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Deoxyribonucleoside의 인산화반응에서 o-chlorophenylphosphorodiazolide의 반응성
李在亮,宋炳洙,李相稷,權英愛 嶺南大學校附設 基礎科學硏究所 1989 基礎科學硏究 Vol.9 No.-
The phosphorylation based on the ‘phosphotriester method’, which proves as one of the useful synthetic methods for DNA segment, was reexamined and improved partially. The reactions between o-chlorophenylphosphorodichloridate and three azolides were conducted to form o-chlorophenylphosphorodiazolides-CPPD I, CPPD T and CPPD Te. With these synthesized diazolides phosphorylation to 3'-oxygen of thymidine was carried out for the observation of stoichiometry. The reaction rate of the phosphorylation was accelerated when the mixed solvent of 5% pyridine/dioxane instead of dioxane alone was employed. The mixed solvent provided increase of yields. This increase may be caused by the catalytic action of the pyridine for the phosphorylation. It was observed that CPPD T was more excellent than CPPD I or CPPD Te for the phosphorylation.
고양이 회장 평활근의 수축력에 미치는 Vanadate와 Ouabain의 작용
이재양,정진섭,김용근,이상호,Lee, Jae-Yang,Jung, Jin-Sup,Kim, Yong-Keun,Lee, Sang-Ho 대한생리학회 1984 대한생리학회지 Vol.18 No.2
Vanadate의 회장 평활근에 대한 수축작용이 Na-K-ATPase를 억제하기 때문인지를 구명하기 위하여 Na-K-ATPase를 억제하는 ouabain과의 작용의 차이를 관찰한 결과 다음과 같은 결론을 얻었다. 1) Ouabain에 의해 나타나는 수축은 2중 peak를 나타내었으나 vanadate 의해서는 단일 Peak만을 보였다. 2) Ouabain에 의한 수축은 atropine$(2{\times]10^{-6}M)$에 의해 강력하게 억제되었으나 vanadate의 작용은 영향을 받지 않았다. 3) Ouabain에 의한 수축은 vanadate에 비해 외부의 $Ca^{++}4농도 및 Ca-길항제에 대해 민감하게 영향을 받았다. 4) 용액내 $Na^+$이 없을때 혹은 고농도의 $K^+$존재하에서 ouabain에 의한 수축반응은 거의 나타나지 않았으나 vanadate에 의한 수축은 영향을 받지 않았다. 5) Vanadate에 의한 수축은 ouabain 존재시에 더욱 증가되었다. 6) 3시간동안 incubation한 결과 vanadate는 ouabain과 달리 세포내 $Na^+$의 농도에 영향을 미치지 못하였다. 이상의 결과로 보아 ouabain과 vanadate는 서로 다른 기전에 의해 회장 평활근에서 수축반응을 유발시키는 것으로 추측된다. Vanadate is a potent inhibitor of Na-K-ATPase. Ouabain, the another specific inhibitor of Na-K-ATPase, induces the contraction in cardiac muscle and smooth muscle. But, some investigators observed the discrepancies between vanadate and ouabain-induced contraction in cardiac muscle. The difference of vanadate and ouabain-induced contraction was investigated in the cat ileal smooth muscle. The following results were obtained. 1) Ouabain-induced contraction was biphasic, but vanadate-induced contraction had one peak. 2) Atropine inhibited ouabain·induced contraction, but did not inhibit vanadate-induced contraction. 3) Changes in external $Ca^{++}$concentration or $Ca^{++}$ antagonists had a greater influence on the contraction induced hy vanadate than by ouabain. 4) Removal of $Na^+$ from incubation medium and high $K^+$ abolished ouabain-induced contraction, but had no effect on vanadate-induced contraction. 5) Vanadate-induced contraction was potentiated in the presence of ouabain. 6) After 3 hrs incubation with vanadate, there was no change in intracellular $Na^+$ concentrations in contrast with ouabain. These results suggest that vanadate contracts ileal smooth muscle through the mechanism different from ouabain, and this is independent of the inhibition of Na-K-ATPase activity.
양승복,이재도 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-
Many small systems, utilizing microprocessors, are now being used in various fields. The need for connecting small system is increasing, which locate within a restricted area such as a single bulding, a small cluster of buldings. Utilizing inexpensive microprocessors, we have studied a structure of the multi-loops network system, intra-loop transmission and inter-loop communication schemes. The multi-loop structure, which consists of a number of loops, has been adopted, therefore some loops can be easily added when the number of nodes increases or the traffic rate becomes higher. Each loop is controlled independently, so that trouble of one loop does not effect the other loops. The control is distributed all over the nodes and there is no centrol loop-controller.
이재도,양승복,정수웅 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-
In this paper, Using a Fuzzy theory an On-Line phoneme recognition procedure of Korean characters is studied. The recognition method is performed by reading the pixel coordinates, finding the feature points, recognizing the stroke, recognizing the phoneme in order. In the course of recognizing the stroke, first the feature points of an inputed stroke is characterized by starting point, end point, and bending point. and then, based on 0, 5, 6, 7, 8, 9, and 10 stroke code among the recognized stroke the distance or direction between base stroke and the next input stroke is used for recognizing the phoneme
이재도,양승복 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-
The study is aimed at developing the parellel processing interpriter for normal order reduction algorithm, which process the functional program. Programmer can develop programs regardless of hardware and parellelism of the algorithm becase functional program is easy to detect parellelism and nees not synchronization aming tasks. After several tast program execated, I see 2.1 and 3.5 times speed-up effect in SUM.L and COND.L program, each other.