대장균에서 무작위 샤인-달가노 서열이 소성장호르몬 유전자 발현에 미치는 영향

나경수,나경수,백형석,이용세 한국생명과학회 2000 생명과학회지 Vol.10 No.4

In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.

Condylar bony changes in patients with temporomandibular disorders: a CBCT study

나경수 대한영상치의학회 2012 Imaging Science in Dentistry Vol.42 No.4

Purpose: Diagnosis of osteoarthritis most commonly depends on clinical and radiographic findings. The present study attempted to observe the bony changes in temporomandibular joint (TMJ) patients from all age groups. Materials and Methods: The first-visit clinical records and cone beam computed tomography (CBCT) data of 440 TMJs from 220 consecutive TMJ patients were reviewed retrospectively. Results: The most frequent condylar bony change observed was sclerosis (133 joints, 30.2%) followed by surface erosion (129 joints, 29.3%), flattening of the articular surface (112 joints, 25.5%), and deviation in form (58 joints, 13.2%), which included 33 TMJs in a cane-shape, 16 with a lateral or medial pole depression, 6 with posterior condylar surface flattening, and 3 with a bifid-shaped condyle. Fifty-three joints (12.0%) showed hypoplastic condyles but only 1 joint showed hyperplasia. Osteophyte was found in 35 joints (8.0%) and subcortical cyst in 24 joints (5.5%), 5 of which had surface erosion as well. One hundred nineteen joints (27.0%) had only one kind of condylar bony change, 66 joints (15.0%) had two, 52 joints (11.8%) had three, 12 joints (5.0%) had four, and 6 joints (1.4%) had five kinds of condylar bony changes at the same time. Eighty-five (65.9%) of 129 joints with surface erosion had pain recorded at the chief complaint. Conclusion: With more widespread use of CBCT, more specific or detailed guidelines for osteoarthritis are needed. Purpose: Diagnosis of osteoarthritis most commonly depends on clinical and radiographic findings. The present study attempted to observe the bony changes in temporomandibular joint (TMJ) patients from all age groups. Materials and Methods: The first-visit clinical records and cone beam computed tomography (CBCT) data of 440 TMJs from 220 consecutive TMJ patients were reviewed retrospectively. Results: The most frequent condylar bony change observed was sclerosis (133 joints, 30.2%) followed by surface erosion (129 joints, 29.3%), flattening of the articular surface (112 joints, 25.5%), and deviation in form (58 joints, 13.2%), which included 33 TMJs in a cane-shape, 16 with a lateral or medial pole depression, 6 with posterior condylar surface flattening, and 3 with a bifid-shaped condyle. Fifty-three joints (12.0%) showed hypoplastic condyles but only 1 joint showed hyperplasia. Osteophyte was found in 35 joints (8.0%) and subcortical cyst in 24 joints (5.5%), 5 of which had surface erosion as well. One hundred nineteen joints (27.0%) had only one kind of condylar bony change, 66 joints (15.0%) had two, 52 joints (11.8%) had three, 12 joints (5.0%) had four, and 6 joints (1.4%) had five kinds of condylar bony changes at the same time. Eighty-five (65.9%) of 129 joints with surface erosion had pain recorded at the chief complaint. Conclusion: With more widespread use of CBCT, more specific or detailed guidelines for osteoarthritis are needed.

호남지역 전통문화의 現前化를 위한 활동 모색

나경수 전남대학교 호남학연구원 2006 호남문화연구 Vol.0 No.39

Osteomas of the craniofacial region

나경수 대한영상치의학회 2011 Imaging Science in Dentistry Vol.41 No.3

Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones. Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones.

珍島의 려제고

羅景洙 全南大學校 湖南文化硏究所 1987 호남학 Vol.17 No.-

잊혀진 그대를 위한 레퀴엠

나경수,Na, Gyeong-Su 기술표준원 2007 기술표준 Vol.62 No.-

광양시 일대 조사보고서 : 세시풍속

나경수,송준,김성신 남도민속학회 2007 남도민속연구 Vol.14 No.-

아담신화와 무위자연론의 비교 : 문화의 개념 정립을 위한 시고

羅景洙 全南大學校 人文科學硏究所 1993 용봉인문논총 Vol.22 No.-

A common feature that we can find out from comparing the Myth of Adam with the Doctrine of Inactive Nature is that both have a same idea about Knowledge Both the Garden of Eden and the World of Inactive Nature are places where Knowledge connot maintain its position. On the contrary, the Lost Paradise and the earthly world are places where we seek after Knowledge, and are supported by Knowledge. Lao-tzu said that the best goodness is letting matters their natural courses. That idea is comparable to the idea of Myth of Adam: 'We can live permanently only by not eating the fruit of the Tree of Knowledge' The Lost Paradies is a cultural life or cultural place in the Myth of Adam, because it is the place where we must work and produce something useful. That is to say, the fruit of the Tree of Life is a symbol of permanent life, and the fruit of the Tree of Knowledge is a symbol of Knowledge. So we can conclude that the places, where we can be workless and unproductive, is the world of Inactive Nature, while the place where we must work and produce something is artificial and cultural world.

여수시 삼산면 초도의 세시풍속

나경수,김숙희,이용식 남도민속학회 2011 남도민속연구 Vol.22 No.-

운주사전설의 연구

나경수 한국언어문학회 1997 한국언어문학 Vol.38 No.-