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Production of methionine γ-Lyase in recombinant Citrobacter freundii bearing the hemoglobin gene
( Huseyin Kahraman ),( Emel Aytan ),( Asli Giray Kurt ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9
The production of antileukemic enzyme methionine γ-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine γ-liyase production. Methionine γ-liyase production by Citrobacter freundii and its vgb? and vgb+ bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30oC in a time-course manner. The vgb+ and especially the carbon type had a dramatic effect on methionine γ-liyase production. The vgb+ strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb? strain, respectively. [BMB reports 2011; 44(9): 590-594]