산형과 식물에서의 falcarinol(n - heptadeca - 1,9 - dien - 4,6 - diyn - 3 - ol) 의 검색

이규승,손현주,장기철,나효환 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2

The occurrence of falcarinol(n-heptadeca-1, 9-dien-4,6-diyn-3-o1)in the umbelliferous plants such as Ledebouriello seseloides WOLFF., Cnidium officinale MAKINO, Foeniculmn vulgare GAERTNER, Torilis japonica(HOLTT.) DC. Bupleurum falcatum L.,Angelica gigas NAKAI, Oenanthe javanica (BL.) DC. and Daucus carota var. satin DC. was investigated using gas chromatography/mass spectrometry(GC/MS). Falcarinol was found in the root of Ledebouriella seseloides, the stem and the root of Oenanthe javanica, and the root of Daucus carota var. sativa in which its contents were 1,055 ㎍/g, 289 ㎍/g , 179 ㎍/g , and 212 ㎍/g , respectively, while it was not found in the other plants.

수생식물(水生植物)을 이용(利用)한 수질오염원제거(水質汚染源除去)에 관(關)한 연구(硏究) - 제2보(第2報) 부레옥잠의 영양염류(營養鹽類) 및 중금속(重金屬) 제거효과(除去效果)

이규승,김문규,변종영,이종식,Lee, Kyu-Seung,Kim, Moon-Kyu,Pyon, Jong-Yeong,Lee, Jong-Sik 한국잡초학회 1985 Weed&Turfgrass Science Vol.5 No.2

열대성(熱帶性) 수생식물(水生植物)인 부레옥잠(Eichhornia crassipes)의 오염물질(汚染物質) 제거효율(除去效率)에 관한 실험(實驗)을 통해 나타난 결과(結果)를 요약(要約)하면 다음과 같다. 1. 초기농도(初期濃度) 10, 25 및 50 ppm 용액(溶液)에 대해 처리(處理) 3일후(日後) 질산성질소(窒酸性窒素)는 0.7, 0.9 및 1.2ppm 그리고 인산염(燐酸鹽)은 0.1, 0.2 및 0.5ppm 으로 제거(除去)되었으며, 이러한 영양염류(營養鹽類)의 제거능력(除去能力)은 초기(初期)에서 더욱 높았다. 2. 중금속(重金屬)의 제거(除去)는 Cu, Pb에서 컸으며, 중금속(重金屬)에 의한 부레옥잠 피해는 Cu, C에서 심하였고, 식물체에 의한 중금속(重金屬) 제거경향(除去傾向)은 대상 중금속의 종류(種類)에 따라 서로 달랐다. 3. 식물체(植物體)로 흡수(吸收) 제거(除去)된 중금속량(重金屬量)은 농도(濃度)가 높을수록 많았고, 중금속별(重金屬別)로는 Cu, Pb, Cr, Cd의 순이었으며, 식물체내(植物體內)에서의 분포는 뿌리 부분에 많았다. 4. 부레옥잠 처리(處理) 10일후(日後)의 식물체내(植物體內)로의 중금속(重金屬) 흡수(吸收)와 뿌리에 흡착(吸着)은 모두 Cu와 Pb는 Cr과 Cd에 비하여 컸으며, 뿌리에의 흡착량(吸着量)은 농도(濃度)가 높을수록 많았고, 중금속별(重金屬別)로는 Cu, Pb, Cd, Cr 순(順)이었다. Removal of water pollutants by water hyacinth was examined with two nutrients, $NO_3$-N, $PO_4$-P and four heavy metals, Cu, Pb, Cd, Cr under laboratory conditions. $NO_3$-N was reduced to 0.7, 0.9 and 1.2 ppm, and 0.1, 0.2 and 0.5 ppm in $NO_4$-P from 10, 25 and 50 ppm 3 days after treatment, respectively. Among heavy metals Cu and Pb were removed faster and higher than Cd and Cr and also amount of heavy metals absorbed by water hyacinth was higher in the order of Cu > Pb > Cr > Cd. Distribution of heavy metals in this plant was higher in roots than in leaves and amount absorbed in roots was related to the treated concentrations. The harmful effect on growth of water hyacinth was observed in Cu and Cd.

