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사람-사람 하이브리도마의 생산을 위한 fusion partner cell의 개발
안지영,김혜원,서동상 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1
This study was performed to establish human hybridmyeloma cell lines as fusion partner cells for producing human-human hybridoma. Human myeloma, SKO-007(J3), and human cervix epitheloid carcinoma cell line, HeLa MR, were fused by using with 50% PEG, and cultured in RPMI 1640 medium containing 15% FBS. Hybrids of SKO-007(J3) and HeLa MR were selected in RPMI 1640 media containing 6-thioguanine(6-TG ; gradually increasing concentration from 10 ㎍/ml to 20 ㎍/ml, and then gradually decreasing to 10 ㎍/ml) for 6 weeks. 6-TG resistant cells were collected and cloned by limiting dilution method. The fused myelomas with HeLa MR grew more rapidly than the fused cells with a small number of HeLa MR. Their doubling time was 72 hours.
Superficial intense focused ultrasound on periorbital wrinkle
( Dong Hye Suh ),( Hyung-jin Park ),( Sang Jun Lee ),( Kye Young Song ),( Min Kyung Shin ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2
Background: The periorbital wrinkles are easily perceived evidence of aging, so become a major concern for many patients. Various treatments have been attempted to improve periorbital wrinkles, but the need for new treatments that are less invasive and more effective is still high. Objectives: In this study, we evaluated the safety, clinical and histological effects of intense focused ultrasound (IFUS) using only a 1.5 mm transducer in the management of periorbital wrinkles. Methods: Ten adult Korean females were enrolled. The treatment effect and safety profile were evaluated up to three months after one session of IFUS treatment on the periorbital wrinkles. Results: The mean subjective satisfaction score was 3.2±0.79 (mean±standard deviation) by five- point scale. The mean objective clinical improvement score was highest in the fine wrinkle on the crow's feet area and lowest in the deep wrinkles of the infraorbital and crow’s feet area. Histometrically, increase of collagen and elastic fiber density was observed in the all layers of dermis. No serious side effects occurred after the treatment. Conclusion: Intense focused ultrasound treatment using a 1.5-mm transducer alone can significantly improve periocular wrinkles after a single treatment with a good safety profile.
P111 The efficacy and safety of fractional unipolar radiofrequency on facial tightening
( Dong-hye Suh ),( Eun Jung Byun ),( Sang-jun Lee ),( Kye Yong Song ),( Hei Sung Kim ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2
<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: The medical use of radiofrequency (RF) is based on an oscillating electrical current that forces collisions between charged molecules and ions, which are then transformed into heat. Water is the main target for this process. RF-generated tissue heating has different biological and clinical effects, depending on the depth of tissue targeted, the frequency used, and the specific cooling of the dermis and epidermis. RF can be delivered using monopolar, bipolar, and unipolar devices. With unipolar RF, there is one electrode, no grounding pad, and a large field of RF is emitted in an omnidirectional field around a single electrode. A unipolar hand-piece delivers RF energy to an estimated depth of up to 20 mm. Objectives: Herein, we aimed to assess the efficacy and safety of a fractional unipolar RF device on facial tightening. Methods: Fractional unipolar RF (tuneface hand-piece, AccentTM Prime, Alma Lasers, Inc., Fort Lauderdale, FL) was applied to the individual’s face every 2 weeks for a total of 5 sessions. Results: A total of 14 patients (all female, mean age: 49.7 years) were included in this study. The majority of patients (85.7%) answered that the results were good or excellent. Consensus ratings by 2 physicians found that the objective outcomes were divided among “75-100% improvement (35.7%)”, “50-74% improvement (50%)”, “25-49% improvement (14.3%)”, and “improvement of less than 25% (0%)”. Skin biopsies from the face revealed increase of collagen in the dermis. The incidence of complications was low and minor. Conclusion: Unipolar RF device with a novel mobile energy delivery offers an additional step in non-ablative laser resurfacing for rhytides and skin laxity.
