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This study reports a simple and quantita-tive analytical method for detecting cartilage oligome-ric matrix protein(COMP) using a lateral-flow immu-noassay(LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle(AuNP)-conjugated monoclonal antibody(Ab) probe for the quantitative detection of COMP in human synovial fluid(SF). The detection antibody was conjugated with the AuNP(~30nm) to enable detection, and the cap-ture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commer-cial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic de-tection range from 0.6 to 20μg/mL of COMP. The detection limit of the proposed LFI system was approx-imately 0.2μg/mL. The test results showed good cor-relation with those obtained using a conventional en-zyme-linked immunosorbent assay(ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is sui-table for osteoarthritis diagnosis using SF samples.
Golden ratio, defined as symbol φ (Phi) simply in the 20th century, was acknowledged as the most balanced beautiful ratio, so it has been used in architecture or pictures etc. Golden ratio is the significant figurative ratio deeply related to our actual lives but it is not explained why many people consider it cozy and stable even in today, technologically advanced. Some refutation that golden ratio is only mathematical theory but there are few cases to be applied to practice. First, it was examined that there are many cases to exaggerate a small number of coincidences with golden ratio by chance in spite of numerous ratios in the nature. Besides, there are many cases that proportions of rectangular objects such as name card, credit card or proportions such as HDTV and wide monitors etc are approximate values of golden ratio but it is hard to place under the category of golden ratio for there are big differences with golden ratio. Rather, it was confirmed that it is close to Geumgang ratio, Korean own figurative ratio. Geumgang ratio is also called harmonic proportion and has flexibility to fit well to the cultural atmosphere of modern society which respects diversity. In this point of view, it is considered the active promotion according to global age would be need on the basis of more in-depth study on Geumgang ratio, likewise examples of golden ratio in the Western world.
Glycolide monomer is a starting material to synthesize polyglycolide. The monomer was synthesized by a catalytic reaction with Fe2(SO4)3`9H2O at high temperature. The synthesized crude monomer was purified by recrystalyzation. The restaylzation solvent was ethyl acetate. The reaction yield was much better than other known method and the purity of the monomer was higher.
N-butyl-N-(4``-tricyanoethenyl)phenyl-4-(2`-methoxy)ethoxyaniline which is a magenta dye is synthesized by a new synthetic method. The compound was obtained by four steps, which are hydroxyethylation, O-methylation, N-butylation and tetracyanoethenylation. Ethylene carbonate was used for the hydroxyethylation, and the methylation was accomplished with dimethylsulfate. The yield of every step was over 90 % and the purity of final compound was over 98 %.
Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QDbased biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.
Macrocyclic compounds are very useful for various biological application. The various synthetic methods are known for the macrocyclic compounds. In this article, the new synthetic method is proposed to obtain 1,4,8,11-tetraazatetradecane. 1,3-Diaminopropane is used as a starting compound to obtain the cyclic compound. After cyclic amide was obtained, LiAlH4 was used to obtain the target compound by reduction.
Urease is a enzyme to hydrolyze urea to ammonia and carbamate. In this article, simplified urease was designed and synthesized. The synthesis of the urease was obtained by reaction of picolyl chloride and 1,4,8,11-tetraazacyclotetradecane at near 0 oC.
A known yellow dye was synthesized by a new synthetic method. The dye, an aniline derivative, was synthesized by a five step. The first was O-alkylation, and then N-alkylation, followed by formylation. The last step was the formation of double bond by reaction of malononitrile.
Amlodipine besylate is a hypotensive drug. Amlodipine besylate was synthesized from N-(2-hydroxyethyl)phthalimide by 3 steps. 4-(2-chlorophenyl)-3-ethoxycarbonyl-5-methoxycabonyl-6-methyl-2(2-phtalimide ethoxymethyl)-1, 4-dihydropyridine is a second intermediate for the amlodipine besylate. After amlodipine besylate was synthesized, the spectroscopic analysis of amlodipine besylate was underwent to take HPLC assay data.