin, NSAIDs, and statins use for reduced risk of lung cancer. We further examined whether genetic polymorphisms modify these effects. Methods: The study population consisted of 1,582 lung cancer patients and 816 controls from the Massachusetts General Hospital. SNPs were assessed in genes involved in phase II metabolism (GSTs, NAT2, EPHX, and NQO1), DNA repair (ERCC1, ERCC2, and XRCC1), DNA methylation (MTHFR C677T and A1298C), and angiogenesis (VEGF +936C>T, -460T>C, and +405G>C) pathways. Results: We used logistic regression to estimate the odds ratios (OR),and after adjustmentfor covariates, the use of statins and aspirin were signifi cantly associated with reduced risk of lung cancer [OR 0.74, 95% confi dence interval (CI) 0.56 to 0.97 for statins and OR 0.36, 95% CI 0.27 to 0.47 for aspirin, respectively], whereas an inverse association was seen with NSAIDs. Signifi cant modifying effects were found by NAD( P)H quinine oxidoreductase (NQO1) gene polymorphisms with the use of statins (OR 0.57, 95% CI 0.40 to 0.80 for CC versus OR 1.21, 95% CI 0.74 to 1.99 for CT or TT, P = 0.03) and by glutathione S-transferases (GSTP1) with aspirin use (OR 0.23, 95% CI 0.15 to 0.35 for AA versus OR 0.46, 95% CI 0.32 to 0.67 for AG or GG, P = 0.04).Conclusions: Our results indicate that statins and aspirin use may attenuate the risk of lung cancer and genetic polymorphism may modify these associations.

Identification of eleven species of the Pleuronectidae family using DNA-based techniques

Eun-Mi Kim,Mi Nan Lee,Chun-Mae Dong,Eun Soo Noh,Young-Ok Kim 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.11

Flatfish are one of the largest families in the order Pleuronectiformes and are economically important edible marine fish species. However, they have similar morphological characteristics leading to challenges in classifying correctly, which may result in mislabeling and illegal sales, such as fraudulent labeling of processed food. Therefore, accurate identification is important to ensure the quality and safety of domestic markets in Korea. Species-specific primers were prepared from the mainly consumed eleven species of the order Pleuronectiformes. To rapidly identify the 11 flatfish species, a highly efficient, rapid, multiplex polymerase chain reaction (PCR) with species-specific primers was developed. Species-specific primer sets were designed for the mitochondrial DNA cytochrome c oxidase subunit I gene. Species-specific multiplex PCR (MSS-PCR) either specifically amplified a PCR product of a unique size or failed. This MSS-PCR analysis is easy to perform and yields reliable results in less time than the previous Sanger sequencing methods. This technique could be a powerful tool for the identification of the 11 species b the family Pleuronectidae and can contribute to the prevention of falsified labeling and protection of consumer rights.

Smoking Cessation Strategies Targeting Specific Populations

Chun, Eun Mi The Korean Academy of Tuberculosis and Respiratory 2019 Tuberculosis and Respiratory Diseases Vol.82 No.1

Quitting smoking helps smokers maintain their health and extend their lifespan by 10 or more years. Treatment strategies for smoking cessation should be tailored to individual smokers with special needs based on their specific circumstances. It is recommended that pregnant women adopt smoking cessation through counseling and behavioral interventions because the safety of medications has yet to be established. Counseling is the main strategy for smoking cessation in adolescents and nicotine replacement therapy can be used with caution in individuals with serious nicotine dependence. It is important for smokers with psychiatric diseases to quit smoking following accurate assessment of their depression status. Nicotine replacement therapy, varenicline, and bupropion can be used for smoking cessation in smokers with psychiatric disorders. The incidence of cardiovascular disease decreased according to the smoking status and the duration of smoking cessation. In smokers with chronic obstructive pulmonary disease (COPD) who used a combination of counseling and pharmacotherapy the quitting rate was more than twice as high as subjects who used behavioral interventions alone. Varenicline can be used as the most effective anti-smoking drug by most smokers including those with psychiatric disorders, cardiovascular disease, and COPD.