모링가:두충 2:1 (g:g) 복합 조성물의 in vitro 항산화 및 항염 효능 연구

허석모,양진원,김종래,박미령,김태기,구세광,박수진 한의병리학회 2022 동의생리병리학회지 Vol.36 No.4

The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 μM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.

Levels of common salivary protein 1 in healthy subjects and periodontal patients

허석모,이솔,HongTao Wang,정정혁,오상욱 대한치주과학회 2016 Journal of Periodontal & Implant Science Vol.46 No.5

Purpose: Human saliva, as a vital part of the immune defense system, contains a number of distinct proteins and peptides. Recently human common salivary protein 1 (CSP1) has been identified as an abundant salivary protein and may play a role in promoting the binding of cariogenic bacteria to salivary pellicles. However, nothing else is known regarding the role of CSP1 in periodontology. The aim of this study was to quantify and compare CSP1 levels between healthy subjects and periodontal patients. Methods: This controlled clinical study was conducted in periodontally healthy individuals and patients with chronic periodontitis Chonbuk National University Hospital, with Institutional Review Board approval. Whole saliva samples were collected from 36 healthy subjects and 33 chronic periodontitis patients and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immune blotting were conducted to ensure that anti-CSP1 monoclonal antibody (mAb) binds to CSP1 in human saliva. A sandwich enzyme-linked immunosorbent assay (ELISA) system was house-fabricated using mAb-hCSP1#14 and mAb-hCSP1#4 as a capture and a detector mAb, respectively. The CSP1 concentrations in saliva from 36 healthy subjects and 33 periodontal patients were quantified using the CSP1 sandwich ELISA system, and the results were analyzed using the Student’s t-test. Results: Immunoblot analysis using mAb-hCSP1 as a probe confirmed that CSP1 in human saliva existed as a single band with a molecular weight of approximately 27-kDa. The quantification of CSP1 concentrations by CSP1 ELISA showed that the median values (25th to 75th percentiles) of periodontal patients and healthy subjects were 9,474 ng/mL (range, 8,434–10,139 ng/mL) and 8,598 ng/mL (range, 7,421–9,877 ng/mL), respectively. The Student’s t-test indicated the presence of a statistically significant difference between the 2 groups (P=0.024). Conclusions: The presence of a significant difference in CSP1 levels between healthy subjects and periodontal patients suggests that CSP1 may be a potential biomarker for the detection or screening of periodontitis patients.

대한민국 치과의료종사자의 코로나19 : 2년간 감염발생 현황분석 및 치과감염관리지침 최신지견

허석모 대한치과의사협회 2022 대한치과의사협회지 Vol.60 No.5

Purpose: The purpose of this study is to analyze the prevalence and infection status of COVID-19 confirmed patients among dental healthcare workers, and to suggest guidelines for dental infection control, management and prevention based on the latest literature. Method: Information registered in the Basic Epidemiological Survey of COVID-19 confirmed cases of dental healthcare workers(dentists, dental hygienists, dental technicians) from the Korea Disease Control and Prevention Agency for 23 months from February 2020 to December 2021 was analyzed. Results: In the case of dentists, the number of confirmed cases increased about 9 times from an average of 1.1 monthly in 2020 to an average of 9.8 monthly in 2021. The number of confirmed cases among dentists, dental hygienists, and dental technicians has increased, with a total cumulative number of 395 confirmed cases until 2021. The highest number of month - ly confirmed cases was reported 111 cases in December 2021. There were 19.2 times more confirmed cases (269 cases) caused by community groups or close contact than the number of confirmed cases (14 cases) in dental hospital settings. Conclusions: Dental healthcare workers should follow basic infection control for COVID-19 prevention in the dental environment. Moreover, dental healthcare workers should check the latest COVID-19 guidelines, COVID-19 guidelines and follow them strictly in dental clinics as well as in their local communities.

유방암 생존자들의 자가관리 경험

허석모,허나래 한국간호과학회 2016 한국간호과학회 학술대회 Vol.2016 No.10

Dental Image Data Generation for Instance Segmentation using Generative Adversarial Networks

허석모,정승준,곽현민,정용호,양성민,이용학,김성환 계명대학교 자연과학연구소 2023 Quantitative Bio-Science Vol.42 No.2

Deep learning techniques have begun applied to the practice of medicine. Deep learning may, for example, assist physicians by showing pathogenic structures within diseases, including cysts, tumors, or infected tissue, or by recognizing objects such as cellular nuclei, or tumorous liver growths. However, the development of diagnostic deep learning techniques has been hampered by a scarcity of clinical image data. This is particularly true in the dental field. Accordingly, we propose a method of generating dental images using Generative Adversarial Networks (GANs). In this paper, we compare the performance of a Mask R-CNN model for image segmentation trained on a dataset that included GAN-generated data against a Mask R-CNN model trained with a dataset that did not include GAN-generated data. Ultimately, we observed improved performance by the Mask R-CNN that employed our GAN-generated images.

