탐진강의 총량규제를 위한 오염원 별 수계.행정구역 허용부하량 삭감부하량 할당에 관한 연구

황금록,황대호,백도현,이홍근 한국환경보건학회 2004 한국환경보건학회지 Vol.30 No.5

This study is to calculate Allocation of Pollution Discharges by administrative region for the TMDL (Total Maximum Daily Load) on Tamjin River. TMDL has the water quality target and value (BOD5, 1 ppm) and is calculated by the QUAL2E model. The expected TMDL for Tamjin River is 1,532,360 kg/day. The calculation showed that the main pollutants are due to the non-point sources in Tamjin River and the aqua-farms are another important sources near the bay. And sources from population and livestock should be reduced, especially aqua-farm source should be managed and eliminated first which is over 14,000 ton/day.

우리나라 하ㆍ폐수처리시설의 메탄(CH₄)과 아산화질소(N₂O) 배출량 산정 연구

황금록,김현선,이승묵,조경덕 한국대기환경학회 2007 한국대기환경학회 학술대회논문집 Vol.- No.-

환경, 에너지 그리고 순환 : 포스터 ; 지구환경 : 우리나라 하,폐수처리시설의 메탄(CH4)과 아산화질소(N2O) 배출량 산정 연구

황금록,김현선,이승묵,조경덕 한국폐기물자원순환학회(구 한국폐기물학회) 2007 공동 학술대회 초록집 Vol.2007 No.1

탐진강의 총량규제를 위한 오염원별 수계${cdot}$행정구역 허용부하량과 삭감부하량 할당에 관한 연구

황금록,황대호,백도현,이홍근,Hwang Kum-Lok,Hwang Dae-Ho,Paik Do-Hyeon,Lee Hong-Keun 한국환경보건학회 2004 한국환경보건학회지 Vol.30 No.5

This study is to calculate Allocation of Pollution Discharges by administrative region for the TMDL (Total Maximum Daily Load) on Tamjin River. TMDL has the water quality target and value ($BOD_5$, 1 ppm) and is calculated by the QUAL2E model. The expected TMDL for Tamjin River is 1,532,360 kg/day. The calculation showed that the main pollutants are due to the non-point sources in Tamjin River and the aqua-farms are another important sources near the bay. And sources from population and livestock should be reduced, especially aqua-farm source should be managed and eliminated first which is over 14,000 ton/day.

Vancomycin 의존성 장구균의 미생물학적 특성과 역학적 특성

황금록,성흥섭,김미나,남궁승,윤남섭 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.4

Background : Vancomycin-dependent enterococci (VDE) are clinically equivalent to vancomycinresistant enterococci (VRE), but more difficult to detect. This study was purposed to characterize VDE microbiologically and epidemiologically. Methods : The patients from whom VDE were detected from April 2007 to March 2008 were investigated. For available isolates, minimal inhibitory concentrations (MICs) of and the levels of dependence on vancomycin and teicoplanin were measured by E test (AB Biodisk, Sweden), and a test for reversion of VDE to non-dependent VRE (NDVRE) and pulsed field gel electrophoresis (PFGE) were performed. Patients’ demographic and clinical findings were reviewed via electronic medical records. Results : VDE were recovered from 6 (2.2%) of 272 patients carrying VRE during this study period. All patients were already colonized or infected by VRE and treated with vancomycin for 13 to 107 days. VDE were isolated from pleural fluid (one), urine (four), and stool (one). All isolates carried vanA with vancomycin MICs of >256 μg/mL, but two of them had intermediate susceptibilities to teicoplanin. Because 4 VDE isolates were reverted to NDVRE with single passage, vancomycin dependence was measurable for only two isolates as equal and above 0.064 and 0.5 μg/mL respectively, and was reverted after 5 and 7 passages, respectively. Six VDE isolates showed no related clones in PFGE analysis, and 3 of 4 available pairs of initial VRE isolates and subsequent VDE isolates were identical clones. Conclusions : VDE were not rare and seemed to emerge independently from VRE with a prolonged use of vancomycin. Vancomycin-dependence was reverted within several passages. 배경 : Vancomycin-dependent enterococci (VDE)는 vancomycin- resistant enterococci (VRE)와 유사한 임상상을 보 이지만 검출하기 더 어렵다. 본 연구에서는 VDE의 미생물학적 특성과 역학적 특성을 살펴보았다. 방법 : 2007년 4월부터 2008년 3월까지 VDE가 분리된 환자 를 대상으로 하였다. E test (AB Biodisk, Sweden)법으로 vancomycin과 teicoplanin에 대한 최소억제농도와 의존성을 정량 적으로 측정하였고, 의존성 환원검사와 pulsed field gel electrophoresis (PFGE)를 시행하였다. 전자의무기록을 통해 환자 의 임상 소견을 확인하였다. 결과 : 연구 기간 동안 VRE가 분리된 환자 272명 중 6명(2.2 %)에서 VDE가 검출되었다. 모두 VDE가 분리되기 전에 VRE에 의한 감염이 있거나 집락화되어 있었고 13-107일간 vancomycin 치료를 받았다. VDE는 흉수 1검체와 소변 4검체, 대변 1검 체에서 분리되었고 모두 vanA형이었다. Vancomycin 최소억 제농도는 모두 256 μg/mL 이상이었고 2주는 teicoplanin에 중등도 감수성이었다. 2주의 VDE에서 vancomycin 의존성은 0.064, 0.5 μg/mL로 측정되고 각각 5번, 7번의 계대배양 후 내성으로 전환되었다. 다른 4주는 한 번의 계대배양 후 vancomycin 의존성이 환원되었다. VDE 6주는 모두 서로 다른 PFGE 양상을 보였으며, 4명의 환자에서 이전에 분리된 VRE와 VDE 쌍 중 3쌍의 VRE와 VDE 쌍은 각각 동일한 클론이었다. 결론 : VDE의 발생빈도는 드물지 않았고, VRE가 분리되는 환자에서 장기간 vancomycin에 노출되었을 때 독립적으로 발 생하였다. Vancomycin 의존성은 수 차례의 계대배양 후 내성 으로 환원되었다.

