巴豆加大黃이 抗腫瘍作用과 自然殺害細胞의 活性에 미치는 영향에 대한 실험적 연구

魯勳政,田炳熏,文九,文錫哉 대한한방종양학회 1996 대한한방종양학회지 Vol.2 No.1

This experiment was designed to study the antitumor effats ad Activity of Natura Killer Cell of semen Tighi plus Rhizoma Rhei. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, Caki-1, LL2, Sarcomal8O, NIH/3T3) after expsure to prebrewed Semen Tiglii plus Rhizoma Rhei water extract 0.l, 0.2, 0.4, 0.8, 1.6mg/ml using in MTT assay. LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. l From the rsult of MTT assay, the cytotoxicity of ST(生巴豆霜), ST+RR(生巴豆霜加大黃) were concentration-de*ndently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR(生巴豆霜加大黃) Was Similar to that of ST(生巴豆霜). 2 From the result of LDH, the cytotoxicity of ST, ST +RR were concentrati -on-dependently increased in both group of the ST ad ST+RR, the cytotoxicity of ST+RR was Simla to that of ST. 3. The antitumor effect on A549 tumor cell from the result of colony foming efficiency showed the inhibitory effect on the growth in both group of the ST and ST+RR, the inhibitory effect on growth was low slightly in the ST+RR. 4. From thc result of SRB assay. the antitumor effect on caki-l tumor cell of ST. ST+RR showed the inhibitory effect on the growth in both group of the ST and ST+RR, the antitumor effect of ST+RR was similar to that of ST. 5. Nedian survival time and increased life span were increased slightly in both group of the ST and ST+RR. 6 The inhibitory effect on the growth of Sarcoma l80 and Lewis lung carcinoma tumor cell were increased slightly in both group of thc ST and ST+RR. 7. The itchyity of NK cell was increased in the ST+RR.

肛腸療法의 原理, 常用方法및 臨床應用에 對한 文獻的考察

魯勳政,文九 한국전통의학연구소 1999 한국전통의학지 Vol.9 No.1

Anal Therapy is taken valid compound of herb med and has many kinds of treatments. As one of the anal therapy, retention enema is most common and useful way. This study was performed in order to literatural basements of clinical treatments. we had cleared manifest about the origin and literatural basement of anal therapy, methods of management, classification of indication, clinical application and so forth. The results were summerised as follows. 1. It explained relationships between anus and Ojang-yukbu(五臟六腑), Sibie-geongmaek(十二經脈). But in fact the origin of anal therapy is Milgeon-dobub(蜜煎導法) and Jedamjib-dobub(猪膽汁導法) in Sanghan-jabbyungron(傷寒雜病論) of Han Dynasty. 2. The effect of anal therapy can be reached to the destination through theory of the organism which is called Jung-chei theory(整體論) and local medical action. We can find a little in Naegeong(內經), which is basement of Hang-jang therapy. 3. Anal Therapy have Kwanjangbub(灌腸法), Guhangbub(灸肛法), Dohangbub(塗肛法), Hoonhangbub(熏肛法), Saekhangbub(塞肛法), Youkhangbub(浴肛法), Jwajeombub(坐墊法) e.t.c. 4. Anal Therapy is available when person can't taken by mouth. Its benefits are fast effect and low side effect about liver because it is not taken trough liver mostly. 5. Anal Therapy has been used for the treatment of diseases such as stroke, high fever, pneumonia, ulcerative colitis, prostatitis, renal failure, pelvic inflammation, cancer and so on.

肛腸治療의 原理, 常用方法 및 臨床應用에 對한 文獻的 考察

노훈정,문구,Roh, Hun-Jung,Moon, goo 대한한방안이비인후피부과학회 1999 한방안이비인후피부과학회지 Vol.12 No.2

Anal Therapy is taken valid compound of herb med and has many kinds of treatments. As one of the anal therapy, retention enema is most common and useful way. This study was performed in order to literatural basements of clinical treatments. we had cleared manifest about the origin and literatural basement of anal therapy, methods of management, classification of indication, clinical application and so forth. The results were summerised as follows. 1. It explained relationships between anus and Ojang-yukbu(五臟六腑), Sibie-geongmaek(十二經脈). But in fact the origin of anal therapy is Milgeon-dobub(蜜煎導法) and Jedamjib-dobub(猪膽汁導法) in Sanghan-jabbyungron(傷寒雜病論) of Han Dynasty. 2. The effect of anal therapy can be reached to the destination through theory of the organism which is called Jung-chei theory(整體論) and local medical action. We can find a little in Naegeong(內經), which is basement of Hang-jang therapy. 3. Anal Therapy have Kwanjaogbub(灌腸法), Guhangbub(灸肛法), Dohangbub(塗肛法), Hoonhangbub(熏肛法), Saekhangbub(塞肛法), Youkhangbub(浴肛法), Jwajeombub(坐점法) e.t.c. 4. Anal Therapy is available when person can't taken by mouth. Its benefits are fast effect and low side effect about liver because it is not taken trough liver mostly. 5. Anal Therapy has been used for the treatment of diseases such as stroke, high fever, pneumonia, ulcerative colitis, prostatitis, renal failure, pelvic inflammation. cancer and so on.

