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( Chang Su Kim ),( Jung Bok Lee ),( Beam Soo Kim ),( Young Ho Nam ),( Kee Sun Shin ),( Jin Won Kim ),( Jang Eok Kim ),( Gi Seok Kwon ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
The possibility of using hyphomycete fungi as suitable biocontrol agents against greenhouse whitefly has led to the isolation of various insect pathogenic fungi. Among them is Beauveria bassiana, one of the most studied entomopathogenic fungi. The objective of this study was to use B. bassiana M130 as an insecticidal agent against the greenhouse whitefly. M130 isolated from infected insects is known to be a biocontrol agent against greenhouse whitefly. Phylogenetic classification of M130 was determined according to its morphological features and 18S rRNA sequence analysis. M130 was identified as B. bassiana M130 and showed chitinase (342.28 units/ml) and protease (461.70 units/ml) activities, which were involved in the invasion of the host through the outer cuticle layer, thus killing them. The insecticidal activity was 55.2% in petri-dish test, 84.6% in pot test, and 45.3% in field test. The results of this study indicate that B. bassiana has potential as a biological agent for the control of greenhouse whitefly to replace chemical pesticides.
미강추출물을 이용한 곤충병원성 곰팡이 Beauveria bassiana의 최적 배양조건 및 효소활성
김창수(Chang-Su Kim),이중복(Jung-Bok Lee),김범수(Beam-Soo Kim),이민혜(Min-Hye Lee),강경묵(Kyeong-Muk Kang),주우홍(Woo-Hong Joo),김진원(Jin-Won Kim),임대준(Dae-Joon Im),권기석(Gi-Seok Kwon) 한국생명과학회 2013 생명과학회지 Vol.23 No.8
본 연구에서는 온실가루이 방제를 위하여 감염된 이병체로부터 균주 분리 및 농촌진흥청 및 경상북도농업기술원에서 균주를 분양 받아 5 종의 균주를 확보하여, 최종적으로 활성이 가장 높은 분리균주 M130을 선발하였다. Beauveria bassiana M130을 이용하여 균주가 갖는 효소학적 활성을 분석하였다. Chitinase와 protease의 활성을 조사한 결과, 타 균주에 비하여 높은 효소학적 활성을 가지는 조건을 얻었으며, 선발된 균주의 포자생산의 최적화를 위한 방안으로 PDB (potato dextrose broth)배지와 미강추출물을 이용한 ERBM (extracted rice bran medium)을 비교하여, 더 높은 생육도와 포자 생성량을 보인 ERBM을 본 균주를 배양하는 기본배지로 선발하였다. ERBM을 이용한 액체배양시의 최적조건으로, 기본배지인 ERBM에 0.5% (NH₄)₂SO₄를 첨가하여 pH 5, 28℃에서 배양최적화를 하였다. 향후 곤충병원성곰팡이 Beauveria bassiana M130의 최적화 조건에 따라 배양을 한다면 포자의 생산량 및 방제효과가 높아질 것으로 사료된다. The greenhouse whitefly, Bemisia tabaci, is considered one of the most destructive pests of crops. In this study, we aimed to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana M130 using rice bran. The optimal initial pH for the spore production of B. bassiana using extracted rice bran medium was 5.2 and 28℃. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 0.5% (NH₄)₂SO₄, with extracted rice bran 8:1. Using this medium, a production level of 2.15×10<SUP>9</SUP> spores per ml was obtained after six days from culture inoculation at 28℃ in a rotary shaking incubator at 130 rpm. In addition, the specific activities of extracellular enzymes of chitinase and protease were 4,296μmol and 375 μmol, respectively. These results suggest that Beauveria bassiana M130 could be a biocontroller for the greenhouse whitefly.
이낙주,김범수,Kim, Beam Soo 대한기계학회 1977 대한기계학회논문집 Vol.1 No.3
In this paper, approximate solutions of the von Karman equations for the free flexural vibration of a transversely isotropic thin rectangular plate with two simply supported edges and two clamped edges are obtained. Applying one term Ritz-Galerkin procedure, the spatial dependent part of the equation is separated and time dependent function is found to be the Duffing's equation. Then the relation between nonlinear period and amplitude of the vibration is obtained by using averaging method which is a method of the perturbation procedure. It can be seen that averaging method is easy and agrees well with prior results.