우리나라 하·폐수처리시설의 메탄(CH₄)과 아산화질소(N₂O) 배출량 산정 연구

황금록,김현선,이승묵,조경덕 대한환경공학회 2007 대한환경공학회 학술발표논문집 Vol.2007 No.12

CD5 음성 만성림프구백혈병 1예

황금록,박찬정,박서진,장성수,허주영,지현숙,차충환,서철원 대한혈액학회 2009 Blood Research Vol.44 No.4

Neoplastic lymphoid cells of chronic lymphocytic leukemia (CLL) typically co-express CD5 and CD23. CD5-negative CLL is a rare variant of CLL; only 1 case of it has been reported in Korea. We describe a case of CD5-negative CLL. A 48-year-old female complained of a palpable neck mass that had been present for over 1 year. The initial WBC count was 7,300/μL, with 69% lymphocytes. A CT scan revealed multiple enlarged lymph nodes, both of each in the neck, axilla, and common iliac areas. The athologic results of the cervical lymph node was consistent with small lymphocytic lymphoma, of which tumor cells do not express CD5. In a bone marrow study, neoplastic lymphoid cells comprise 34.8% of all nucleated cells, which showed small size, round nuclei with clumped chromatin, and sparse cytoplasm. Immunophenotyping of small lymphoid cells displayed phenotypes that were CD45-, CD23-, CD20-, and CD19-positive, but CD5-negative. The patient was diagnosed with CD5-negative CLL, and has been followed up for 2.5 years after chemotherapy.