유방암 생존자들의 자가관리에 대한 현상학적 연구

허석모(Seok-Mo Heo),허나래(Narae Heo) 한국산학기술학회 2016 한국산학기술학회논문지 Vol.17 No.12

본 연구는 현상학적 방법을 적용하여 유방암 생존자들의 비약물적 추후 관리 경험에 대한 본질적 구조와 의미에 대해 심도 있게 파악하는 것을 목적으로 한다. 이번 연구에서 총 참여자는 10명으로 S시 E병원 외래에 정기적으로 내원하는 유방암 생존자로 수술, 화학요법, 방사선요법을 완료한 자들이었다. 자료수집 기간은 2014년 8월부터 2015년 2월까지로 수집된 자료는 심층면담 후 Colaizzi의 방법 적용하여 분석하였다. 연구결과, 7개의 주제와 16개의 하위범주가 도출되었다. 7개의 주제는 ‘1. 증상을 완화하기 위해 용이한 방법을 적용함, 2. 변화된 신체 상태를 위해 움직이려 함, 3. 재발 방지를 위해 특정식용 작물을 섭취함, 4. 건강을 유지하기 위한 식생활 양식이 있음, 5. 보완대체요법에 대해 항상 예의주시함, 6. 주치의 처방외 필요한 치료가 있음, 7. 자연친화적인 환경에서의 삶을 지향함’ 이었다. 이번 연구는 유방암 생존자들이 치료 후에 일상생활에서 어떻게 추후관리를 하는지 심도있게 파악하는 데 도움을 줄 것이다. 나아가 본 결과는 향후 유방암 생존자들에게 실제 관리 양식에 근거한 실행 가능한 지침과 프로그램을 제공하는데 있어 기초자료로써 제공될 것이다. The purpose of this study was to understand the essential structure and meaning of self-management intervention for breast cancer survivors by using non-pharmacological approaches. The study participants were 10 breast cancer survivors who were completing cancer treatment that involved surgery, chemotherapy, and radiation therapy. Data collected between August 2014 and February 2015 at E Hospital in S city were analyzed by using Colaizzi"s phenomenological method. Outcomes were classified into seven essential themes: 1) application of comfort measures to alleviate ongoing symptoms, 2) movement to change physical conditions, 3) special herbal intake to prevent recurrence, 4) a specially designed diet plan for health self-management, 5) constant awareness of complementary and alternative medicine, 6) unmet needs treated by one"s own doctor"s prescription, and 7) future life toward a nature-friendly environment. The study results contribute to a deeper understanding of self-management interventions in the daily lives of Korean breast cancer survivors. In addition, results provide an essential resource, based on actual self-management styles, that will help survivors to obtain guidance and participate in appropriate programs.

유방암 환자의 디스트레스가 피로에 미치는 영향: 삶의 질 매개 효과를 중심으로

허석모(Seok-Mo Heo),허나래(Narae Heo) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.9