( Jong Ok Jang ),( Jung Bok Lee ),( Beam Soo Kim ),( Sun Chul Kang ),( Cher Won Hwang ),( Kee Sun Shin ),( Gi Seok Kwon ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.3
BACKGROUND: Chemical fungicides not only may pollute the ecosystem but also can be environmentally hazardous, as the chemicals accumulate in soil. Biological control is a frequently-used environment-friendly alternative to chemical pesticides in phytopathogen management. However, the use of microbial products as fungicides has limitations. This study isolated and characterized a three-antifungal-enzyme (chitinase,cellulase,andβ-1,3-glucanase)-producing bacterium, and examined the conditions required to optimize the production of the antifungal enzymes. METHOD AND RESULTS: The antifungal enzymes chitinase, cellulase, and β-1,3-glucanase were produced by bacteria isolated from an sawmill in Korea. Based on the 16S ribosomal DNA sequence analysis, the bacterial strain AM50 was identical to Streptomyces sp. And their antifungal activity was optimized when Streptomyces sp. AM50 was grown aerobically in a medium composed of 0.4% chitin, 0.4% starch, 0.2% ammonium sulfate, 0.11% Na2HPO4, 0.07% KH2PO4, 0.0001% MgSO4, and 0.0001% MnSO4 at 30℃. A culture broth of Streptomyces sp. AM50 showed antifungal activity towards the hyphae of plant pathogenic fungi, including hyphae swelling and lysis in P. capsici, factors that may contribute to its suppression of plant pathogenic fungi. CONCLUSION(S): This study demonstrated the multi- antifungal enzyme production by Streptomyces sp. AM50 for the biological control of major plant pathogens. Further studies will investigate the synergistic effect, to the growth regulations by biogenic amines and antifungal enzyme gene promoter.
Jang, Jong-Ok,Lee, Jung-Bok,Kim, Beam-Soo,Kang, Sun-Chul,Hwang, Cher-Won,Shin, Kee-Sun,Kwon, Gi-Seok The Korean Society of Environmental Agriculture 2011 한국환경농학회지 Vol.30 No.3
BACKGROUND: Chemical fungicides not only may pollute the ecosystem but also can be environmentally hazardous, as the chemicals accumulate in soil. Biological control is a frequently-used environment-friendly alternative to chemical pesticides in phytopathogen management. However, the use of microbial products as fungicides has limitations. This study isolated and characterized a three-antifungal-enzyme (chitinase, cellulase, and ${\beta}$-1,3-glucanase)-producing bacterium, and examined the conditions required to optimize the production of the antifungal enzymes. METHOD AND RESULTS: The antifungal enzymes chitinase, cellulase, and ${\beta}$-1,3-glucanase were produced by bacteria isolated from an sawmill in Korea. Based on the 16S ribosomal DNA sequence analysis, the bacterial strain AM50 was identical to Streptomyces sp. And their antifungal activity was optimized when Streptomyces sp. AM50 was grown aerobically in a medium composed of 0.4% chitin, 0.4% starch, 0.2% ammonium sulfate, 0.11% $Na_2HPO_4$, 0.07% $KH_2PO_4$, 0.0001% $MgSO_4$, and 0.0001% $MnSO_4$ at $30^{\circ}C$. A culture broth of Streptomyces sp. AM50 showed antifungal activity towards the hyphae of plant pathogenic fungi, including hyphae swelling and lysis in P. capsici, factors that may contribute to its suppression of plant pathogenic fungi. CONCLUSION(S): This study demonstrated the multiantifungal enzyme production by Streptomyces sp. AM50 for the biological control of major plant pathogens. Further studies will investigate the synergistic effect, to the growth regulations by biogenic amines and antifungal enzyme gene promoter.
조범규,최한주,장용수,신태용,이강현,황성오,김현,Cho, Beam-Gyu,Choi, Han-Joo,Jang, Yang-Soo,Shin, Tae-Yang,Lee, Kang-Hyun,Hwang, Sung-Oh,Kim, Hyun 대한임상독성학회 2007 대한임상독성학회지 Vol.5 No.1
Cyanide has been well known from antiquity. Cyanide poisoning causes histotoxic hypoxia--inhibition of mitochondrial cytochrome oxidase with disruption of the ability of cells to use oxygen. The mortality rate is 95% within 30 minutes. Acute cyanide poisoning is mostly the result of attempted suicide, and because of the high doses typically employed it usually is lethal within minutes. Also acute cyanide poisoning leads to a very poor prognosis because medical personnel can't detect cyanide intoxication and so many hospital haven't antidote. We report two cases of cyanide poisoning which responded well to treatment with the Taylor cyanide antidote package.