초파리 Schneider2 세포와 Chinese hamster ovary-K1 세포에서 Aedes aegypti 5-Hydroxytryptamine7 수용체의 발현비교

이대원 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.2

Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT$\_$7/ receptor of Aedes aegypti, the Aedes 5-HT$\_$7/ receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-Kl cells. The expression of the Aedes 5-HT$\_$7/ receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT$\_$7/ receptor is much more effective in mammalian CHO-K 1 cells and that the Tr-CHO cell line expressing Aedes 5-HT$\_$7/ receptor can be used for synthetic agonist or antagonist candidate screening. 세로토닌 수용체는 세로토닌과 반응하여 세포막의 G단백질을 통해 중개단백질 (adenylyl cyclase, phospholipase C, cGMP phosphodiesterase, ion channel)을 활성화시켜, 이뇨, 기억, 발생 등의 다양한 생리적 반응에 관여한다. 곤충세포인 Schneider2 (S2)와 척추동물 세포인 Chinese hamster ovary (CHO)-Kl에서 Aedes 5-HT$_{7}$ 수용체 유전자 발현을 비교하기 위해, Aedes 5-HT$_{7}$ 수용체 유전자를 형질이입시켰다. 선발된 세포주들(Tr-S2, Tr-CHO)에서 세로토닌 수용체 유전자의 발현은 reverse transcription-PCR, Western blot, immunocytochemistry를 이용하여 확인하였다. 세로토닌 농도증가에 대한 Aedes 5-HT$_{7}$수용체의 기능을 세포 내 cAMP수준을 통해 조사한 결과,Tr-CHO 세포주는 Tr-S2 세포주보다 9배 이상 cAMP수준이 높게 나타났으며, 농도에 의존적이었다. 이 결과는 수용체 유전자가 세포에서 발현되었으나, 세포의 종류와 세포막에 존재하는 G단백질 차이에 따라 중개단백질 활성 차이가 있다는 것을 보여주었다. CHO-Kl 세포에서 Aedes 5-HT$_{7}$ 수용체의 기능이 S2 세포보다 더 효율적이며, Aedes 5-HT$_{7}$ 수용체를 발현하는 Tr-CHO 세포주는 동력제 또는 대립제 검정에 활용될 수 있을 것으로 기대된다. 것으로 기대된다.

착유 자동화를 위한 로봇 착탈 시스템

이대원,최동윤,김현태,이원희,권두중,이승기,한정대 한국축산환경학회 2000 축산시설환경학회지 Vol.6 No.2

A Robotic milking cluster system with the manipulator for an automatic milking system was designed and built for farmer to work easily and comfortably during milking processing. The cluster system was composed of screws, cams and links for power transmission, DC motors, the Quick Basic one-chip microprocessor, the vision system for image processing, and tea-cups. Software, written in Visual C+ and Quick Basic, combined the function of image capture, image processing, milking cluster control, and control into one control. The unit was made to transfer from four fixed points to four teats with four teat-cups. Performance tests of the cluster unit, the fully integrated system, were conducted to attach and detach the teat-cup on the teat of a artificial cow. The transfer programming provided for a teat-cup milking loop during the system starts and comes back the original fixed point at the manipulator of it for milking. It transferred the teat-cup with a success rate of more than 70%. The average time it took ot perform the milking loop was about 20 seconds.

포도수확용 로봇 개발을 위한 영상처리시스템

이대원,김현태,이용국,김동우,성시홍 (사) 한국생물환경조절학회 2001 생물환경조절학회지 Vol.10 No.3

A grape fruit is required for a lot of labor to harvest in time in Korea, since the fruit is cut andgrabbed currently by hand. In foreign country, especially France, a grape harvester has been devel-oped for processing to make wine out of a grape, not to eat a fresh grape fruit. However, a harvesterwhich harvests to eat a fresh grape fruit has not been developed yet. Therefore, this study wasdesigned and constructed to develope a image processing system for a fresh grape harvester. Its devel-opment involved the integration of a vision system along with an personal computer and two cam-eras. Grape recognition, which was able to found the accurate cutting position in three dimension bythe end-effector, needed to find out the object from the background by using two different imagesfrom two cameras. Based on the results of this research the following conclusions were made: Themodel grape was located and measured within less than 1,100 mm from camera center, which meanscenter between two cameras. The distance error of the calculated distance had the distance errorwithin 5mm by using model image in the laboratory. The image processing system proved to be a reli-able system for measuring the accurate distance between the camera center and the grape fruit. Also,difference between actual distance and calculated distance was found within 5 mm using stereo visionsystem in the field. Therefore, the image processing system would be mounted on a grape harvester tobe founded to the position of the a grape fruit.