On The Extraction Methods for the Residues of Carbofuran and its two Metabolites from Fortified Soil

李奎承 제주대학교 1976 논문집 Vol.8 No.-

現在 우리나라에서 水稻의 멸구類, 매미충類의 防除劑로 使用되고 있는 carbofuran (商品名: 후라단, 큐라텔)과 이의 두가지 중요한 代謝物인 3-keto carbofuran과 3-hydroxy carbofuran의 土壤中 殘留成分의 分析에 관해 서로 다른 3種의 抽出方法으로 比較 檢討하였다. 즉 酸에 의한 加水分解法 (FMC 法)과 水分含量에 따른 acetonitrile을 利用한 blending法 및 混合溶媒를 利用한 blending法 (methylene chloride/methanol: 5/2)으로 토양시료에 첨가하여 준 各 藥劑의 殘留分을 抽出하여 1-fluoro-2,4-dinitrobenzene으로 誘導體를 만들어 ECD를 부착한 gas chromatography法에 의해 수행하였다. 實驗結果를 要約하면 다음과 같다. 1. 各 약제성분의 誘導體는 gas chromatogram 上에서 完全히 分離되었으며, 각각의 檢出最紙限界(LDA)는 carbofuran은 0.08 10-¹²g/sec, 그리고 3-hydroxy carbofuran은 0.28 10-¹²g/sec이었다. 2. 酸에 의한 加水分解法에서는 carbofuran 59.6%, 3-keto carbofuran 40.0% 그리고 3-hydroxy carbofuran 51.4%의 回收率을 보였고, 3. 土壤試料의 水分含量을 달리하여 acetonitrile을 抽出溶媒로해서 blending한 方法은 carbofuran의 回收率이 70% 以上이었으며, 특히 水分含量이 40∼60%에서는 72%以上의 回收率을 나타냈다. 4. methylene chloride와 methanol의 混合溶媒로 blending한 方法은 carbofuran의 경우 53.4%, 3-keto carbofuran은 42.0% 이었으며, 특히 3-hydroxy carbofuran의 回收率은 11.4%로 아주 낮았다. 5. 混合溶媒로 抽出한 試料를 지금까지 使用하였던 3% OV-17 column 대신 5% OV-7 column을 利用하여 比較한 결과, carbofuran은 66.0%로 또 3-keto carbofuran은 63.0%로 현저히 높은 수준은 보였으나 3-hydroxy carbofuran은 오히려 8.9%로 더 낮은 水準을 보였다. 따라서 column 充塡物에 따른 藥劑別 감도에 關한 硏究가 수행어야 할 것으로 생각된다. A GLC procedure is described for the residual analysis of carbofuran and its two metabolites, 3-hydroxy carbofuran and 3-keto carbofuran, from fortified soil samples with three different extracting methed‥‥acid-hydrolysing, blending with acetonitrile/water and with mixed solvent system (methylene chloride/methanol : 5/2). All extracting samples were derivatized with 1-fluoro-2, 4-dinitrobenzene and determind with electron capture dector. Summaries are as follows: 1. Gas chromatogram of those three chemicals showed fine resolution with high sensitivity. 2. The acid-hydrolysis method was recovered 59.6% for carbofuran, 40.0% for 3-keto carbofuran and 51.4% for 3-hydroxy carbofuran. 3. Blending method with acetonitrile in different water contents of soil samples was appeared higher recoveries(up to 70%) than the other two methods. 4. Blending methed with mixed solvent system (methylene chloride/methanol: 5/2) showed the lowest recoveries among three extracting methods‥‥53.4% for carbofuran, 42.0% for 3-keto carbofuran and 11.4% for 3-hydroxy carbofuran. 5. Comparison of coumn efficiency of 3% OV-17 and 5% OV-7 was indicated much different values for the recoveries of the same sample.

자성가토의 갑상선처리가 비육효과에 미치는 영향

이규승,조충호,장경진 한국축산학회 1969 한국축산학회지 Vol.11 No.1

This study was carried out in order to determine the effects of thyroid function upon fattening of the female rabbits. Seventy five female rabbits with an average weight of 2 ㎏ were devided into iodocasein treatment group, tapazol treatment group and control group. Iodocasein treatment group was fed continuously a control ration containing 0.084 g of iodcasein per Kg body weight, and tapazol treatment group 0.25 ㎎ of tapazol. Changes of body weight were observed at an interval of 2 weeks. lifter the pariods of 1, 3, 4, 6 and 8 weeks, the rabbits were sacrificed and then lean meat yield, lean meat percentage and the weight of viscera fat were measured. The results obtained were as follows; 1. Body weight of iodocasein treatment group showed a gradual tendency of decrease by the high significance(P$lt;0.01.) in process of treatment time. Tapazol treatment group showed a trend of a little increase in process of time, reaching to 2262.6±73.39 g, the highest value at the end of experimental periods, but a significant difference was not recognized. 2. Lean meat yield sowed the significance with P$lt;0.05 after 2 weeks and there's no difference among the neighbouring groups. From 4 weeks after the treatment, in iodocasein treatment group it revealed gradually the decreasing significance with P$lt;0.01 and in the tapazol treatment group the increasing significance (P$lt;0.01), therefore it is recognized that the hypothyroid had an effect on the fattening. 3. Lean meat percentage showed the significance with P$lt;0.05 at each observation time from 4 weeks after the treatment, showing a trend of a decrease in iodocasein treatment group and a increase in tapazol treatment group. 4. Weight of viscera fat showed the marked significance with P$lt;0.01 at each observation time from weeks after the treatment. In iodocasein group it was decreased gradually and 25.0±5.22 g at the end of the experimental period, while tapazol treatment group increased gradually and reached 68.8±6.61 g at the same time.

