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Anti - HCV 양성인 만성 간질환 환자의 간조직에서의 in situ hybridization 과 면역조직화학적 염색을 이용한 HCV 검출
홍경표(Kyung Pyo Hong),최성규(Sung Kyu Choi),박창수(Chang Soo Park),김세종(Sei Jong Kim) 대한내과학회 1998 대한내과학회지 Vol.55 No.2
N/A Objective: PCR is useful for detecting hepatitis C virus(HCV) RNA in serum and tissue, but this technique can not indicate the specific site of HCV localization. In situ hybridization is widely applied as an effective method to detect the positive signal in the preservation of histological architecture with high specificity. In situ detection of HCV genome and gene production is important to allow for identification of cellular tropism, obtaining clues to the subcellular site of viral replication, and defining host-viral interaction. The aim of this study is b> evaluate the usefulness of sandwich in situ hybridization (SISH) and immunohistochemistry (IHC) in biopsy specimen of patients men anti-HCV positive chronic liver disease. Methods: The cellular localization of HCV RNA in 28cases of anti-HCV positive chronic liver disease was studied by SISH and the results were compared with those obtained by IHC. Results: Positivity of IHC was 33.3% in chronic persitent hepatitis, 55.6% in chronic active hepatitis and 75.0% in liver cirrhosis. Positivity of SISH was 50.0% in chronic persitent hepatitis, 88.9% in chronic active hepatitis and 75.0% in liver cirrhosis. Positve staining, redish precipitate, was mainly noted in the cytoplasm of hepatocyte. Detection rate of HCV by SISH and IHC in 28 anti-HCV positive chronic liver disease is 78.6% and 53.6%, respectively. Conclusion: Detection of HCV in liver by SISH and IHC is an useful method. But detection of HCV RNA sequences by in SISH appears to be a more sensitive method than IHC(p<O.05).