http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이상수,Lee, Sang-Soo 배재대학교 자연과학연구소 1997 自然科學論文集 Vol.9 No.1
PCR 방법을 이용하여 K11 RNA 중합효소를 coding하는 Klebsiella phage gene 1을 cloning 하였고 lac 전사촉진제 조절 하에 발현시켰다. K11 RNA 중합효소는 DAEA-sephacel과 Affigel blue column chromatographies를 사용하는 상용 방법으로 분리하였다. DAEA-sephacel의 0.2-0.3 M $NH_4Cl$ 분획에서 K11 RNA 중합효소의 활성을 보였고, 다음 단계의 Affigel blue column에서 SDS-polyacryl amide gel 상의 단일 band로 분리되었다. K11 RNA 중합효소는 T7 그룹 phage RNA 중합효소로 다른 T7 그룹phage RNA 중합효소와 많은 상동성을 보인다. (대장균 phage T7, T3과 Salmonella tyhimurium phage SP6 RNA 중합효소). 이미 우리는 T7과 SP6 전사촉진제 변이체를 제조한 바 있고 T7과 SP6 RNA 중합효소의 전사촉진제 특이성을 연구한 바 있다 (이상수와 강창원, 1993). K11 RNA 중합효소의 전사촉진제 특이성을 알아보기 위해 SP6 전사촉진제 변이체를 사용하여 in vitro K11 RNA 중합효소의 활성을 측정하였다. 이 변이체 중 K11 전사촉진제와 가장 유사한 것이 가장 높은 K11 RNA 중합효소 활성을 보였다. Using the PCR(polymerase chain reaction method), gone 1 of phage K11 coding for K11 phage RNA polymerase has been cloned and expressed under the control of lac promoter. K11 phage RNA polymerase was conventionally purified through the DEAE-sephacel and Affigel blue column chromatographies. The 0.2-0.3 M $NH_4Cl$ fractions of DAEA-sephacel column chromatography showed K11 phage RNA polymerase activity and further purification with Affigel blue column chromatography showed nearly single protein band on SDS-polyacryl amide gel. K11 phage RNA polymerase, which is one of the T7 group phage RNA polymerase (E. coil phage T7, T3 and Salmonella tyhimurium phage SP6 RNA polymerase), shares high degrees of homology with the other T7 group phage RNA polymerase. Previously we constructed T7 and SP6 promoter variants and revealed promoter specificity of T7 and SP6 RNA polymerase (Lee and Kang, 1993). To investigate the promoter specificity of K11 RNA polymerase in vitro K11 promoter activity was measured with SP6 promoter variants. The SP6 promoter variant share highest degrees of sequence homology with K11 promoter sequence show strongest promoter activity.
바람직한 로스쿨 설계를 위한 제언 : 사회와 소통하는 로스쿨을 위하여
이상수 서강대학교 법학연구소 2007 서강법학 Vol.9 No.2
Ever since Law School Law has passed on July 3, 2007, most law professors and the staffs of universities have been totally absorbed in designing law school. But the law system itself is so new and strange to Koreans that there comes out a lot of confusions and difficulties in designing desirable law school. To solve the problem this article first shows the core concept of law school by presenting its difference with the current legal education system in terms of its contents, methods. scope, intensity, purpose etc. Then it tries to propose a way to design law school, based upon its new concept. The essence of the proposal is the communication of law school with society. In designing law school, the communication with society should begins at the goal-setting stage. Goal itself should be drawn as a result of the communication with the public or civil society, i.e. judges, prosecutors, lawyers etc. With regard to the specialization of each law school, the designers should consult with the concerned authorities or specialists. The communication with the leader of society, for example, the legislators, local governors or business managers, is also recommended. The communication with society at the operating stage is also important. Specialists outside law school should be given chances to teach in law school. The official relationships with the organizations, governmental or non-governmental, should be set up for the educational purposes. In this respect the research institute in law school is to be run in relation with the need of society and law school education. At the stage of evaluation also, it is necessary to communicate with society. The strict evaluation processes of each lecture and professor should be adopted with the participation of students and civil society as well as by the law school authority. By these kinds of active communication with society, law school can be essential part of social infra-structure which will support the future development of Korean society in the 21st century.
Bacillus subtilis DEAD-box RNA Helicases의 클로닝 및 발현
이상수 배재대학교 자연과학연구소 2017 自然科學論文集 Vol.28 No.1
PCR 방법을 이용하여 Bacillus subtilis DEAD-box RNA helicase 유전자 dead, ydbR, yfmL을 클로닝 하였고 E. coli BL21 (DE3)에 형질전환 하여 T7 프로모터 조절 하에 발현하였다. 이들 DEAD-box RNA helicase들을 Ni-NTA agarose를 사용하여 상용 방법으로 분리하였다. DeaD, YdbR, YfmL RNA helicase들은 저온에서 생체 내의 RNA 구조를 유지시키는 역할을 하며, 서로 30-40%의 아미노산 서열 상동성을 보인다. 이들 유전자의 결손은 저온민감성 생장을 보이는 결과를 확인한 바 있다. 본 논문에서는 클로닝 된 RNA helicase 유전자로부터 순수한 DeaD, YdbR, YfmL RNA helicase들을 분리할 수 있었다. Using the PCR (polymerase chain reaction) dead, ydbR, yfmL genes of Bacillus subtilis coding for DEAD-box RNA helicases have been cloned and expressed under the control of T7 promoter. DEAD-box RNA helicases DeaD, YdbR and YfmL were conventionally purified through Ni-NTA agarose column chromatography. The function of these DEAD-box RNA helicases is keeping RNA structure at low temperature in vivo. Also, the amino acid sequence comparison among these DEAD-box RNA helicases shows 30-40% homologies. It was identified that deletion mutants of dead, ydbR, yfmL are cold sensitive growing. We purified DeaD, YdbR, YfmL RNA helicases from correspondent cloned genes.