Molecular Typing of Clinical Cryptococcus gattii Isolates in Korea

황수명 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.2

Cryptococcus gattii causes life-threatening yeast infection in the pulmonary and central nervous systems of humans and animals, and traditionally has been considered to restrict into the tropical and subtropical areas. Despite rare incidence of cryptococcosis caused by C. gattii in Korea, three strains of C. gattii isolated from cryptococcosis patients between 1993 and 2010 were identified. To determine the genetic diversity, 3 strains of C. gattii were typed using PCR fingerprinting with primer M13 and the restriction fragment length polymorphism (RFLP) of orotidine monophosphosphate pyrophosphorylase (URA5) gene. All isolates were identified as serotype B and MATα mating type. The molecular types of each strain, on the other hand, turned out to be distinct belonging to VGI, VGII or III types, respectively. Although the travel histories of the patients were not available, clinical C. gattii strains isolated in Korea may represent the diverse molecular types existing worldwide.

한국의 임상과 자연환경에서 분리된 Cryptococcus neoformans의 혈청형과 효소생성능

황수명,오광석,이경원,Hwang, Soo-Myung,Oh, Kwang-Seok,Lee, Kyung-Won 한국미생물학회 2006 미생물학회지 Vol.42 No.4

Fifty eight Cryptococcus neoformans strains isolated from clinical and environmental sources in Korea were examined for their serotypes and extracellular enzyme activities. Among the 51 strains isolated from clinical sources, 48 strains were serotype A (94.1%), 2 strains were serotype B (3.92%), and 1 strain was serotype D (1.96%). All seven environmental strains isolated from pigeon excreta were identified as serotype A. All isolates of C. neoformtans were positive for the production of extracellular proteinase and phospholipase. In the API-ZYM system, all fifty eight isolates produced alkaine phosphatase, esterase C4, esterase lipase: C8, leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrase, $\alpha$-glucosidase and $\beta$-glucosidase. Thirty nine isolates (67.2%) of C. neoformans produced N-acetyl-$\beta$-glucosidase. Two isolates, serotype B, and B only one serotype A produced $\beta$-glucuronidase. Analysis of enzymatic profiles to 21 enzymes revealed four biotypic patterns among the 58 strains. The enzymatic patterns of C. neoformans isolated from clinical and environmental sources represented a significant relationship with the serotypes. 한국의 임상검체와 자연환경에서 분리된 Cryptococcus neoformans 58주에 대한 혈청형과 세포외효소 생성능에 관한 셍물학적 특성을 조사하였다. 환자로부터 분리된 임상균주 51주 중 48주는 혈청형 A (94.1%) 였으며 2주는 혈청형 B (3.92%),그리고 나머지 1주는 혈청형 D (1.96%)였다. 자연환경에서 분리된 7주는 비둘기 분변에서 분리된 것들이며 모두 혈정형 A였다. 모든 균주는 proteinase와 phnospholipase를 생성하였고, 또한 API-ZYM system을 이용한 19종류의 효소생성능 시험에서는 alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, $\alpha$-glucosidase, 그리고 $\beta$-glucosidase를 생성하였으나, N-acetyl-$\beta$-glucosarninidase는 39주 (67.2%)에서만 생성하였다. 혈청형 B로 동정된 2주와 혈청형 A로 동정된 균주중 1주는$\beta$-glucuronidase를 생성하였다. 본 연구에 사용된 총21종류의 효소 생성능 시험을 기초로 하여 생물형을 구분하였는데, 모두 4가지의 유형을 나타내었고, 또한 임상과 환경균주에서 혈정형과 생물형 특성간의 유의한 상관성를 나타내었다.

부산지역에서 분리된 Staphylococcus aureus의 Coagulase 혈청형 변화

황수명,김태운,Hwang, Soo-Myung,Kim, Tae-Un 한국미생물학회 2007 미생물학회지 Vol.43 No.4

1994년에서 2005년 사이 부산지역의 임상검체와 건강인의 비강에서 분리된 Staphylococcus aureus의 coagulase 혈청형의 변화를 조사하였다. 총 715균주에서 methicillin 내성 S. aureus (MRSA)는 임상균주로 408주였으며, 나머지 307주는 methicillin 감수성(MSSA) 균주로서, 8종(I-VIII)의 coagulase 혈청형이 분리되었다. MRSA 균주에서 가장 분리율이 높은 coagulase 혈청형은 II형(54.3%, 222/408)이었으며, 다음으로 IV형(24.7%), III형(10.9%), V형(5.2%)순으로 나타났으며, MSSA 균주에서는 VII형(30.9%, 95/307), IV형(22.2%), V형(22.2%), II형(7.1%)의 순으로, 균주간에 큰 차이를 나타내었다. 분석 연도별 coagulase 혈청형을 비교한 결과 큰 변화가 관찰되었다. MRSA 균주에서 혈청형 V형은 1997년까지 분리되지 않았으나, 2005년에는 18.5% (20/108)로 증가되었고, III형은 1994년 27.0% (31/115)에서 2005년 0.9% (1/108)로 감소되었다. 또한 MSSA 균주에서도 유사한 경향을 나타내었으나, II형의 경우 2005년도 균주에서는 분리되지 않았다. 이와 같은 결과에서 S. aureus의 coagulase 항원성 전이에 따른 혈청형의 변화를 확인하였다. We analyzed the phenotypic changes in coagulase serotype of S. aureus isolated from clinical sources and nasal cavities of healthy persons, $1994{\sim}2005$. A total of 715 isolates, 408 methicillin resistant S. aureus (MRSA) from clinical sources and 307 methicillin-susceptible S. aureus (MSSA), were classified into eight coagulase sero-types, I to VIII. The most prevalent serotype in MRSA was type II (54.3%, 222/408) and followed IV (24.7%), III (10.9%), and V (5.2%), whereas the majorities in MSSA were type VII (30.9%, 95/307), IV (22.2%), V (22.2%) and II (7.1%). Among the isolates collected periods, significant changes of coagulase serotypes in both strains were observed. In MRSA strains, the serotype V was not detected until 1997, but rapidly increased to 18.5% (20/108) in 2005, and the serotypes III decreased from 27% (31/115) in 1994 to 0.9% (1/108) in 2005. A similar trend in MSSA strains was observed but serotype II strain was not detected in 2005. The antigenic shift and changes in the coagulase of S. aureus were confirmed.

