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반응표면분석법을 이용한 Lactobacillus 균주 배양조건의 통계적 최적화
황영민,이희석 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.5
프로바이오틱스 제품에 대한 수요가 지속적으로 증가하 고 있으며, Lactobacillus 균주가 가장 대중적인 프로바이 오틱스로 널리 사용되고 있다. 프로바이오틱스는 기준에 적합한 균수의 확보가 중요하며 제조원가나 시간 등을 낮 추기 위해 배양법의 개발이 필요하므로 Lactobacillus 생 산을 위한 배양 조건이 최적화되었다. 반응표면방법론에 의한 통계적 최적화에서 반응 변수에 영향을 미치는 독립 변수의 최적 조건은 Lactobacillus acidophilus의 경우 22.55 시간(배양시간), 25o C(배양온도), 3.41%(프리바이오틱스 농 도); Lactiplantibacillus plantarum의 경우 24시간, 30.86o C, 2.00%; Lacticaseibacillus rhamnosus의 경우 66.67시간, 35o C, 3.41%이었다. Lactobacillus의 최적 배양조건은 예측 한 결과와 실제 결과가 밀접하게 일치하는 것을 확인하였 다. 이러한 데이터는 수율 높은 Lactobacillus를 생산하는 데 중요한 포인트를 제공할 것이다. The demand for probiotic products has been steadily increasing, and Lactobacillus strains are widely used and are currently the most popular probiotics. Optimizing culture conditions for Lactobacillus production for use as probiotics will enhance their profitability by reducing production costs and time. Statistical analysis using response surface methodology revealed the following optimal sets of independent variables: 22.55 h (cultivation time), 25°C (cultivation temperature), and 3.41% (w/w, prebiotics concentration) for Lactobacillus acidophilus; 24 h, 30.86°C, and 2% (w/w) for Lactiplantibacillus plantarum; 66.67 h, 35°C, and 3.41% (w/w) for Lacticaseibacillus rhamnosus. Actual outcomes using predicted optimal conditions for Lactobacillus strains have been confirmed to closely match predicted results. This study will provide valuable guidelines for high yield Lactobacillus production.
황영민,이희석 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.3
The environmental conditions were optimized to produce the enniatin H, I, and MK1688 by Fusarium strain on cereal grain exhibiting anti-carcinogenic potential against MES-SA (human uterine sarcoma cell line), HCT15 (human colorectal carcinoma cancer cell line), and their multidrug resistance sublines. From the statistical optimization by response surface methodology, the optimal condition of independent variables affecting the response variables were 20.85 °C (temperature), 46.85% (w/w, initial moisture content), and 18.42 days (growth time) for ENN H; 23.31 °C, 44.15% (w/w) and 17.23 days for ENN I; 23.08 °C, 43.97% (w/w) and 17.06 days for ENN MK1688. In case of cytotoxic effects, ENNs significantly suppressed growth of cancer cell lines without multidrug resistance, and ENN I inhibited growth of cancer cell lines most strongly. These data will provide valuable point to produce the cyclic hexadepsipeptide exhibiting anti-carcinogenic potential from Fusarium strains.
황영민,이진실,안하정,오혜련,성대경,태기융,최원일 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
We previously developed an injectable levan-based hydrogel as a temporary dermal filler. However, thelongevity and collagen stimulation effect of the levan-based hydrogel were limited. Herein, we preparedhydroxyapatite (HAp)-embedded levan composite hydrogel as a long-acting dermal filler for enhancingin vivo collagen production and anti-wrinkle efficacy. HAp amount (0.1–5 wt%) in the levan hydrogelwas optimized for stability, safety, and efficacy. Increasing the HAp amount decreased the in vitro stabilityand elastic modulus, but the composite hydrogel structure was still maintained. Furthermore, thecomposite hydrogel showed improved cell proliferation stimulation compared to the bare levan hydrogelin human dermal fibroblast cells. In vivo stability of the composite hydrogel was enhanced in contrast tothe in vitro result, showing stable residence of the composite hydrogels with 0.5 or 1 wt% HAp over8 weeks. Moreover, the excellent anti-wrinkle efficacy of composite hydrogel with 1 wt% HAp was maintainedduring 8 weeks and it showed the highest collagen production. Thus, HAp-embedded levan compositehydrogel showed potential as a dermal filler with easy injectability, biocompatibility, and longactinganti-wrinkle efficacy.
황영민,석창목,박상훈 한국공간구조학회 2003 한국공간구조학회지 Vol.3 No.4
In case of rectangular latticed pattern which shearing rigidity is very small, it has a concern to drop Buckling-strength considerably by external force. So, by means of system to increase buckling-strength, there is a method of construction that lattice of dome is reinforced by braced member. In a case like this, shearing rigidity of braced member increase buckling-strength of the whole of structure and can be designed economically from the viewpoint of practice. Therefore, this paper is aimed at investigating how much does rigidity of braced member united with latticed member bearing principal stress of dome increase buckling-strength of the whole of structure. the subject of study is rectangular latticed domes that are a set of 2-way lattice dome which grid is simple and number of member gathering at junction is small. Analysis method is based on FEM dealing with the geometrically nonlinear deflection problems.