파두가대황(巴豆加大黃)이 항종양작용(抗腫瘍作用)과 자연살해세포(自然殺害細胞)의 활성(活性)에 미치는 영향에 대한 실험적 연구

노훈정,전병훈,문구,문석재,No, Hun-Jeong,Jeon, Byeong-Hun,Mun, Gu,Mun, Seok-Jae 대한암한의학회 1996 大韓癌韓醫學會誌 Vol.2 No.1

This experiment was designed to study the antitumor effects and Activity of Natural Killer Cell of semen Tiglii plus Rhizoma Rhei. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, Caki-1, LL2, Sarcoma 180, NIH/3T3) after exposure to prebrewed Semen Tiglii plus Rhizoma Rhei water extract 0.1, 0.2, 0.4, 0.8, 1.6mg/ml using in MTT assay, LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. From the result of MTT assay, the cytotoxicity of ST(生巴豆霜), ST+RR(生巴豆霜加大黃) were concentration-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR(生巴豆霜加大黃) was similar to that of ST(生巴豆霜). 2. From the result of LDH, the cytotoxicity of ST, ST +RR were concentrati -on-dependently increased in both group of the ST and ST+RR, the cytotoxicity of ST+RR was similar to that of ST. 3. The antitumor effect on A549 tumor cell from the result of colony forming efficiency showed the inhibitory effect on the growth in both group of the ST and ST+RR, the inhibitory effect on growth was low slightly in the ST+RR. 4. From the result of SRB assay, the antitumor effect on caki-1 tumor cell of ST, ST+RR showed the inhibitory effect on the growth in both group of the ST and ST+RR, the antitumor effect of ST+RR was similar to that of ST. 5. Median survival time and increased life span were increased slightly in both group of the ST and ST+RR. 6. The inhibitory effect on the growth of Sarcoma 180 and Lewis lung carcinoma tumor cell were increased slightly in both group of the ST and ST+RR. 7. The activity of NK cell was increased in the ST+RR.

소적보중환(消積保中丸)의 항종양효과(抗腫瘍效果)에 대한 실험적(實驗的) 연구(硏究)

노훈정,전병훈,문구,문석재,No, Hun-Jeong,Jeon, Byeong-Hun,Mun, Gu,Mun, Seok-Jae 대한암한의학회 1996 大韓癌韓醫學會誌 Vol.2 No.1

The sprig of Sojekbojungwhan(消積保中丸) has been used for curing as a traditional medicine without any experimental evidence to support the rational basis for their clinical use. This experiment was carried out to evaluate the possible therapeutic or antitumoral effects of Sojekbojungwhan extract against cancer, and to study some mechanisms responsible for its effect. The cytotoxic and antitumor effects were evaluated on human cell lines(A549, hep3B, Caki-1, Sarcoma 180) after esposure to Sojekbojungwhan extract using in ILS, colony forming efficiency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. As a result of exposure to Sojekbojungwhan extract, the proliferation of A549, hep3B, Caki-1, good correlations were shown from the results of SRB assay and those of clogenetic assay. 2. The oral administration of Sojekbojungwhan extact showed significant effects of increase of MST(mean survival time) and ILS(increased life span) depending on the increasing concentration. 3. Against squamous cell carcinoma induced by MCA, Sojekbojungwhan decreased not only the frequency of tumor production but also the number and weight of tumors per tumor bearing mice(TBM). Sojekbojungwhan also significantly suppressed the development of 3LL cell-implanted tumors by frequency and their size, and some developed tumors were regressed by the continuous treatment of Sojekbojungwhan extract into TBM. 4. Sojekbojungwhan extract also increased NK cell activities. According to the above results, it could be suggested that Sojekbojungwhan extract has some antitumor effects.

백화사설초(白花蛇舌草) 메탄올 추출물(抽出物)의 항종양(抗腫瘍) 효과(效果) 및 항암(抗癌) 기전(機轉)에 관(關)한 연구(硏究)

노훈정,문구,문석재,원진희,문영호,박래길,No, Hoon-Jeong,Moon, Gu,Moon, Seok-Jae,Won, Jin-Hee,Moon, Young-Ho,Park, Rae-Gil 대한암한의학회 2000 大韓癌韓醫學會誌 Vol.6 No.1

Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to $0.4{\mu}g$) and periods (6 to 30 hr) of $H_2O$ and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with $200{\mu}g/ml$ of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with $200{\mu}g/ml$ of each extract for 16 hr. Then, cells were treated with Hoechst dye 33342 and observed by fluorescence microscopy. Cells were treated with various doses of each for 12 hr and $100{\mu}g/ml$ of methanol extract for various periods. Lysate from the cells used to measure the activity of Caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively. Cells were treated with $200{\mu}g/ml$ of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with $32^p-ATP$ and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by using PhosphoImage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of $NF-{\kappa}B$. Transcriptional activation of ${\kappa}B$ was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at $CO_2$ incubator for 6 days. The number of colony was counted under light microscopy ($\time100$). Results: The death of HL-60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL-60 cells. In addition, it was shown nucleus chromatin condensation and fragmentation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspase 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, $NF-{\kappa}B$ was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator $NF-{\kappa}B$, In addition, our results also suggest that methanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming

消積保中丸의 抗腫瘍效果에 대한 實驗的 硏究

魯勳政,田炳熏,文九,文錫哉 대한한방종양학회 1996 대한한방종양학회지 Vol.2 No.1

The sprig of Sojekbojungwhan(消積保中丸) has been used for curing as a traditional medicine without any experimental evidence to support the rational basis for their clinical use. This experiment was carried out to evahate thc possible titerapeutic or antitumoral effects of Sojekbojungwhan extract against cancer, and to study some mechanisms responsible for its effect. The cytotoxic md antitumor effects weer evaluated on human cell lines(A549, hep3B, Caki-l. Sarcoma 180) after esposure to Sojekbojungwhan extract using in ILS, colony forming efficiency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. l. As a msult of exposure to Sojekbojungwhan extract, the proliferation of A549. hep3B. Caki-1, good correlations were shown from the results of SRB assay and tho-of clogenetic assay. 2. The oral administration of Sojekbojungwhan extact showed significant effects of increase of MST(mean survival time) and MST(mean survival time) and ILS(increased life span) depending on the increasing concentration. 3. Against squamous cell carcinoma induced by MCA. Sojekbojungwhan decreaed not only the frc-quency of tumor production but also the number and weight of tumors per tumor bearmg mice(TBM). Sojekbojungwhan also significantly suppresscd the development of 3LL cell-implanted tumors by frequency and their size. ad some developed Iumors were regressed by the continuous treatment of Sojekbojungwhan extract into TBM. 4. Sojekbojungwhan extract also increased NK cell activities. According to the above results, it could be suggested that Sojekbojungwhan extract has some antitumor effects.

白花蛇舌草 메탄올 抽出物의 抗腫瘍 效果 및 抗癌 棋戰에 關한 硏究

魯勳政,文九,文錫哉,元秦熹,文永昊,朴來佶 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to 0.4㎍) and periods (6 to 30 hr) of H_2O and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with 200㎍/ml of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with 200㎍/ml of each extract for 16hr.Then, cells were treated with various doses of each extract for 12 hr and 100㎍/ml of methanol extract for various periods. Lysate from the cells used to measure the activity of caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively, Cells were treated with 200㎍/ml of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with 32^p-ATP and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by suing Phosphoimage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of NF-kB was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at CO_2 incubator for 6 days. The number of colony was cunted under light microscopy (×100). Results: The death of HL_60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL_60 cells. In addition, it was shown nucleus chromatin condensation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspaxe 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, NF-kB was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator NF-κB, In addition, our results also suggest that methanol exthanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming effciency through cleavage of Bc12 as well as induction of Bax.

肛腸질法의 原理, 常用方法 및 臨床應用에 對한 文獻的 考察

魯勳政,文九 한국전통의학연구소 1999 한국전통의학지 Vol.9 No.1

Anal Therapy is taken valid compound of herb med and has many kinds of treatments. As one of the anal therapy, retention enema is most common and useful way. This study was performed in order to literatural basements of clinical treatments. we had cleared manifest about the origin and literatural basement of anal therapy, methods of management, classification of indication, clinical application and so forth. The results were summerised as follows. 1. It explained relationships between anus and Ojang-yukbu(五臟六腑), Sibie-geongmaek(十二經脈). But in fact the origin of anal therapy is Milgeon-dobub(密煎導法) and Jedamjib-dobub(猪膽汁導法) in Sanghan-jabbyungron(傷寒雜病論) of Han Dynasty. 2. The effect of anal therapy can be reached to the destination through theory of the organism which is called Jung-chei theory(整體論) and local medical action. We can find a little in Naegeong(內經), which is basement of Hang-jang therapy. 3. Anal Therapy have Kwanjangbub(灌腸法), Guhangbub(灸肛法), Dohangbub(塗肛法), Hoonhangbub(熏肛法), Saekhangbub(塞肛法), Youkhangbub(浴肛法), Jwajeombub(坐墊法) e.t.c. 4. Anal Therapy is available when person can't taken by mouth. Its benefits are fast effect and low side effect about liver because it is. not taken trough liver mostly. 5. Anal Therapy has been used for the treatment of diseases such as stroke, high fever, pneumonia, ulcerative colitis, prostatitis, renal failure, pelvic inflammation, cancer and so on.

전기자동차 배터리 및 동력제어시스템 설계

임정헌(J. H. Im),임경준(K. J. Im),조진우(J. W. Cho),이도근(D. K. Lee),유오성(O. S. Yoo),권우진(W. J. Kwon),김현우(H. W. Kim),김태승(T. S. Kim),이우용(W. Y. Lee),오영민(Y. M. Oh),김태완(T. W. Kim),노훈정(H. J. Nho),김동훈(D. H. Kim),이창진(C. 한국기계가공학회 2023 한국기계가공학회 춘추계학술대회 논문집 Vol.2023 No.12