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질 내 공생세균 Dermabacter vaginalis 유래 항진균 대사체의 분리 및 합성
황인현,소재성,권강무,이문선,김대근 한국생약학회 2022 생약학회지 Vol.53 No.3
Vaginal microbiome deeply influences vaginal health via production of messenger molecules. The healthy vaginal pH ranges between 3.5 and 4.5. However, dysbiosis of vaginal microbiome increases the pH level, leading to the incidence of vaginitis. The commensal vaginal bacterium Dermabacter vaginalis―which was isolated from the vaginal fluid of a Korean female―was incubated in acidic and neutral pH to simulate healthy and vaginitis conditions, respectively. The chemical profiles of the two different cultures were compared using HPLC. The compound showing distinctive difference between the two sets of data was presumed to be a chemical messenger, which was identified as cyclo(L-pro-L-met) by analysis of NMR, MS, and specific rotation data. Synthesis was achieved in three steps (overall yield 15%), enabling structure confirmation and antimicrobial evaluation against vaginal pathogens. Cyclo(L-pro-L-met) showed antifungal activity against Candida albicans, a major cause of vulvovaginal candidiasis.
Complete NMR assignments of undegraded asterosaponins from Asterias amurensis
황인현,Roshan Kulkarni,양민혜,조수진,Wei Zhou,이상명,장태수,정길생,장현욱,나민균 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.10
Four asterosaponins, thornasteroside A (1),versicoside A (2), anasteroside B (3), and asteronylpentaglycosidesulfate (4), were isolated from the predatorystarfish Asterias amurensis Lu¨tken. Unlike previous studiesfocusing on structure elucidation by degradation of thecomplex saponin molecules, complete nuclear magneticresonance (NMR) assignment for the intact molecules wasaccomplished using 600 MHz high magnetic field NMR. The complete set of NMR assignments can help in thestructure elucidation of asterosaponins isolated in lowyields without resorting to chemical degradation. Furthermore,this approach can be extended to other complexsteroidal saponins, which may accelerate the discovery ofbioactive secondary metabolites from this invasive starfishspecies.
박서영,황인현,김지선,정영화,송규용,나민균,오상택 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5
Abnormal up-regulation of b-catenin expressionis associated with the development and progression ofmultiple myeloma and is thus a potential therapeutic target. Here, we screened cell-based natural compounds andidentified smenospongidine, a metabolite isolated from amarine sponge, as an antagonist of the Wnt/b-catenin signalingpathway. Smenospongidine promoted the degradationof intracellular b-catenin that accumulated via Wnt3aor 6-bromoindirubin-30-oxime, an inhibitor of glycogensynthase kinase-3b. Consistently, smenospongidine downregulatedb-catenin expression and repressed the levels ofb-catenin/T cell factor-dependent genes such as axin2,c-myc, and cyclin D1 in RPMI-8226 multiple myelomacells. Smenospongidine suppressed proliferation andsignificantly induced apoptosis in RPMI-8266 cells. Inaddition, smenospongidine-induced b-catenin degradationwas mediated by up-regulating CCAAT/enhancer-bindingprotein homologous protein (CHOP). These findings indicatethat smenospongidine exerts its anti-proliferativeactivity by blocking the Wnt/b-catenin signaling pathwayand may be a potential chemotherapeutic agent againstmultiple myeloma.
이정인,황인현,김장훈,김미애,황재삼,김영호,나민균 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.9
Edible insects have been reported to producemetabolites showing various pharmacological activities,recently emerging as rich sources of health functional food. In particular, the larvae of Protaetia brevitarsis seulensis(Kolbe) have been used as traditional Korean medicines fortreating diverse diseases, such as breast cancer, inflammatorydisease, hepatic cancer, liver cirrhosis, and hepatitis. However, only few chemical investigations werereported on the insect larvae. Therefore, the aim of thisstudy was to discover and identify biologically activechemical components of the larvae of P. brevitarsis seulensis. As a result, a quinoxaline-derived alkaloid (1) wasisolated, which was not reported previously from naturalsources. In addition, other related compounds (2, 4–10, 15,16) were also encountered for the first time from the larvae. The structures of all the isolated compounds were establishedmainly by analysis of HRESIMS, NMR, andelectronic circular dichroism data. Compound 5 exhibitedinhibition of tyrosinase with IC50 value of 44.8 lM.
이정인,황인현,장태수,나민균 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.3
Determination of bioactive chemicals in Acer tegmentosum is essential for its industrial application. Twelve phenolic compounds were isolated from A. tegmentosum. Specific seven compounds in the plant were simultaneously analyzed by high performance liquid chromatography-UV. The content of bioactive chemicals in the branch extract was determined to be 3.38 mg/g of (−)-epigallocatechin, 66.95 mg/g of salidroside, 39.61 mg/g of tyrosol, 17.04 mg/g of catechin, 7.39 mg/g of 1,2,4-tri-O-galloyl-β-d-glucose, 8.88 mg/g of 6-O-galloylsalidroside, and 4.61 mg/g of scopoletin. The method was validated with linearity (R 2 > 0.9947), as well as intra- and inter-day precision (0.07–2.77%) and accuracy (>97.0%). This analytical method is expected to be applied for standardization of dietary food containing A. tegmentosum.
이정인,황인현,장태수,나민균 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.12
Determination of bioactive chemicals in Acer tegmentosum is essential for its industrial application. Twelve phenolic compounds were isolated from A. tegmentosum. Specific seven compounds in the plant were simultaneously analyzed by high performance liquid chromatography‐UV. The content of bioactive chemicals in the branch extract was determined to be 3.38 mg/g of (−)‐epigallocatechin, 66.95 mg/g of salidroside, 39.61 mg/g of tyrosol, 17.04 mg/g of catechin, 7.39 mg/g of 1,2,4‐tri‐O‐galloyl‐β‐d‐glucose, 8.88 mg/g of 6‐O‐galloylsalidroside, and 4.61 mg/g of scopoletin. The method was validated with linearity ( R 2 > 0.9947), as well as intra‐ and inter‐day precision (0.07–2.77%) and accuracy (>97.0%). This analytical method is expected to be applied for standardization of dietary food containing A. tegmentosum.
이동성,황인현,임남경,정길생,나민균 한국생약학회 2015 Natural Product Sciences Vol.21 No.4
Sesquiterpene-quinone is a class of secondary metabolites frequently encountered from marine sponge. The present study was designed to examine the anti-inflammatory action of sponge-derived dactyloquinone B (DQB) and cyclospongiaquinone-1 (CSQ1) mixture using lipopolysaccharide (LPS)-induced inflammatory responses. We measured the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-a), interleukin-1b (IL-1b), and interleukin-6 (IL-6) and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. TNF-a, IL-1b, and IL-6 production, which increased by treatment with LPS, were significantly inhibited by DQB and CSQ1 mixture. It also decreased the production of NO production, and iNOS and COX-2 expression. Furthermore, it reduced 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema of ICR mice. These results demonstrate that sesquiterpene-quinone, DQB and CSQ1 mixture, might serve as a chemical pipeline for the development of anti-inflammatory agent.