弗素의 齒牙우식증 豫防效果에 關한 力學的 調査 및 實驗的 硏究

李大源 최신의학사 1975 最新醫學 Vol.18 No.6

Anti-caries effect of fluorine is well known fact and a lot of researches has been conducted. The objective of present study was to ascertain the relationship between fluoride content in the drinking water, and dental caries prevalence in permanent teeth, and fluorine content in the several layers of human enamel. The areas selected in this study were Chowol-Myun Kyungkee -Do, Sangsoo -Myun Chunrabuk-Do and Sannai-Myun Chunrabuk-Do where the fluorine content in drinking water were 0.2~0.3p.p.m. (call AREA A below). Maegok-Myun and Hwangkeum-Myun Chungchungbuk-Do where the fluorine content in drinking water contain 1.1~1.6 p.p.m. were also selected as an area of comparison (call AREA B below). DMF person rate, the fluorine content in the three layers of enamel, mottled index and occurrence of mottled enamel of the children aged 6 to 11 in both sexes were surveyed. The results was as follows: ① The average DMF person rate of the AREA A showed 46.7% in male, 49.4% in female and 48.1% of both. The content of fluorine in the outer third of the enamel was 420p.p.m. , 95p. p. m. in the middle third and 64p. p. m. in the inner third. The average mottled index was revealed 0. 28 and the mottled enamel occurrence was 31.1%. ②The average DMF person rate of the AREA B showed 25.6% in male, 27.2% in female and 26.4% of both. The content of fluorine in the outer third of enamel was 518 p.p.m. , 226 p. p. m. in the middle third and 137 p. p. m. in the inner third. ③Average DMF person rate of the AREA B revealed lower by 45. 1% than the AREA A.

산탄총에 의한 두경부손상 1례

이대원,김종규,김형진,최득록 대한이비인후과학회 1997 대한이비인후과학회지 두경부외과학 Vol.40 No.11

Microcomputer 이용을 위한 사전검토사항

이대원 대한전기학회 1979 전기의 세계 Vol.28 No.6

전자회로의 점검에 테스터만으로 족하였던 진공관이나 트랜지스터의 시대로부터, Oscilloscope를 필수계측기로 사용하는 Digital의 시대를 거쳐서 microcomputer의 시대로 들어선 지금에 microprocessor의 기능을 충분히 활용하는데는 개발을 위한 도구로서의 개발시스템이 절실히 요구된다. Microcomputer화의 문제는 그 hardware를 어떻게 구성하는가의 면과 그것을 hard와 soft의 양쪽의 관점에서 어떻게 효율높게 이용하는가의 두가지 면으로 생각할 수 있다. Frame에 장치된 computer라는 외관을 갖춘 형태가 아니고 화로나 장치시스템속에서 정보처리를 위한 소자로서의 microcomputer를 이용하는 경우에 알아두어야 할 점들을 살피고자 한다.

모바일 웹사이트 통합 개편

이대원 (사)한국인터넷전문가협회 2014 Web Korea Annual Vol.2014 No.-

플라즈마를 이용한 디젤 PM 저감 기술

이대원 한국공기청정협회 2003 空氣淸淨技術 Vol.16 No.2

Circadian Rhythm Disturbances Due to Exposure to Acidified Conditions and Different Photoperiods in Juvenile Olive Flounder (Paralichthys olivaceus)

이대원,송진아,박흥식,최철영 한국해양과학기술원 2021 Ocean science journal Vol.56 No.2

Carbon dioxide (CO2) is being continuously discharged into the atmosphere and is now at a concentration sufficient to cause ocean acidification. In particular, it has been reported that changes in carbonate concentration in seawater by ocean acidification can inhibit olfactory function and predator avoidance ability in fish and affect their circadian rhythm. However, although increased CO2 concentration in seawater is an important environmental factor affecting fish survival, only a few studies have been conducted to evaluate the effect of CO2 and different photoperiods. Therefore, in this study, we investigated changes in the circadian rhythm of juvenile olive flounder (Paralichthys olivaceus) under different light conditions (12 h ligh:12 h dark; constant dark; constant light) and CO2 exposure levels (pH 8.1, 7.8, and 7.5), by analyzing changes in plasma concentrations of Cryptochrome1 and Period2, which are secreted during the day (light conditions), and melatonin, which is secreted at night (dark conditions). CO2 exposure led to phase shifts (temporarily abolished, phase delayed, or reversed) in the rhythm of juveniles. In conclusion, CO2 exposure, along with changes in photoperiods, increases the disturbance in the circadian rhythm of juvenile P. olivaceus.

Bacillus thuringiensis isolates from Korean forest environments

이대원,제연호,Young Ho Koh 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.2

We investigated the distribution, toxicity, morphology, and protein profiles of Bacillus thuringiensis isolates from forests in Korea to isolate naturally occurring novel B. thuringiensis. A total of 170 B. thuringiensis isolates were obtained from 832 samples producing spore and parasporal inclusion bodies. In toxicity tests for lepidopteran,dipteran, and coleopteran insects, 57.6% isolates were toxic only to Lepidoptera, 5.3% were toxic only to Diptera, and 24.1% were toxic to both Diptera and Lepidoptera. The remaining collections (13.0%)were not toxic to the tested insects. The shapes of the parasporal crystals produced in B. thuringiensis isolates were bipyramidal, spherical, ovoid, or irregular. As their toxicities varied with parasporal crystal shape, B. thuringiensis isolates possessing bipyramidal or irregular parasporal crystals were largely toxic to lepidopteran species whereas those producing spherical parasporal crystals were mainly toxic to dipteran species. B. thuringiensis toxic to both dipteran and lepidopteran insects contained 130- and 70-kDa parasporal crystals,whereas B. thuringiensis toxic to lepidopteran insects expressed 130-kDa parasporal crystals. The results suggest that forest areas in Korea are a rich source of B. thuringiensis and need to be further explored to discover novel B. thuringiensis isolates.