忠南地域 마늘中 有機燐系 農藥殘留水準

崔鐘佑,李奎承 忠南大學校 環境問題硏究所 1986 環境硏究 Vol.4 No.2

Residue levels of organophosphorous pesticides in/on garlic produced 6 area in Chungnam during late May-early June, 1983 were investigated. Residues of two insecticides were ranged ND-0.35ppm for Dasuzin, ND-trace for Chlopho, and IB, fungicide, ranged ND-0.44ppm. Detection frequency was 47.7% in Dasuzin and 22.4% in IB.

忠南地域 沓土壤中 有機燐系 農樂의 殘留水準

崔種佑,李重吉,李奎承 忠南大學校 環境問題硏究所 1987 環境硏究 Vol.5 No.2

1986년 3월 20일∼4월 6일에 忠南의 大德, 公州, 錦山, 論山, 및 燕岐郡 등 5個 地域에서 總 108點의 畓土壤 試料를 採取하여 8種의 有機燐系 農藥을 대상으로 殘留水準을 조사하여 얻은 중요한 결과를 要約하면 아래와 같다. 1. 모두 7種의 農藥成分을 檢出하였으며 檢出頻度는 Dursban(38.8%), IBP(16.7%) 및 Diazinon(11.1%)의 순이었고, 平均殘留은 Dursban이 0.01ppm, IBP가 0.002ppm이었으며, 기타의 약제는 모두 흔적수준이었다. 2. 檢出試料의 殘留範圍와 平均殘留量은 Diazinon이 흔적∼0.003ppm과 흔적수준, IBP가 흔적∼0.196ppm과 0.01ppm, 그리고 Duraban은 흔적∼0.15ppm과 0.025ppm이었다. 3. 지역별도의 錦山郡과 燕岐郡에서 타지역보다 Dursban의 檢出頻度가 높은 것으로 나타났다. Residue levels of 8 organophosphorous pesticides were evaluated on 108 paddy field soils collected from 5 districts of Chungnam area during March, 20-April, 6, 1986. 7 chemicals were detected and showed the highest detection frequency on Dursban (39.8%) and the lowest on Phentoate (1.9%), also average residue levels were 0.01ppm on Dursban, 0.02ppm on IBP, and trace on the other detected pesticides.

농작업 기계 분야 : 트랙터 부착용 로더의 기구학적 시뮬레이션 프로그램 개발

이규승,김준호,노광모,박원엽 ( K . S . Lee,J . H . Kim,K . M . Noh,W . Y . Park ) 한국농업기계학회 1996 한국농업기계학회 학술발표논문집 Vol.2 No.-

환경기술 혁신전략

이규승 한국기술혁신학회 1998 기술혁신학회지 Vol.1 No.1

인사말

이규승 한국농업기계학회 2012 한국농업기계학회 학술발표논문집 Vol.17 No.1

돼지 간중의 Monooxygenase 가 Diazinon 의 분해에 미치는 영향

이규승,류종국 한국환경농학회 1990 한국환경농학회지 Vol.9 No.2

Two fractions(microsomal and soluble) were prepared by ultracentrifugation(105,000G for 1hr at 4℃) from pig liver in order to find the major factor in Diazinon degradation. The two enzyme activities showed the same value, but Diazinon was degraded three times in microsomal fraction more than in soluble fraction. And with addition of EPN, Beam and PBO, degradation of diazinon was inhibited(29, 30 and 60%) as well as Monooxygenase activity (14, 15 and 35%) in microsomal fraction, respectively. 돼지간을 두 fraction(105,000×g pellet superatant)으로 나누어 Diazinon의 분해에 관여하는 두 효소(Monooxygenase와 esterase)의 활성 및 저해제(EPN, Beam, PBO)첨가에 따른 Diazinon의 분해억제 능력을 비교 검토하였다. 1. 돼지간의 microsomal fraction과 soluble fraction에서 M. O.와 β-esterase 활성은 20분과 60분에 최고활성을 나타내었다. 2. Microsomal fraction에서의 M. O.활성은 NADPH generating system의 첨가로 1.6배의 활성증가와 지속성이 확인되었다. 3. Microsomal fraction에서의 Diazinon분해량은 30분에 최고량(0.060nM/㎎/min)을 보였으나, soluble fraction에서는 60분에 최고량(0.018nM/㎎/min)을 보여 1/3수준이었다. 4. M. O.활성에 미치는 Diazinon에 대한 EPN, Beam, PBO의 첨가효과는 Beam(14%)< EPN(15%) < PBO(35%) 순이었다. 5. Microsomal fraction에서의 Diazinon분해는 Beam(29%) < EPN(30%) < PBO(60%) 순으로 억제되었다.