Poster Ⅱ : Part B : Enzyme - Mechanism of Action & Regulation ; Studies on the Characterization of Mitochondrial Cytochrome c Oxidase in Lentinus edodes ( Berk . ) Sing

황수명,민태진 한국생화학분자생물학회 (구 한국생화학회) 1991 추계학술대회집 Vol.- No.-

Polyacrylamid Gel 전기영동법을 이용한 K.pneumoniae의 가용성 단백질 특성에 관한 연구

황선철,황수명 高麗大學校 倂設 保健大學 保健科學硏究所 1983 保健科學論集 Vol.13 No.1

In a series of 12 strains of Klebsiella pneumoniae isolated from clinical specimens, characterization of soluble cell proteins of strains was studied using polyacrylamide gel electrophoresis. The species specific is given mostly by major protein bands. Different gel patterns were observed according to the sources of stains and with characteristic results obtained through their biotypings, the variant strains also were confirmed.

농촌계획지원용 지역자원평가시스템 구축(I) - 자원평가 구성요소의 목표체계 구축 -

최수명,황한철 한국농촌계획학회 1997 농촌계획 Vol.3 No.1

Korean societies have been experiencing the wholesale structural changes in the rapid currents of recent openness, globalization and democratization, which effect much more heavily in rural areas than in urban areas, so rural recomposition works being an important national concern. In order to systematically reconstruct the rural structure, the decision makers, with a four step hierarchy of rural resident-residents group-community-region, should be endowed with the objective judgement on basic elements of resource potentialities under their control. In this process. rational resource evaluation works would be firstly necessiated from expert groups. Based on the view mentioned above, this study principally aimed at developing a rational evaluation framework for rural resources. For that objective, the first step of the study pigeonholed the total resources items identifiable in rural areas from the existing study results, spatial planning and field surveying data. After then, using the formalized classification criteria of resources items, a tentative goal system for rural resources evaluation was proposed and the final one determined through expert-group checking. The results obtained during the study are summarized as follows ; 1. Using the existing examples of resources identification/classification and the basic data list for county-level development planning as the principal reference ones, total rural resources elements were classified into 3 constituent units : land, natural environment and human resources, which correspond to places to work, to play and to live, respectively, as 3 constituent ones of life-supporting space. 2. Three characteristic areal types were adopted to represent the total rural areas : lowland, upland and seashore areas, and also 3 practical use types to represent the objectives of resources evaluation systems : for land use planning, natural conservation policy and village improvement planning. Thus 9 different types of goal system for resources evaluation were developed(each system by 3 areal typesX3 practical use types) 3. Each goal system has 3-tier classification steps from the higher, middle and lower one. The higher and middle steps should contain equally applicable components to all the rural areas, of which allowable number being around 3 and 4 respectively. However the lower step would contain detailed sub-components changeable to areal characteristics of which allowable number being around 7.

너구리의 狂犬病 發生 症例 報告

黃義卿,金哉勳,裵洙明,朴鍾元,禹鍾泰,尹用德 한국수의공중보건학회 1994 예방수의학회지 Vol.18 No.4

마을개발계획의 주민참여형 의사결정방법 - 영국의 마을평가사업을 중심으로 -

황한철(Han Cheol Hwang),최수명(Soo Myung Choi) 한국농촌계획학회 2001 농촌계획 Vol.7 No.2

A village appraisal is a study of the village, carried out by the people who live there as a basis for influencing its future. It is an exciting opportunity for a village to take a good look at itself, and it is a project in which everyone can be involved. It involves gathering factual information and the opinions of local people, so as to build up an overall picture of the village. An appraisal is a questionnaire survey carried out by and for the local community. It aims to identify local characteristics, problems, needs, threats, strengths and opportunities. It is a means of taking stock of the community and of creating a sound foundation of awareness and understanding on which to base future community action. Over the past 30 years more than 2,000 village appraisals have been undertaken in rural Britain. This research examined 48 village appraisals which had been carried out in England between 1985 and 1999. The suggested strengths and weaknesses of the village appraisals are: the target of the whole population in an area; the wide-ranging in its coverage of issues; well linked to a county-wide support agency; a good tool for identifying needs and wants, and local priorities; a good tool of local democracy and of increasing self-confidence in individuals and the whole community; not an exhaustive survey; very labour intensive; too little coverage of issues relating to the local economy; the lack of any input by professional planners; and so forth.

농촌계획지원용 지역자원평가시스템 구축 (4) - 사례지역 적용연구 -

최수명(Soo Myung Choi),한경수(Kyung Soo Han),황한철(Han Cheol Hwang) 한국농촌계획학회 1999 농촌계획 Vol.5 No.1

N/A

농촌계획지원용 지역자원평가시스템 구축 ( 3 ) - 농촌자원평가를 위한 평가등급기준 설정 -

최수명,한경수,황한철 ( Soo Myung Choi,Kyung Soo Han,Han Cheol Hwang ) 한국농촌계획학회 1998 농촌계획 Vol.4 No.1