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Genistein이 섬유주세포의 생존과 일산화질소의 생성에 미치는 영향
홍정흠,김윤영,김재우,Jung Heum Hong,Yun Young Kim,Jae Woo Kim 대한안과학회 2011 대한안과학회지 Vol.52 No.8
Purpose: To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 1, and 10 μm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day. Results: Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 μm and for 1 day at 1 μm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC. Conclusions: Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures. J Korean Ophthalmol Soc 2011;52(8):970-974
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증례보고 = 황반박리가 동반된 시신경유두소와에서 레이저 없이 시행한 유리체절제술 후 장기관찰 1예
홍정흠,김윤영,Jung Heum Hong,Yun Young Kim 대한안과학회 2011 대한안과학회지 Vol.52 No.9
Purpose: To report a long term result of vitrectomy, gas tamponade without laser retinopexy for serous macular detachment associated with an optic disc pit (ODP). Case summary: A 13 year old boy with visual disturbance in the left eye showed serous macular detachment associated with an inferior temporal ODP. The abnormal vitreous strand over the optic disc implying vitreous traction and retinoschisis were revealed by the optical coherence tomography (OCT) examination. Pars plana vitrectomy after complete induction of posterior vitreous detachment without laser retinopexy, and gas tamponade with postoperative facedown positioning were performed. Complete retinal attachment occurred at 6 months after first operation but recurrent macula detachment occurred at 15 months after first operation. Additional gas tamponade resulted in successful retinal attachment for more than 2 years and visual improvement. Conclusions: Vitrectomy and gas tamponade without additional laser photocoagulation could be another option for the treatment of ODP maculopathy. But recurrent macular detachment might occur and simple gas tamponade was effective in this case. This result supports another factor in addition to vitreous traction may play a role in the development of the macular detachment associated ODP. Further studies are required to evaluate the effect of vitrectomy, gas tamponade without laser retinopexy for the treatment of ODP maculopathy. J Korean Ophthalmol Soc 2011;52(9):1114-1118
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섬유주세포에서 국소 탄산탈수효소억제제가 일산화질소의 생성에 미치는 영향에 대한 연구
홍정흠,김세은,김재우,Jeung Hum Hong,MD,Se Eun Kim,MD,Jae Woo Kim,MD,PhD 대한안과학회 2014 대한안과학회지 Vol.55 No.3
Purpose: To investigate and compare the effects of topical carbonic anhydrase inhibitors on the production and expression of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 10, and 100 μM dorzolamide and brinzolamide using serum-deprived media for 3 days. Production of nitric oxide was assessed with Griess assay. Expressions of eNOS mRNA were assessed with RT-PCR. Results: Both dorzolamide and brinzolamide increased the production of nitric oxide eNOS activity (p < 0.05). Dorzolamide had a more potent effect than brinzolamide on the production of nitric oxide and the expression of eNOS mRNA. Conclusions: Topical carbonic anhydrase inhibitors increased the production of nitric oxide, which was accompanied by increased eNOS activity. Dorzolamide had a more potent effect than brinzolamide on the production of nitric oxide and expression of eNOS mRNA in HTMC. The increased production of nitric oxide by topical carbonic anhydrase inhibitors involves mechanisms other than carbonic anhydrase inhibition. J Korean Ophthalmol Soc 2014;55(3):416-421
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혈청결핍 상태에서 크레아틴이 RGC-5세포의 생존에 미치는 영향
김재우,홍정흠,강선희,김윤영,Jae Woo Kim,Jung Heum Hong,Sun Hee Kang,Yun Young Kim 대한안과학회 2011 대한안과학회지 Vol.52 No.5
Purpose: To evaluate the protective effect of creatine on the survival of retinal ganglion cells after serum deprivation. Methods: RGC-5 cells were exposed to 5 mM creatine with serum-free media for 4 days. Cellular survival and mitochondrial respiratory activity were measured with MTT assay and resazurin assay, respectively. Degree of apoptosis was evaluated with vital staining using acridine orange/Hoechest 33342 and flow cytometric analysis using annexin/PI, respectively. Results: Creatine increased cellular survival of RGC-5 cells significantly after serum deprivation. Additionally, creatine increased mitochondrial respiratory activity and inhibited apoptosis of RGC-5 cells. Conclusions: The energy precursor creatine increased survival of retinal ganglion cells after serum deprivation. Creatine could be relevant for the cytoprotection of retinal ganglion cells. J Korean Ophthalmol Soc 2011;52(5):618-623
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