( Dong Hye Suh ),( Hee Kyung Lim ),( Hyun Joo Kim ),( Sang Jun Lee ),( Tae In Kim ),( Ki Heon Jeong ),( Min Kyung Shin ),( Kye Yong Song ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Various treatment modalities have been used to treat acne scars. Carbon dioxide (CO2) gas subcision into the dermis by needle with high pressure can cause breakage of fibrotic collagen taking effect of not only subcision but also CO2 gas effects. CO2 fractional laser is the effective and commonly used tool for acne scar to ablation. Objectives: This study was conducted to evaluate the efficacy and safety of subcision with CO2 gas and CO2 fractional laser. Methods: Fourteen patients with skin phototype III and V with acne scars were treated 3 sessions of CO2 gas subcision with 2-week interval and 2 sessions of fractional laser with 4-week intervals. The clinical improvements were assessed in terms of acne scar using a quartile grading scale. Any adverse effects were assessed during the entire study period. A punch biopsy was performed before and after treatment in 10 patients for histologic analysis. Results: Excellent result show 7%, marked result in 57%, moderate result in 29%, and mild improved show in 7%. Histologic evaluation of skin biopsy samples using hematoxylin and eosin and Victoria blue stains showed greater dermal collagen with thickening of the dermis and straightening of elastic fibers in the reticular dermis after treatment. Conclusion: Combination therapy using subcision CO2 gas and fractional laser with 2 sessions was satisfactory and safe for acne scar.
( Dong-hye Suh ),( Haneul Oh ),( Sang Jun Lee ),( Hyun Joo Kim ),( Hwa Jung Ryu ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Acne vulgaris is one of the most common dermatological diseases, and it is known that various factors are related to triggering this condition in a patient. In particular, although the use of cosmetics is thought to be associated with the onset of a patient developing acne, there are few studies that have been conducted to date on this disease and its related factors. Objectives: To investigate the relationship between cosmetic use patterns and acne, in addition to clinical features for acne patients. Methods: To begin with, a total of 539 patients with acne vulgaris, who visited three dermatologic clinics in Korea, were asked to complete a questionnaire regarding their condition in relation to clinical features and exacerbating factors, including the use of cosmetics. Results: Among the respondents, 38.1% of patients who failed treatment answered that the condition of their acne was worsened by the continued use of cosmetics, which was significantly higher than 26.3% of the surveyed treatment-naive patients (p<0.05). In this relation, double cleansing and the number of cosmetics in use were also noted in higher numbers than in the treatment-failure patients. As noted, the liquid-type foundation and concealer were frequently used in this group. Conclusion: This study showed that overall dermatologists should consider advising about cosmetic use to acne patients along with medical treatment options for the patients with acne.
P138 : Outcomes of polydioxanone thread lifting for facial rejuvenation
( Dong Hye Suh ),( Hee Won Jang ),( Sang Jun Lee ),( Won Seok Lee ),( Hwa Jung Ryu ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Thread lifting is a minimally invasive technique for facial rejuvenation. Various devices for thread lifting using polydioxanone are popular in aesthetic clinics in Korea, but there have been a few studies regarding its use. Objectives: To describe polydioxanone thread and techniques adopted to counteract the descent and laxity of the face. Methods: A retrospective chart review was conducted over a 24-month period. A total of 31 thread lifting procedures were performed. On each side, five bidirectional cog threads were used in the procedure for the flabby skin of the nasolabial folds. And the procedure was done on the marionette line using two twin threads. Results: In most patients (87%), the results obtained were considered satisfactory. Consensus ratings by two physicians found that objective outcomes were divided among “excellent,”“good,”“fair” and “poor”. Texture-wise, the outcome ratings were 13 as excellent and 9 as good. Lifting-wise, ratings were 11 as excellent and 6 as good. The incidence of complications was low and not serious. Conclusion: Facial rejuvenation using polydioxanone thread isa safe and effective procedure associated with only minor complications when performed on patients with modest face sagging, fine wrinkles, and marked facial pores.