본 연구는 유방암 환자의 디스트레스가 피로에 미치는 영향을 확인 후, 디스트레스와 피로의 관계에서 삶의 질의 매개 효과를 파악하고자 하였다. 서울시에 있는 상급종합병원 1곳에 내원한 유방암 환자를 대상으로 하였으며, 2014년 2월부터 2014년 12월까지 디스트레스, 삶의 질, 피로에 관련된 문항에 응답한 자료 110부를 분석에 사용하였다. IBM SPSS statistics 26 프로그램을 사용하여 서술통계, t-test, ANOVA, Pearson 상관관계, 위계적 회귀분석을 적용하였고, Sobel test로 삶의 질 매개 효과를 검증하였다. 삶의 질은 디스트레스(r=-.41, p <.001)와 피로(r=-.61, p <.001)와 통계적으로 각각 유의한 음의 상관관계가 있었으며, 디스트레스와 피로는 유의한 양의 상관관계(r=.53, p <.001)가 있었다. 피로에 대한 디스트레스의 직접적인 영향은 유의미했으며(β= -.337, p <.001), 삶의 질을 통한 피로에 대한 디스트레스의 간접적인 영향이 유의미했다(Sobel test: Z=3.743, p <.001). 분석 결과, 디스트레스가 피로에 미치는 영향에 있어 삶의 질이 부분 매개 효과가 있음을 확인하였다. 따라서, 유방암 환자의 디스트레스가 피로에 미치는 영향을 감소시키기 위해서, 디스트레스의 원인을 확인하고 근거기반의 심리적 지원과 삶의 질을 향상을 위한 맞춤형 지지 프로그램을 개발하여 간호 중재에 적용할 필요가 있다. The purpose of this study was to identify the mediating effect of quality of life on the impact of distress on fatigue. The subjects in this study were breast cancer patients who visited a general hospital in Seoul, and 110 of data responding to questionnaires related to distress, quality of life and fatigue were used for the analysis. Descriptive statistics, t-test, analysis of variance (ANOVA) , Pearson correlation and hierarchical regression were applied using the SPSS WIN 26 program, and the quality of life mediating effect was verified with the Sobel test. Quality of life had a significant negative correlation with distress (r=-.41, p <.001) and fatigue (r=-.61, p <.001), respectively, but distress and fatigue showed a significant positive correlation (r=.53, p <.001). The direct effect of distress on fatigue was significant (β=-.337, p <.001), and the indirect effect of distress on fatigue through the quality of life was also significant (Sobel test: Z=3.743, p <.001). It was confirmed that the quality of life had a partial mediating effect on the influence of distress on fatigue. In order to reduce the effect of distress on fatigue in breast cancer patients, it is necessary to identify the causes of distress. A personalized support program should be developed, which can improve the patient’s evidence-based psychological support and quality of life for applying nursing interventions.

유방암 생존자의 자가관리 지지 경험

허석모(Heo, Seok-Mo),허나래(Heo, Narae) 한국성인간호학회 2016 성인간호학회지 Vol.28 No.4

롱코비드 시대 구강 건강 관리 : 코로나19와 치주 질환 연관성을 중심으로

정재석,허석모 대한치과의사협회 2022 대한치과의사협회지 Vol.60 No.7

The purpose of this study is to investigate the relationship between periodontal disease and coronavirus disease 2019 (COVID-19) as a respiratory disease, and to suggest its molecular biological mechanism. Oral bacteria and viruses can be aspirated into the respiratory tract and may cause respiratory diseases. The COVID-19 virus can inhabit, replicate and spread in the oral environment including dental plaque, saliva, and periodontal pockets. The COVID-19 virus can induce strong innate immunity and inflammatory responses by binding to the angiotensin-converting enzyme 2 receptors, which are abundantly present in the oral stratified epithelium in the oral cavity. The COVID-19 virus may activate inflammasome, one of inflammatory key factors in cell cytoplasm. In patients with periodontal disease, oral hygiene habits, professional oral cleaning, and plaque control management are effective for reducing the possibility of COVID-19 infection. This review is based on the evidences from COVID-19 related literature in the last two years.

Comparison of Salivary Stress Hormone Levels between Periodontitis Patients and Healthy Subjects in Korea

이솔,허나래,허석모 대한치과의사협회 2016 대한치과의사협회지 Vol.54 No.11

Objectives: Periodontitis is multifactorial disease mainly caused by microbial community. Recently, some research has been conducted to find other possible risk factors including stress hormones related to periodontitis. Psychological stress can affect the periodontal health by a variety of biological mechanisms. This study compared the stress hormone levels in healthy subjects and patients with periodontal disease using saliva in order to investigate the association between periodontitis and stress. Methods: The human saliva was collected from 38 periodontally healthy individuals and 34 patients with chronic periodontitis under Institutional Review Board. Their age was 20-60 years (40.3 10.45). From these samples, determination of salivary levels of cortisol and Dehydroepiandrosterone (DHEA) performed by enzyme immunoassay kit (Salimetrics Europe, Suffolk, UK). The independent t-test and Mann-Whitney test for trend was applied using IBM SPSS statistics version 12.0 Program to analyze statistically significant differences. Results: Salivary cortisol levels of periodontitis patients were higher than those levels of healthy subjects (P < 0.001), while salivary DHEA levels of periodontitis patients were not significantly different (P = 0.431). Salivary cortisol/DHEA ratio of periodontitis patients was higher than those levels of healthy subjects (P < 0.001). Conclusions: Our study demonstrates the high levels of cortisol concentrations and cortisol/DHEA ratio in saliva of periodontitis patients than those of healthy subjects. Since cortisol levels and cortisol/DHEA ratio can be significant factors related to the severity of periodontal disease, our study would be helpful for early diagnosis and treatment of periodontal disease.