유세포검사에 의한 림프구아형검사에서 Gating 시 CD45의 필요성 및 NK세포 측정 시 CD16의 중요성

최영현,심효은,박찬정,한상희,황금록,장성수,지현숙 대한진단검사의학회 2013 Laboratory Medicine Online Vol.3 No.2

Background: Clinical and Laboratory Standards Institute (CLSI) guidelines (H42-A2) recommend the “CD45/SSC” gating method for assays on lymphocyte subset enumeration and CD16 exclusion for assays enumerating NK cells. In contrast, the Flow Cytometry Checklist (06/17/2010) of the College of American Pathology does not recommend a specific lymphocyte gating method, but recommends the correction of lymphocyte subset results for lymphocyte gate purity. Methods: We compared lymphocyte subset results of EDTA-treated blood from 102 patients with various diseases and 12 normal controls, using 3 lymphocyte gating methods (CD45/SSC, FSC/SSC, and lymphocyte gate purity correction after FSC/SSC gating), and assessed the proportion of CD56-/CD16+ NK cells within the total NK cell population. Results: Lymphocyte gate purity increased as the percentage of lymphocytes increased. However, lymphocyte subsets that consistently showed high lymphocyte gate purity could not be identified. The purity of the T cell population differed significantly depending on the gating method used:CD45/SSC vs. FSC/SSC, P =0.027; CD45/SSC vs. gate purity correction after FSC/SSC, P =0.002. However, the lymphocyte gate purity correction after FSC/SSC gating did not significantly improve the accuracy of the lymphocyte subset enumeration assay using FSC/SSC gating. The subset of CD56-CD16+ NK cells, constituted an average of 17.1% of total NK cells. Patients had higher proportions of CD56-CD16+ NK cells (13.1-25.5%)than did the normal controls (9.52%). Conclusions: In flow cytometric assays to evaluate lymphocytic subsets, the CD45 is inevitable for lymphocyte gating, whereas the measurement of CD16 is essential for the evaluation of NK cell proportions. 배경: Clinical and Laboratory Standards Institute 가이드라인(H42-A2)에서는 림프구아형분석을 위하여 ‘CD45/SSC’ gating법을 사용하고 NK세포아형 측정 시 CD16를 사용하지 않고 CD56만을 사용할 것을 권장하고 있다. 또한 CAP의 유세포검사 체크리스트에서는 림프구아형검사 시 gating을 위하여 특정한 gating방법의 사용을 권장하지 않고, 단지 gate 순도에 따라 림프구아형검사의 결과를 교정할 것을 권장하고 있다. 방법: 102명의 환자군과 12명의 정상 대조군으로부터 EDTA 혈액을 채취하여 세 가지의 gating법(CD45/SSC, FSC/SSC, FSC/SSCgating 후 gate 순도를 이용한 교정)으로 림프구아형검사를 실시하여 그 결과를 각각 비교하였고, NK세포 가운데 CD56-/CD16+ NK세포아형의 백분율을 평가하였다. 결과: 림프구백분율이 증가할수록 림프구 gate의 순도가 증가하는 것을 알 수 있었으나 높은 림프구 순도를 나타내는 특정한 림프구백분율은 알 수 없었다. T세포의 경우 gating법에 따라 결과가달라졌는데(CD45/SSC와 FSC/SSC 비교 시 결과값의 차이P = 0.027, CD45/SSC와 FSC/SSC gating 후 림프구순도로 교정 비교 시 결과값의 차이 P = 0.002), FSC/SSC gating 후 림프구순도로교정하는 방법이 FSC/SSC gating법에 비해 정확도가 증가되지 않았다. CD56-CD16+ NK세포아형은 총 NK세포의 17.1% (평균)를차지하였으며 환자군(13.1-25.5%)에서 대조군(9.5%) 보다 더 높은비율을 차지하였다. 결론: 유세포검사에 의한 림프구아형 측정에서 CD45는 gating 시반드시 사용되어야 하고, NK세포 측정 시 CD16 사용이 필수적이다.

증례 : 감염 ; Campylobacter jejuni 감염으로 인한 패혈 유산 1예

나신 ( Shin Na ),나희경 ( Hee Kyong Na ),박현정 ( Hyun Jung Park ),박영훈 ( Young Hoon Park ),황금록 ( Keum Rock Whang ),박기호 ( Ki Ho Park ),우준희 ( Jun Hee Woo ) 대한내과학회 2011 대한내과학회지 Vol.81 No.3

31세 초임부가 발열과 두통으로 내원하여 임신 중삼분기의 패혈 유산을 진단받았고 혈액배양 검사에서 C. jejuni가 동정되었다. 태반의 병리 소견에서 융모 사이 농양과 함께 급성 융모염이 관찰되었으며 태아 임신 중절과 항생제 사용으로 임상증상이 호전되었다. C. jejuni는 사람에서 발생하는 패혈 유산의 드문 원인균이고 아직까지 우리나라에서 보고 된 예가 없어 문헌고찰과 함께 보고하는 바이다. Campylobacter jejuni was isolated from the blood of a 31-year-old woman who suffered a mid-trimester septic abortion with fever and headache. Histologically, evidence of septic abortion was found, with an intervillous abscess and acute villitis. Complete clinical recovery followed termination of the pregnancy and the use of antibiotics. C. jejuni is an unusual cause of septic abortion in humans. To our knowledge, no case has been reported in Korea. (Korean J Med 2011;81:408-411)